Transcriptional repression is a general mechanism for regulating transcriptional initiation in organisms ranging from yeast to humans. Accurate initiation of transcription from eukaryotic protein-encoding genes requires the assembly of a large multiprotein complex consisting of RNA polymerase II and general transcription factors such as TFIIA, TFIIB, and TFIID. DR1 is a repres... See more...

Aliases for DRAP1 Gene

Aliases for DRAP1 Gene

  • DR1 Associated Protein 1 2 3 5
  • NC2-Alpha 2 3 4
  • Dr1-Associated Corepressor 3 4
  • Negative Cofactor 2 Alpha 2 3
  • DR1-Associated Corepressor 2
  • Negative Co-Factor 2-Alpha 3
  • Negative Cofactor 2-Alpha 4
  • Dr1-Associated Protein 1 4
  • DRAP1 5

External Ids for DRAP1 Gene

Previous GeneCards Identifiers for DRAP1 Gene

  • GC11P068221
  • GC11P067368
  • GC11P065936
  • GC11P065462
  • GC11P065443
  • GC11P065686
  • GC11P062013
  • GC11P065924
  • GC11P065925
  • GC11P065926

Summaries for DRAP1 Gene

Entrez Gene Summary for DRAP1 Gene

  • Transcriptional repression is a general mechanism for regulating transcriptional initiation in organisms ranging from yeast to humans. Accurate initiation of transcription from eukaryotic protein-encoding genes requires the assembly of a large multiprotein complex consisting of RNA polymerase II and general transcription factors such as TFIIA, TFIIB, and TFIID. DR1 is a repressor that interacts with the TATA-binding protein (TBP) of TFIID and prevents the formation of an active transcription complex by precluding the entry of TFIIA and/or TFIIB into the preinitiation complex. The protein encoded by this gene is a corepressor of transcription that interacts with DR1 to enhance DR1-mediated repression. The interaction between this corepressor and DR1 is required for corepressor function and appears to stabilize the TBP-DR1-DNA complex. [provided by RefSeq, Jul 2008]

GeneCards Summary for DRAP1 Gene

DRAP1 (DR1 Associated Protein 1) is a Protein Coding gene. Among its related pathways are Signaling by GPCR and Signaling by NODAL. Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and protein heterodimerization activity. An important paralog of this gene is NFYC.

UniProtKB/Swiss-Prot Summary for DRAP1 Gene

  • The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own.

Gene Wiki entry for DRAP1 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for DRAP1 Gene

Genomics for DRAP1 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for DRAP1 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around DRAP1 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for DRAP1

Top Transcription factor binding sites by QIAGEN in the DRAP1 gene promoter:
  • ATF-2
  • C/EBPalpha
  • CRE-BP1
  • p300
  • p53
  • STAT1
  • TBP

Genomic Locations for DRAP1 Gene

Latest Assembly
chr11:65,919,274-65,921,563
(GRCh38/hg38)
Size:
2,290 bases
Orientation:
Plus strand

Previous Assembly
chr11:65,686,897-65,689,034
(GRCh37/hg19 by Entrez Gene)
Size:
2,138 bases
Orientation:
Plus strand

chr11:65,686,728-65,689,032
(GRCh37/hg19 by Ensembl)
Size:
2,305 bases
Orientation:
Plus strand

Genomic View for DRAP1 Gene

Genes around DRAP1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
DRAP1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for DRAP1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for DRAP1 Gene

Proteins for DRAP1 Gene

  • Protein details for DRAP1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q14919-NC2A_HUMAN
    Recommended name:
    Dr1-associated corepressor
    Protein Accession:
    Q14919
    Secondary Accessions:
    • Q13448

    Protein attributes for DRAP1 Gene

    Size:
    205 amino acids
    Molecular mass:
    22350 Da
    Quaternary structure:
    • Heterodimer with DR1. Binds BTAF1.

