DDX25 Gene (Protein Coding)

DEAD-Box Helicase 25

GC11P125903
GIFtS:
40
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD... See more...

Aliases for DDX25 Gene

Aliases for DDX25 Gene

  • DEAD-Box Helicase 25 2 3 5
  • Gonadotropin-Regulated Testicular RNA Helicase 2 3 4
  • GRTH 2 3 4
  • DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 25 2 3
  • DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 25 2 3
  • DEAD (Asp-Glu-Ala-Asp) Box Helicase 25 2 3
  • ATP-Dependent RNA Helicase DDX25 3 4
  • DEAD Box Protein 25 3 4
  • Epididymis Secretory Sperm Binding Protein 3
  • EC 3.6.4.13 4
  • EC 3.6.1 51
  • DDX25 5

External Ids for DDX25 Gene

Previous GeneCards Identifiers for DDX25 Gene

  • GC11P127774
  • GC11P127287
  • GC11P125806
  • GC11P125312
  • GC11P125279
  • GC11P121716
  • GC11P125773

Summaries for DDX25 Gene

Entrez Gene Summary for DDX25 Gene

  • DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The encoded protein is a gonadotropin-regulated and developmentally expressed testicular RNA helicase. It may serve to maintain testicular functions related to steroidogenesis and spermatogenesis. [provided by RefSeq, Jul 2008]

GeneCards Summary for DDX25 Gene

DDX25 (DEAD-Box Helicase 25) is a Protein Coding gene. Diseases associated with DDX25 include Male Infertility. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and helicase activity. An important paralog of this gene is ENSG00000260537.

UniProtKB/Swiss-Prot Summary for DDX25 Gene

  • ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development (By similarity).

Additional gene information for DDX25 Gene

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for DDX25 Gene

Genomics for DDX25 Gene

GeneHancer (GH) Regulatory Elements Pubs

Download GeneHancer 2017 data
Promoters and enhancers for DDX25 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around DDX25 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for DDX25

Top Transcription factor binding sites by QIAGEN in the DDX25 gene promoter:
  • CBF(2)
  • CBF-B
  • E47
  • Pax-2
  • Pax-2a
  • Pax-6
  • POU3F2
  • POU3F2 (N-Oct-5a)
  • POU3F2 (N-Oct-5b)
  • TBP

Genomic Locations for DDX25 Gene

Latest Assembly
chr11:125,903,328-125,943,702
(GRCh38/hg38)
Size:
40,375 bases
Orientation:
Plus strand

Previous Assembly
chr11:125,773,223-125,798,738
(GRCh37/hg19 by Entrez Gene)
Size:
25,516 bases
Orientation:
Plus strand

chr11:125,773,271-125,793,158
(GRCh37/hg19 by Ensembl)
Size:
19,888 bases
Orientation:
Plus strand

Genomic View for DDX25 Gene

Genes around DDX25 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
DDX25 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for DDX25 Gene
GeneLoc Logo Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for DDX25 Gene

Proteins for DDX25 Gene

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  • Protein details for DDX25 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9UHL0-DDX25_HUMAN
    Recommended name:
    ATP-dependent RNA helicase DDX25
    Protein Accession:
    Q9UHL0
    Secondary Accessions:
    • B2R6Z0
    • Q5XVN2
    • Q86W81
    • Q8IYP1

    Protein attributes for DDX25 Gene

    Size:
    483 amino acids
    Molecular mass:
    54692 Da
    Quaternary structure:
    No Data Available

    Three dimensional structures from OCA and Proteopedia for DDX25 Gene

    Alternative splice isoforms for DDX25 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for DDX25 Gene

Protein Expression for DDX25 Gene

See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB

Selected DME Specific Peptides for DDX25 Gene

Q9UHL0:
  • KVLITTN
  • IGTPGTVLDWC
  • DVVDLAANSLLNKLI
  • FVLDEAD
  • ITIGQAIIFCQTRRNAKWLTVEM
  • HRIGRTGR
  • GTGKTAAF
  • YLHRIGR
  • SPLYSVK
  • YAMGFNRPSKIQE
  • ALPMMLA
  • QALCNIYG
  • CQMLLFSATFEDSVW
  • TTNVCARGIDV
  • PDPNVIKL

Post-translational modifications for DDX25 Gene

  • Phosphorylated on threonine residues. The phosphorylated form is found in the cytoplasm but not in the nucleus (By similarity).
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for DDX25 Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for DDX25 Gene

Gene Families for DDX25 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for DDX25 Gene

InterPro:
Blocks:
  • ATP-dependent helicase, DEAD-box
ProtoNet:

Suggested Antigen Peptide Sequences for DDX25 Gene

GenScript: Design optimal peptide antigens:
  • DDX25 (B4YF11_HUMAN)
  • Gonadotropin-regulated testicular RNA helicase (DDX25_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9UHL0

UniProtKB/Swiss-Prot:

DDX25_HUMAN :
  • Belongs to the DEAD box helicase family.
Family:
  • Belongs to the DEAD box helicase family.
genes like me logo Genes that share domains with DDX25: view

Function for DDX25 Gene

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  6. Cell Lines for research

Molecular function for DDX25 Gene

UniProtKB/Swiss-Prot Function:
ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development (By similarity).
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;.
UniProtKB/Swiss-Prot Induction:
Up-regulated at transcriptional level by chorionic gonadotropin via cyclic AMP-induced androgen formation in the Leydig cell.

