DTX3 functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]).[supplied by OMIM, Nov 2009] See more...

Aliases for DTX3 Gene

Aliases for DTX3 Gene

  • Deltex E3 Ubiquitin Ligase 3 2 3 5
  • RNF154 2 3 4
  • Probable E3 Ubiquitin-Protein Ligase DTX3 3 4
  • RING-Type E3 Ubiquitin Transferase DTX3 3 4
  • RING Finger Protein 154 3 4
  • Protein Deltex-3 3 4
  • Deltex3 3 4
  • Deltex 3 Homolog (Drosophila) 2
  • Deltex Homolog 3 (Drosophila) 2
  • Deltex 3, E3 Ubiquitin Ligase 3
  • Deltex Homolog 3 3
  • EC 2.3.2.27 4
  • FLJ34766 2
  • DTX3 5

External Ids for DTX3 Gene

Previous GeneCards Identifiers for DTX3 Gene

  • GC12P056284
  • GC12P057998
  • GC12P055036

Summaries for DTX3 Gene

Entrez Gene Summary for DTX3 Gene

  • DTX3 functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]).[supplied by OMIM, Nov 2009]

GeneCards Summary for DTX3 Gene

DTX3 (Deltex E3 Ubiquitin Ligase 3) is a Protein Coding gene. Among its related pathways are Notch Signaling Pathway (sino) and Notch Signaling Pathway (WikiPathways). Gene Ontology (GO) annotations related to this gene include ligase activity. An important paralog of this gene is DTX3L.

UniProtKB/Swiss-Prot Summary for DTX3 Gene

  • Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context (By similarity). Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for DTX3 Gene

Genomics for DTX3 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for DTX3 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around DTX3 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for DTX3

Top Transcription factor binding sites by QIAGEN in the DTX3 gene promoter:
  • AP-4
  • E47
  • IRF-1
  • ITF-2
  • NF-kappaB
  • NF-kappaB1
  • Pax-3
  • RORalpha1
  • Tal-1
  • Tal-1beta

Genomic Locations for DTX3 Gene

Genomic Locations for DTX3 Gene
chr12:57,604,327-57,609,804
(GRCh38/hg38)
Size:
5,478 bases
Orientation:
Plus strand
chr12:57,998,405-58,003,587
(GRCh37/hg19)
Size:
5,183 bases
Orientation:
Plus strand

Genomic View for DTX3 Gene

Genes around DTX3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
DTX3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for DTX3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for DTX3 Gene

Proteins for DTX3 Gene

  • Protein details for DTX3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8N9I9-DTX3_HUMAN
    Recommended name:
    Probable E3 ubiquitin-protein ligase DTX3
    Protein Accession:
    Q8N9I9
    Secondary Accessions:
    • Q53ZZ2
    • Q8NAU6
    • Q8NDS8

    Protein attributes for DTX3 Gene

    Size:
    347 amino acids
    Molecular mass:
    37988 Da
    Quaternary structure:
    • Homodimer. May form a heterodimers with other members of the Deltex family. Interacts with NOTCH1.
    SequenceCaution:
    • Sequence=CAD38593.2; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};

    Alternative splice isoforms for DTX3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for DTX3 Gene

Post-translational modifications for DTX3 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for DTX3 Gene

Domains & Families for DTX3 Gene

Gene Families for DTX3 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins

Protein Domains for DTX3 Gene

InterPro:
Blocks:
  • Zn-finger, RING
ProtoNet:

Suggested Antigen Peptide Sequences for DTX3 Gene

GenScript: Design optimal peptide antigens:
  • RING finger protein 154 (DTX3_HUMAN)
  • cDNA FLJ44795 fis, clone BRACE3040239, highly similar to Protein deltex-3 (Q53ZZ2_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q8N9I9

UniProtKB/Swiss-Prot:

DTX3_HUMAN :
  • Belongs to the Deltex family.
Family:
  • Belongs to the Deltex family.
genes like me logo Genes that share domains with DTX3: view

Function for DTX3 Gene

Molecular function for DTX3 Gene

UniProtKB/Swiss-Prot Function:
Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context (By similarity). Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; Evidence={ECO:0000250|UniProtKB:Q61010};.

Enzyme Numbers (IUBMB) for DTX3 Gene

Phenotypes From GWAS Catalog for DTX3 Gene

Gene Ontology (GO) - Molecular Function for DTX3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 19549727
GO:0016740 transferase activity IEA --
GO:0046872 metal ion binding IEA --
GO:0061630 ubiquitin protein ligase activity IBA 21873635
genes like me logo Genes that share ontologies with DTX3: view
genes like me logo Genes that share phenotypes with DTX3: view

Animal Model Products

CRISPR Products

miRNA for DTX3 Gene

miRTarBase miRNAs that target DTX3

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for DTX3

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for DTX3 Gene

Localization for DTX3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for DTX3 Gene

Cytoplasm.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for DTX3 gene
Compartment Confidence
nucleus 4
cytosol 2
golgi apparatus 2
plasma membrane 1
extracellular 1
cytoskeleton 1
mitochondrion 1
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Golgi apparatus (2)
  • Nucleoli (2)
  • Nucleoplasm (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for DTX3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005654 nucleoplasm IBA 21873635
GO:0005737 cytoplasm IEA --
genes like me logo Genes that share ontologies with DTX3: view

