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Aliases for ENTPD8 Gene

Aliases for ENTPD8 Gene

  • Ectonucleoside Triphosphate Diphosphohydrolase 8 2 3 5
  • E-NTPDase 8 3 4
  • EC 4 56
  • GLSR2492 2 3
  • Liver Ecto-ATP-Diphosphohydrolase 3
  • E-NTPDase 3
  • NTPDase-8 3
  • NTPDase 8 4
  • NTPDase8 4
  • EC 3.6.1 56
  • UNQ2492 3

External Ids for ENTPD8 Gene

Previous GeneCards Identifiers for ENTPD8 Gene

  • GC17M077585
  • GC09M137604
  • GC09M139448
  • GC09M140328
  • GC09M109793

Summaries for ENTPD8 Gene

GeneCards Summary for ENTPD8 Gene

ENTPD8 (Ectonucleoside Triphosphate Diphosphohydrolase 8) is a Protein Coding gene. Among its related pathways are Pyrimidine metabolism (KEGG) and Purine metabolism. Gene Ontology (GO) annotations related to this gene include hydrolase activity and nucleoside-diphosphatase activity. An important paralog of this gene is ENTPD2.

UniProtKB/Swiss-Prot for ENTPD8 Gene

  • Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolysis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP.

Additional gene information for ENTPD8 Gene

No data available for Entrez Gene Summary , CIViC summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for ENTPD8 Gene

Genomics for ENTPD8 Gene

GeneHancer (GH) Regulatory Elements for ENTPD8 Gene

Promoters and enhancers for ENTPD8 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH09I137441 Promoter/Enhancer 1.9 EPDnew Ensembl ENCODE 550.8 +0.0 44 0.9 ARID4B SIN3A ZBTB7B ZNF48 SLC30A9 GLIS2 ZNF213 KLF7 SP3 SP5 ENTPD8 NOXA1 DPH7 ANAPC2
GH09I137233 Promoter/Enhancer 2.7 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 2.3 +203.5 203510 9.9 CLOCK MLX FEZF1 DMAP1 YY1 ZNF213 E2F8 ZNF416 ZNF143 SP3 TUBB4B EHMT1 SDCCAG3 ANAPC2 NDOR1 DPH7 INPP5E LOC651337 MAN1B1 SNHG7
GH09I137422 Promoter/Enhancer 2.3 EPDnew Ensembl ENCODE dbSUPER 0.4 +18.1 18123 3.3 HDGF PKNOX1 ARNT ARID4B SIN3A ZNF2 ZBTB7B ZNF766 CBX5 ZNF213 NOXA1 EXD3 NPDC1 AJM1 CACNA1B ENTPD8
GH09I137445 Enhancer 1.1 Ensembl ENCODE 0.8 -3.7 -3719 1.1 CLOCK SIN3A ZNF2 IRF4 BRCA1 YY1 GLIS2 ZNF143 FOS ATF7 ENTPD8 MIR7114
GH09I137437 Enhancer 0.6 ENCODE dbSUPER 0.8 +4.7 4692 0.2 ZNF189 ZFP36 ENTPD8 NOXA1
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around ENTPD8 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the ENTPD8 gene promoter:

Genomic Locations for ENTPD8 Gene

Genomic Locations for ENTPD8 Gene
7,453 bases
Minus strand

Genomic View for ENTPD8 Gene

Genes around ENTPD8 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ENTPD8 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ENTPD8 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ENTPD8 Gene

Proteins for ENTPD8 Gene

  • Protein details for ENTPD8 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Ectonucleoside triphosphate diphosphohydrolase 8
    Protein Accession:
    Secondary Accessions:
    • A2BG17
    • Q6UVZ0

    Protein attributes for ENTPD8 Gene

    495 amino acids
    Molecular mass:
    53904 Da
    Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    No Data Available

    Alternative splice isoforms for ENTPD8 Gene


neXtProt entry for ENTPD8 Gene

Selected DME Specific Peptides for ENTPD8 Gene


Post-translational modifications for ENTPD8 Gene

Other Protein References for ENTPD8 Gene

Domains & Families for ENTPD8 Gene

Gene Families for ENTPD8 Gene

Human Protein Atlas (HPA):
  • Enzymes
  • Predicted membrane proteins
  • Predicted secreted proteins

Protein Domains for ENTPD8 Gene

Suggested Antigen Peptide Sequences for ENTPD8 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry



  • The transmembranous domains are involved in regulation of enzyme activity.
  • Belongs to the GDA1/CD39 NTPase family.
  • The transmembranous domains are involved in regulation of enzyme activity.
  • Belongs to the GDA1/CD39 NTPase family.
genes like me logo Genes that share domains with ENTPD8: view

