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This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has b... See more...
Aliases for ENO1 Gene
Aliases for ENO1 Gene
External Ids for ENO1 Gene
- HGNC: 3350
- NCBI Entrez Gene: 2023
- Ensembl: ENSG00000074800
- OMIM®: 172430
- UniProtKB/Swiss-Prot: P06733
Previous HGNC Symbols for ENO1 Gene
- ENO1L1
- MPB1
Previous GeneCards Identifiers for ENO1 Gene
- GC01M008541
- GC01M008732
- GC01M008517
- GC01M008630
- GC01M008855
- GC01M008843
- GC01M008921
- GC01M008070
Summaries for ENO1 Gene
-
This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
GeneCards Summary for ENO1 Gene
ENO1 (Enolase 1) is a Protein Coding gene. Diseases associated with ENO1 include Hashimoto Encephalopathy and Cancer-Associated Retinopathy. Among its related pathways are Glucose metabolism and Carbon metabolism. Gene Ontology (GO) annotations related to this gene include transcription corepressor activity. An important paralog of this gene is ENO2.
UniProtKB/Swiss-Prot Summary for ENO1 Gene
-
Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate (PubMed:29775581, PubMed:1369209). In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed:2005901, PubMed:10802057, PubMed:12666133, PubMed:29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed:12666133). Stimulates immunoglobulin production (PubMed:1369209).
-
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
Additional gene information for ENO1 Gene
- HGNC (3350)
- NCBI Entrez Gene (2023)
- Ensembl (ENSG00000074800)
- OMIM® (172430)
- UniProtKB/Swiss-Prot (P06733)
- Open Targets Platform(ENSG00000074800)
- Monarch Initiative
- Search for ENO1 at DataMed
- Search for ENO1 at HumanCyc
No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for ENO1 Gene
Genomics for ENO1 Gene
GeneHancer (GH) Regulatory Elements Pubs
Promoters and enhancers for ENO1 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association
Download GeneHancer data from 2017 publication |
Request up-to-date GeneHancer data (full dataset)
GeneHancers around ENO1 on the GeneHancer Hub at the UCSC Golden Path
Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for ENO1
- Top Transcription factor binding sites by QIAGEN in the ENO1 gene promoter:
-
- AML1a
- c-Ets-1
- CUTL1
- GATA-1
- PPAR-gamma1
- PPAR-gamma2
- RFX1
- Sox5
Genomic Locations for ENO1 Gene
Latest Assembly
- chr1:8,861,000-8,879,190
- (GRCh38/hg38)
- Size:
- 18,191 bases
- Orientation:
- Minus strand
Previous Assembly
- chr1:8,921,059-8,938,745
- (GRCh37/hg19 by Entrez Gene)
- Size:
- 17,687 bases
- Orientation:
- Minus strand
- chr1:8,921,061-8,939,308
- (GRCh37/hg19 by Ensembl)
- Size:
- 18,248 bases
- Orientation:
- Minus strand
Genomic View for ENO1 Gene
Genes around ENO1 on UCSC Golden Path with GeneCards custom track
- Cytogenetic band:
-
- 1p36.23 by HGNC
- 1p36.23 by Entrez Gene
- 1p36.23 by Ensembl
ENO1 Gene in genomic location: bands according to
Ensembl, locations according to GeneLoc
(and/or Entrez Gene and/or Ensembl if different)
Genomic Neighborhood
• Exon Structure
• Gene Density
RefSeq DNA sequence for ENO1 Gene
Proteins for ENO1 Gene
-
Protein details for ENO1 Gene (UniProtKB/Swiss-Prot)
- Protein Symbol:
- P06733-ENOA_HUMAN
- Recommended name:
- Alpha-enolase
- Protein Accession:
- P06733
- B2RD59
- P22712
- Q16704
- Q4TUS4
- Q53FT9
- Q53HR3
- Q658M5
- Q6GMP2
- Q71V37
- Q7Z3V6
- Q8WU71
- Q96GV1
- Q9BT62
- Q9UCH6
- Q9UM55
Protein attributes for ENO1 Gene
- Size:
- 434 amino acids
- Molecular mass:
- 47169 Da
- Cofactor:
- Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
- Quaternary structure:
-
- Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific (PubMed:18560153). ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding (PubMed:9308760). Isoform MBP-1 interacts with TRAPPC2B (PubMed:11134351). Interacts with ENO4 and PGAM2 (By similarity). Interacts with CMTM6 (PubMed:28813417).
- SequenceCaution:
-
- Sequence=AAA35698.1; Type=Frameshift; Evidence={ECO:0000305}; Sequence=AAA35698.1; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
- Miscellaneous:
-
- Used as a diagnostic marker for many tumors and, in the heterodimeric form, alpha/gamma, as a marker for hypoxic brain injury after cardiac arrest. Also marker for endometriosis. Antibodies against alpha-enolase are present in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease which occurs in the presence of systemic tumor growth, primarily small-cell carcinoma of the lung and other malignancies. Is identified as an autoantigen in Hashimoto encephalopathy (HE) a rare autoimmune disease associated with Hashimoto thyroiditis (HT). HT is a disorder in which destructive processes overcome the potential capacity of thyroid replacement leading to hypothyroidism.
