DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly as... See more...

Aliases for DAD1 Gene

Aliases for DAD1 Gene

  • Defender Against Cell Death 1 2 3 4 5
  • Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit DAD1 2 3 4
  • Oligosaccharyltransferase Subunit 2 (Non-Catalytic) 2 3
  • Oligosaccharyl Transferase Subunit DAD1 3 4
  • DAD-1 3 4
  • OST2 2 3
  • Oligosaccharyltransferase 2 Homolog (S. Cerevisiae) 2
  • Oligosaccharyltransferase 2 Homolog 3
  • EC 2.4.1.119 50
  • DAD1 5

External Ids for DAD1 Gene

Previous GeneCards Identifiers for DAD1 Gene

  • GC14M020406
  • GC14M016821
  • GC14M021023
  • GC14M022103
  • GC14M023033
  • GC14M003152

Summaries for DAD1 Gene

Entrez Gene Summary for DAD1 Gene

  • DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008]

GeneCards Summary for DAD1 Gene

DAD1 (Defender Against Cell Death 1) is a Protein Coding gene. Diseases associated with DAD1 include Ogden Syndrome and Granulomatous Disease, Chronic, Autosomal Recessive, 1. Among its related pathways are Protein processing in endoplasmic reticulum and N-Glycan biosynthesis. Gene Ontology (GO) annotations related to this gene include dolichyl-diphosphooligosaccharide-protein glycotransferase activity and oligosaccharyl transferase activity.

UniProtKB/Swiss-Prot Summary for DAD1 Gene

  • Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:22467853). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity). Required for the assembly of both SST3A- and SS3B-containing OST complexes. Loss of the DAD1 protein triggers apoptosis (PubMed:22467853).

Gene Wiki entry for DAD1 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for DAD1 Gene

Genomics for DAD1 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for DAD1 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around DAD1 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for DAD1

Top Transcription factor binding sites by QIAGEN in the DAD1 gene promoter:
  • CREB
  • deltaCREB
  • GCNF-2
  • p53
  • Pax-2
  • Pax-2a
  • Pax-2b
  • POU3F2
  • Sp1
  • TBP

Genomic Locations for DAD1 Gene

Latest Assembly
chr14:22,564,907-22,589,224
(GRCh38/hg38)
Size:
24,318 bases
Orientation:
Minus strand

Previous Assembly
chr14:23,033,807-23,058,130
(GRCh37/hg19 by Entrez Gene)
Size:
24,324 bases
Orientation:
Minus strand

chr14:23,033,805-23,058,175
(GRCh37/hg19 by Ensembl)
Size:
24,371 bases
Orientation:
Minus strand

Genomic View for DAD1 Gene

Genes around DAD1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
DAD1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for DAD1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for DAD1 Gene

Proteins for DAD1 Gene

  • Protein details for DAD1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P61803-DAD1_HUMAN
    Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
    Protein Accession:
    P61803
    Secondary Accessions:
    • D3DS25
    • O08552
    • O70364
    • P46966
    • P46968
    • Q6FGA3
    • Q96GB7

    Protein attributes for DAD1 Gene

    Size:
    113 amino acids
    Molecular mass:
    12497 Da
    Quaternary structure:
    • Component of the oligosaccharyltransferase (OST) complex (PubMed:25135935). OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. STT3A complex assembly occurs through the formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258, subcomplex 2 contains the STT3A-specific subunits STT3A, DC2/OSTC, and KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2, DAD1, and OST48. The STT3A complex can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes (By similarity).

    Three dimensional structures from OCA and Proteopedia for DAD1 Gene

neXtProt entry for DAD1 Gene

Selected DME Specific Peptides for DAD1 Gene

P61803:
  • YCLLVGTFPFNSFLSGFIS
  • SVISRFLEEYLS
  • FQGISPERAFADFLFA
  • LTGALQFGYCL

Post-translational modifications for DAD1 Gene

  • Ubiquitination at Lys82
  • Modification sites at PhosphoSitePlus

Other Protein References for DAD1 Gene

Antibodies for research

  • Abcam antibodies for DAD1

Domains & Families for DAD1 Gene

Gene Families for DAD1 Gene

HGNC:
Human Protein Atlas (HPA):
  • Cancer-related genes
  • Predicted membrane proteins

Protein Domains for DAD1 Gene

InterPro:

Suggested Antigen Peptide Sequences for DAD1 Gene

GenScript: Design optimal peptide antigens:
  • Defender against cell death 1 (DAD1_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P61803

