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Aliases for DDX25 Gene

Aliases for DDX25 Gene

  • DEAD-Box Helicase 25 2 3 5
  • Gonadotropin-Regulated Testicular RNA Helicase 2 3 4
  • DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 25 2 3
  • DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 25 2 3
  • DEAD (Asp-Glu-Ala-Asp) Box Helicase 25 2 3
  • DEAD Box Protein 25 3 4
  • GRTH 3 4
  • ATP-Dependent RNA Helicase DDX25 3
  • EC 4
  • EC 3.6.1 56

External Ids for DDX25 Gene

Previous GeneCards Identifiers for DDX25 Gene

  • GC11P127774
  • GC11P127287
  • GC11P125806
  • GC11P125312
  • GC11P125279
  • GC11P121716
  • GC11P125773

Summaries for DDX25 Gene

Entrez Gene Summary for DDX25 Gene

  • DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The encoded protein is a gonadotropin-regulated and developmentally expressed testicular RNA helicase. It may serve to maintain testicular functions related to steroidogenesis and spermatogenesis. [provided by RefSeq, Jul 2008]

GeneCards Summary for DDX25 Gene

DDX25 (DEAD-Box Helicase 25) is a Protein Coding gene. Diseases associated with DDX25 include Hydrolethalus Syndrome 1. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and helicase activity. An important paralog of this gene is ENSG00000260537.

UniProtKB/Swiss-Prot for DDX25 Gene

  • ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development (By similarity).

Additional gene information for DDX25 Gene

No data available for CIViC summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for DDX25 Gene

Genomics for DDX25 Gene

GeneHancer (GH) Regulatory Elements for DDX25 Gene

Promoters and enhancers for DDX25 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH11I125901 Promoter/Enhancer 1.9 EPDnew Ensembl ENCODE 604 -0.4 -351 2 PKNOX1 ATF1 ARID4B SIN3A DMAP1 YY1 ETS1 POLR2B ZNF766 ZNF207 DDX25 PUS3 ENSG00000254790 ENSG00000254694 FAM118B RPUSD4 PIR36090 ENSG00000255027
GH11I125904 Promoter/Enhancer 1.6 EPDnew Ensembl ENCODE 563.5 +1.0 1020 0.3 RING1 MXI1 USF1 MAX SIN3A USF2 POLR2A EZH2 DDX25 PUS3 ENSG00000254790 FAM118B RPUSD4 ENSG00000254694 GC11P125928 ENSG00000255027
GH11I125568 Promoter/Enhancer 2 EPDnew Ensembl ENCODE 8.5 -333.3 -333315 2.5 HDGF PKNOX1 FOXA2 ARNT ARID4B SIN3A DMAP1 ZNF2 YY1 ZNF213 EI24 FEZ1 DDX25 PUS3 RNU6-1156P STT3A-AS1
GH11I125591 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE 7.5 -310.6 -310614 3.1 HDGF PKNOX1 SMAD1 ARNT ARID4B SIN3A DMAP1 YY1 SLC30A9 POLR2B STT3A STT3A-AS1 HP11014 TMEM218 CHEK1 MSANTD2 ENSG00000254509 TIRAP ENSG00000254694 EI24
GH11I125800 Enhancer 0.4 FANTOM5 38.7 -103.0 -102969 0.4 REST ZNF507 DDX25 CHEK1 PATE4 HYLS1 PATE1 PATE2 GC11P125812 PATE3
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around DDX25 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the DDX25 gene promoter:

Genomic Locations for DDX25 Gene

Genomic Locations for DDX25 Gene
40,355 bases
Plus strand

Genomic View for DDX25 Gene

Genes around DDX25 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
DDX25 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for DDX25 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for DDX25 Gene

Proteins for DDX25 Gene

  • Protein details for DDX25 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    ATP-dependent RNA helicase DDX25
    Protein Accession:
    Secondary Accessions:
    • B2R6Z0
    • Q5XVN2
    • Q86W81
    • Q8IYP1

    Protein attributes for DDX25 Gene

    483 amino acids
    Molecular mass:
    54692 Da
    Quaternary structure:
    No Data Available

    Three dimensional structures from OCA and Proteopedia for DDX25 Gene

    Alternative splice isoforms for DDX25 Gene


neXtProt entry for DDX25 Gene

Selected DME Specific Peptides for DDX25 Gene


Post-translational modifications for DDX25 Gene

  • Phosphorylated on threonine residues. The phosphorylated form is found in the cytoplasm but not in the nucleus (By similarity).