- [Isoform MBP-1]: It is uncertain whether the alternative initiation site is at Met-94 or at Met-97.
Three dimensional structures from OCA and Proteopedia for ENO1 Gene
Protein Expression for ENO1 Gene
Selected DME Specific Peptides for ENO1 Gene
- P06733:
-
- TGQIKTG
- LLLKVNQIG
- ELLKTAI
- YPVVSIEDPFDQD
- VGDDLTV
- DDLTVTNP
- DSRGNPT
- QLADLYK
- VSLAVCKAGA
- NKTIAPA
- PDQLADLY
- KAKFAGR
- EVYHNLK
- RYMGKGV
- KTGAPCR
- GDEGGFAP
- GQIKTGAP
- DVAASEF
- LRIEEEL
- PSGASTG
- SRGNPTVE
- EKGVPLY
- FIADLVVGL
- IGMDVAAS
- FAGRNFRNP
- QEFMILP
- RSGETED
- EVILPVPAFNVINGGSHAGNKLAMQEFMI
- GKGVSKAV
- FGANAIL
- GANAILGVS
- KYNQLLRI
- GKYDLDFKSPDD
- EAMRIGAEVYH
- SHRSGET
- VTNPKRI
- NVGDEGG
- DGTENKS
- KYGKDATNVG
- GVPLYRH
- LELLKTAI
- RSERLAK
Post-translational modifications for ENO1 Gene
- ISGylated.
- Lysine 2-hydroxyisobutyrylation (Khib) by p300/EP300 activates the phosphopyruvate hydratase activity.
-
Ubiquitination at Lys5, Lys120, Lys28, Lys126, Lys54, Lys60, Lys64, Lys71, Lys81, Lys89, Lys193, Lys202, Lys221, Lys228, Lys233, Lys256, Lys262, Lys281, Lys285, Lys326, Lys335, Lys343, Lys358, Lys406, and Lys420
- NX_P06733 (NX_P06733-1)
- Modification sites at PhosphoSitePlus
Other Protein References for ENO1 Gene
Antibodies for research
- LifeSpan BioSciences ENO1 / Alpha Enolase Monoclonal Antibody and ENO1 / Alpha Enolase Antibody
- GeneTex Anti-ENO1 antibody and Anti-ENO1 antibody [N3C3]
- NSJ Bioreagents Alpha Enolase Antibody / ENO1 (Rabbit Monoclonal)
-
Custom Antibody ServicesOriGene Antibodies for ENO1
- Novus Biologicals Antibodies for ENO1
-
Abcam antibodies for ENO1
- Invitrogen Antibodies for ENO1 (AFLGC-ENO1)
- antibodies-online: Show 88 available ENO1 Antibodies ranked by validation data
-
Custom Antibody ServicesProSci Antibodies for ENO1
- enolase 1, (alpha) [13-583]
- enolase 1, (alpha) [16-771]
- enolase 1, (alpha) [28-068]
- enolase 1, (alpha) [28-069]
- enolase 1, (alpha) [28-773]
- enolase 1, (alpha) [31-235]
- enolase 1 [54-318]
- Alpha-enolase [62-302]
- Alpha-enolase [61-571]
- Alpha-enolase [61-572]
- enolase 1, (alpha) [70-916]
- enolase 1, (alpha) [XW-7416]
-
Santa Cruz Biotechnology (SCBT) Antibodies for ENO1
- Sino Biological Antibodies for ENO1
Protein products for research
- G Biosciences Human Enolase 1/ENO1/Alpha-enolase/MPB-1/NNE, Liquid
-
OriGene Purified Proteins for ENO1
- Search Origene for MassSpec and Protein Over-expression Lysates for ENO1
- Origene Custom Protein Services for ENO1
- Sino Biological Recombinant Proteins for ENO1
- Browse Sino Biological Cell Lysates
- ProSpec Recombinant Proteins for ENO1
- antibodies-online: Search results for available ENO1 related products ranked by validation data
-
Abcam proteins for ENO1
Assay products for research
- antibodies-online: Search results for available ENO1 related products ranked by validation data
Domains & Families for ENO1 Gene
Gene Families for ENO1 Gene
- HGNC:
- Human Protein Atlas (HPA):
-
- Cancer-related genes
- Enzymes
- Plasma proteins
- Predicted intracellular proteins
Protein Domains for ENO1 Gene
- InterPro:
- Blocks:
-
- Enolase
- ProtoNet:
Suggested Antigen Peptide Sequences for ENO1 Gene
- GenScript: Design optimal peptide antigens:
-
- Enolase (A4QMW8_HUMAN)
- Plasminogen-binding protein (ENOA_HUMAN)
- Enolase (Q96GV1_HUMAN)
- Enolase (Q9BT62_HUMAN)
Graphical View of Domain Structure for InterPro Entry
P06733- Family:
-
- Belongs to the enolase family.
