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Aliases for DAPK2 Gene

Aliases for DAPK2 Gene

  • Death Associated Protein Kinase 2 2 3 5
  • DAP Kinase 2 3 4
  • DRP-1 3 4
  • DAP-Kinase-Related Protein 1 Beta Isoform 3
  • Death-Associated Protein Kinase 2 3
  • DAP-Kinase-Related Protein 1 4
  • EC 2.7.11.1 4
  • DRP1 3

External Ids for DAPK2 Gene

Previous GeneCards Identifiers for DAPK2 Gene

  • GC15M059986
  • GC15M057358
  • GC15M061778
  • GC15M061915
  • GC15M061986
  • GC15M064199
  • GC15M041023

Summaries for DAPK2 Gene

Entrez Gene Summary for DAPK2 Gene

  • This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq, Jul 2008]

GeneCards Summary for DAPK2 Gene

DAPK2 (Death Associated Protein Kinase 2) is a Protein Coding gene. Among its related pathways are Bladder cancer and Apoptosis Modulation and Signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and protein kinase activity. An important paralog of this gene is DAPK1.

UniProtKB/Swiss-Prot for DAPK2 Gene

  • Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell death signals, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation (PubMed:17347302). Regulates granulocytic motility by controlling cell spreading and polarization (PubMed:24163421).

  • Isoform 2 is not regulated by calmodulin. It can phosphorylate MYL9. It can induce membrane blebbing and autophagic cell death.

Gene Wiki entry for DAPK2 Gene

Additional gene information for DAPK2 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for DAPK2 Gene

Genomics for DAPK2 Gene

GeneHancer (GH) Regulatory Elements for DAPK2 Gene

Promoters and enhancers for DAPK2 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH15J064044 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE dbSUPER 657.4 +26.4 26409 2.9 HDGF RB1 SIN3A IRF4 RAD21 ZNF335 GLIS2 GATA2 ZNF143 BCLAF1 DAPK2 LOC101928988
GH15J064069 Enhancer 1.4 Ensembl ENCODE dbSUPER 650.7 +0.6 600 4.1 FOXA2 MLX ARID4B YY1 SLC30A9 ZNF766 ZNF143 FOS SP5 REST DAPK2 TRIP4 ZNF609 SNX1 ENSG00000264937 CIAO2A SNX22 ENSG00000265967 HERC1 USP3
GH15J063891 Enhancer 1.8 FANTOM5 Ensembl ENCODE dbSUPER 26.2 +177.5 177528 6.8 HDGF PKNOX1 CLOCK FOXA2 ARNT NEUROD1 SIN3A ZNF2 IRF4 YY1 DAPK2 TRIP4 HERC1 CSNK1G1 USP3 MIR422A SNX1 TPM1-AS USP3-AS1 RPS27L
GH15J063984 Enhancer 1.4 FANTOM5 Ensembl ENCODE dbSUPER 32.5 +85.4 85353 3.7 HDAC1 PKNOX1 STAT5A BATF IRF4 ATF7 ETV6 BCLAF1 IKZF2 RUNX3 DAPK2 HERC1 MIR422A USP3 TRIP4 LOC101928988
GH15J063912 Enhancer 1.2 Ensembl ENCODE dbSUPER 29.6 +157.1 157058 4.3 KLF17 SIN3A RAD21 ZNF366 SCRT2 ATF7 RUNX3 EGR2 SP3 JUNB DAPK2 CSNK1G1 HERC1 TRIP4 LOC101928988 ENSG00000265967 SNX1 TPM1-AS SNX22 TPM1
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around DAPK2 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the DAPK2 gene promoter:

Genomic Locations for DAPK2 Gene

Genomic Locations for DAPK2 Gene
chr15:63,907,036-64,072,033
(GRCh38/hg38)
Size:
164,998 bases
Orientation:
Minus strand
chr15:64,199,235-64,364,232
(GRCh37/hg19)

Genomic View for DAPK2 Gene

Genes around DAPK2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
DAPK2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for DAPK2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for DAPK2 Gene

