Aliases for ENO4 Gene

Aliases for ENO4 Gene

  • Enolase 4 2 3 4 5
  • 2-Phospho-D-Glycerate Hydro-Lyase 3 4
  • Enolase Family Member 4 2 3
  • C10orf134 3 4
  • Chromosome 10 Open Reading Frame 134 2
  • Enolase-Like Protein ENO4 3
  • EC 4
  • AC023283.3 2
  • ENO4 5

External Ids for ENO4 Gene

Previous HGNC Symbols for ENO4 Gene

  • C10orf134

Previous GeneCards Identifiers for ENO4 Gene

  • GC10P118599
  • GC10P112237

Summaries for ENO4 Gene

GeneCards Summary for ENO4 Gene

ENO4 (Enolase 4) is a Protein Coding gene. Diseases associated with ENO4 include Immunodeficiency 24 and Male Infertility. Among its related pathways are Carbon metabolism and Glucose metabolism. Gene Ontology (GO) annotations related to this gene include protein heterodimerization activity and phosphopyruvate hydratase activity. An important paralog of this gene is ENO3.

UniProtKB/Swiss-Prot Summary for ENO4 Gene

No data available for Entrez Gene Summary , CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for ENO4 Gene

Genomics for ENO4 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for ENO4 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH10J116847 Promoter/Enhancer 1.9 EPDnew Ensembl ENCODE CraniofacialAtlas 250.7 +0.9 850 3 CHD2 ZBTB10 SP1 FEZF1 BRCA1 ZNF217 SIN3A REST ZNF341 ZBTB33 ENO4 lnc-HSPA12A-1 SHTN1 HSPA12A
GH10J116887 Enhancer 1 Ensembl ENCODE 11.5 +41.1 41071 6.2 ZNF217 ZEB1 CEBPB SIN3A ZNF639 MAX EP300 RXRA ZNF843 JUND lnc-HSPA12A-3 SHTN1 ENO4 HSALNG0080881 HSPA12A
GH10J116963 Enhancer 0.9 UCNEbase dbSUPER 10.3 +113.3 113253 0.3 ZSCAN23 INSM2 SP7 ZNF366 ZEB2 OSR2 POLR2A WT1 ZBTB44 lnc-HSPA12A-4 ENO4 ENSG00000287655 HSPA12A SHTN1
GH10J116826 Enhancer 0.8 Ensembl 11.4 -21.3 -21329 1.8 FEZF1 ZEB1 CTCF ZNF639 ZNF843 BCL11B PRDM6 ZBTB1 INSM2 ZNF24 HSALNG0080879 MIR9851 piR-57463 ENO4 RPL12P26 KCNK18 lnc-HSPA12A-1 HSPA12A RF00017-811 C10orf82
GH10J116961 Enhancer 0.8 UCNEbase dbSUPER 10.3 +113.6 113622 0.4 ZSCAN23 INSM2 SP7 ZNF366 ZEB2 POLR2A lnc-HSPA12A-4 ENO4 ENSG00000287655 HSPA12A SHTN1
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around ENO4 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for ENO4

Genomic Locations for ENO4 Gene

Latest Assembly
63,574 bases
Plus strand

Previous Assembly
(GRCh37/hg19 by Entrez Gene)
33,103 bases
Plus strand

(GRCh37/hg19 by Ensembl)
62,277 bases
Plus strand

Genomic View for ENO4 Gene

Genes around ENO4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ENO4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ENO4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ENO4 Gene

Proteins for ENO4 Gene

  • Protein details for ENO4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Enolase 4
    Protein Accession:
    Secondary Accessions:
    • A0A087WZY6
    • A6NI74
    • B8ZZN9
    • J3KNX1

    Protein attributes for ENO4 Gene

    625 amino acids
    Molecular mass:
    68465 Da
    Quaternary structure:
    • Interacts with ENO1 and AKAP4.

    Alternative splice isoforms for ENO4 Gene


neXtProt entry for ENO4 Gene

Post-translational modifications for ENO4 Gene

  • Synthesized as an approximately 70-kDa precursor, which then undergoes proteolytic cleavage to an approximately 60-kDa enzyme; HOATZ associates directly or indirectly with ENO4 to mediate this process before its transport to mature flagella.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for ENO4 Gene

Domains & Families for ENO4 Gene

Gene Families for ENO4 Gene

Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins

Protein Domains for ENO4 Gene

Suggested Antigen Peptide Sequences for ENO4 Gene

GenScript: Design optimal peptide antigens:
  • Enolase-like protein ENO4 (ENO4_HUMAN)

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the enolase family.
  • Belongs to the enolase family.
genes like me logo Genes that share domains with ENO4: view

Function for ENO4 Gene

Molecular function for ENO4 Gene

UniProtKB/Swiss-Prot Function:
May be required for sperm motility and function.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate; Xref=Rhea:RHEA:10164, ChEBI:CHEBI:15377, ChEBI:CHEBI:58289, ChEBI:CHEBI:58702; EC=; Evidence={ECO:0000250|UniProtKB:Q8C042};.