Function for ENO1 Gene
Molecular function for ENO1 Gene
- UniProtKB/Swiss-Prot Function:
- Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate (PubMed:29775581, PubMed:1369209). In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed:2005901, PubMed:10802057, PubMed:12666133, PubMed:29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed:12666133). Stimulates immunoglobulin production (PubMed:1369209).
- UniProtKB/Swiss-Prot Function:
- MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
- UniProtKB/Swiss-Prot CatalyticActivity:
- Reaction=(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate; Xref=Rhea:RHEA:10164, ChEBI:CHEBI:15377, ChEBI:CHEBI:58289, ChEBI:CHEBI:58702; EC=4.2.1.11; Evidence={ECO:0000269|PubMed:1369209, ECO:0000269|PubMed:29775581};.
- UniProtKB/Swiss-Prot Induction:
- Induced in diffuse large cell lymphoma (DLCL) after treatment with the natural biological agent, Bryo1. Up-regulated in response to enterovirus 71 (EV71) infection (at protein level).
- UniProtKB/Swiss-Prot BiophysicochemicalProperties:
- pH dependence: Enolase activity is lost above pH 9.0. Immunoglobulin production stimulating activity is retained at pH 13.0. {ECO:0000269|PubMed:1369209};
- GENATLAS Biochemistry:
- enolase 1,alpha subunit,ubiquitous and embryonic precocious type (2-phospho-D-glycerate hydratase),glycolysis,energy pathway
Enzyme Numbers (IUBMB) for ENO1 Gene
Phenotypes From GWAS Catalog for ENO1 Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000287 | magnesium ion binding | IEA | -- |
| GO:0000977 | RNA polymerase II regulatory region sequence-specific DNA binding | IDA | 2005901 |
| GO:0001227 | DNA-binding transcription repressor activity, RNA polymerase II-specific | IDA | 2005901 |
| GO:0003677 | DNA binding | IEA | -- |
| GO:0003723 | RNA binding | HDA | 22658674 |
Phenotypes for ENO1 Gene
- MGI mutant phenotypes for ENO1:
- inferred from 1 alleles
- GenomeRNAi human phenotypes for ENO1:
-
- Increased vaccinia virus (VACV) infection
- no effect
- shRNA abundance <= 50%
- shRNA abundance <= 25%
- Decreased shRNA abundance (Z-score < -2)
- Increased shRNA abundance (Z-score > 2)
- Increased hepcidin::fluc mRNA expression
- Decreased viability in esophageal squamous lineage; decreased viability in non-small-cell lung cancer (NSCLC) lineage
- Decreased shRNA abundance
Animal Models for research
- Taconic Biosciences: Generate A Custom CRISPR Mouse Model For Your Study
CRISPR products for research
- Applied Biological Materials (abm): CRISPR Clones for ENO1 - Starting at $85 >
- * ENO1 CRISPR as ready-to-use vector or virus: Non-viral | Lenti- | Adeno- | AAV- | siRNA - Browse All
- * Cas9 Nucleases, Nickases, Null Mutants, and GFP tag also available.
-
OriGene CRISPR knockouts for ENO1
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for ENO1
-
Santa Cruz Biotechnology (SCBT) CRISPR for ENO1
miRNA for ENO1 Gene
- miRTarBase miRNAs that target ENO1
-
- hsa-miR-744-5p (MIRT037729)
- hsa-miR-615-3p (MIRT039693)
- hsa-miR-18a-3p (MIRT040892)
- hsa-miR-181d-5p (MIRT041167)
- hsa-miR-484 (MIRT041760)
- hsa-miR-378a-3p (MIRT043896)
- hsa-miR-125a-5p (MIRT045746)
- hsa-miR-222-3p (MIRT046821)
- hsa-miR-204-5p (MIRT047021)
- hsa-miR-92a-3p (MIRT049082)
- hsa-miR-1260b (MIRT052701)
- hsa-miR-3177-3p (MIRT052801)
miRNA products for research
Inhibitory RNAs for research
- Origene shrna, sirna, and RNAi products in human, mouse, rat for ENO1
- Browse OriGene Inhibitory RNA Products For ENO1
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ENO1
Clone products for research
- Applied Biological Materials (abm): Clones for ENO1 - Starting at $45 >
- * ENO1 as ready-to-use vector or virus: ORF | Lenti- | Adeno- | AAV- | Retro- | Protein Vector - Browse All
- * Viral Packaging Services and High Titer Production Services also available.