Proteins for DAPK2 Gene

  • Protein details for DAPK2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9UIK4-DAPK2_HUMAN
    Recommended name:
    Death-associated protein kinase 2
    Protein Accession:
    Q9UIK4
    Secondary Accessions:
    • E9JGM7
    • O75892
    • Q24JS1

    Protein attributes for DAPK2 Gene

    Size:
    370 amino acids
    Molecular mass:
    42898 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Homodimer in its autoinhibited state. Active as monomer (By similarity). Isoform 2 but not isoform 1 can interact with ATF4. Interacts with 14-3-3 proteins YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ, YWHAZ and SFN; the interaction requires DAPK2 phosphorylation at Thr-369 and suppresses DAPK2 kinase activity and DAPK2-induced apoptosis (PubMed:26047703).

    Three dimensional structures from OCA and Proteopedia for DAPK2 Gene

    Alternative splice isoforms for DAPK2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for DAPK2 Gene

Post-translational modifications for DAPK2 Gene

  • Autophosphorylation at Ser-318 inhibits its catalytic activity. Dephosphorylated at Ser-318 in response to activated Fas and TNF-alpha receptors.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for DAPK2 Gene

Domains & Families for DAPK2 Gene

Gene Families for DAPK2 Gene

HGNC:
IUPHAR :
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins
  • Predicted membrane proteins

Protein Domains for DAPK2 Gene

Suggested Antigen Peptide Sequences for DAPK2 Gene

Graphical View of Domain Structure for InterPro Entry

Q9UIK4

UniProtKB/Swiss-Prot:

DAPK2_HUMAN :
  • The autoinhibitory domain sterically blocks the substrate peptide-binding site by making both hydrophobic and electrostatic contacts with the kinase core.
  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Domain:
  • The autoinhibitory domain sterically blocks the substrate peptide-binding site by making both hydrophobic and electrostatic contacts with the kinase core.
Family:
  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
genes like me logo Genes that share domains with DAPK2: view

Function for DAPK2 Gene

Molecular function for DAPK2 Gene

UniProtKB/Swiss-Prot Function:
Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell death signals, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation (PubMed:17347302). Regulates granulocytic motility by controlling cell spreading and polarization (PubMed:24163421).
UniProtKB/Swiss-Prot Function:
Isoform 2 is not regulated by calmodulin. It can phosphorylate MYL9. It can induce membrane blebbing and autophagic cell death.
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
Activated by Ca(2+)/calmodulin. Regulated by a double locking mechanism, involving autophosphorylation at Ser-318, calmodulin binding, and dimerization. In the inactive state, Ser-318 is phosphorylated, and the kinase is dimeric. Activation involves: dephosphorylation at Ser-318, release-of-autoinhibition mechanism where calmodulin binding induces a conformational change that relieves the steric block of the active site by the autoinhibitory domain, and generation of the monomeric active form of the kinase.
UniProtKB/Swiss-Prot Induction:
Up-regulated during granulocytic maturation (PubMed:17347302, PubMed:24163421).

Enzyme Numbers (IUBMB) for DAPK2 Gene

Phenotypes From GWAS Catalog for DAPK2 Gene

Gene Ontology (GO) - Molecular Function for DAPK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity IEA --
GO:0004674 protein serine/threonine kinase activity TAS 16756490
GO:0005515 protein binding IPI 25416956
GO:0005516 calmodulin binding IDA,IEA 10376525
GO:0005524 ATP binding IDA 10376525
genes like me logo Genes that share ontologies with DAPK2: view
genes like me logo Genes that share phenotypes with DAPK2: view

Animal Models for DAPK2 Gene

MGI Knock Outs for DAPK2:
  • Dapk2 Dapk2<tm1b(EUCOMM)Wtsi>

Animal Model Products

  • Taconic Biosciences Mouse Models for DAPK2

Clone Products

  • Addgene plasmids for DAPK2

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for DAPK2 Gene

Localization for DAPK2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for DAPK2 Gene

