DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD b... See more...

Aliases for DHX35 Gene

Aliases for DHX35 Gene

  • DEAH-Box Helicase 35 2 3 5
  • DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 35 2 3
  • DEAH (Asp-Glu-Ala-His) Box Polypeptide 35 2 3
  • Probable ATP-Dependent RNA Helicase DHX35 3 4
  • C20orf15 3 4
  • KAIA0875 2 3
  • DDX35 3 4
  • DEAH-Box Protein 35 3
  • DEAH Box Protein 35 4
  • EC 3.6.4.13 4
  • FLJ22759 2
  • EC 3.6.1 51
  • DHX35 5

External Ids for DHX35 Gene

Previous HGNC Symbols for DHX35 Gene

  • C20orf15
  • DDX35

Previous GeneCards Identifiers for DHX35 Gene

  • GC20P038229
  • GC20P038276
  • GC20P037024
  • GC20P037590
  • GC20P034328

Summaries for DHX35 Gene

Entrez Gene Summary for DHX35 Gene

  • DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The function of this gene product which is a member of this family, has not been determined. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2010]

GeneCards Summary for DHX35 Gene

DHX35 (DEAH-Box Helicase 35) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and helicase activity. An important paralog of this gene is DHX8.

UniProtKB/Swiss-Prot Summary for DHX35 Gene

Additional gene information for DHX35 Gene

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for DHX35 Gene

Genomics for DHX35 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for DHX35 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around DHX35 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for DHX35

Top Transcription factor binding sites by QIAGEN in the DHX35 gene promoter:
  • C/EBPalpha
  • Cart-1
  • FOXJ2
  • FOXJ2 (long isoform)
  • GATA-3
  • GR
  • GR-alpha
  • STAT5B
  • USF-1
  • USF1

Genomic Locations for DHX35 Gene

Genomic Locations for DHX35 Gene
chr20:38,962,299-39,039,723
(GRCh38/hg38)
Size:
77,425 bases
Orientation:
Plus strand
chr20:37,590,942-37,668,366
(GRCh37/hg19)
Size:
77,425 bases
Orientation:
Plus strand

Genomic View for DHX35 Gene

Genes around DHX35 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
DHX35 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for DHX35 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for DHX35 Gene

Proteins for DHX35 Gene

  • Protein details for DHX35 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9H5Z1-DHX35_HUMAN
    Recommended name:
    Probable ATP-dependent RNA helicase DHX35
    Protein Accession:
    Q9H5Z1
    Secondary Accessions:
    • A2RTX3
    • B4E0J0
    • F5GXM6
    • Q5THR0
    • Q9H4H7
    • Q9H6T6

    Protein attributes for DHX35 Gene

    Size:
    703 amino acids
    Molecular mass:
    78910 Da
    Quaternary structure:
    • Identified in the spliceosome C complex.
    SequenceCaution:
    • Sequence=BAB15166.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};

    Alternative splice isoforms for DHX35 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for DHX35 Gene

Selected DME Specific Peptides for DHX35 Gene

Q9H5Z1:
  • LDEAHERT
  • TQPRRVAA
  • AETSITI
  • FLTGQEE
  • CYRLYTE
  • DEAHERTL

Post-translational modifications for DHX35 Gene

  • Ubiquitination at Lys9, Lys326, Lys413, Lys435, and Lys591
  • Modification sites at PhosphoSitePlus

Domains & Families for DHX35 Gene

Gene Families for DHX35 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for DHX35 Gene

InterPro:
Blocks:
  • ATP-dependent helicase, DEAH-box
  • Helicase-associated region
ProtoNet:

Suggested Antigen Peptide Sequences for DHX35 Gene

GenScript: Design optimal peptide antigens:
  • DEAH box protein 35 (DHX35_HUMAN)
  • DEAH (Asp-Glu-Ala-His) box polypeptide 35 (Q5THQ7_HUMAN)
  • DEAH (Asp-Glu-Ala-His) box polypeptide 35 (Q5THQ9_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9H5Z1

UniProtKB/Swiss-Prot:

DHX35_HUMAN :
  • Belongs to the DEAD box helicase family. DEAH subfamily.
Family:
  • Belongs to the DEAD box helicase family. DEAH subfamily.
genes like me logo Genes that share domains with DHX35: view

Function for DHX35 Gene

Molecular function for DHX35 Gene

UniProtKB/Swiss-Prot Function:
May be involved in pre-mRNA splicing.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;.

