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Aliases for CPA4 Gene

Aliases for CPA4 Gene

  • Carboxypeptidase A4 2 3 3 5
  • Carboxypeptidase A3 2 3 4
  • CPA3 3 4
  • EC 3.4.17.- 4

External Ids for CPA4 Gene

Previous GeneCards Identifiers for CPA4 Gene

  • GC07P128409
  • GC07P129472
  • GC07P129485
  • GC07P129526
  • GC07P129720
  • GC07P129932
  • GC07P124295

Summaries for CPA4 Gene

Entrez Gene Summary for CPA4 Gene

  • This gene is a member of the carboxypeptidase A/B subfamily, and it is located in a cluster with three other family members on chromosome 7. Carboxypeptidases are zinc-containing exopeptidases that catalyze the release of carboxy-terminal amino acids, and are synthesized as zymogens that are activated by proteolytic cleavage. This gene could be involved in the histone hyperacetylation pathway. It is imprinted and may be a strong candidate gene for prostate cancer aggressiveness. [provided by RefSeq, Jul 2008]

GeneCards Summary for CPA4 Gene

CPA4 (Carboxypeptidase A4) is a Protein Coding gene. Diseases associated with CPA4 include Richter's Syndrome and Silver-Russell Syndrome. Gene Ontology (GO) annotations related to this gene include metallocarboxypeptidase activity and carboxypeptidase activity. An important paralog of this gene is CPA2.

UniProtKB/Swiss-Prot for CPA4 Gene

  • Metalloprotease that could be involved in the histone hyperacetylation pathway (PubMed:10383164). Releases a C-terminal amino acid, with preference for -Phe, -Leu, -Ile, -Met, -Tyr and -Val (PubMed:20385563).

Gene Wiki entry for CPA4 Gene

Additional gene information for CPA4 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CPA4 Gene

Genomics for CPA4 Gene

GeneHancer (GH) Regulatory Elements for CPA4 Gene

Promoters and enhancers for CPA4 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH07J130290 Promoter/Enhancer 1.5 EPDnew Ensembl ENCODE 662.5 -0.2 -239 5 PKNOX1 RAD21 BRCA1 RFX5 ZNF366 RCOR1 FOS DEK EGR2 CEBPB CPA4 CPA5 CPA2 GC07M130268
GH07J130440 Promoter/Enhancer 2 EPDnew Ensembl ENCODE 10.2 +148.7 148730 3.4 PKNOX1 ATF1 ARID4B SIN3A FEZF1 GLI4 ETS1 ZNF121 GLIS2 E2F8 CEP41 LOC105375505 MEST CPA4 GC07M130398
GH07J130391 Enhancer 1.2 Ensembl ENCODE dbSUPER 15 +103.6 103630 10.1 ARNT TCF12 ATF7 ZEB2 ELF1 ATF4 SMARCA4 IKZF1 NBN PRDM1 MEST CPA4 CPA5 CEP41 GC07M130398 CPA1
GH07J130354 Enhancer 1.4 Ensembl ENCODE dbSUPER 10.6 +64.4 64426 5.7 PKNOX1 ZNF133 SIN3A FEZF1 GLI4 ZNF2 FOS KLF13 RXRA YY2 CPA5 MEST NRF1 CPA1 KLHDC10 CPA4 CPA2 COPG2 LOC105375504
GH07J130344 Promoter/Enhancer 1.3 EPDnew Ensembl ENCODE 11.4 +52.3 52313 2.5 BCOR ZNF263 ATF1 NRF1 ZMYM3 ZIC2 ATF2 POLR2A ZNF341 CREB1 CPA5 LOC105375503 NRF1 CPA1 CPA4 LOC105375504
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around CPA4 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the CPA4 gene promoter:
  • E2F-1
  • E2F
  • NF-Y
  • E2F-2
  • E2F-3a
  • E2F-4
  • E2F-5
  • LCR-F1
  • CBF(2)