UniProtKB/Swiss-Prot:

DAD1_HUMAN :
  • Belongs to the DAD/OST2 family.
Family:
  • Belongs to the DAD/OST2 family.
genes like me logo Genes that share domains with DAD1: view

Function for DAD1 Gene

Molecular function for DAD1 Gene

UniProtKB/Swiss-Prot Function:
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:22467853). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity). Required for the assembly of both SST3A- and SS3B-containing OST complexes. Loss of the DAD1 protein triggers apoptosis (PubMed:22467853).
GENATLAS Biochemistry:
defender against apoptosis

Enzyme Numbers (IUBMB) for DAD1 Gene

Phenotypes From GWAS Catalog for DAD1 Gene

Gene Ontology (GO) - Molecular Function for DAD1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0008047 enzyme activator activity IMP 22467853
genes like me logo Genes that share ontologies with DAD1: view
genes like me logo Genes that share phenotypes with DAD1: view

Animal Models for DAD1 Gene

MGI Knock Outs for DAD1:

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for DAD1

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for DAD1 Gene

Localization for DAD1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for DAD1 Gene

Endoplasmic reticulum membrane. Multi-pass membrane protein.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for DAD1 gene
Compartment Confidence
endoplasmic reticulum 4
plasma membrane 3
extracellular 2
nucleus 2
cytoskeleton 1
mitochondrion 1
endosome 1
cytosol 1
lysosome 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for DAD1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005783 endoplasmic reticulum IEA --
GO:0005789 endoplasmic reticulum membrane IEA,TAS --
GO:0008250 oligosaccharyltransferase complex IEA,TAS 15835887
GO:0016020 membrane IEA,HDA 19946888
GO:0016021 integral component of membrane IEA --
genes like me logo Genes that share ontologies with DAD1: view

Pathways & Interactions for DAD1 Gene

genes like me logo Genes that share pathways with DAD1: view

Pathways by source for DAD1 Gene

1 GeneGo (Thomson Reuters) pathway for DAD1 Gene
  • CFTR folding and maturation (norm and CF)

UniProtKB/Swiss-Prot P61803-DAD1_HUMAN

  • Pathway: Protein modification; protein glycosylation.

Gene Ontology (GO) - Biological Process for DAD1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001824 blastocyst development IEA --
GO:0006486 protein glycosylation IEA,ISS --
GO:0006487 protein N-linked glycosylation IMP 22467853
GO:0006915 apoptotic process IEA --
GO:0007584 response to nutrient IEA --
genes like me logo Genes that share ontologies with DAD1: view

No data available for SIGNOR curated interactions for DAD1 Gene

Drugs & Compounds for DAD1 Gene

(1) Drugs for DAD1 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Asparagine Approved, Investigational Nutra 40

(1) Additional Compounds for DAD1 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Dolichyl diphosphate
  • Dolichol diphosphate
  • Dolichol diphosphoric acid
  • Dolichyl diphosphoric acid
  • Dolichol pyrophosphate
  • Dolichyl pyrophosphate
37247-98-6
genes like me logo Genes that share compounds with DAD1: view

Transcripts for DAD1 Gene

mRNA/cDNA for DAD1 Gene

1 REFSEQ mRNAs :
8 NCBI additional mRNA sequence :
5 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for DAD1

Alternative Splicing Database (ASD) splice patterns (SP) for DAD1 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b ^ 3 ^ 4 ^ 5a · 5b · 5c · 5d
SP1: -
SP2: - -
SP3: - - -
SP4:
SP5: - - - -

Relevant External Links for DAD1 Gene

GeneLoc Exon Structure for
DAD1

Expression for DAD1 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for DAD1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for DAD1 Gene

This gene is overexpressed in Nasal epithelium (32.5).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for DAD1 Gene



Protein tissue co-expression partners for DAD1 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for DAD1

SOURCE GeneReport for Unigene cluster for DAD1 Gene:

Hs.82890

Evidence on tissue expression from TISSUES for DAD1 Gene

  • Nervous system(4.7)
  • Skin(4.5)
  • Blood(4.5)
  • Kidney(2.8)
  • Liver(2.7)
  • Muscle(2.6)
  • Intestine(2.6)
  • Lymph node(2.6)
  • Heart(2.5)
  • Spleen(2.4)
  • Lung(2.3)
  • Pancreas(2.3)
  • Bone marrow(2.2)
  • Thyroid gland(2.2)
  • Adrenal gland(2.1)
  • Gall bladder(2.1)
genes like me logo Genes that share expression patterns with DAD1: view