    Three dimensional structures from OCA and Proteopedia for DRAP1 Gene

    Alternative splice isoforms for DRAP1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for DRAP1 Gene

Post-translational modifications for DRAP1 Gene

  • Phosphorylation reduces DNA binding, but has no effect on heterodimerization and TBP binding.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for DRAP1 Gene

Antibodies for research

No data available for DME Specific Peptides for DRAP1 Gene

Domains & Families for DRAP1 Gene

Gene Families for DRAP1 Gene

Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for DRAP1 Gene

InterPro:
Blocks:
  • Transcription factor CBF/NF-Y/archaeal histone
ProtoNet:

Suggested Antigen Peptide Sequences for DRAP1 Gene

GenScript: Design optimal peptide antigens:
  • Negative co-factor 2-alpha (NC2A_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q14919

UniProtKB/Swiss-Prot:

NC2A_HUMAN :
  • Belongs to the NC2 alpha/DRAP1 family.
Family:
  • Belongs to the NC2 alpha/DRAP1 family.
genes like me logo Genes that share domains with DRAP1: view

Function for DRAP1 Gene

Molecular function for DRAP1 Gene

UniProtKB/Swiss-Prot Function:
The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own.
GENATLAS Biochemistry:
Dr1-associated polypeptide,corepressor of transcription,expressed with higher levels in tissues with low mitotic index

Phenotypes From GWAS Catalog for DRAP1 Gene

Gene Ontology (GO) - Molecular Function for DRAP1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001046 core promoter sequence-specific DNA binding IBA 21873635
GO:0001091 RNA polymerase II basal transcription factor binding IPI 8670811
GO:0003677 contributes_to DNA binding IDA 8670811
GO:0003713 transcription coactivator activity IBA 21873635
GO:0003714 transcription corepressor activity IBA 21873635
genes like me logo Genes that share ontologies with DRAP1: view
genes like me logo Genes that share phenotypes with DRAP1: view

Animal Models for DRAP1 Gene

MGI Knock Outs for DRAP1:

miRNA for DRAP1 Gene

miRTarBase miRNAs that target DRAP1

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for DRAP1

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for DRAP1 Gene

Localization for DRAP1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for DRAP1 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for DRAP1 gene
Compartment Confidence
nucleus 4
cytosol 3
plasma membrane 1
extracellular 1
cytoskeleton 1
mitochondrion 1
peroxisome 0
endoplasmic reticulum 0

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for DRAP1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IBA 21873635
GO:0017054 negative cofactor 2 complex IBA 21873635
GO:0090575 RNA polymerase II transcription factor complex IDA 8670811
genes like me logo Genes that share ontologies with DRAP1: view

Pathways & Interactions for DRAP1 Gene

PathCards logo

SuperPathways for DRAP1 Gene

genes like me logo Genes that share pathways with DRAP1: view

Pathways by source for DRAP1 Gene

Gene Ontology (GO) - Biological Process for DRAP1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000122 negative regulation of transcription by RNA polymerase II TAS,IDA 8670811
GO:0006355 regulation of transcription, DNA-templated IBA 21873635
GO:0006366 transcription by RNA polymerase II IBA 21873635
GO:0045944 positive regulation of transcription by RNA polymerase II IBA 21873635
genes like me logo Genes that share ontologies with DRAP1: view

No data available for SIGNOR curated interactions for DRAP1 Gene

Drugs & Compounds for DRAP1 Gene

No Compound Related Data Available

Transcripts for DRAP1 Gene

mRNA/cDNA for DRAP1 Gene

1 REFSEQ mRNAs :
5 NCBI additional mRNA sequence :
9 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for DRAP1

Alternative Splicing Database (ASD) splice patterns (SP) for DRAP1 Gene

ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b · 6c ^ 7a · 7b
SP1: - -
SP2: -
SP3: - -
SP4: - -
SP5:

Relevant External Links for DRAP1 Gene

GeneLoc Exon Structure for
DRAP1

Expression for DRAP1 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for DRAP1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for DRAP1 Gene

This gene is overexpressed in CD8 Tcells (14.1), Blymphocyte (7.3), and Peripheral blood mononuclear cells (7.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for DRAP1 Gene



Protein tissue co-expression partners for DRAP1 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for DRAP1

SOURCE GeneReport for Unigene cluster for DRAP1 Gene:

Hs.356742

mRNA Expression by UniProt/SwissProt for DRAP1 Gene:

Q14919-NC2A_HUMAN
Tissue specificity: Ubiquitous. Highly expressed in adult testis, heart, skeletal muscle, pancreas and brain, and in fetal brain, liver and kidney.

