This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/pr... See more...

Aliases for DCLK2 Gene

Aliases for DCLK2 Gene

  • Doublecortin Like Kinase 2 2 3 5
  • DCDC3B 2 3 4
  • DCK2 2 3 4
  • Doublecortin Domain-Containing Protein 3B 3 4
  • Doublecortin-Like And CAM Kinase-Like 2 3 4
  • Serine/Threonine-Protein Kinase DCLK2 3 4
  • Doublecortin And CaM Kinase-Like 2 2 3
  • CaMK-Like CREB Regulatory Kinase 2 3 4
  • Doublecortin-Like Kinase 2 2 4
  • EC 2.7.11.1 4 50
  • CLICK-II 3 4
  • DCAMKL2 3 4
  • CLICK2 3 4
  • DCDC3 2 3
  • CL2 3 4
  • EC 2.7.11 50
  • MGC45428 2
  • CLIK2 3
  • DCLK2 5

External Ids for DCLK2 Gene

Previous HGNC Symbols for DCLK2 Gene

  • DCAMKL2

Previous GeneCards Identifiers for DCLK2 Gene

  • GC04P151219
  • GC04P150999
  • GC04P146723

Summaries for DCLK2 Gene

Entrez Gene Summary for DCLK2 Gene

  • This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Sep 2010]

GeneCards Summary for DCLK2 Gene

DCLK2 (Doublecortin Like Kinase 2) is a Protein Coding gene. Diseases associated with DCLK2 include Lissencephaly and Band Heterotopia. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is DCLK1.

UniProtKB/Swiss-Prot Summary for DCLK2 Gene

  • Protein kinase with a significantly reduced C(a2+)/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm (By similarity).

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for DCLK2 Gene

Genomics for DCLK2 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for DCLK2 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH04J150077 Promoter/Enhancer 1.9 EPDnew Ensembl ENCODE CraniofacialAtlas 270.1 +1.7 1656 5.8 CEBPA JUND ZIC2 BCL11A ZNF217 FOXA2 CTBP1 KLF9 SP2 IRF4 DCLK2 HSALNG0037818 piR-38580-229 MAB21L2
GH04J150175 Enhancer 1.1 VISTA Ensembl ENCODE CraniofacialAtlas 25.8 +98.6 98599 3.7 JUND POLR2A EP300 HSALNG0037821 DCLK2 RF00066-144 RNU7-194P MAB21L2
GH04J150126 Enhancer 0.8 Ensembl ENCODE 34 +48.8 48755 3.6 JUND EBF1 CBFB BATF MAFK STAT5A ATF2 JUNB POLR2A CREM DCLK2 HQ292177 lnc-MAB21L2-6 HSALNG0037819 MAB21L2
GH04J150119 Enhancer 0.6 Ensembl 32.1 +41.4 41356 1.6 JUND FOXA1 FOXA2 TEAD3 FOSL2 PRDM6 SOX5 SMARCE1 DPF2 ARID3A DCLK2 lnc-MAB21L2-6 HQ292177 HSALNG0037819 MAB21L2
GH04J150130 Enhancer 0.6 Ensembl ENCODE 34 +52.7 52730 1.7 JUND POLR2A EP300 HQ292177 lnc-MAB21L2-6 DCLK2 HSALNG0037821 MAB21L2
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around DCLK2 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for DCLK2

Top Transcription factor binding sites by QIAGEN in the DCLK2 gene promoter:
  • AREB6
  • Bach2
  • C/EBPalpha
  • HEN1
  • Hlf
  • IRF-2
  • LCR-F1
  • Pax-2
  • Pax-2a
  • STAT1beta

Genomic Locations for DCLK2 Gene

Latest Assembly
chr4:150,078,445-150,257,449
(GRCh38/hg38)
Size:
179,005 bases
Orientation:
Plus strand

Previous Assembly
chr4:150,999,597-151,178,590
(GRCh37/hg19 by Entrez Gene)
Size:
178,994 bases
Orientation:
Plus strand

chr4:150,999,426-151,178,609
(GRCh37/hg19 by Ensembl)
Size:
179,184 bases
Orientation:
Plus strand

Genomic View for DCLK2 Gene

Genes around DCLK2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
DCLK2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for DCLK2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for DCLK2 Gene

Proteins for DCLK2 Gene

  • Protein details for DCLK2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8N568-DCLK2_HUMAN
    Recommended name:
    Serine/threonine-protein kinase DCLK2
    Protein Accession:
    Q8N568
    Secondary Accessions:
    • C9J5Q9
    • Q59GC8
    • Q8N399

    Protein attributes for DCLK2 Gene

    Size:
    766 amino acids
    Molecular mass:
    83606 Da
    Quaternary structure:
    • Binds to and stabilizes microtubules. Interacts with MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK9/JNK2, PPP1R9B/NEURABIN-2 and actin.
    SequenceCaution:
    • Sequence=BAD92418.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
    Miscellaneous:
    • [Isoform 2]: By homology to mouse isoform 2.