Enzyme Numbers (IUBMB) for ENO4 Gene

Phenotypes From GWAS Catalog for ENO4 Gene

Gene Ontology (GO) - Molecular Function for ENO4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000287 magnesium ion binding IEA --
GO:0003674 molecular_function ND --
GO:0004634 phosphopyruvate hydratase activity IEA,IBA 21873635
GO:0016829 lyase activity IEA --
genes like me logo Genes that share ontologies with ENO4: view
genes like me logo Genes that share phenotypes with ENO4: view

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ENO4

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for ENO4 Gene

Localization for ENO4 Gene

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ENO4 gene
Compartment Confidence
cytosol 4
mitochondrion 2
nucleus 2
plasma membrane 1
extracellular 1
cytoskeleton 1
endoplasmic reticulum 1
golgi apparatus 1
peroxisome 0

Subcellular locations from the

Human Protein Atlas (HPA)
  • Mitochondria (2)
  • Nucleoplasm (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for ENO4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000015 phosphopyruvate hydratase complex IEA,IBA 21873635
GO:0005575 cellular_component ND --
GO:0097228 sperm principal piece IEA --
genes like me logo Genes that share ontologies with ENO4: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for ENO4 Gene

Pathways & Interactions for ENO4 Gene

genes like me logo Genes that share pathways with ENO4: view

UniProtKB/Swiss-Prot A6NNW6-ENO4_HUMAN

  • Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

Interacting Proteins for ENO4 Gene

Gene Ontology (GO) - Biological Process for ENO4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006096 glycolytic process IEA --
GO:0008150 biological_process ND --
GO:0030317 flagellated sperm motility IEA --
GO:0032889 regulation of vacuole fusion, non-autophagic IBA 21873635
GO:0044782 cilium organization IEA --
genes like me logo Genes that share ontologies with ENO4: view

No data available for SIGNOR curated interactions for ENO4 Gene

Drugs & Compounds for ENO4 Gene

No Compound Related Data Available

Transcripts for ENO4 Gene

mRNA/cDNA for ENO4 Gene

1 NCBI additional mRNA sequence :
4 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ENO4

Alternative Splicing Database (ASD) splice patterns (SP) for ENO4 Gene

No ASD Table

Relevant External Links for ENO4 Gene

GeneLoc Exon Structure for

Expression for ENO4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for ENO4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for ENO4 Gene

This gene is overexpressed in CD4 Tcells (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for ENO4 Gene

Protein tissue co-expression partners for ENO4 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for ENO4

SOURCE GeneReport for Unigene cluster for ENO4 Gene:


Evidence on tissue expression from TISSUES for ENO4 Gene

  • Nervous system(3.1)
genes like me logo Genes that share expression patterns with ENO4: view

Primer products for research

No data available for mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for ENO4 Gene

Orthologs for ENO4 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for ENO4 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia ENO4 30 31
  • 99.2 (n)
(Bos Taurus)
Mammalia ENO4 30 31
  • 86.44 (n)
(Canis familiaris)
Mammalia ENO4 30 31
  • 85.04 (n)
(Rattus norvegicus)
Mammalia Eno4 30
  • 78.52 (n)
(Mus musculus)
Mammalia Eno4 30 17 31
  • 78.08 (n)
(Monodelphis domestica)
Mammalia ENO4 31
  • 33 (a)
(Ornithorhynchus anatinus)
Mammalia ENO4 31
  • 28 (a)
(Gallus gallus)
Aves ENO4 30 31
  • 62.57 (n)
(Anolis carolinensis)
Reptilia ENO4 31
  • 22 (a)
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia eno4 30
  • 58.5 (n)
(Danio rerio)
Actinopterygii eno4 30 31
  • 52.07 (n)
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes ERR2 31
  • 12 (a)
ERR1 31
  • 12 (a)
ERR3 31
  • 11 (a)
ENO1 31
  • 11 (a)
ENO2 31
  • 10 (a)
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 18 (a)
Species where no ortholog for ENO4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for ENO4 Gene

Gene Tree for ENO4 (if available)
Gene Tree for ENO4 (if available)
Evolutionary constrained regions (ECRs) for ENO4: view image

Paralogs for ENO4 Gene

Paralogs for ENO4 Gene

genes like me logo Genes that share paralogs with ENO4: view

Variants for ENO4 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for ENO4 Gene

SNP ID Clinical significance and condition Chr 10 pos Variation AA Info Type
rs201860890 Benign: not provided 116,876,178(+) C/T
NM_001242699.2(ENO4):c.1455C>T (p.Ile485=)

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for ENO4 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for ENO4 Gene

Variant ID Type Subtype PubMed ID
nsv474141 CNV novel sequence insertion 20440878
nsv477919 CNV novel sequence insertion 20440878

Variation tolerance for ENO4 Gene

Gene Damage Index Score: 2.58; 44.76% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ENO4 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ENO4 Gene

Disorders for ENO4 Gene

MalaCards: The human disease database

(2) MalaCards diseases for ENO4 Gene - From: COP and GCD

Disorder Aliases PubMed IDs
immunodeficiency 24
  • imd24
male infertility
  • infertility male
- elite association - COSMIC cancer census association via MalaCards
Search ENO4 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for ENO4

genes like me logo Genes that share disorders with ENO4: view

No data available for UniProtKB/Swiss-Prot and Genatlas for ENO4 Gene

Publications for ENO4 Gene

  1. Disruption of a spermatogenic cell-specific mouse enolase 4 (eno4) gene causes sperm structural defects and male infertility. (PMID: 23446454) Nakamura N … Eddy EM (Biology of reproduction 2013) 2 3
  2. Genetic Architecture of Primary Open-Angle Glaucoma in Individuals of African Descent: The African Descent and Glaucoma Evaluation Study III. (PMID: 30352225) Taylor KD … African Descent and Glaucoma Evaluation Study III Genomics Study Group (Ophthalmology 2019) 3
  3. Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei. (PMID: 30021884) Fasci D … Heck AJR (Molecular & cellular proteomics : MCP 2018) 3
  4. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. (PMID: 21873635) Gaudet P … Thomas PD (Briefings in bioinformatics 2011) 3
  5. The full-ORF clone resource of the German cDNA Consortium. (PMID: 17974005) Bechtel S … Schupp I (BMC genomics 2007) 4

Products for ENO4 Gene

Sources for ENO4 Gene