Enzyme Numbers (IUBMB) for DDX25 Gene

Phenotypes From GWAS Catalog for DDX25 Gene

Gene Ontology (GO) - Molecular Function for DDX25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0003676 nucleic acid binding IEA --
GO:0003723 RNA binding IBA 21873635
GO:0003724 RNA helicase activity IEA,ISS --
GO:0004386 helicase activity IEA --
genes like me logo Genes that share ontologies with DDX25: view
genes like me logo Genes that share phenotypes with DDX25: view

Animal Models for DDX25 Gene

MGI Knock Outs for DDX25:

Animal Models for research

miRNA for DDX25 Gene

miRTarBase miRNAs that target DDX25

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for DDX25

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for DDX25 Gene

Localization for DDX25 Gene

Subcellular locations from UniProtKB/Swiss-Prot for DDX25 Gene

Cytoplasm. Nucleus. Note=Detected in both cytoplasm and nucleus of testicular cells. Also detected in chromatoid bodies of round spermatids (By similarity). {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for DDX25 gene
Compartment Confidence
nucleus 4
cytosol 3
extracellular 1
cytoskeleton 1
mitochondrion 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (3)
  • Nucleoplasm (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for DDX25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IBA,ISS --
GO:0005737 cytoplasm ISS --
GO:0010494 cytoplasmic stress granule IBA 21873635
GO:0033391 chromatoid body IEA,ISS --
genes like me logo Genes that share ontologies with DDX25: view

Pathways & Interactions for DDX25 Gene

PathCards logo

SuperPathways for DDX25 Gene

No Data Available

Gene Ontology (GO) - Biological Process for DDX25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006406 mRNA export from nucleus IEA,ISS --
GO:0006417 regulation of translation ISS --
GO:0007275 multicellular organism development IEA --
GO:0007283 spermatogenesis IEA --
GO:0007286 spermatid development IEA,ISS --
genes like me logo Genes that share ontologies with DDX25: view

No data available for Pathways by source and SIGNOR curated interactions for DDX25 Gene

Drugs & Compounds for DDX25 Gene

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(4) Drugs for DDX25 Gene - From: HMDB and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Water Approved Pharma 0
Phosphate Experimental Pharma 0
ATP Investigational Nutra 0

(1) Additional Compounds for DDX25 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • 5'-Adenylphosphoric acid
  • Adenosine 5'-diphosphate
  • ADENOSINE-5'-diphosphATE
  • H3ADP
  • 5'-Adenylphosphate
 58-64-0
genes like me logo Genes that share compounds with DDX25: view

Transcripts for DDX25 Gene

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mRNA/cDNA for DDX25 Gene

2 REFSEQ mRNAs :
13 NCBI additional mRNA sequence :
10 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for DDX25

Alternative Splicing Database (ASD) splice patterns (SP) for DDX25 Gene

No ASD Table

Relevant External Links for DDX25 Gene

GeneLoc Exon Structure for
DDX25

Expression for DDX25 Gene

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mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for DDX25 Gene

mRNA expression in normal human tissues for DDX25 Gene
mRNA expression by BioGPS for DDX25 GenemRNA expression by GTEx for DDX25 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for DDX25 Gene

This gene is overexpressed in Testis (x9.9), Brain - Cortex (x4.1), and Brain - Frontal Cortex (BA9) (x4.0).

Protein differential expression in normal tissues from HIPED for DDX25 Gene

This gene is overexpressed in Testis (59.8) and Heart (6.8).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for DDX25 Gene



Protein tissue co-expression partners for DDX25 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for DDX25

SOURCE GeneReport for Unigene cluster for DDX25 Gene:

Hs.420263

mRNA Expression by UniProt/SwissProt for DDX25 Gene:

Q9UHL0-DDX25_HUMAN
Tissue specificity: Highly expressed in the Leydig and germ cells of the testis and weakly expressed in the pituitary and hypothalamus.