Function for ENTPD8 Gene

Molecular function for ENTPD8 Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=81 uM for ATP {ECO:0000269 PubMed:17095758}; KM=137 uM for ADP {ECO:0000269 PubMed:17095758}; KM=480 uM for UTP {ECO:0000269 PubMed:17095758}; KM=241 uM for UDP {ECO:0000269 PubMed:17095758}; Vmax=790 nmol/min/mg enzyme with ATP as substrate {ECO:0000269 PubMed:17095758}; Vmax=163 nmol/min/mg enzyme with ADP as substrate {ECO:0000269 PubMed:17095758}; Vmax=1100 nmol/min/mg enzyme with UTP as substrate {ECO:0000269 PubMed:17095758}; Vmax=110 nmol/min/mg enzyme with UDP as substrate {ECO:0000269 PubMed:17095758};
UniProtKB/Swiss-Prot CatalyticActivity:
A nucleoside 5-triphosphate + 2 H(2)O = a nucleoside 5-phosphate + 2 phosphate.
UniProtKB/Swiss-Prot EnzymeRegulation:
Not inhibited by ARL 67156.
UniProtKB/Swiss-Prot Function:
Canalicular ectonucleoside NTPDase responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolysis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP.

Enzyme Numbers (IUBMB) for ENTPD8 Gene

Gene Ontology (GO) - Molecular Function for ENTPD8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005524 ATP binding IEA --
GO:0016787 hydrolase activity IEA --
GO:0017110 nucleoside-diphosphatase activity IEA,EXP 16752921
GO:0017111 nucleoside-triphosphatase activity IEA,EXP 17095758
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with ENTPD8: view
genes like me logo Genes that share phenotypes with ENTPD8: view

Animal Models for ENTPD8 Gene

MGI Knock Outs for ENTPD8:

Clone Products

No data available for Phenotypes From GWAS Catalog , Human Phenotype Ontology , miRNA , Transcription Factor Targets and HOMER Transcription for ENTPD8 Gene

Localization for ENTPD8 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ENTPD8 Gene

Cell membrane; Multi-pass membrane protein.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ENTPD8 gene
Compartment Confidence
plasma membrane 5
extracellular 3
nucleus 2
cytosol 2
peroxisome 1
endoplasmic reticulum 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (2)
  • Nucleoplasm (2)
  • Vesicles (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for ENTPD8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005886 plasma membrane TAS --
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane IEA --
genes like me logo Genes that share ontologies with ENTPD8: view

Pathways & Interactions for ENTPD8 Gene

genes like me logo Genes that share pathways with ENTPD8: view

Pathways by source for ENTPD8 Gene

Gene Ontology (GO) - Biological Process for ENTPD8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0009124 nucleoside monophosphate biosynthetic process IEA --
GO:0009133 nucleoside diphosphate biosynthetic process IEA --
GO:0034656 nucleobase-containing small molecule catabolic process TAS --
genes like me logo Genes that share ontologies with ENTPD8: view

No data available for SIGNOR curated interactions for ENTPD8 Gene

Drugs & Compounds for ENTPD8 Gene

(16) Drugs for ENTPD8 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Phosphoric acid Approved Pharma 0
Water Approved Pharma 0
Adenosine monophosphate Approved, Investigational Nutra 0
calcium Approved Nutra 0
5-Thymidylic acid Experimental Pharma 0

(7) Additional Compounds for ENTPD8 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0
  • Deoxy-TDP
  • Deoxythymidine 5'-diphosphate
  • dTDP
  • TDP
  • Thymidine 5'-diphosphate
  • 5'-IDP
  • IDP
  • Inosine 5'-(trihydrogen diphosphate)
  • Inosine 5'-(trihydrogen pyrophosphate)
  • Inosine 5'-diphosphate
inosine triphosphate
  • 2'-Inosine-5'-triphosphate
  • 5'-ITP
  • 5-ITP
  • Inosine 5
  • Inosine 5'-(tetrahydrogen triphosphate)
  • NFB Orthophosphate
  • O-Phosphoric acid
  • Ortho-phosphate
  • Orthophosphate (PO43-)
  • Orthophosphate(3-)
genes like me logo Genes that share compounds with ENTPD8: view

Transcripts for ENTPD8 Gene

Unigene Clusters for ENTPD8 Gene

Ectonucleoside triphosphate diphosphohydrolase 8:
Representative Sequences:

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for ENTPD8 Gene

No ASD Table

Relevant External Links for ENTPD8 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for ENTPD8 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for ENTPD8 Gene

mRNA differential expression in normal tissues according to GTEx for ENTPD8 Gene

This gene is overexpressed in Colon - Transverse (x20.4), Small Intestine - Terminal Ileum (x10.4), and Liver (x5.7).

