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Aliases for DUT Gene

Aliases for DUT Gene

  • Deoxyuridine Triphosphatase 2 3 5
  • DUTP Pyrophosphatase 2 3 4
  • DUTP Diphosphatase 2 3
  • EC 3.6.1.23 4 56
  • DUTPase 3 4
  • Deoxyuridine 5-Triphosphate Nucleotidohydrolase, Mitochondrial 3
  • DUTP Nucleotidohydrolase 3

External Ids for DUT Gene

Previous GeneCards Identifiers for DUT Gene

  • GC15P044181
  • GC15P041669
  • GC15P046202
  • GC15P046339
  • GC15P046410
  • GC15P048623
  • GC15P025461

Summaries for DUT Gene

Entrez Gene Summary for DUT Gene

  • This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq, Jul 2008]

GeneCards Summary for DUT Gene

DUT (Deoxyuridine Triphosphatase) is a Protein Coding gene. Diseases associated with DUT include Cysticercosis. Among its related pathways are Pyrimidine metabolism (KEGG) and Drug metabolism - cytochrome P450. Gene Ontology (GO) annotations related to this gene include magnesium ion binding.

UniProtKB/Swiss-Prot for DUT Gene

  • This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Gene Wiki entry for DUT Gene

Additional gene information for DUT Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for DUT Gene

Genomics for DUT Gene

GeneHancer (GH) Regulatory Elements for DUT Gene

Promoters and enhancers for DUT Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH15I048330 Promoter/Enhancer 2 EPDnew Ensembl ENCODE 556.1 +1.0 990 3.8 HDGF FOXA2 ZFP64 ARID4B SIN3A FEZF1 DMAP1 ZNF2 YY1 POLR2B DUT ENSG00000259488 SLC12A1 LOC105370807 CEP152 FBN1 GC15P048420
GH15I049154 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE 22.4 +824.7 824657 3.1 HDGF FOXA2 PKNOX1 MLX ARNT ARID4B SIN3A FEZF1 DMAP1 IRF4 COPS2 GALK2 NDUFAF4P1 DTWD1 DUT ENSG00000259488 CEP152 FGF7 SECISBP2L FAM227B
GH15I049044 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE 10.7 +714.8 714802 2.8 MLX DMAP1 YY1 SLC30A9 ZNF213 E2F8 ZNF143 SP3 NFYC SSRP1 SECISBP2L DTWD1 CEP152 DUT SHC4 ENSG00000259488 RN7SL577P
GH15I048855 Enhancer 1 Ensembl ENCODE 18.7 +525.0 525006 1.2 CTCF RB1 ZBTB21 TEAD4 MZF1 FEZF1 RAD21 RFX5 TEAD3 GATA3 DUT ENSG00000259488 SHC4 EID1 CEP152
GH15I047735 Enhancer 0.9 ENCODE dbSUPER 18.7 -594.2 -594187 2.7 ZNF146 MXI1 JUN CEBPG RFX5 ZNF335 ATF7 NFE2L2 CHD2 TCF7L2 DUT ENSG00000259488 RPL7AP62 GC15P047767 SEMA6D
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around DUT on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the DUT gene promoter:

Genomic Locations for DUT Gene

Genomic Locations for DUT Gene
chr15:48,331,011-48,343,373
(GRCh38/hg38)
Size:
12,363 bases
Orientation:
Plus strand
chr15:48,623,208-48,635,570
(GRCh37/hg19)

Genomic View for DUT Gene

Genes around DUT on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
DUT Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for DUT Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for DUT Gene

Proteins for DUT Gene

  • Protein details for DUT Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P33316-DUT_HUMAN
    Recommended name:
    Deoxyuridine 5-triphosphate nucleotidohydrolase, mitochondrial
    Protein Accession:
    P33316
    Secondary Accessions:
    • A8K650
    • B4DPR5
    • O14785
    • Q16708
    • Q16860
    • Q6FHN1
    • Q6NSA3
    • Q96Q81