    Alternative splice isoforms for DCLK2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for DCLK2 Gene

Selected DME Specific Peptides for DCLK2 Gene

Q8N568:
  • GVITYILL
  • GPLYTVCGTPTYVAPEIIAETGYGLKVD
  • LGDFGLA
  • NEVSILRRVKHPNI
  • GNFAVVK
  • HRDIKPEN
  • LVMELVKGGDLFDAITS

Post-translational modifications for DCLK2 Gene

Domains & Families for DCLK2 Gene

Gene Families for DCLK2 Gene

HGNC:
IUPHAR :
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins

Suggested Antigen Peptide Sequences for DCLK2 Gene

GenScript: Design optimal peptide antigens:
  • Doublecortin-like kinase 2 (DCLK2_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q8N568

UniProtKB/Swiss-Prot:

DCLK2_HUMAN :
  • The doublecortin domains are involved in the colocalization with microtubules.
  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Domain:
  • The doublecortin domains are involved in the colocalization with microtubules.
Family:
  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
genes like me logo Genes that share domains with DCLK2: view

Function for DCLK2 Gene

Molecular function for DCLK2 Gene

UniProtKB/Swiss-Prot Function:
Protein kinase with a significantly reduced C(a2+)/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm (By similarity).
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1;.

Enzyme Numbers (IUBMB) for DCLK2 Gene

Phenotypes From GWAS Catalog for DCLK2 Gene

Gene Ontology (GO) - Molecular Function for DCLK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0004672 protein kinase activity IEA --
GO:0004674 protein serine/threonine kinase activity IEA --
GO:0005515 protein binding IPI --
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with DCLK2: view
genes like me logo Genes that share phenotypes with DCLK2: view

Animal Models for DCLK2 Gene

MGI Knock Outs for DCLK2:

Animal Models for research

  • Taconic Biosciences Mouse Models for DCLK2

miRNA for DCLK2 Gene

miRTarBase miRNAs that target DCLK2

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for DCLK2

Clone products for research

  • Addgene plasmids for DCLK2

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for DCLK2 Gene

Localization for DCLK2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for DCLK2 Gene

Cytoplasm, cytoskeleton. Note=Colocalizes with microtubules. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for DCLK2 gene
Compartment Confidence
cytoskeleton 5
cytosol 3
nucleus 2
plasma membrane 1
extracellular 1
mitochondrion 1
peroxisome 0

Gene Ontology (GO) - Cellular Components for DCLK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IEA --
GO:0005856 cytoskeleton IEA --
genes like me logo Genes that share ontologies with DCLK2: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for DCLK2 Gene

Pathways & Interactions for DCLK2 Gene

PathCards logo

SuperPathways for DCLK2 Gene

No Data Available

Gene Ontology (GO) - Biological Process for DCLK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000226 microtubule cytoskeleton organization IBA 21873635
GO:0006468 protein phosphorylation IEA --
GO:0016310 phosphorylation IEA --
GO:0035556 intracellular signal transduction IEA --
genes like me logo Genes that share ontologies with DCLK2: view

No data available for Pathways by source and SIGNOR curated interactions for DCLK2 Gene

Drugs & Compounds for DCLK2 Gene

(2) Drugs for DCLK2 Gene - From: DrugBank and HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
fostamatinib Approved, Investigational Pharma Target, inhibitor Kinase Inhibitors 0
ATP Investigational Nutra 0

(1) Additional Compounds for DCLK2 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • 5'-Adenylphosphoric acid
  • Adenosine 5'-diphosphate
  • ADENOSINE-5'-diphosphATE
  • H3ADP
  • 5'-Adenylphosphate
58-64-0
genes like me logo Genes that share compounds with DCLK2: view

Transcripts for DCLK2 Gene

mRNA/cDNA for DCLK2 Gene

3 REFSEQ mRNAs :
7 NCBI additional mRNA sequence :
7 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for DCLK2

Clone products for research

  • Addgene plasmids for DCLK2

Alternative Splicing Database (ASD) splice patterns (SP) for DCLK2 Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19 ^ 20
SP1: - - -
SP2: - - -
SP3: - - - -
SP4: - -

Relevant External Links for DCLK2 Gene

GeneLoc Exon Structure for
DCLK2

Expression for DCLK2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for DCLK2 Gene

Protein differential expression in normal tissues from HIPED for DCLK2 Gene

This gene is overexpressed in Fetal Brain (23.9), Brain (12.4), Frontal cortex (7.4), Cervix (6.9), and Retina (6.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for DCLK2 Gene



Protein tissue co-expression partners for DCLK2 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for DCLK2

SOURCE GeneReport for Unigene cluster for DCLK2 Gene:

Hs.591683

mRNA Expression by UniProt/SwissProt for DCLK2 Gene:

Q8N568-DCLK2_HUMAN
Tissue specificity: Expressed in the brain, heart and eyes.