- Sino Biological Human cDNA Clone for ENO1
- Addgene plasmids for ENO1
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for ENO1
No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for ENO1 Gene
Localization for ENO1 Gene
Subcellular locations from UniProtKB/Swiss-Prot for ENO1 Gene
- Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
- [Isoform MBP-1]: Nucleus.
| Compartment | Confidence |
|---|---|
| plasma membrane | 5 |
| extracellular | 5 |
| nucleus | 5 |
| cytosol | 5 |
| cytoskeleton | 3 |
| mitochondrion | 3 |
| peroxisome | 2 |
| endoplasmic reticulum | 2 |
| endosome | 2 |
| lysosome | 2 |
| golgi apparatus | 1 |
- Cytosol (4)
- Plasma membrane (4)
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000015 | phosphopyruvate hydratase complex | IEA,IDA | 3529090 |
| GO:0005615 | extracellular space | HDA | 16502470 |
| GO:0005634 | nucleus | IEA,IC | 2005901 |
| GO:0005737 | cytoplasm | IEA,IDA | 15459207 |
| GO:0005829 | cytosol | TAS | -- |
Pathways & Interactions for ENO1 Gene
| SuperPathway | Contained pathways | ||
|---|---|---|---|
| 1 | Glucose metabolism |
Glycolysis and gluconeogenesis (short map)
.58
.52
|
.46
|
| 2 | Carbon metabolism | ||
| 3 | Metabolism |
.40
|
|
| 4 | Glucose / Energy Metabolism | ||
| 5 | HIF-1 signaling pathway | ||
Pathways by source for ENO1 Gene
4 BioSystems pathways for ENO1 Gene
5 Reactome pathways for ENO1 Gene
6 KEGG pathways for ENO1 Gene
1 GeneGo (Thomson Reuters) pathway for ENO1 Gene
- Glycolysis and gluconeogenesis (short map)
1 Cell Signaling Technology pathway for ENO1 Gene
UniProtKB/Swiss-Prot P06733-ENOA_HUMAN
- Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Interacting Proteins for ENO1 Gene
Selected Interacting proteins:
P06733-ENOA_HUMAN
ENSP00000234590
for ENO1 Gene via
UniProtKB
MINT
STRING
IID
GPS-Prot Interaction Network for ENO1
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000122 | negative regulation of transcription by RNA polymerase II | IDA | 2005901 |
| GO:0006094 | gluconeogenesis | TAS | -- |
| GO:0006096 | glycolytic process | IEA,IBA | 21873635 |
| GO:0009615 | response to virus | IEP | 16548883 |
| GO:0010756 | positive regulation of plasminogen activation | IMP | 12666133 |
Drugs & Compounds for ENO1 Gene
| Name | Status | Disease Links | Group | Role | Mechanism of Action | Clinical Trials |
|---|---|---|---|---|---|---|
| Copper | Approved, Investigational | Pharma | Target | 271 | ||
| Zinc | Approved, Investigational | Pharma | Target | 1687 | ||
| zinc acetate | Approved, Investigational | Pharma | Target | 0 | ||
| Zinc chloride | Approved, Investigational | Pharma | Target, binder | 0 | ||
| Zinc sulfate, unspecified form | Approved, Experimental | Pharma | Target, binder | 0 |
| Name | Synonyms | Role | CAS Number | PubChem IDs | PubMed IDs |
|---|
(1) ApexBio Compounds for ENO1 Gene
| Compound | Action | Cas Number |
|---|---|---|
| AP-III-a4 | 1177827-73-4 |
Drug products for research
- G Biosciences Human Enolase 1/ENO1/Alpha-enolase/MPB-1/NNE, Liquid
- ApexBio compounds for ENO1
Transcripts for ENO1 Gene
mRNA/cDNA for ENO1 Gene
- 3 REFSEQ mRNAs :
- 31 NCBI additional mRNA sequence :
- 16 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :
-
- ENST00000234590 1781 nts (view in UCSC)
- ENST00000414948 744 nts (view in UCSC)
- ENST00000464920 2548 nts (view in UCSC)
- ENST00000486051 536 nts (view in UCSC)
- ENST00000489867 637 nts (view in UCSC)
CRISPR products for research
- Applied Biological Materials (abm): CRISPR Clones for ENO1 - Starting at $85 >
- * ENO1 CRISPR as ready-to-use vector or virus: Non-viral | Lenti- | Adeno- | AAV- | siRNA - Browse All
- * Cas9 Nucleases, Nickases, Null Mutants, and GFP tag also available.
-
OriGene CRISPR knockouts for ENO1
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for ENO1
-
Santa Cruz Biotechnology (SCBT) CRISPR for ENO1
miRNA products for research
Inhibitory RNAs for research
- Origene shrna, sirna, and RNAi products in human, mouse, rat for ENO1
- Browse OriGene Inhibitory RNA Products For ENO1
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ENO1
Clone products for research
- Applied Biological Materials (abm): Clones for ENO1 - Starting at $45 >
- * ENO1 as ready-to-use vector or virus: ORF | Lenti- | Adeno- | AAV- | Retro- | Protein Vector - Browse All
- * Viral Packaging Services and High Titer Production Services also available.