No data available for mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for DAD1 Gene

Orthologs for DAD1 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for DAD1 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia DAD1 29 30
  • 99.71 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia DAD1 29 30
  • 93.51 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia DAD1 29 30
  • 92.33 (n)
OneToOne
Mouse
(Mus musculus)
Mammalia Dad1 29 16 30
  • 91.45 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Dad1 29
  • 90.27 (n)
Oppossum
(Monodelphis domestica)
Mammalia DAD1 30
  • 77 (a)
OneToOne
Chicken
(Gallus gallus)
Aves DAD1 29 30
  • 74.7 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia DAD1 30
  • 75 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia dad1 29
  • 75.52 (n)
Str.10052 29
African clawed frog
(Xenopus laevis)
Amphibia LOC397721 29
Zebrafish
(Danio rerio)
Actinopterygii dad1 29 30
  • 70.5 (n)
OneToOne
Fruit Fly
(Drosophila melanogaster)
Insecta CG13393 31
  • 71 (a)
l(2)k12914 29 30
  • 63.55 (n)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP008491 29
  • 63.06 (n)
Worm
(Caenorhabditis elegans)
Secernentea dad-1 29 30 31
  • 61.65 (n)
OneToOne
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes OST2 30 32
  • 34 (a)
OneToOne
Thale Cress
(Arabidopsis thaliana)
eudicotyledons ATDAD1 29
  • 55.56 (n)
Rice
(Oryza sativa)
Liliopsida Os04g0397000 29
  • 56.67 (n)
Sea Squirt
(Ciona savignyi)
Ascidiacea CSA.5446 30
  • 63 (a)
OneToOne
Species where no ortholog for DAD1 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Platypus (Ornithorhynchus anatinus)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for DAD1 Gene

ENSEMBL:
Gene Tree for DAD1 (if available)
TreeFam:
Gene Tree for DAD1 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for DAD1: view image
Alliance of Genome Resources:
Additional Orthologs for DAD1

Paralogs for DAD1 Gene

Pseudogenes.org Pseudogenes for DAD1 Gene

genes like me logo Genes that share paralogs with DAD1: view

No data available for Paralogs for DAD1 Gene

Variants for DAD1 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for DAD1 Gene

SNP ID Clinical significance and condition Chr 14 pos Variation AA Info Type
rs5742796 -- p.Ala83Thr

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for DAD1 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for DAD1 Gene

Variant ID Type Subtype PubMed ID
esv2678511 CNV deletion 23128226
esv3633757 CNV loss 21293372
esv3892469 CNV gain+loss 25118596
nsv1047315 CNV gain 25217958
nsv519534 CNV loss 19592680
nsv563998 CNV gain 21841781
nsv832746 CNV gain 17160897
nsv832748 CNV gain 17160897

Variation tolerance for DAD1 Gene

Residual Variation Intolerance Score: 48.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.15; 3.33% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for DAD1 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
DAD1
Leiden Open Variation Database (LOVD)
DAD1

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for DAD1 Gene

Disorders for DAD1 Gene

MalaCards: The human disease database

(4) MalaCards diseases for DAD1 Gene - From: COP and GCD

- elite association - COSMIC cancer census association via MalaCards
Search DAD1 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for DAD1

Human Genome Epidemiology Navigator
(HuGE)
Atlas
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
Open Targets Platform
genes like me logo Genes that share disorders with DAD1: view

No data available for UniProtKB/Swiss-Prot and Genatlas for DAD1 Gene

Publications for DAD1 Gene

  1. Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells. (PMID: 8413235) Nakashima T … Nishimoto T (Molecular and cellular biology 1993) 3 4 22
  2. The oligosaccharyltransferase subunits OST48, DAD1 and KCP2 function as ubiquitous and selective modulators of mammalian N-glycosylation. (PMID: 22467853) Roboti P … High S (Journal of cell science 2012) 3 4
  3. A Large-scale genetic association study of esophageal adenocarcinoma risk. (PMID: 20453000) Liu CY … Christiani DC (Carcinogenesis 2010) 3 40
  4. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PMID: 20379614) Rose JE … Uhl GR (Molecular medicine (Cambridge, Mass.) 2010) 3 40
  5. An approach based on a genome-wide association study reveals candidate loci for narcolepsy. (PMID: 20677014) Shimada M … Tokunaga K (Human genetics 2010) 3 40

Products for DAD1 Gene

Sources for DAD1 Gene