Enzyme Numbers (IUBMB) for DHX35 Gene

Phenotypes From GWAS Catalog for DHX35 Gene

Gene Ontology (GO) - Molecular Function for DHX35 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0003723 RNA binding IBA 21873635
GO:0003724 RNA helicase activity IEA --
GO:0004386 helicase activity IEA --
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with DHX35: view
genes like me logo Genes that share phenotypes with DHX35: view

Animal Models for DHX35 Gene

MGI Knock Outs for DHX35:
  • Dhx35 Dhx35<tm1b(EUCOMM)Wtsi>

Animal Model Products

  • Taconic Biosciences Mouse Models for DHX35

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for DHX35

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for DHX35 Gene

Localization for DHX35 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for DHX35 gene
Compartment Confidence
nucleus 5
cytoskeleton 1
mitochondrion 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Centrosome (2)
  • Nuclear bodies (2)
  • Nucleoplasm (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for DHX35 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005622 intracellular IBA 21873635
GO:0005681 spliceosomal complex IEA --
GO:0071013 catalytic step 2 spliceosome IBA,IDA 11991638
genes like me logo Genes that share ontologies with DHX35: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for DHX35 Gene

Pathways & Interactions for DHX35 Gene

PathCards logo

SuperPathways for DHX35 Gene

No Data Available

Gene Ontology (GO) - Biological Process for DHX35 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000398 mRNA splicing, via spliceosome IC 11991638
GO:0001701 in utero embryonic development IEA --
GO:0006397 mRNA processing IEA --
GO:0008380 RNA splicing IEA --
genes like me logo Genes that share ontologies with DHX35: view

No data available for Pathways by source and SIGNOR curated interactions for DHX35 Gene

Drugs & Compounds for DHX35 Gene

(3) Drugs for DHX35 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Phosphoric acid Approved Pharma 0
Water Approved Pharma 0
ATP Investigational Nutra Agonist, Activator, Partial agonist, Antagonist, Full agonist, Gating inhibitor, Pore Blocker, Potentiation 0

(1) Additional Compounds for DHX35 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • 5'-Adenylphosphoric acid
  • Adenosine 5'-diphosphate
  • ADENOSINE-5'-diphosphATE
  • H3ADP
  • 5'-Adenylphosphate
Agonist, Full agonist, Partial agonist, Gating inhibitor, Antagonist 58-64-0
genes like me logo Genes that share compounds with DHX35: view

Transcripts for DHX35 Gene

mRNA/cDNA for DHX35 Gene

2 REFSEQ mRNAs :
14 NCBI additional mRNA sequence :
8 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for DHX35

Alternative Splicing Database (ASD) splice patterns (SP) for DHX35 Gene

No ASD Table

Relevant External Links for DHX35 Gene

GeneLoc Exon Structure for
DHX35

Expression for DHX35 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for DHX35 Gene

Protein differential expression in normal tissues from HIPED for DHX35 Gene

This gene is overexpressed in Peripheral blood mononuclear cells (34.8) and Placenta (13.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for DHX35 Gene



Protein tissue co-expression partners for DHX35 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for DHX35

SOURCE GeneReport for Unigene cluster for DHX35 Gene:

Hs.444520

Evidence on tissue expression from TISSUES for DHX35 Gene

  • Nervous system(4.6)
  • Intestine(4.2)
genes like me logo Genes that share expression patterns with DHX35: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for DHX35 Gene