- Sino Biological Human cDNA Clone for ENO1
- Addgene plasmids for ENO1
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for ENO1
| ExUns: | 1a | · | 1b | ^ | 2 | ^ | 3a | · | 3b | · | 3c | ^ | 4a | · | 4b | ^ | 5a | · | 5b | · | 5c | ^ | 6 | ^ | 7 | ^ | 8 | ^ | 9 | ^ | 10 | ^ | 11 | ^ | 12 | ^ | 13 | ^ | 14 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SP1: | - | - | - | ||||||||||||||||||||||||||||||||||||
| SP2: | - | - | - | - | - | - | |||||||||||||||||||||||||||||||||
| SP3: | - | ||||||||||||||||||||||||||||||||||||||
| SP4: | - | ||||||||||||||||||||||||||||||||||||||
| SP5: | |||||||||||||||||||||||||||||||||||||||
| SP6: |
Relevant External Links for ENO1 Gene
- GeneLoc Exon Structure for
- ENO1
Expression for ENO1 Gene
Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for ENO1 Gene
- Elite partner
Transcriptomic regulation report from SPP (The Signaling Pathways Project) for ENO1
SOURCE GeneReport for Unigene cluster for ENO1 Gene:
Hs.517145mRNA Expression by UniProt/SwissProt for ENO1 Gene:
P06733-ENOA_HUMAN
Tissue specificity:
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Evidence on tissue expression from TISSUES for ENO1 Gene
- Nervous system(5)
- Skin(5)
- Kidney(5)
- Lung(5)
- Liver(5)
- Eye(5)
- Blood(5)
- Intestine(4.9)
- Stomach(4.9)
- Pancreas(4.9)
- Bone(4.6)
- Thyroid gland(4.6)
- Muscle(4.5)
- Heart(4)
- Spleen(3.6)
- Lymph node(3.3)
- Bone marrow(3.2)
- Adrenal gland(2.8)
- Gall bladder(2.3)
Primer products for research
-
OriGene qPCR primer pairs for ENO1
-
OriGene qPCR primer pairs and template standards for ENO1
Gene Expression Assays for research
No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , Protein differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for ENO1 Gene
Orthologs for ENO1 Gene
This gene was present in the common ancestor of eukaryotes.
| Organism | Taxonomy | Gene | Similarity | Type | Details |
|---|---|---|---|---|---|
| Chimpanzee (Pan troglodytes) |
Mammalia | ENO1 30 31 |
|
OneToOne | |
| Cow (Bos Taurus) |
Mammalia | -- 31 |
|
OneToMany | |
| ENO1 30 |
|
||||
| -- 31 |
|
OneToMany | |||
| Mouse (Mus musculus) |
Mammalia | Eno1 17 31 |
|
OneToMany | |
| Gm5506 30 31 |
|
OneToMany | |||
| Eno1b 17 |
|
||||
| Platypus (Ornithorhynchus anatinus) |
Mammalia | ENO1 31 |
|
OneToOne | |
| Oppossum (Monodelphis domestica) |
Mammalia | -- 31 |
|
OneToMany | |
| -- 31 |
|
OneToMany | |||
| Rat (Rattus norvegicus) |
Mammalia | Eno1 30 |
|
||
| Dog (Canis familiaris) |
Mammalia | ENO1 31 |
|
OneToOne | |
| Chicken (Gallus gallus) |
Aves | ENO1 30 31 |
|
OneToOne | |
| Lizard (Anolis carolinensis) |
Reptilia | ENO1 31 |
|
OneToOne | |
| Tropical Clawed Frog (Silurana tropicalis) |
Amphibia | eno1 30 |
|
||
| MGC75746 30 |
|
||||
| African clawed frog (Xenopus laevis) |
Amphibia | eno1-prov 30 |
|
||
| Zebrafish (Danio rerio) |
Actinopterygii | eno1a 30 31 |
|
OneToOne | |
| wufi32b03 30 |
|
||||
| African malaria mosquito (Anopheles gambiae) |
Insecta | AgaP_AGAP007827 30 |
|
||
| Fruit Fly (Drosophila melanogaster) |
Insecta | Eno 30 31 32 |
|
OneToMany | |
| Worm (Caenorhabditis elegans) |
Secernentea | enol-1 30 31 |
|
OneToMany | |