Domains & Families for DDX25 Gene

Gene Families for DDX25 Gene

Human Protein Atlas (HPA):
  • Enzymes
  • Plasma proteins
  • Predicted intracellular proteins
  • Predicted secreted proteins

Suggested Antigen Peptide Sequences for DDX25 Gene

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the DEAD box helicase family.
  • Belongs to the DEAD box helicase family.
genes like me logo Genes that share domains with DDX25: view

Function for DDX25 Gene

Molecular function for DDX25 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
ATP + H(2)O = ADP + phosphate.
UniProtKB/Swiss-Prot Function:
ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development (By similarity).
UniProtKB/Swiss-Prot Induction:
Up-regulated at transcriptional level by chorionic gonadotropin via cyclic AMP-induced androgen formation in the Leydig cell.

Enzyme Numbers (IUBMB) for DDX25 Gene

Phenotypes From GWAS Catalog for DDX25 Gene

Gene Ontology (GO) - Molecular Function for DDX25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003676 nucleic acid binding IEA --
GO:0003723 RNA binding IEA --
GO:0004004 ATP-dependent RNA helicase activity ISS,IEA --
GO:0004386 helicase activity IEA --
GO:0005524 ATP binding ISS,IEA --
genes like me logo Genes that share ontologies with DDX25: view
genes like me logo Genes that share phenotypes with DDX25: view

Animal Models for DDX25 Gene

MGI Knock Outs for DDX25:

Animal Model Products

CRISPR Products

miRNA for DDX25 Gene

miRTarBase miRNAs that target DDX25

Inhibitory RNA Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for DDX25 Gene

Localization for DDX25 Gene

Subcellular locations from UniProtKB/Swiss-Prot for DDX25 Gene

Cytoplasm. Nucleus. Note=Detected in both cytoplasm and nucleus of testicular cells. Also detected in chromatoid bodies of round spermatids (By similarity). {ECO:0000250}.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for DDX25 gene
Compartment Confidence
nucleus 5
cytosol 3

Gene Ontology (GO) - Cellular Components for DDX25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus ISS --
GO:0005730 nucleolus IBA --
GO:0005737 cytoplasm ISS,IBA --
GO:0033391 chromatoid body ISS,IEA --
GO:0071013 catalytic step 2 spliceosome IBA --
genes like me logo Genes that share ontologies with DDX25: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for DDX25 Gene

Pathways & Interactions for DDX25 Gene

SuperPathways for DDX25 Gene

No Data Available

Gene Ontology (GO) - Biological Process for DDX25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006406 mRNA export from nucleus IEA,ISS --
GO:0006417 regulation of translation ISS --
GO:0006810 transport IEA --
GO:0007275 multicellular organism development IEA --
GO:0007283 spermatogenesis IEA --
genes like me logo Genes that share ontologies with DDX25: view

No data available for Pathways by source and SIGNOR curated interactions for DDX25 Gene

Drugs & Compounds for DDX25 Gene

(4) Drugs for DDX25 Gene - From: HMDB and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Phosphoric acid Approved Pharma 0
Water Approved Pharma 0
ATP Investigational Nutra Agonist 0

(1) Additional Compounds for DDX25 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0
genes like me logo Genes that share compounds with DDX25: view

Transcripts for DDX25 Gene

Unigene Clusters for DDX25 Gene

DEAD (Asp-Glu-Ala-Asp) box helicase 25:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Alternative Splicing Database (ASD) splice patterns (SP) for DDX25 Gene

No ASD Table

Relevant External Links for DDX25 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for DDX25 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for DDX25 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for DDX25 Gene

This gene is overexpressed in Testis (x9.9), Brain - Cortex (x4.1), and Brain - Frontal Cortex (BA9) (x4.0).

Protein differential expression in normal tissues from HIPED for DDX25 Gene

This gene is overexpressed in Testis (59.8) and Heart (6.8).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for DDX25 Gene

Protein tissue co-expression partners for DDX25 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of DDX25 Gene:


SOURCE GeneReport for Unigene cluster for DDX25 Gene:


mRNA Expression by UniProt/SwissProt for DDX25 Gene:

Tissue specificity: Highly expressed in the Leydig and germ cells of the testis and weakly expressed in the pituitary and hypothalamus.