Genomic Locations for CPA4 Gene

Genomic Locations for CPA4 Gene
31,047 bases
Plus strand
31,047 bases
Plus strand

Genomic View for CPA4 Gene

Genes around CPA4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CPA4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CPA4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CPA4 Gene

Proteins for CPA4 Gene

  • Protein details for CPA4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Carboxypeptidase A4
    Protein Accession:
    Secondary Accessions:
    • B7Z576
    • Q86UY9

    Protein attributes for CPA4 Gene

    421 amino acids
    Molecular mass:
    47351 Da
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
    Quaternary structure:
    • Monomer. Interacts with LXN.

    Three dimensional structures from OCA and Proteopedia for CPA4 Gene

    Alternative splice isoforms for CPA4 Gene


neXtProt entry for CPA4 Gene

Post-translational modifications for CPA4 Gene

  • Glycosylation at Asn260
  • Modification sites at PhosphoSitePlus

Other Protein References for CPA4 Gene

No data available for DME Specific Peptides for CPA4 Gene

Domains & Families for CPA4 Gene

Gene Families for CPA4 Gene

Human Protein Atlas (HPA):
  • Enzymes
  • Plasma proteins
  • Predicted intracellular proteins
  • Predicted secreted proteins

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the peptidase M14 family.
  • Belongs to the peptidase M14 family.
genes like me logo Genes that share domains with CPA4: view

Function for CPA4 Gene

Molecular function for CPA4 Gene

UniProtKB/Swiss-Prot Function:
Metalloprotease that could be involved in the histone hyperacetylation pathway (PubMed:10383164). Releases a C-terminal amino acid, with preference for -Phe, -Leu, -Ile, -Met, -Tyr and -Val (PubMed:20385563).
UniProtKB/Swiss-Prot EnzymeRegulation:
Inhibited by interaction with the metallocarboxypeptidase inhibitor (MCPI) from N.versicolor that binds to the catalytic zinc ion.
UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=55.6 uM for 3-(2-furyl)acryloyl-Phe-Phe {ECO:0000269 PubMed:20385563}; KM=19.4 uM for 3-(2-furyl)acryloyl-Phe-Leu {ECO:0000269 PubMed:20385563}; KM=23.3 uM for 3-(2-furyl)acryloyl-Phe-Ile {ECO:0000269 PubMed:20385563}; KM=40.4 uM for 3-(2-furyl)acryloyl-Phe-Met {ECO:0000269 PubMed:20385563}; KM=57.3 uM for 3-(2-furyl)acryloyl-Phe-Val {ECO:0000269 PubMed:20385563}; KM=0.329 uM for neurotensin {ECO:0000269 PubMed:20385563}; KM=9.23 uM for Met-enkephalin-Arg-Phe {ECO:0000269 PubMed:20385563}; pH dependence: Optimum pH is 8.5-9. {ECO:0000269 PubMed:20385563};
UniProtKB/Swiss-Prot Induction:
Up-regulated by inhibitors of histone dacetylation.

Enzyme Numbers (IUBMB) for CPA4 Gene

Phenotypes From GWAS Catalog for CPA4 Gene

Gene Ontology (GO) - Molecular Function for CPA4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004181 metallocarboxypeptidase activity NAS 10383164
GO:0008233 peptidase activity IEA --
GO:0008237 metallopeptidase activity IEA --
GO:0008270 zinc ion binding IEA --
GO:0016787 hydrolase activity IEA --
genes like me logo Genes that share ontologies with CPA4: view
genes like me logo Genes that share phenotypes with CPA4: view

Animal Model Products

  • Taconic Biosciences Mouse Models for CPA4

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CPA4

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for CPA4 Gene

Localization for CPA4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CPA4 Gene


Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CPA4 gene
Compartment Confidence
extracellular 5
cytoskeleton 2
nucleus 2
cytosol 2
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Centrosome (2)
  • Cytosol (2)
  • Nucleoplasm (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for CPA4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005575 cellular_component ND --
GO:0005576 extracellular region IEA --
GO:0005615 extracellular space IBA --
genes like me logo Genes that share ontologies with CPA4: view

Pathways & Interactions for CPA4 Gene

No Data Available

Gene Ontology (GO) - Biological Process for CPA4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006508 proteolysis IEA --
GO:0016573 histone acetylation NAS 10383164
genes like me logo Genes that share ontologies with CPA4: view

No data available for Pathways by source and SIGNOR curated interactions for CPA4 Gene

Drugs & Compounds for CPA4 Gene

No Compound Related Data Available

Transcripts for CPA4 Gene

mRNA/cDNA for CPA4 Gene

Unigene Clusters for CPA4 Gene

Carboxypeptidase A4:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CPA4

Alternative Splicing Database (ASD) splice patterns (SP) for CPA4 Gene

No ASD Table

Relevant External Links for CPA4 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for CPA4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for CPA4 Gene

mRNA differential expression in normal tissues according to GTEx for CPA4 Gene

This gene is overexpressed in Skin - Not Sun Exposed (Suprapubic) (x11.0), Skin - Sun Exposed (Lower leg) (x9.1), Esophagus - Mucosa (x7.1), Minor Salivary Gland (x5.9), and Cervix - Ectocervix (x4.6).

Protein differential expression in normal tissues from HIPED for CPA4 Gene

This gene is overexpressed in Bone (36.8) and Amniocyte (25.8).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for CPA4 Gene

NURSA nuclear receptor signaling pathways regulating expression of CPA4 Gene:


SOURCE GeneReport for Unigene cluster for CPA4 Gene:


mRNA Expression by UniProt/SwissProt for CPA4 Gene:

Tissue specificity: Fetal expression in the adrenal gland, brain, heart, intestine, kidney, liver and lung. Except for fetal brain that shows no imprinting, expression was found preferentially from the maternal allele.

Evidence on tissue expression from TISSUES for CPA4 Gene

  • Pancreas(4.2)
  • Kidney(3.7)
  • Nervous system(3.2)
genes like me logo Genes that share expression patterns with CPA4: view

No data available for Protein tissue co-expression partners and Phenotype-based relationships between genes and organs from Gene ORGANizer for CPA4 Gene

Orthologs for CPA4 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for CPA4 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia CPA4 34 33
  • 99.13 (n)
(Canis familiaris)
Mammalia CPA4 34 33
  • 89.15 (n)
(Mus musculus)
Mammalia Cpa4 16 34 33
  • 85.08 (n)
(Rattus norvegicus)
Mammalia Cpa4 33
  • 84.92 (n)
(Bos Taurus)
Mammalia CPA4 34 33
  • 81.27 (n)
(Ornithorhynchus anatinus)
Mammalia -- 34
  • 73 (a)
-- 34
  • 50 (a)
(Monodelphis domestica)
Mammalia CPA4 34
  • 71 (a)
(Gallus gallus)
Aves CPA2 34
  • 60 (a)
(Anolis carolinensis)
Reptilia -- 34
  • 59 (a)
(Danio rerio)
Actinopterygii cpa4 34
  • 57 (a)
cpa2 34
  • 51 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG3108 35 33
  • 50.36 (n)
CG3097 35
  • 35 (a)
CG4017 34 35
  • 33 (a)
CG8563 34 35
  • 32 (a)
CG8560 34 35
  • 32 (a)
CG7025 34 35
  • 31 (a)
CG17633 34 35
  • 30 (a)
CG18585 34 35
  • 30 (a)
CG14820 34 35
  • 30 (a)
CG18415 35
  • 30 (a)
CG18417 34 35
  • 29 (a)
CG8562 34 35
  • 29 (a)
CG32379 34
  • 29 (a)
CG12374 34 35
  • 28 (a)
CG2915 34 35
  • 26 (a)
CG4408 34 35
  • 26 (a)
CG8945 35
  • 26 (a)
CG15769 35
  • 26 (a)
CG8539 34 35
  • 25 (a)
CG8564 34
  • 17 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP000621 33
  • 48.88 (n)
(Caenorhabditis elegans)
Secernentea Y59C2A.1 35
  • 32 (a)
Y18H1A.9 34 35
  • 31 (a)
R11A5.7 35
  • 31 (a)
Y47G6A.19 34 35
  • 29 (a)
F02D8.4 34
  • 28 (a)
W01A8.6 34 35
  • 28 (a)
T06A4.3 34 35
  • 27 (a)
T06A4.1 34
  • 25 (a)
suro-1 34
  • 21 (a)
ZC434.9 34 35
  • 19 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes ECM14 36 34
  • 28 (a)
bread mold
(Neurospora crassa)
Ascomycetes NCU07536 33
  • 49.34 (n)
sea squirt
(Ciona savignyi)
Ascidiacea -- 34
  • 39 (a)
-- 34
  • 39 (a)
CSA.11311 34
  • 38 (a)
CSA.4005 34
  • 20 (a)
Species where no ortholog for CPA4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)