Evidence on tissue expression from TISSUES for DDX25 Gene

  • Nervous system(4.7)
genes like me logo Genes that share expression patterns with DDX25: view

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for DDX25 Gene

Orthologs for DDX25 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for DDX25 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
 Mammalia DDX25 30 31
  • 99.72 (n)
 OneToOne
Dog
(Canis familiaris)
 Mammalia DDX25 30 31
  • 90.97 (n)
 OneToOne
Oppossum
(Monodelphis domestica)
 Mammalia DDX25 31
  • 90 (a)
 OneToOne
Cow
(Bos Taurus)
 Mammalia DDX25 30 31
  • 89.03 (n)
 OneToOne
Mouse
(Mus musculus)
 Mammalia Ddx25 30 17 31
  • 87.65 (n)
 OneToOne
Rat
(Rattus norvegicus)
 Mammalia Ddx25 30
  • 87.51 (n)
Platypus
(Ornithorhynchus anatinus)
 Mammalia DDX25 31
  • 81 (a)
 OneToOne
Chicken
(Gallus gallus)
 Aves DDX25 30 31
  • 68.16 (n)
 OneToOne
Lizard
(Anolis carolinensis)
 Reptilia DDX25 31
  • 64 (a)
 OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
 Amphibia ddx25 30
  • 66.29 (n)
Zebrafish
(Danio rerio)
 Actinopterygii zgc:193690 31
  • 60 (a)
 ManyToMany
Fruit Fly
(Drosophila melanogaster)
 Insecta Dbp80 31 32
  • 51 (a)
 OneToMany
Worm
(Caenorhabditis elegans)
 Secernentea T07D4.4a 32
  • 48 (a)
T07D4.4b 32
  • 48 (a)
T07D4.4c 32
  • 48 (a)
ddx-19 31
  • 21 (a)
 OneToMany
Baker's yeast
(Saccharomyces cerevisiae)
 Saccharomycetes DBP5 31
  • 41 (a)
 OneToMany
Sea Squirt
(Ciona savignyi)
 Ascidiacea CSA.8406 31
  • 55 (a)
 OneToMany
Species where no ortholog for DDX25 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for DDX25 Gene

ENSEMBL:
Gene Tree for DDX25 (if available)
TreeFam:
Gene Tree for DDX25 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for DDX25: view image

Paralogs for DDX25 Gene

Paralogs for DDX25 Gene

(16) SIMAP similar genes for DDX25 Gene using alignment to 6 proteins:

  • DDX25_HUMAN
  • B4YF11_HUMAN
  • E9PR46_HUMAN
  • H0YD26_HUMAN
  • J3QL17_HUMAN
  • J3QRJ6_HUMAN

Pseudogenes.org Pseudogenes for DDX25 Gene

genes like me logo Genes that share paralogs with DDX25: view

Variants for DDX25 Gene

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Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for DDX25 Gene

SNP ID Clinical significance and condition Chr 11 pos Variation AA Info Type
rs116333528 Benign: not provided 125,910,358(+) C/T
NM_013264.5(DDX25):c.508-6C>T 		INTRON
rs34007659 Benign: not provided 125,908,410(+) C/T
NM_013264.5(DDX25):c.414C>T (p.Asn138=) 		SYNONYMOUS

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for DDX25 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for DDX25 Gene

Variant ID Type Subtype PubMed ID
dgv248n67 CNV gain 20364138
esv32964 CNV gain+loss 17666407
nsv535 CNV deletion 18451855
nsv556525 CNV gain 21841781

Variation tolerance for DDX25 Gene

Residual Variation Intolerance Score: 21.8% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.73; 15.41% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for DDX25 Gene

Human Gene Mutation Database (HGMD)
DDX25
SNPedia medical, phenotypic, and genealogical associations of SNPs for
DDX25

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for DDX25 Gene

Disorders for DDX25 Gene

MalaCards: The human disease database

(1) MalaCards diseases for DDX25 Gene - From: COP and GCD

Disorder Aliases PubMed IDs
male infertility
  • infertility male
- elite association - COSMIC cancer census association via MalaCards
Search DDX25 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for DDX25

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
Atlas
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
Open Targets Platform
genes like me logo Genes that share disorders with DDX25: view

No data available for UniProtKB/Swiss-Prot and Genatlas for DDX25 Gene

Publications for DDX25 Gene

  1. A novel gonadotropin-regulated testicular RNA helicase. A new member of the dead-box family. (PMID: 10608860) Tang PZ … Dufau ML (The Journal of biological chemistry 1999) 2 3 4 23
  2. Polymorphism of the GRTH/DDX25 gene in normal and infertile Japanese men: a missense mutation associated with loss of GRTH phosphorylation. (PMID: 17848414) Tsai-Morris CH … Dufau ML (Molecular human reproduction 2007) 3 23 41
  3. Single nucleotide polymorphisms of the gonadotrophin-regulated testicular helicase (GRTH) gene may be associated with the human spermatogenesis impairment. (PMID: 16293649) A Z … Zhang W (Human reproduction (Oxford, England) 2006) 3 23 41
  4. Gonadotropin-regulated testicular RNA helicase (GRTH/DDX25): a multifunctional protein essential for spermatogenesis. (PMID: 19875492) Tsai-Morris CH … Dufau ML (Journal of andrology 2010) 3 23
  5. Evaluation of 172 candidate polymorphisms for association with oligozoospermia or azoospermia in a large cohort of men of European descent. (PMID: 20378615) Aston KI … Carrell DT (Human reproduction (Oxford, England) 2010) 3 41

ExternalLinks

View latest publications for DDX25 gene in Mastermind

Products for DDX25 Gene

Sources for DDX25 Gene