Orthologs for DHX35 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for DHX35 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia DHX35 30 31
  • 99 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia DHX35 30 31
  • 91.3 (n)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia DHX35 31
  • 91 (a)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia DHX35 31
  • 91 (a)
OneToOne
Cow
(Bos Taurus)
Mammalia DHX35 30 31
  • 90.31 (n)
OneToOne
Mouse
(Mus musculus)
Mammalia Dhx35 30 17 31
  • 88.35 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Dhx35 30
  • 88.35 (n)
Chicken
(Gallus gallus)
Aves DHX35 30 31
  • 79.77 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia DHX35 31
  • 77 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia dhx35 30
  • 73.9 (n)
Zebrafish
(Danio rerio)
Actinopterygii DHX35 31
  • 77 (a)
OneToOne
Fruit Fly
(Drosophila melanogaster)
Insecta CG3225 30 31
  • 54.59 (n)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP010872 30
  • 54.34 (n)
Worm
(Caenorhabditis elegans)
Secernentea Y67D2.6 30 31
  • 56.68 (n)
OneToOne
Thale Cress
(Arabidopsis thaliana)
eudicotyledons AT4G18465 30
  • 54.98 (n)
Rice
(Oryza sativa)
Liliopsida Os01g0212100 30
  • 54.17 (n)
Bread mold
(Neurospora crassa)
Ascomycetes NCU06735 30
  • 55.17 (n)
Fission Yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes SPAC20H4.09 30
  • 49.76 (n)
Species where no ortholog for DHX35 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for DHX35 Gene

ENSEMBL:
Gene Tree for DHX35 (if available)
TreeFam:
Gene Tree for DHX35 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for DHX35: view image

Paralogs for DHX35 Gene

(11) SIMAP similar genes for DHX35 Gene using alignment to 5 proteins:

  • DHX35_HUMAN
  • F2Z2Z2_HUMAN
  • Q5THQ7_HUMAN
  • Q5THQ9_HUMAN
  • Q5THR1_HUMAN
genes like me logo Genes that share paralogs with DHX35: view

Variants for DHX35 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for DHX35 Gene

SNP ID Clinical significance and condition Chr 20 pos Variation AA Info Type
rs36053162 - p.Ile189Thr
rs3752302 - p.Pro703Leu

Additional dbSNP identifiers (rs#s) for DHX35 Gene

Structural Variations from Database of Genomic Variants (DGV) for DHX35 Gene

Variant ID Type Subtype PubMed ID
esv3645793 CNV gain 21293372
esv3645794 CNV loss 21293372
esv3645795 CNV gain 21293372
esv3645796 CNV loss 21293372
esv3645797 CNV gain 21293372
esv3645798 CNV loss 21293372
esv3645799 CNV loss 21293372
nsv833973 CNV loss 17160897
nsv979433 CNV duplication 23825009

Variation tolerance for DHX35 Gene

Residual Variation Intolerance Score: 15.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 8.71; 86.54% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for DHX35 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
DHX35

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for DHX35 Gene

Disorders for DHX35 Gene

Additional Disease Information for DHX35

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform

No disorders were found for DHX35 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for DHX35 Gene

Publications for DHX35 Gene

  1. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PMID: 20379614) Rose JE … Uhl GR (Molecular medicine (Cambridge, Mass.) 2010) 3 41
  2. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4
  3. Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. (PMID: 11991638) Jurica MS … Moore MJ (RNA (New York, N.Y.) 2002) 3 4
  4. The DNA sequence and comparative analysis of human chromosome 20. (PMID: 11780052) Deloukas P … Rogers J (Nature 2001) 3 4
  5. An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations. (PMID: 29568061) Liu X … Varjosalo M (Nature communications 2018) 3

Products for DHX35 Gene

Sources for DHX35 Gene