Cytoplasm. Cytoplasmic vesicle, autophagosome lumen.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for DAPK2 gene
Compartment Confidence
golgi apparatus 4
cytosol 3
nucleus 2
mitochondrion 1
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Vesicles (3)
  • Golgi apparatus (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for DAPK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IDA,IEA 10376525
GO:0005794 Golgi apparatus IDA --
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane IEA --
GO:0031410 cytoplasmic vesicle IEA --
genes like me logo Genes that share ontologies with DAPK2: view

Pathways & Interactions for DAPK2 Gene

genes like me logo Genes that share pathways with DAPK2: view

Pathways by source for DAPK2 Gene

Gene Ontology (GO) - Biological Process for DAPK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006468 protein phosphorylation IDA 10376525
GO:0006915 apoptotic process TAS 10629061
GO:0010506 regulation of autophagy TAS 16756490
GO:0016310 phosphorylation IEA --
GO:0018105 peptidyl-serine phosphorylation IBA --
genes like me logo Genes that share ontologies with DAPK2: view

No data available for SIGNOR curated interactions for DAPK2 Gene

Drugs & Compounds for DAPK2 Gene

(7) Drugs for DAPK2 Gene - From: DrugBank, ApexBio, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
fostamatinib Approved, Investigational Pharma Target, inhibitor Kinase Inhibitors 0
calcium Approved Nutra 0
Magnesium Approved Nutra 0
ATP Investigational Nutra Agonist, Activator, Full agonist, Antagonist, Potentiation, Pore Blocker 0
DAPK Substrate Peptide Pharma Synthetic peptide substrate for DAPK, Death associated protein kinase substrate (synthetic) 0

(1) Additional Compounds for DAPK2 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • 5'-Adenylphosphoric acid
  • Adenosine 5'-diphosphate
  • ADENOSINE-5'-diphosphATE
  • H3ADP
  • 5'-Adenylphosphate
Full agonist, Agonist, Partial agonist, Antagonist, Gating inhibitor 58-64-0

(1) ApexBio Compounds for DAPK2 Gene

Compound Action Cas Number
DAPK Substrate Peptide Synthetic peptide substrate for DAPK 386769-53-5
genes like me logo Genes that share compounds with DAPK2: view

Drug Products

Transcripts for DAPK2 Gene

Unigene Clusters for DAPK2 Gene

Death-associated protein kinase 2:
Representative Sequences:

Clone Products

  • Addgene plasmids for DAPK2

Alternative Splicing Database (ASD) splice patterns (SP) for DAPK2 Gene

No ASD Table

Relevant External Links for DAPK2 Gene

GeneLoc Exon Structure for
DAPK2
ECgene alternative splicing isoforms for
DAPK2

Expression for DAPK2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for DAPK2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for DAPK2 Gene

This gene is overexpressed in Whole Blood (x6.7), Heart - Atrial Appendage (x5.2), Muscle - Skeletal (x4.9), and Thyroid (x4.7).

Protein differential expression in normal tissues from HIPED for DAPK2 Gene

This gene is overexpressed in Heart (47.3) and Testis (21.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for DAPK2 Gene



Protein tissue co-expression partners for DAPK2 Gene

NURSA nuclear receptor signaling pathways regulating expression of DAPK2 Gene:

DAPK2

SOURCE GeneReport for Unigene cluster for DAPK2 Gene:

Hs.237886

mRNA Expression by UniProt/SwissProt for DAPK2 Gene:

Q9UIK4-DAPK2_HUMAN
Tissue specificity: Expressed in neutrophils and eosinophils (PubMed:24163421). Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle.
genes like me logo Genes that share expression patterns with DAPK2: view

No data available for Evidence on tissue expression from TISSUES and Phenotype-based relationships between genes and organs from Gene ORGANizer for DAPK2 Gene