Evidence on tissue expression from TISSUES for DDX25 Gene

  • Nervous system(4.6)
genes like me logo Genes that share expression patterns with DDX25: view

Primer Products

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for DDX25 Gene

Orthologs for DDX25 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for DDX25 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia DDX25 33 34
  • 99.72 (n)
(Canis familiaris)
Mammalia DDX25 33 34
  • 90.97 (n)
(Monodelphis domestica)
Mammalia DDX25 34
  • 90 (a)
(Bos Taurus)
Mammalia DDX25 33 34
  • 89.03 (n)
(Mus musculus)
Mammalia Ddx25 33 16 34
  • 87.65 (n)
(Rattus norvegicus)
Mammalia Ddx25 33
  • 87.51 (n)
(Ornithorhynchus anatinus)
Mammalia DDX25 34
  • 81 (a)
(Gallus gallus)
Aves DDX25 33 34
  • 68.16 (n)
(Anolis carolinensis)
Reptilia DDX25 34
  • 64 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia ddx25 33
  • 66.29 (n)
(Danio rerio)
Actinopterygii zgc:193690 34
  • 60 (a)
fruit fly
(Drosophila melanogaster)
Insecta Dbp80 35 34
  • 58 (a)
(Caenorhabditis elegans)
Secernentea T07D4.4a 35
  • 48 (a)
T07D4.4b 35
  • 48 (a)
T07D4.4c 35
  • 48 (a)
ddx-19 34
  • 21 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes DBP5 34
  • 41 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.8406 34
  • 55 (a)
Species where no ortholog for DDX25 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for DDX25 Gene

Gene Tree for DDX25 (if available)
Gene Tree for DDX25 (if available)

Paralogs for DDX25 Gene

Paralogs for DDX25 Gene Pseudogenes for DDX25 Gene

genes like me logo Genes that share paralogs with DDX25: view

Variants for DDX25 Gene

Sequence variations from dbSNP and Humsavar for DDX25 Gene

SNP ID Clin Chr 11 pos Variation AA Info Type
rs1000030391 -- 125,905,970(+) C/T intron_variant
rs1000478233 -- 125,918,533(+) T/C intron_variant
rs1000594025 -- 125,912,401(+) T/C intron_variant
rs1000750411 -- 125,915,537(+) A/C intron_variant
rs1000774965 -- 125,906,742(+) C/G intron_variant

Structural Variations from Database of Genomic Variants (DGV) for DDX25 Gene

Variant ID Type Subtype PubMed ID
nsv556525 CNV gain 21841781
nsv535 CNV deletion 18451855
esv32964 CNV gain+loss 17666407
dgv248n67 CNV gain 20364138

Variation tolerance for DDX25 Gene

Residual Variation Intolerance Score: 21.8% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.73; 15.41% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for DDX25 Gene

Human Gene Mutation Database (HGMD)
SNPedia medical, phenotypic, and genealogical associations of SNPs for

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for DDX25 Gene

Disorders for DDX25 Gene

MalaCards: The human disease database

(1) MalaCards diseases for DDX25 Gene - From: HGMD and DISEASES

Disorder Aliases PubMed IDs
hydrolethalus syndrome 1
  • hls1
- elite association - COSMIC cancer census association via MalaCards
Search DDX25 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for DDX25

Genetic Association Database
Human Genome Epidemiology Navigator
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
genes like me logo Genes that share disorders with DDX25: view

No data available for UniProtKB/Swiss-Prot and Genatlas for DDX25 Gene

Publications for DDX25 Gene

  1. A novel gonadotropin-regulated testicular RNA helicase. A new member of the dead-box family. (PMID: 10608860) Tang PZ … Dufau ML (The Journal of biological chemistry 1999) 2 3 4 22 58
  2. Polymorphism of the GRTH/DDX25 gene in normal and infertile Japanese men: a missense mutation associated with loss of GRTH phosphorylation. (PMID: 17848414) Tsai-Morris CH … Dufau ML (Molecular human reproduction 2007) 3 22 44 58
  3. Single nucleotide polymorphisms of the gonadotrophin-regulated testicular helicase (GRTH) gene may be associated with the human spermatogenesis impairment. (PMID: 16293649) A Z … Zhang W (Human reproduction (Oxford, England) 2006) 3 22 44 58
  4. Evaluation of 172 candidate polymorphisms for association with oligozoospermia or azoospermia in a large cohort of men of European descent. (PMID: 20378615) Aston KI … Carrell DT (Human reproduction (Oxford, England) 2010) 3 44 58
  5. Gonadotropin-regulated testicular RNA helicase (GRTH/DDX25): a multifunctional protein essential for spermatogenesis. (PMID: 19875492) Tsai-Morris CH … Dufau ML (Journal of andrology 2010) 3 22 58

Products for DDX25 Gene

Sources for DDX25 Gene

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