    Protein attributes for DUT Gene

    Size:
    252 amino acids
    Molecular mass:
    26563 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Homotrimer.
    Miscellaneous:
    • Each trimer binds three substrate molecules. The ligands are bound between subunits, and for each substrate molecule, residues from adjacent subunits contribute to the binding interactions.
    SequenceCaution:
    • Sequence=AAB71393.1; Type=Frameshift; Positions=29, 47; Evidence={ECO:0000305}; Sequence=AAB93866.1; Type=Frameshift; Positions=29, 47; Evidence={ECO:0000305}; Sequence=AAB94642.1; Type=Frameshift; Positions=29, 47; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for DUT Gene

    Alternative splice isoforms for DUT Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for DUT Gene

Selected DME Specific Peptides for DUT Gene

P33316:
  • AAGYDLYSAYDYTIP
  • GGFGSTG
  • YGRVAPRSGLA
  • GVIDEDYRGN

Post-translational modifications for DUT Gene

  • Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2. Phosphorylation in mature T-cells occurs in a cell cycle-dependent manner. Isoform 3 is not phosphorylated.
  • Ubiquitination at Lys63 and posLast=9191

Domains & Families for DUT Gene

Gene Families for DUT Gene

Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins

Suggested Antigen Peptide Sequences for DUT Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P33316

UniProtKB/Swiss-Prot:

DUT_HUMAN :
  • Belongs to the dUTPase family.
Family:
  • Belongs to the dUTPase family.
genes like me logo Genes that share domains with DUT: view

Function for DUT Gene

Molecular function for DUT Gene

GENATLAS Biochemistry:
dUTP pyrophosphatase,involved in DNA replication
UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=2.5 uM for dUTP {ECO:0000269 PubMed:8631816}; Note=for both isoform 2 and isoform 3.;
UniProtKB/Swiss-Prot CatalyticActivity:
dUTP + H(2)O = dUMP + diphosphate.
UniProtKB/Swiss-Prot EnzymeRegulation:
Phosphorylation is necessary for activity.
UniProtKB/Swiss-Prot Function:
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Enzyme Numbers (IUBMB) for DUT Gene

Phenotypes From GWAS Catalog for DUT Gene

Gene Ontology (GO) - Molecular Function for DUT Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000287 magnesium ion binding IBA --
GO:0003723 RNA binding IDA,HDA 22658674
GO:0004170 dUTP diphosphatase activity IBA,IEA --
GO:0005515 protein binding IPI 16189514
GO:0016787 hydrolase activity IEA --
genes like me logo Genes that share ontologies with DUT: view
genes like me logo Genes that share phenotypes with DUT: view

Animal Model Products

Inhibitory RNA Products

Clone Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for DUT Gene

Localization for DUT Gene

Subcellular locations from UniProtKB/Swiss-Prot for DUT Gene

Isoform 2: Nucleus.
Isoform 3: Mitochondrion.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for DUT gene
Compartment Confidence
extracellular 5
mitochondrion 5
nucleus 5
cytosol 3
peroxisome 2

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for DUT Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus TAS 8631816
GO:0005654 nucleoplasm IDA,TAS --
GO:0005737 cytoplasm IEA --
GO:0005739 mitochondrion TAS,IEA --
GO:0005829 cytosol IEA --
genes like me logo Genes that share ontologies with DUT: view

Pathways & Interactions for DUT Gene

genes like me logo Genes that share pathways with DUT: view

UniProtKB/Swiss-Prot P33316-DUT_HUMAN

  • Pathway: Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.