Evidence on tissue expression from TISSUES for DRAP1 Gene

  • Muscle(4.5)
  • Nervous system(4.2)
  • Lung(4)
  • Skin(3.9)
  • Pancreas(3.9)
  • Eye(3.3)
  • Intestine(3.3)
  • Bone(2.9)
  • Liver(2.4)
  • Kidney(2.2)
genes like me logo Genes that share expression patterns with DRAP1: view

Primer products for research

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for DRAP1 Gene

Orthologs for DRAP1 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for DRAP1 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia DRAP1 30 31
  • 97.62 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia -- 31
  • 94 (a)
OneToMany
DRAP1 30
  • 89.27 (n)
-- 31
  • 65 (a)
OneToMany
Cow
(Bos Taurus)
Mammalia DRAP1 30 31
  • 91.54 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Drap1 30
  • 88.77 (n)
Mouse
(Mus musculus)
Mammalia Drap1 30 17 31
  • 88.58 (n)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia DRAP1 31
  • 52 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia drap1 30
  • 69.84 (n)
MGC75665 30
African clawed frog
(Xenopus laevis)
Amphibia Xl.23737 30
Zebrafish
(Danio rerio)
Actinopterygii drap1 30 31
  • 76.73 (n)
OneToOne
Dr.719 30
Fruit Fly
(Drosophila melanogaster)
Insecta NC2alpha 31 32
  • 27 (a)
OneToOne
Worm
(Caenorhabditis elegans)
Secernentea drap-1 31
  • 14 (a)
OneToOne
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes BUR6 31 33
  • 27 (a)
OneToOne
Sea Squirt
(Ciona savignyi)
Ascidiacea CSA.9522 31
  • 30 (a)
OneToOne
Sea Vase
(Ciona intestinalis)
Ascidiacea Cin.259 30
Species where no ortholog for DRAP1 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Lizard (Anolis carolinensis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Oppossum (Monodelphis domestica)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for DRAP1 Gene

ENSEMBL:
Gene Tree for DRAP1 (if available)
TreeFam:
Gene Tree for DRAP1 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for DRAP1: view image

Paralogs for DRAP1 Gene

Paralogs for DRAP1 Gene

genes like me logo Genes that share paralogs with DRAP1: view

Variants for DRAP1 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for DRAP1 Gene

SNP ID Clinical significance and condition Chr 11 pos Variation AA Info Type
rs149754486 Benign: not provided 65,919,785(+) G/A
NM_006442.4(DRAP1):c.48G>A (p.Arg16=)
SYNONYMOUS

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for DRAP1 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for DRAP1 Gene

Variant ID Type Subtype PubMed ID
esv2759834 CNV gain 17122850
nsv1159905 CNV duplication 26073780
nsv832191 CNV loss 17160897

Variation tolerance for DRAP1 Gene

Residual Variation Intolerance Score: 49.3% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.99; 20.37% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for DRAP1 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
DRAP1

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for DRAP1 Gene

Disorders for DRAP1 Gene

Additional Disease Information for DRAP1

No disorders were found for DRAP1 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for DRAP1 Gene

Publications for DRAP1 Gene

  1. NC2alpha interacts with BTAF1 and stimulates its ATP-dependent association with TATA-binding protein. (PMID: 15509807) Klejman MP … Timmers HT (Molecular and cellular biology 2004) 3 4 23
  2. Requirement of a corepressor for Dr1-mediated repression of transcription. (PMID: 8608938) Mermelstein F … Reinberg D (Genes & development 1996) 2 3 4
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4
  4. Crystal structure of negative cofactor 2 recognizing the TBP-DNA transcription complex. (PMID: 11461703) Kamada K … Burley SK (Cell 2001) 3 4
  5. A mechanism for repression of class II gene transcription through specific binding of NC2 to TBP-promoter complexes via heterodimeric histone fold domains. (PMID: 8670811) Goppelt A … Meisterernst M (The EMBO journal 1996) 3 4

Products for DRAP1 Gene

Sources for DRAP1 Gene