| Baker's yeast (Saccharomyces cerevisiae) |
Saccharomycetes | ENO1 31 |
|
ManyToMany | |
| ENO2 31 33 |
|
ManyToMany | |||
| ERR3 31 |
|
ManyToMany | |||
| ERR2 31 |
|
ManyToMany | |||
| ERR1 30 31 |
|
ManyToMany | |||
| Thale Cress (Arabidopsis thaliana) |
eudicotyledons | ENO1 30 |
|
||
| Alicante grape (Vitis vinifera) |
eudicotyledons | Vvi.7421 30 |
|
||
| Barley (Hordeum vulgare) |
Liliopsida | Hv.12462 30 |
|
||
| Corn (Zea mays) |
Liliopsida | Zm.410 30 |
|
||
| Sea Squirt (Ciona savignyi) |
Ascidiacea | CSA.10219 31 |
|
OneToMany | |
| Sea Vase (Ciona intestinalis) |
Ascidiacea | Cin.4682 30 |
|
- Species where no ortholog for ENO1 was found in the sources mined by GeneCards:
-
- A. gosspyii yeast (Eremothecium gossypii)
- Actinobacteria (Mycobacterium tuberculosis)
- Alpha proteobacteria (Wolbachia pipientis)
- Amoeba (Dictyostelium discoideum)
- Archea (Pyrococcus horikoshii)
- Beta proteobacteria (Neisseria meningitidis)
- Bread mold (Neurospora crassa)
- Chromalveolata (Phytophthora infestans)
- Common water flea (Daphnia pulex)
- E. coli (Escherichia coli)
- Filamentous fungi (Aspergillus nidulans)
- Firmicute Bacteria (Streptococcus pneumoniae)
- Fission Yeast (Schizosaccharomyces pombe)
- Green Algae (Chlamydomonas reinhardtii)
- Honey Bee (Apis mellifera)
- K. Lactis Yeast (Kluyveromyces lactis)
- Loblloly Pine (Pinus taeda)
- Malaria Parasite (Plasmodium falciparum)
- Medicago Trunc (Medicago Truncatula)
- Moss (Physcomitrella patens)
- Orangutan (Pongo pygmaeus)
- Pig (Sus scrofa)
- Rainbow Trout (Oncorhynchus mykiss)
- Rice (Oryza sativa)
- Rice Blast Fungus (Magnaporthe grisea)
- Schistosome Parasite (Schistosoma mansoni)
- Sea Anemone (Nematostella vectensis)
- Sea Urchin (Strongylocentrotus purpuratus)
- Sorghum (Sorghum bicolor)
- Soybean (Glycine max)
- Stem Rust Fungus (Puccinia graminis)
- Sugarcane (Saccharum officinarum)
- Tomato (Lycopersicon esculentum)
- Toxoplasmosis (Toxoplasma gondii)
- Trichoplax (Trichoplax adhaerens)
- Wheat (Triticum aestivum)
Evolution for ENO1 Gene
- ENSEMBL:
- Gene Tree for ENO1 (if available)
- TreeFam:
- Gene Tree for ENO1 (if available)
- Aminode:
- Evolutionary constrained regions (ECRs) for ENO1: view image
Paralogs for ENO1 Gene
(4) SIMAP similar genes for ENO1 Gene using alignment to 4 proteins:
- ENOA_HUMAN
- A4QMW8_HUMAN
- K7EM90_HUMAN
- K7ERS8_HUMAN
Pseudogenes.org Pseudogenes for ENO1 Gene
Variants for ENO1 Gene
Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for ENO1 Gene
| SNP ID | Clinical significance and condition | Chr 01 pos | Variation | AA Info | Type |
|---|---|---|---|---|---|
| rs1306980664 | Likely Benign: not provided | 8,871,910(-) |
C/T NM_001428.5(ENO1):c.162G>A (p.Lys54=) |
SYNONYMOUS_VARIANT,FIVE_PRIME_UTR | |
| rs1356620931 | Likely Benign: not provided | 8,865,492(-) |
G/A NM_001428.5(ENO1):c.668-10C>T |
INTRON | |
| rs138375821 | Benign: not provided | 8,861,360(-) |
T/C NM_001428.5(ENO1):c.1305A>G (p.Ter435=) |
SYNONYMOUS | |
| rs2230874 | Benign: not provided | 8,865,415(-) |
G/A NM_001428.5(ENO1):c.735C>T (p.Asp245=) |
SYNONYMOUS | |
| rs11544513 | - |
p.Asn177Lys |
All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).