Evidence on tissue expression from TISSUES for DCLK2 Gene

  • Nervous system(4.6)
genes like me logo Genes that share expression patterns with DCLK2: view

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for DCLK2 Gene

Orthologs for DCLK2 Gene

This gene was present in the common ancestor of animals.

Orthologs for DCLK2 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia DCLK2 29 30
  • 99.7 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia DCLK2 29 30
  • 93.26 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia DCLK2 30
  • 91 (a)
OneToOne
Cow
(Bos Taurus)
Mammalia DCLK2 29 30
  • 90.97 (n)
OneToOne
Mouse
(Mus musculus)
Mammalia Dclk2 29 16 30
  • 89.02 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Dclk2 29
  • 88.47 (n)
Platypus
(Ornithorhynchus anatinus)
Mammalia DCLK2 30
  • 85 (a)
OneToOne
Chicken
(Gallus gallus)
Aves DCLK2 29 30
  • 81.24 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia DCLK2 30
  • 78 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia LOC100487296 29
  • 75.48 (n)
Zebrafish
(Danio rerio)
Actinopterygii BX005047.1 30
  • 78 (a)
OneToMany
dclk2 29 30
  • 71.21 (n)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP009034 29
  • 53.71 (n)
Fruit Fly
(Drosophila melanogaster)
Insecta CG17528 29 30
  • 51.39 (n)
OneToMany
Worm
(Caenorhabditis elegans)
Secernentea zyg-8 30
  • 31 (a)
OneToMany
Species where no ortholog for DCLK2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for DCLK2 Gene

ENSEMBL:
Gene Tree for DCLK2 (if available)
TreeFam:
Gene Tree for DCLK2 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for DCLK2: view image
Alliance of Genome Resources:
Additional Orthologs for DCLK2

Paralogs for DCLK2 Gene

genes like me logo Genes that share paralogs with DCLK2: view

Variants for DCLK2 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for DCLK2 Gene

SNP ID Clinical significance and condition Chr 04 pos Variation AA Info Type
rs34386880 -- p.Arg372His
rs35745104 -- p.Ile583Val
rs56327537 -- p.Gly119Cys
rs759398144 -- p.Val212Met

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for DCLK2 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for DCLK2 Gene

Variant ID Type Subtype PubMed ID
dgv2918n106 CNV deletion 24896259
esv1001185 CNV insertion 20482838
esv2673603 CNV deletion 23128226
esv3569747 CNV loss 25503493
esv3602651 CNV loss 21293372
esv3602652 CNV loss 21293372
esv3602654 CNV loss 21293372
esv3894052 CNV gain 25118596
nsv1022132 CNV gain 25217958
nsv1074223 CNV deletion 25765185
nsv1123855 CNV deletion 24896259
nsv1149616 CNV insertion 26484159
nsv261 CNV insertion 15895083
nsv292617 CNV deletion 16902084
nsv4546 CNV insertion 18451855
nsv507204 OTHER sequence alteration 20534489
nsv509018 CNV insertion 20534489
nsv830111 CNV gain 17160897
nsv949829 CNV deletion 24416366
nsv949830 CNV deletion 24416366

Variation tolerance for DCLK2 Gene

Residual Variation Intolerance Score: 51.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.29; 40.97% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for DCLK2 Gene

Human Gene Mutation Database (HGMD)
DCLK2
SNPedia medical, phenotypic, and genealogical associations of SNPs for
DCLK2
Leiden Open Variation Database (LOVD)
DCLK2

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for DCLK2 Gene

Disorders for DCLK2 Gene

MalaCards: The human disease database

(3) MalaCards diseases for DCLK2 Gene - From: COP and GCD

- elite association - COSMIC cancer census association via MalaCards
Search DCLK2 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for DCLK2

genes like me logo Genes that share disorders with DCLK2: view

No data available for UniProtKB/Swiss-Prot and Genatlas for DCLK2 Gene

Publications for DCLK2 Gene

  1. Doublecortin-Like Kinases Promote Neuronal Survival and Induce Growth Cone Reformation via Distinct Mechanisms. (PMID: 26526391) Nawabi H … He Z (Neuron 2015) 2 3
  2. Doublecortin-like kinase enhances dendritic remodelling and negatively regulates synapse maturation. (PMID: 23385585) Shin E … Okabe S (Nature communications 2013) 2 3
  3. The doublecortin gene family and disorders of neuronal structure. (PMID: 20236041) Dijkmans TF … Vreugdenhil E (Central nervous system agents in medicinal chemistry 2010) 3 4
  4. Alternative transcripts of Dclk1 and Dclk2 and their expression in doublecortin knockout mice. (PMID: 18075264) Tuy FP … Francis F (Developmental neuroscience 2008) 3 4
  5. The evolving doublecortin (DCX) superfamily. (PMID: 16869982) Reiner O … Bergmann S (BMC genomics 2006) 3 4

Products for DCLK2 Gene

Sources for DCLK2 Gene