DPF2 Gene (Protein Coding)

Double PHD Fingers 2

GC11P065351
GIFtS:
42
The protein encoded by this gene is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidat... See more...

Aliases for DPF2 Gene

Aliases for DPF2 Gene

  • Double PHD Fingers 2 2 3 5
  • D4, Zinc And Double PHD Fingers Family 2 2 3 4
  • Apoptosis Response Zinc Finger Protein 3 4
  • Zinc Finger Protein Ubi-D4 3 4
  • BRG1-Associated Factor 45D 3 4
  • Protein Requiem 3 4
  • Ubi-D4 2 3
  • BAF45D 3 4
  • UBID4 3 4
  • REQ 3 4
  • Requiem, Apoptosis Response Zinc Finger Gene 2
  • Requiem, Apoptosis Response Zinc Finger 3
  • BAF45d 2
  • CSS7 3
  • DPF2 5

External Ids for DPF2 Gene

Previous HGNC Symbols for DPF2 Gene

  • REQ

Previous GeneCards Identifiers for DPF2 Gene

  • GC11P064876
  • GC11P064858
  • GC11P065103
  • GC11P061428

Summaries for DPF2 Gene

Entrez Gene Summary for DPF2 Gene

  • The protein encoded by this gene is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors. [provided by RefSeq, Jul 2008]

GeneCards Summary for DPF2 Gene

DPF2 (Double PHD Fingers 2) is a Protein Coding gene. Diseases associated with DPF2 include Coffin-Siris Syndrome 7 and Coffin-Siris Syndrome 1. Gene Ontology (GO) annotations related to this gene include RNA polymerase II proximal promoter sequence-specific DNA binding. An important paralog of this gene is DPF1.

UniProtKB/Swiss-Prot Summary for DPF2 Gene

  • Plays an active role in transcriptional regulation by binding modified histones H3 and H4 (PubMed:28533407, PubMed:27775714). Is a negative regulator of myeloid differentiation of hematopoietic progenitor cells (PubMed:28533407). Might also have a role in the development and maturation of lymphoid cells (By similarity). Involved in the regulation of non-canonical NF-kappa-B pathway (PubMed:20460684).

Gene Wiki entry for DPF2 Gene

Additional gene information for DPF2 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for DPF2 Gene

Genomics for DPF2 Gene

GeneHancer (GH) Regulatory Elements Pubs

Download GeneHancer 2017 data
Promoters and enhancers for DPF2 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around DPF2 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for DPF2

Top Transcription factor binding sites by QIAGEN in the DPF2 gene promoter:
  • AML1a
  • Elk-1
  • IRF-2
  • LCR-F1
  • NRF-2
  • SEF-1 (1)
  • Sox5
  • Sp1
  • STAT5A

Genomic Locations for DPF2 Gene

Latest Assembly
chr11:65,333,840-65,354,262
(GRCh38/hg38)
Size:
20,423 bases
Orientation:
Plus strand

Previous Assembly
chr11:65,101,323-65,121,733
(GRCh37/hg19 by Entrez Gene)
Size:
20,411 bases
Orientation:
Plus strand

chr11:65,101,225-65,120,720
(GRCh37/hg19 by Ensembl)
Size:
19,496 bases
Orientation:
Plus strand

Genomic View for DPF2 Gene

Genes around DPF2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
DPF2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for DPF2 Gene
GeneLoc Logo Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for DPF2 Gene

Proteins for DPF2 Gene

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  • Protein details for DPF2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q92785-REQU_HUMAN
    Recommended name:
    Zinc finger protein ubi-d4
    Protein Accession:
    Q92785
    Secondary Accessions:
    • A8K7C9
    • B4DT58