| Variant ID | Type | Subtype | PubMed ID |
|---|---|---|---|
| esv3585154 | CNV | loss | 21293372 |
| nsv1001865 | CNV | gain | 25217958 |
| nsv545345 | CNV | gain | 21841781 |
Residual Variation Intolerance Score:
18.5% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score:
0.94;
19.50% of all genes are more intolerant (likely to be disease-causing)
Additional Variant Information for ENO1 Gene
SNP Genotyping and Copy Number Assays for research
No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ENO1 Gene
Disorders for ENO1 Gene
| Disorder | Aliases | PubMed IDs |
|---|---|---|
| hashimoto encephalopathy |
|
|
| cancer-associated retinopathy |
|
|
| autoimmune disease |
|
|
| lymphocytic hypophysitis |
|
|
| non-herpetic acute limbic encephalitis |
|
|
Search ENO1 in MalaCards
View complete list of genes associated with diseases
Additional Disease Information for ENO1
- Genetic Association Database
- (GAD)
- Human Genome Epidemiology Navigator
- (HuGE)
- Atlas
- ATLAS of Genetics and Cytogenetics in Oncology and Haematology
- Open Targets Platform
- Open Targets Platform
No data available for UniProtKB/Swiss-Prot and Genatlas for ENO1 Gene
Publications for ENO1 Gene
- Molecular cloning and expression analysis of five novel genes in chromosome 1p36. (PMID: 9653645) Onyango P … Weith A (Genomics 1998) 2 3 4 23
- Structure of human alpha-enolase (hENO1), a multifunctional glycolytic enzyme. (PMID: 18560153) Kang HJ … Chung SJ (Acta crystallographica. Section D, Biological crystallography 2008) 3 4 23
- Inhibition of cell surface mediated plasminogen activation by a monoclonal antibody against alpha-Enolase. (PMID: 12666133) López-Alemany R … Félez J (American journal of hematology 2003) 3 4 23
- Proteomic analysis of human brain identifies alpha-enolase as a novel autoantigen in Hashimoto's encephalopathy. (PMID: 12297304) Ochi H … Kira J (FEBS letters 2002) 3 4 23
- A novel 16-kilodalton cellular protein physically interacts with and antagonizes the functional activity of c-myc promoter-binding protein 1. (PMID: 11134351) Ghosh AK … Ray RB (Molecular and cellular biology 2001) 3 4 23
ExternalLinks
Products for ENO1 Gene
- Custom Antibody ServicesOriGene Antibodies for ENO1
- Browse OriGene ELISA Kits
- Custom Assay Services
- OriGene Purified Proteins for ENO1
- Search Origene for MassSpec and Protein Over-expression Lysates for ENO1
- Origene Custom Protein Services for ENO1
- Origene shrna, sirna, and RNAi products in human, mouse, rat for ENO1
- Browse OriGene Inhibitory RNA Products For ENO1
- OriGene qPCR primer pairs for ENO1
- OriGene qPCR primer pairs and template standards for ENO1
- OriGene CRISPR knockouts for ENO1
- Custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
- Browse OriGene miRNA Products For ENO1
- Sino Biological Human cDNA Clone for ENO1
- Sino Biological Recombinant Proteins for ENO1
- Browse Sino Biological Cell Lysates
- Sino Biological Antibodies for ENO1
- Browse Sino Biological Assays
- Browse Recombinant Proteins
- Browse Antibodies
- Browse cDNA Clones
- Browse ELISA Kits
- Browse CRO Services
- High-throughput Antibody Production Service
- Protein Production Service
- Epitope Tag Antibodies & Reporter Protein Antibodies
- Lentiviral ORF Clones
- Novus Biologicals Antibodies for ENO1
- Novus Biologicals proteins and lysates for ENO1
- Browse Antibodies
- Browse Peptides and Proteins
- Browse Lysates
- Browse ELISA Kits
- Abcam antibodies for ENO1
- Abcam proteins for ENO1
- Find your target
- Browse Primary Antibodies
- Browse Conjugated Primary Antibodies
- Browse Secondary Antibodies
- Browse ELISA Kits
- Browse Matched Antibody Pairs
- Browse Proteins and Peptides
- Search Knockout (KO) Validated Antibodies
- Browse Monoclonal Antibodies
- Browse Recombinant Antibodies
- ApexBio compounds for ENO1
- VectorBuilder Custom and pre-made DNA vectors for ENO1 (ie. gene expression, shRNA knockdown, CRISPR)
- VectorBuilder Viral vectors for ENO1 (ie. lentivirus, AAV, adenovirus)
- VectorBuilder CRISPR vectors for ENO1 (ie. knockout, knockin, CRISPRa, CRISPRi)
- VectorBuilder Virus packaging for ENO1 CRISPR vectors (ie. lentivirus, AAV, adenovirus)
- VectorBuilder Stable cell line generation for ENO1