Protein differential expression in normal tissues from HIPED for ENTPD8 Gene

This gene is overexpressed in Pancreatic juice (64.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for ENTPD8 Gene

Protein tissue co-expression partners for ENTPD8 Gene

NURSA nuclear receptor signaling pathways regulating expression of ENTPD8 Gene:


SOURCE GeneReport for Unigene cluster for ENTPD8 Gene:


Evidence on tissue expression from TISSUES for ENTPD8 Gene

  • Liver(4.3)
genes like me logo Genes that share expression patterns with ENTPD8: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for ENTPD8 Gene

Orthologs for ENTPD8 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for ENTPD8 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia ENTPD8 33 34
  • 99.19 (n)
(Bos Taurus)
Mammalia ENTPD8 33 34
  • 81.04 (n)
(Rattus norvegicus)
Mammalia Entpd8 33
  • 79.82 (n)
(Canis familiaris)
Mammalia ENTPD8 33 34
  • 79.74 (n)
(Mus musculus)
Mammalia Entpd8 33 16 34
  • 78.61 (n)
(Monodelphis domestica)
Mammalia ENTPD8 34
  • 62 (a)
(Ornithorhynchus anatinus)
Mammalia ENTPD8 34
  • 36 (a)
(Gallus gallus)
Aves ENTPD8L 33
  • 62.72 (n)
  • 52 (a)
-- 34
  • 50 (a)
-- 34
  • 45 (a)
(Anolis carolinensis)
Reptilia ENTPD8 34
  • 42 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia entpd8 33
  • 62.93 (n)
(Danio rerio)
Actinopterygii entpd8 33 34
  • 53.41 (n)
(Caenorhabditis elegans)
Secernentea ntp-1 34
  • 20 (a)
mig-23 34
  • 19 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes YND1 34
  • 17 (a)
thale cress
(Arabidopsis thaliana)
eudicotyledons AT4G19180 33
  • 41.38 (n)
(Oryza sativa)
Liliopsida Os10g0350500 33
  • 39.58 (n)
Species where no ortholog for ENTPD8 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for ENTPD8 Gene

Gene Tree for ENTPD8 (if available)
Gene Tree for ENTPD8 (if available)

Paralogs for ENTPD8 Gene

Paralogs for ENTPD8 Gene

(3) SIMAP similar genes for ENTPD8 Gene using alignment to 2 proteins:

genes like me logo Genes that share paralogs with ENTPD8: view

Variants for ENTPD8 Gene

Sequence variations from dbSNP and Humsavar for ENTPD8 Gene

SNP ID Clin Chr 09 pos Variation AA Info Type
rs1000188152 -- 137,438,540(-) T/C intron_variant
rs1000212286 -- 137,437,619(-) C/G intron_variant
rs1000215814 -- 137,441,844(-) C/T upstream_transcript_variant
rs1000306736 -- 137,434,545(-) C/T 3_prime_UTR_variant
rs1000425176 -- 137,434,695(-) A/C 3_prime_UTR_variant

Structural Variations from Database of Genomic Variants (DGV) for ENTPD8 Gene

Variant ID Type Subtype PubMed ID
dgv4178n106 CNV deletion 24896259
esv26196 CNV loss 19812545
esv2657589 CNV deletion 23128226
esv2739446 CNV deletion 23290073
esv2739447 CNV deletion 23290073
esv2739448 CNV deletion 23290073
esv2759721 CNV loss 17122850
esv3622034 CNV gain 21293372
nsv1049808 CNV gain 25217958
nsv1075700 CNV deletion 25765185
nsv1161918 CNV duplication 26073780
nsv524322 CNV loss 19592680
nsv616021 CNV loss 21841781
nsv6771 CNV insertion 18451855
nsv951208 CNV deletion 24416366

Variation tolerance for ENTPD8 Gene

Residual Variation Intolerance Score: 78.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.11; 61.17% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ENTPD8 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ENTPD8 Gene

Disorders for ENTPD8 Gene

Additional Disease Information for ENTPD8

No disorders were found for ENTPD8 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for ENTPD8 Gene

Publications for ENTPD8 Gene

  1. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. (PMID: 12975309) Clark HF … Gray A (Genome research 2003) 2 3 4 58
  2. Cloning, purification, and identification of the liver canalicular ecto-ATPase as NTPDase8. (PMID: 17095758) Fausther M … Sévigny J (American journal of physiology. Gastrointestinal and liver physiology 2007) 3 4 58
  3. Molecular cloning and characterization of expressed human ecto-nucleoside triphosphate diphosphohydrolase 8 (E-NTPDase 8) and its soluble extracellular domain. (PMID: 16752921) Knowles AF … Li C (Biochemistry 2006) 3 4 58
  4. An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. (PMID: 24275569) Bian Y … Zou H (Journal of proteomics 2014) 4 58
  5. The stability of chicken nucleoside triphosphate diphosphohydrolase 8 requires both of its transmembrane domains. (PMID: 20000380) Li CS … Knowles AF (Biochemistry 2010) 22 58

Products for ENTPD8 Gene

Sources for ENTPD8 Gene

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