Gene Ontology (GO) - Biological Process for DUT Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001889 liver development IEA --
GO:0006139 nucleobase-containing compound metabolic process TAS 1325640
GO:0006226 dUMP biosynthetic process IEA --
GO:0006260 DNA replication TAS 1325640
GO:0009117 nucleotide metabolic process IEA --
genes like me logo Genes that share ontologies with DUT: view

No data available for SIGNOR curated interactions for DUT Gene

Drugs & Compounds for DUT Gene

(8) Drugs for DUT Gene - From: DrugBank, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Magnesium Approved Nutra 0
Phosphoric acid Approved Pharma 0
Water Approved Pharma 0
1-{(2S,5S)-4-FLUORO-5-[(TRITYLOXY)METHYL]TETRAHYDROFURAN-2-YL}PYRIMIDINE-2,4(1H,3H)-DIONE Experimental Pharma Target 0
Deoxyuridine-5'-Diphosphate Experimental Pharma Target 0

(16) Additional Compounds for DUT Gene - From: Novoseek and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
pyrophosphate
  • (4-)Diphosphoric acid ion
  • (P2O74-)Diphosphate
  • Diphosphate
  • Diphosphoric acid
  • PPi
14000-31-8
Deoxyuridine triphosphate
  • 2'-Deoxyuridine 5'-triphosphate
  • 2'-Deoxyuridine-5'-triphosphorate
  • 2'-Deoxyuridine-5'-triphosphoric acid
  • 2'-Deoxyuridine-5'-triphosphoric acid = dUTP
  • Deoxy-UTP
1173-82-6
dUMP
  • 2'-Deoxy-5'-uridylate
  • 2'-Deoxy-5'-uridylic acid
  • 2'-Deoxyuridine 5'-monophosphate
  • 2'-Deoxyuridine 5'-phosphate
  • 2'-Deoxyuridylate
964-26-1
genes like me logo Genes that share compounds with DUT: view

Transcripts for DUT Gene

Unigene Clusters for DUT Gene

Deoxyuridine triphosphatase:
Representative Sequences:

Inhibitory RNA Products

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for DUT Gene

ExUns: 1a · 1b ^ 2a · 2b · 2c · 2d · 2e · 2f · 2g ^ 3a · 3b ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b
SP1: - - - - - - - - - -
SP2: - - - - -
SP3: - - -
SP4: - -
SP5: - - - - - - - - -
SP6: - -
SP7: - - -
SP8: -
SP9: - -

Relevant External Links for DUT Gene

GeneLoc Exon Structure for
DUT
ECgene alternative splicing isoforms for
DUT

Expression for DUT Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for DUT Gene

Protein differential expression in normal tissues from HIPED for DUT Gene

This gene is overexpressed in Peripheral blood mononuclear cells (10.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for DUT Gene



Protein tissue co-expression partners for DUT Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of DUT Gene:

DUT

SOURCE GeneReport for Unigene cluster for DUT Gene:

Hs.527980

mRNA Expression by UniProt/SwissProt for DUT Gene:

P33316-DUT_HUMAN
Tissue specificity: Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level).

Evidence on tissue expression from TISSUES for DUT Gene

  • Nervous system(4.8)
  • Lung(4.6)
  • Liver(4.4)
  • Blood(4.3)
  • Eye(4.2)
  • Intestine(2.1)
genes like me logo Genes that share expression patterns with DUT: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for DUT Gene

Orthologs for DUT Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for DUT Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia DUT 33 34
  • 98.94 (n)
cow
(Bos Taurus)
Mammalia DUT 33 34
  • 89.41 (n)
-- 34
  • 82 (a)
OneToMany
dog
(Canis familiaris)
Mammalia DUT 33 34
  • 84.66 (n)
oppossum
(Monodelphis domestica)
Mammalia DUT 34
  • 83 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Dut 33 16 34
  • 81.53 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia -- 34
  • 81 (a)
OneToMany
-- 34
  • 81 (a)
OneToMany
rat
(Rattus norvegicus)
Mammalia Dut 33
  • 80.86 (n)
chicken
(Gallus gallus)
Aves DUT 33 34
  • 75.97 (n)
lizard
(Anolis carolinensis)
Reptilia DUT 34
  • 65 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia LOC101733437 33
  • 66.28 (n)
Str.1294 33
African clawed frog
(Xenopus laevis)
Amphibia Xl.7472 33
zebrafish
(Danio rerio)
Actinopterygii dut 33 34
  • 65.4 (n)
wufa28d11 33
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.2007 33
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP010159 33
  • 65.95 (n)
fruit fly
(Drosophila melanogaster)
Insecta dUTPase 33 34
  • 58.96 (n)
worm
(Caenorhabditis elegans)
Secernentea K07A1.2 35
  • 58 (a)
dut-1 33 34
  • 57.17 (n)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_AGR249C 33
  • 54.5 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes DUT1 34
  • 52 (a)
OneToOne
sea squirt
(Ciona savignyi)
Ascidiacea -- 34
  • 67 (a)
OneToMany
CSA.6436 34
  • 62 (a)
OneToMany
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.2142 33
Species where no ortholog for DUT was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for DUT Gene