    Protein attributes for DPF2 Gene

    Size:
    391 amino acids
    Molecular mass:
    44155 Da
    Quaternary structure:
    • Interacts with the nucleosomes, in particular nucleosomes bearing histone H3 crotonylated at 'Lys-14' (H3K14cr) for which DPF2 has high affinity (PubMed:27775714). Also interacts (via PHD-type zinc finger domains) with histone H3 butyrylated at 'Lys-14' (H3K14bu), histone H3 propionylated at 'Lys-14' (H3K14pr), and histone H3 acetylated at 'Lys-14' (H3K14ac) (PubMed:29429572, PubMed:28533407, PubMed:27775714). Interacts with histone H3 acetylated at 'Lys-9' (H3K9ac), histone H3 di-methylated at 'Lys-9' (H3K9me2), and histone H3 tri-methylated at 'Lys-9' (H3K9me3) (PubMed:29429572). Interacts with histone H4 acetylated at 'Lys-12' (H4K12ac) (PubMed:29429572). Interacts with histone H4 acetylated at 'Lys-16' (H4K16ac) (PubMed:28533407). Interacts with SWI/SNF complex components (PubMed:20460684, PubMed:28533407). Interacts with SMARCA2, SMARCA4, SMARCB1 and SMARCD1 (PubMed:20460684). Interacts with SMARCC1, SMARCC2 and ACTL6A (PubMed:28533407). Interacts with RUNX1 (PubMed:28533407).

    Three dimensional structures from OCA and Proteopedia for DPF2 Gene

    Alternative splice isoforms for DPF2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for DPF2 Gene

Protein Expression for DPF2 Gene

See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB

Post-translational modifications for DPF2 Gene

  • Ubiquitination at Lys59 and Lys108
  • Modification sites at PhosphoSitePlus

Other Protein References for DPF2 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for DPF2 Gene

Domains & Families for DPF2 Gene

Gene Families for DPF2 Gene

HGNC:
Human Protein Atlas (HPA):
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for DPF2 Gene

InterPro:
Blocks:
  • Zn-finger, RING
  • Zn-finger-like, PHD finger
ProtoNet:

Suggested Antigen Peptide Sequences for DPF2 Gene

GenScript: Design optimal peptide antigens:
  • cDNA FLJ54147, highly similar to Zinc-finger protein ubi-d4 (B4DT58_HUMAN)
  • Protein requiem (REQU_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q92785

UniProtKB/Swiss-Prot:

REQU_HUMAN :
  • Belongs to the requiem/DPF family.
Family:
  • Belongs to the requiem/DPF family.
genes like me logo Genes that share domains with DPF2: view

Function for DPF2 Gene

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  6. Cell Lines for research

Molecular function for DPF2 Gene

UniProtKB/Swiss-Prot Function:
Plays an active role in transcriptional regulation by binding modified histones H3 and H4 (PubMed:28533407, PubMed:27775714). Is a negative regulator of myeloid differentiation of hematopoietic progenitor cells (PubMed:28533407). Might also have a role in the development and maturation of lymphoid cells (By similarity). Involved in the regulation of non-canonical NF-kappa-B pathway (PubMed:20460684).

Phenotypes From GWAS Catalog for DPF2 Gene

Gene Ontology (GO) - Molecular Function for DPF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003712 transcription coregulator activity IBA 21873635
GO:0003714 transcription corepressor activity TAS 28533407
GO:0005515 protein binding IPI 25416956
GO:0042393 histone binding IBA 21873635
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with DPF2: view
genes like me logo Genes that share phenotypes with DPF2: view

Human Phenotype Ontology for DPF2 Gene

HPO Id HPO Name Alternative Ids Definition Synonyms

Animal Models for DPF2 Gene

MGI Knock Outs for DPF2:
  • Dpf2 Dpf2<tm1b(EUCOMM)Hmgu>

Animal Models for research

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for DPF2

No data available for Enzyme Numbers (IUBMB) , Transcription Factor Targets and HOMER Transcription for DPF2 Gene

Localization for DPF2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for DPF2 Gene

Nucleus. Cytoplasm.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for DPF2 gene
Compartment Confidence
nucleus 5
cytosol 5
cytoskeleton 4
extracellular 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nucleoplasm (3)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for DPF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000785 chromatin IDA 28533407
GO:0005634 nucleus IEA,IDA 29429572
GO:0005654 nucleoplasm IDA --
GO:0005737 cytoplasm IEA --
GO:0005813 centrosome IDA --
genes like me logo Genes that share ontologies with DPF2: view

Pathways & Interactions for DPF2 Gene

PathCards logo

SuperPathways for DPF2 Gene

No Data Available

SIGNOR curated interactions for DPF2 Gene

Inactivates:

Gene Ontology (GO) - Biological Process for DPF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000122 negative regulation of transcription by RNA polymerase II TAS 28533407
GO:0006325 chromatin organization IEA --
GO:0006915 apoptotic process TAS 8812431
GO:0007399 nervous system development IBA 21873635
GO:0045892 negative regulation of transcription, DNA-templated IBA 21873635
genes like me logo Genes that share ontologies with DPF2: view