Pathways & Interactions for DTX3 Gene

genes like me logo Genes that share pathways with DTX3: view

Pathways by source for DTX3 Gene

1 Sino Biological pathway for DTX3 Gene
1 BioSystems pathway for DTX3 Gene
1 KEGG pathway for DTX3 Gene
1 GeneTex pathway for DTX3 Gene

UniProtKB/Swiss-Prot Q8N9I9-DTX3_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

Gene Ontology (GO) - Biological Process for DTX3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007219 Notch signaling pathway IEA,IBA 21873635
GO:0016567 protein ubiquitination IEA --
genes like me logo Genes that share ontologies with DTX3: view

No data available for SIGNOR curated interactions for DTX3 Gene

Drugs & Compounds for DTX3 Gene

No Compound Related Data Available

Transcripts for DTX3 Gene

mRNA/cDNA for DTX3 Gene

3 REFSEQ mRNAs :
10 NCBI additional mRNA sequence :
9 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for DTX3

Alternative Splicing Database (ASD) splice patterns (SP) for DTX3 Gene

ExUns: 1a · 1b ^ 2a · 2b ^ 3a · 3b ^ 4 ^ 5a · 5b ^ 6a · 6b · 6c · 6d · 6e · 6f · 6g ^ 7a · 7b ^ 8 ^ 9
SP1: - -
SP2: - - -
SP3: - - - -
SP4: - - - - -
SP5: - -
SP6: - - -
SP7: - -
SP8: - -
SP9: -
SP10:
SP11:
SP12:

Relevant External Links for DTX3 Gene

GeneLoc Exon Structure for
DTX3

Expression for DTX3 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for DTX3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for DTX3 Gene

This gene is overexpressed in Retina (12.8), Rectum (11.2), Fetal Brain (8.6), and Stomach (8.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for DTX3 Gene



Protein tissue co-expression partners for DTX3 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for DTX3

SOURCE GeneReport for Unigene cluster for DTX3 Gene:

Hs.32374

Evidence on tissue expression from TISSUES for DTX3 Gene

  • Nervous system(4.8)
  • Pancreas(2.3)
genes like me logo Genes that share expression patterns with DTX3: view

No data available for mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for DTX3 Gene

Orthologs for DTX3 Gene

This gene was present in the common ancestor of animals.

Orthologs for DTX3 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia DTX3 30 31
  • 97.08 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia DTX3 30 31
  • 92.32 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia DTX3 30 31
  • 91.24 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Dtx3 30
  • 90.97 (n)
Mouse
(Mus musculus)
Mammalia Dtx3 30 17 31
  • 90.11 (n)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia DTX3 31
  • 80 (a)
OneToOne
Chicken
(Gallus gallus)
Aves -- 31
  • 24 (a)
ManyToMany
Lizard
(Anolis carolinensis)
Reptilia DTX3 31
  • 76 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia dtx3 30
  • 68.43 (n)
Str.17194 30
Zebrafish
(Danio rerio)
Actinopterygii zgc:66440 30
  • 59.18 (n)
DTX3 31
  • 42 (a)
OneToOne
zgc66440 30
Fruit Fly
(Drosophila melanogaster)
Insecta dx 31
  • 13 (a)
OneToMany
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 48 (a)
OneToOne
Species where no ortholog for DTX3 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Oppossum (Monodelphis domestica)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for DTX3 Gene

ENSEMBL:
Gene Tree for DTX3 (if available)
TreeFam:
Gene Tree for DTX3 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for DTX3: view image

Paralogs for DTX3 Gene

Paralogs for DTX3 Gene

(3) SIMAP similar genes for DTX3 Gene using alignment to 4 proteins:

  • DTX3_HUMAN
  • F8VUZ4_HUMAN
  • F8VV96_HUMAN
  • H0YHF9_HUMAN
genes like me logo Genes that share paralogs with DTX3: view

Variants for DTX3 Gene

Additional dbSNP identifiers (rs#s) for DTX3 Gene

Structural Variations from Database of Genomic Variants (DGV) for DTX3 Gene

Variant ID Type Subtype PubMed ID
esv2759901 CNV gain 17122850
nsv428283 CNV gain 18775914
nsv482992 CNV gain+loss 15286789
nsv559034 CNV loss 21841781

Variation tolerance for DTX3 Gene

Residual Variation Intolerance Score: 30.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.44; 9.59% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for DTX3 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
DTX3

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for DTX3 Gene

Disorders for DTX3 Gene

No disorders were found for DTX3 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for DTX3 Gene

Publications for DTX3 Gene

  1. The BAL-binding protein BBAP and related Deltex family members exhibit ubiquitin-protein isopeptide ligase activity. (PMID: 12670957) Takeyama K … Shipp MA (The Journal of biological chemistry 2003) 2 3 4 23
  2. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4
  3. A reference map of the human binary protein interactome. (PMID: 32296183) Luck K … Calderwood MA (Nature 2020) 3
  4. Ubiquitination of NOTCH2 by DTX3 suppresses the proliferation and migration of human esophageal carcinoma. (PMID: 31854042) Ding XY … Qin X (Cancer science 2020) 3
  5. Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity. (PMID: 29643511) Pao KC … Virdee S (Nature 2018) 3

Products for DTX3 Gene

  • Signalway Proteins for DTX3

Sources for DTX3 Gene