Orthologs for DAPK2 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for DAPK2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia DAPK2 34
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia DAPK2 34 33
  • 93.24 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Dapk2 16 34 33
  • 91.8 (n)
dog
(Canis familiaris)
Mammalia DAPK2 34
  • 63 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia DAPK2 34
  • 60 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia DAPK2 34
  • 49 (a)
OneToOne
chicken
(Gallus gallus)
Aves DAPK2 34
  • 54 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia -- 34
  • 57 (a)
ManyToMany
tropical clawed frog
(Silurana tropicalis)
Amphibia dapk2 33
  • 73.61 (n)
zebrafish
(Danio rerio)
Actinopterygii im:7162965 33
  • 72.58 (n)
DAPK2 (1 of 2) 34
  • 65 (a)
OneToMany
dapk2 34
  • 49 (a)
OneToMany
fruit fly
(Drosophila melanogaster)
Insecta bt 34
  • 2 (a)
ManyToMany
worm
(Caenorhabditis elegans)
Secernentea dapk-1 34
  • 11 (a)
OneToMany
thale cress
(Arabidopsis thaliana)
eudicotyledons PEPKR2 33
  • 47.66 (n)
rice
(Oryza sativa)
Liliopsida Os09g0466900 33
  • 47.34 (n)
sea squirt
(Ciona savignyi)
Ascidiacea -- 34
  • 42 (a)
OneToMany
Species where no ortholog for DAPK2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rat (Rattus norvegicus)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for DAPK2 Gene

ENSEMBL:
Gene Tree for DAPK2 (if available)
TreeFam:
Gene Tree for DAPK2 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for DAPK2: view image

Paralogs for DAPK2 Gene

Paralogs for DAPK2 Gene

genes like me logo Genes that share paralogs with DAPK2: view

Variants for DAPK2 Gene

Sequence variations from dbSNP and Humsavar for DAPK2 Gene

SNP ID Clin Chr 15 pos Variation AA Info Type
rs1000107348 -- 63,935,552(-) C/T genic_upstream_transcript_variant, intron_variant
rs1000145543 -- 63,918,191(-) C/G/T coding_sequence_variant, genic_downstream_transcript_variant, intron_variant, missense_variant
rs1000151449 -- 63,926,045(-) C/T coding_sequence_variant, synonymous_variant
rs1000159768 -- 63,957,927(-) G/A genic_upstream_transcript_variant, intron_variant
rs1000168350 -- 64,032,856(-) T/C genic_upstream_transcript_variant, intron_variant

Structural Variations from Database of Genomic Variants (DGV) for DAPK2 Gene

Variant ID Type Subtype PubMed ID
esv1414761 CNV insertion 17803354
esv3636720 CNV loss 21293372
esv3636721 CNV gain 21293372
esv3636725 OTHER inversion 21293372
esv3636727 CNV loss 21293372
nsv1134458 CNV deletion 24896259
nsv1567 CNV deletion 18451855
nsv1568 CNV insertion 18451855
nsv1569 CNV deletion 18451855
nsv475825 CNV novel sequence insertion 20440878

Variation tolerance for DAPK2 Gene

Residual Variation Intolerance Score: 72.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 12.68; 94.61% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for DAPK2 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
DAPK2

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for DAPK2 Gene

Disorders for DAPK2 Gene

Additional Disease Information for DAPK2

No disorders were found for DAPK2 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for DAPK2 Gene

Publications for DAPK2 Gene

  1. DAP kinase and DRP-1 mediate membrane blebbing and the formation of autophagic vesicles during programmed cell death. (PMID: 11980920) Inbal B … Kimchi A (The Journal of cell biology 2002) 3 4 22 58
  2. Autophosphorylation restrains the apoptotic activity of DRP-1 kinase by controlling dimerization and calmodulin binding. (PMID: 11230133) Shani G … Kimchi A (The EMBO journal 2001) 3 4 22 58
  3. Death-associated protein kinase-related protein 1, a novel serine/threonine kinase involved in apoptosis. (PMID: 10629061) Inbal B … Kimchi A (Molecular and cellular biology 2000) 3 4 22 58
  4. Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity. (PMID: 10376525) Kawai T … Akira S (Oncogene 1999) 2 3 4 58
  5. Suppression of death-associated protein kinase 2 by interaction with 14-3-3 proteins. (PMID: 26047703) Yuasa K … Tsuji A (Biochemical and biophysical research communications 2015) 3 4 58

Products for DAPK2 Gene

  • Addgene plasmids for DAPK2

Sources for DAPK2 Gene

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