No data available for Pathways by source for DPF2 Gene

Drugs & Compounds for DPF2 Gene

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No Compound Related Data Available

Transcripts for DPF2 Gene

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mRNA/cDNA for DPF2 Gene

2 REFSEQ mRNAs :
15 NCBI additional mRNA sequence :
12 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for DPF2

Alternative Splicing Database (ASD) splice patterns (SP) for DPF2 Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5a · 5b · 5c ^ 6a · 6b ^ 7a · 7b · 7c ^ 8 ^ 9a · 9b ^ 10 ^ 11 ^ 12
SP1: - -
SP2: - - -
SP3: -
SP4: - - - -
SP5:

Relevant External Links for DPF2 Gene

GeneLoc Exon Structure for
DPF2

Expression for DPF2 Gene

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mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for DPF2 Gene

mRNA expression in normal human tissues for DPF2 Gene
mRNA expression by GTEx for DPF2 GenemRNA expression by BioGPS for DPF2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for DPF2 Gene

This gene is overexpressed in Peripheral blood mononuclear cells (24.5).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for DPF2 Gene



Protein tissue co-expression partners for DPF2 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for DPF2

SOURCE GeneReport for Unigene cluster for DPF2 Gene:

Hs.13495

mRNA Expression by UniProt/SwissProt for DPF2 Gene:

Q92785-REQU_HUMAN
Tissue specificity: Ubiquitous.

Evidence on tissue expression from TISSUES for DPF2 Gene

  • Nervous system(4.5)
  • Liver(4.2)
  • Muscle(2.4)
  • Intestine(2)
genes like me logo Genes that share expression patterns with DPF2: view

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for DPF2 Gene

Orthologs for DPF2 Gene

This gene was present in the common ancestor of animals.

Orthologs for DPF2 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
 Mammalia DPF2 30 31
  • 99.57 (n)
 OneToOne
Cow
(Bos Taurus)
 Mammalia DPF2 30 31
  • 93.86 (n)
 OneToOne
Oppossum
(Monodelphis domestica)
 Mammalia DPF2 31
  • 93 (a)
 OneToOne
Dog
(Canis familiaris)
 Mammalia DPF2 30 31
  • 92.5 (n)
 OneToOne
Rat
(Rattus norvegicus)
 Mammalia Dpf2 30
  • 91.3 (n)
Mouse
(Mus musculus)
 Mammalia Dpf2 30 17 31
  • 90.54 (n)
 OneToOne
Platypus
(Ornithorhynchus anatinus)
 Mammalia DPF2 31
  • 88 (a)
 OneToOne
Chicken
(Gallus gallus)
 Aves DPF2 30 31
  • 82.1 (n)
 OneToOne
Lizard
(Anolis carolinensis)
 Reptilia -- 31
  • 96 (a)
 OneToMany
-- 31
  • 87 (a)
 OneToMany
Tropical Clawed Frog
(Silurana tropicalis)
 Amphibia dpf2 30
  • 76.28 (n)
Str.10851 30
African clawed frog
(Xenopus laevis)
 Amphibia req-A 30
Zebrafish
(Danio rerio)
 Actinopterygii dpf2l 31
  • 74 (a)
 OneToMany
dpf2 30 31
  • 72.15 (n)
 OneToMany
Fruit Fly
(Drosophila melanogaster)
 Insecta d4 31
  • 32 (a)
 ManyToMany
tth 31
  • 15 (a)
 ManyToMany
Worm
(Caenorhabditis elegans)
 Secernentea dpff-1 31
  • 33 (a)
 OneToMany
Sea Squirt
(Ciona savignyi)
 Ascidiacea -- 31
  • 44 (a)
 OneToMany
Sea Vase
(Ciona intestinalis)
 Ascidiacea Cin.12010 30
Species where no ortholog for DPF2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for DPF2 Gene

ENSEMBL:
Gene Tree for DPF2 (if available)
TreeFam:
Gene Tree for DPF2 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for DPF2: view image

Paralogs for DPF2 Gene

Paralogs for DPF2 Gene

(2) SIMAP similar genes for DPF2 Gene using alignment to 5 proteins:

  • REQU_HUMAN
  • B4DT58_HUMAN
  • E9PN04_HUMAN
  • H0YEI1_HUMAN
  • J3KMZ8_HUMAN

Pseudogenes.org Pseudogenes for DPF2 Gene

genes like me logo Genes that share paralogs with DPF2: view

Variants for DPF2 Gene

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Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for DPF2 Gene