ENSEMBL:
Gene Tree for DUT (if available)
TreeFam:
Gene Tree for DUT (if available)

Paralogs for DUT Gene

No data available for Paralogs for DUT Gene

Variants for DUT Gene

Sequence variations from dbSNP and Humsavar for DUT Gene

SNP ID Clin Chr 15 pos Variation AA Info Type
rs1000573130 -- 48,340,162(+) G/C 3_prime_UTR_variant, intron_variant
rs1000625389 -- 48,340,411(+) C/T 3_prime_UTR_variant, intron_variant
rs1000785048 -- 48,333,216(+) G/T intron_variant
rs1001102963 -- 48,340,836(+) C/T downstream_transcript_variant, genic_downstream_transcript_variant, intron_variant
rs1001133842 -- 48,332,947(+) A/T intron_variant

Structural Variations from Database of Genomic Variants (DGV) for DUT Gene

Variant ID Type Subtype PubMed ID
nsv1053885 CNV gain 25217958
esv3636413 CNV loss 21293372
esv3636412 CNV gain 21293372
esv3569565 CNV gain 25503493
esv2760030 CNV loss 17122850

Variation tolerance for DUT Gene

Residual Variation Intolerance Score: 42.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.69; 14.60% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for DUT Gene

Human Gene Mutation Database (HGMD)
DUT
SNPedia medical, phenotypic, and genealogical associations of SNPs for
DUT

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for DUT Gene

Disorders for DUT Gene

MalaCards: The human disease database

(1) MalaCards diseases for DUT Gene - From: HGMD and GeneCards

Disorder Aliases PubMed IDs
cysticercosis
  • taeniasis
- elite association - COSMIC cancer census association via MalaCards
Search DUT in MalaCards View complete list of genes associated with diseases

Additional Disease Information for DUT

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
genes like me logo Genes that share disorders with DUT: view

No data available for UniProtKB/Swiss-Prot and Genatlas for DUT Gene

Publications for DUT Gene

  1. Assignment of the human dUTPase gene (DUT) to chromosome 15q15-q21. 1 by fluorescence in situ hybridization. (PMID: 9070952) Cohen D … Pearlman RE (Genomics 1997) 2 3 4 22 58
  2. Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme. (PMID: 1325640) McIntosh EM … Haynes RH (Proceedings of the National Academy of Sciences of the United States of America 1992) 2 3 4 22 58
  3. Active site closure facilitates juxtaposition of reactant atoms for initiation of catalysis by human dUTPase. (PMID: 17880943) Varga B … Vértessy BG (FEBS letters 2007) 3 4 22 58
  4. The human dUTPase gene encodes both nuclear and mitochondrial isoforms. Differential expression of the isoforms and characterization of a cDNA encoding the mitochondrial species. (PMID: 9228092) Ladner RD … Caradonna SJ (The Journal of biological chemistry 1997) 3 4 22 58
  5. Human dUTP pyrophosphatase: uracil recognition by a beta hairpin and active sites formed by three separate subunits. (PMID: 8805593) Mol CD … Tainer JA (Structure (London, England : 1993) 1996) 3 4 22 58

Products for DUT Gene

Sources for DUT Gene

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