- Addgene plasmids for ENO1
- antibodies-online: Show 88 available ENO1 Antibodies ranked by validation data
- antibodies-online: Search results for available ENO1 related products ranked by validation data
- antibodies-online: Search results for available ENO1 related products ranked by validation data
- Santa Cruz Biotechnology (SCBT) Antibodies for ENO1
- Search Santa Cruz Biotechnology (SCBT) for ENO1 siRNA/shRNA
- Santa Cruz Biotechnology (SCBT) CRISPR for ENO1
- Custom Antibody ServicesProSci Antibodies for ENO1
- enolase 1, (alpha) [13-583]
- enolase 1, (alpha) [16-771]
- enolase 1, (alpha) [28-068]
- enolase 1, (alpha) [28-069]
- enolase 1, (alpha) [28-773]
- enolase 1, (alpha) [31-235]
- enolase 1 [54-318]
- Alpha-enolase [62-302]
- Alpha-enolase [61-571]
- Alpha-enolase [61-572]
- enolase 1, (alpha) [70-916]
- enolase 1, (alpha) [XW-7416]
- Search for ENO1 Proteins at ProSci
- Browse GeneCopoeia Cell Lines
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for ENO1
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for ENO1
- Search GeneCopoeia for miRNA 3'UTR Target clone products for ENO1
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ENO1
Sources for ENO1 Gene
- (1) GeneCards
- (2) HGNC
- (3) NCBI Entrez Gene
- (4) UniProtKB/Swiss-Prot
- (5) Ensembl
- (6) OMIM® (Updated: 05-Mar-2021)
- (7) GeneLoc
- (8) Gene Wiki
- (9) UCSC
- (10) PhosphoSitePlus
- (11) Cell Signaling Technology
- (12) GO
- (13) UniProtKB/TrEMBL
- (14) InterPro
- (15) ProtoNet
- (16) Blocks
- (17) MGI
- (18) IUBMB
- (19) KEGG
- (20) MINT
- (21) STRING
- (22) IntAct
- (23) Novoseek
- (24) PharmGKB
- (25) DrugBank
- (26) HMDB
- (27) ASD
- (28) SAGE
- (29) SOURCE
- (30) HomoloGene
- (31) PanEnsembl
- (32) euGenes
- (33) SGD
- (34) FlyBase
- (35) WormBase
- (36) Pseudogene
- (37) DGV
- (38) dbSNP
- (39) GenAtlas
- (40) HGMD
- (41) GAD
- (42) BGMUT
- (43) HuGE
- (44) Atlas
- (45) GenBank
- (46) HORDE
- (47) HUGE
- (48) IMGT
- (49) Leiden
- (50) miRBase
- (51) DME
- (52) OriGene
- (53) PubMed
- (54) R&D Systems
- (55) TGDB
- (56) Tocris
- (57) Abcam
- (58) Novus Biologicals
- (59) ProSpec
- (60) Sino Biological
- (61) GenScript
- (62) Qiagen
- (63) OCA
- (64) Proteopedia
- (65) MOPED
- (66) neXtProt
- (67) Reactome
- (68) GeneGo (Thomson Reuters)
- (69) DISEASES
- (70) SIMAP
- (71) GenomeRNAi
- (72) LifeMap
- (73) miRTarBase
- (74) MalaCards
- (75) Invitrogen
- (76) BitterDB
- (77) ESI-BIO
- (78) RefSeq
- (79) BioSystems
- (80) MaxQB
- (81) IUPHAR
- (82) BioGPS
- (83) Illumina
- (84) COMPARTMENTS
- (85) HOMER
- (86) PaxDb
- (87) ApexBio
- (88) Addgene
- (89) antibodies-online
- (90) CYP
- (91) NONCODE
- (92) TreeFam
- (93) PathCards
- (94) GeneReviews
- (95) GeneTex
- (96) Taconic Biosciences
- (97) GTEx
- (98) ProteomicsDB
- (99) SCBT
- (100) DGIdb
- (101) ClinicalTrials
- (102) RVIS
- (103) SIGNOR
- (104) diseasecard
- (105) NIH Rare Diseases
- (106) Orphanet
- (107) UMLS
- (108) GTR
- (109) Disease Ontology
- (110) MedlinePlus Genetics
- (111) MeSH
- (112) MedlinePlus
- (113) CDC
- (114) NINDS
- (115) NCBI Bookshelf
- (116) ClinVar
- (117) Gene Damage Index
- (118) HPO
- (119) UDN
- (120) VISTA
- (121) FANTOM5
- (122) ENCODE
- (123) ProSci
- (124) Horizon
- (125) The Signaling Pathways Project (SPP)
- (126) IID
- (127) VectorBuilder
- (128) SNPedia
- (129) BRCA Exchange
- (130) CIViC
- (131) ProteoGenix
- (132) dbSUPER
- (133) TISSUES
- (134) Gene ORGANizer
- (135) abm
- (136) Human Protein Atlas
- (137) GWAS Catalog
- (138) Monarch Initiative
- (139) DataMed
- (140) HumanCyc
- (141) UCNEbase
- (142) EPDnew
- (143) Applied Biosystems
- (144) GlyConnect
- (145) Aminode
- (146) GeneCopoeia
- (147) GPS-Prot
- (148) RNAcentral
- (149) LncRNADisease
- (150) LOVD
- (151) miR2Disease
- (152) DiseaseEnhancer
- (153) Boster Bio
- (154) Biorbyt
- (155) Biocompare
- (156) Mastermind
- (157) CTD^2 Dashboard
- (158) CraniofacialAtlas
- (159) GeneImprint
- (160) Synthego
- (161) ENA
- (162) GtRNAdb
- (163) LncBase
- (164) LncBook
- (165) LNCipedia
- (166) Modomics
- (167) PDBe
- (168) Rfam
- (169) SILVA
- (170) snOPY
- (171) SRPDB
- (172) GENCODE
- (173) TarBase
- (174) Open Targets Platform
- (175) 5SRRNADB
- (176) snoDB
- (177) MirGeneDB