SNP ID Clinical significance and condition Chr 11 pos Variation AA Info Type
930733 Uncertain Significance: Coffin-Siris syndrome 7 65,341,461(+) C/G
NM_006268.5(DPF2):c.364C>G (p.Leu122Val) 		MISSENSE
985019 Pathogenic: Inborn genetic diseases 65,346,059(+) G/A
NM_006268.5(DPF2):c.904+1G>A 		SPLICE_DONOR
985278 Uncertain Significance: Inborn genetic diseases 65,345,975(+) A/C
NM_006268.5(DPF2):c.821A>C (p.Asp274Ala) 		MISSENSE
985546 Uncertain Significance: Inborn genetic diseases 65,344,033(+) A/C
NM_006268.5(DPF2):c.601A>C (p.Ile201Leu) 		MISSENSE
985635 Uncertain Significance: Inborn genetic diseases 65,346,018(+) A/C
NM_006268.5(DPF2):c.864A>C (p.Gln288His) 		MISSENSE

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for DPF2 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for DPF2 Gene

Variant ID Type Subtype PubMed ID
esv2422285 CNV deletion 17116639
nsv526181 CNV gain 19592680
nsv825950 CNV gain 20364138
nsv832190 CNV loss 17160897

Variation tolerance for DPF2 Gene

Residual Variation Intolerance Score: 17.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.16; 3.63% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for DPF2 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
DPF2

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for DPF2 Gene

Disorders for DPF2 Gene

MalaCards: The human disease database

(8) MalaCards diseases for DPF2 Gene - From: OMI, CVR, GTR, ORP, SWI, COP, and GCD

Disorder Aliases PubMed IDs
coffin-siris syndrome 7
  • css7
coffin-siris syndrome 1
  • css1
multiple endocrine neoplasia
  • multiple endocrine adenomatosis
flinders island spotted fever
  • fisf
small-cell carcinoma of the ovary of hypercalcemic type
  • hypercalcemic type ovarian small cell carcinoma
- elite association - COSMIC cancer census association via MalaCards
Search DPF2 in MalaCards View complete list of genes associated with diseases

UniProtKB/Swiss-Prot

REQU_HUMAN
  • Coffin-Siris syndrome 7 (CSS7) [MIM:618027]: A form of Coffin-Siris syndrome, a congenital multiple malformation syndrome with broad phenotypic and genetic variability. Cardinal features are intellectual disability, coarse facial features, hypertrichosis, and hypoplastic or absent fifth digit nails or phalanges. Additional features include malformations of the cardiac, gastrointestinal, genitourinary, and/or central nervous systems. Sucking/feeding difficulties, poor growth, ophthalmologic abnormalities, hearing impairment, and spinal anomalies are common findings. Both autosomal dominant and autosomal recessive inheritance patterns have been reported. CSS7 inheritance is autosomal dominant. {ECO:0000269 PubMed:29429572}. Note=The disease is caused by variants affecting the gene represented in this entry.

Additional Disease Information for DPF2

Atlas
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
Open Targets Platform
genes like me logo Genes that share disorders with DPF2: view

No data available for Genatlas for DPF2 Gene

Publications for DPF2 Gene

  1. Mutations in the BAF-Complex Subunit DPF2 Are Associated with Coffin-Siris Syndrome. (PMID: 29429572) Vasileiou G … Reis A (American journal of human genetics 2018) 3 4 74
  2. Histone-binding of DPF2 mediates its repressive role in myeloid differentiation. (PMID: 28533407) Huber FM … Hoelz A (Proceedings of the National Academy of Sciences of the United States of America 2017) 3 4
  3. Selective recognition of histone crotonylation by double PHD fingers of MOZ and DPF2. (PMID: 27775714) Xiong X … Li H (Nature chemical biology 2016) 3 4
  4. Crystal structure of the Cys2His2-type zinc finger domain of human DPF2. (PMID: 21888896) Zhang W … Qi C (Biochemical and biophysical research communications 2011) 3 4
  5. Requiem protein links RelB/p52 and the Brm-type SWI/SNF complex in a noncanonical NF-kappaB pathway. (PMID: 20460684) Tando T … Iba H (The Journal of biological chemistry 2010) 3 4

ExternalLinks

View latest publications for DPF2 gene in Mastermind

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