Evolution for CPA4 Gene

Gene Tree for CPA4 (if available)
Gene Tree for CPA4 (if available)
Evolutionary constrained regions (ECRs) for CPA4: view image

Paralogs for CPA4 Gene

Paralogs for CPA4 Gene

(9) SIMAP similar genes for CPA4 Gene using alignment to 7 proteins:

  • A4D1M3_HUMAN
  • B7Z5J4_HUMAN
  • C9J3P4_HUMAN
  • C9J6N7_HUMAN
  • C9J7D6_HUMAN
  • F8WF19_HUMAN
genes like me logo Genes that share paralogs with CPA4: view

Variants for CPA4 Gene

Sequence variations from dbSNP and Humsavar for CPA4 Gene

SNP ID Clin Chr 07 pos Variation AA Info Type
rs1000337607 -- 130,318,646(+) G/C intron_variant
rs1000552890 -- 130,301,545(+) C/T intron_variant
rs1000611902 -- 130,324,048(+) G/A 3_prime_UTR_variant
rs1000674594 -- 130,317,347(+) G/C intron_variant
rs1000873467 -- 130,301,782(+) A/G intron_variant

Structural Variations from Database of Genomic Variants (DGV) for CPA4 Gene

Variant ID Type Subtype PubMed ID
esv3396747 CNV insertion 20981092
nsv1024261 CNV gain 25217958

Variation tolerance for CPA4 Gene

Residual Variation Intolerance Score: 85.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 15.46; 97.16% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for CPA4 Gene

Human Gene Mutation Database (HGMD)
SNPedia medical, phenotypic, and genealogical associations of SNPs for

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CPA4 Gene

Disorders for CPA4 Gene

MalaCards: The human disease database

(2) MalaCards diseases for CPA4 Gene - From: HGMD, DISEASES, and GeneCards

Disorder Aliases PubMed IDs
richter's syndrome
  • richter syndrome
silver-russell syndrome
  • srs
- elite association - COSMIC cancer census association via MalaCards
Search CPA4 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for CPA4

Genetic Association Database
Human Genome Epidemiology Navigator
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
genes like me logo Genes that share disorders with CPA4: view

No data available for UniProtKB/Swiss-Prot and Genatlas for CPA4 Gene

Publications for CPA4 Gene

  1. Characterization of the substrate specificity of human carboxypeptidase A4 and implications for a role in extracellular peptide processing. (PMID: 20385563) Tanco S … Fricker LD (The Journal of biological chemistry 2010) 3 4 22 58
  2. Carboxypeptidase 4 gene variants and early-onset intermediate-to-high risk prostate cancer. (PMID: 19245716) Ross PL … Witte JS (BMC cancer 2009) 3 22 44 58
  3. Structure of human carboxypeptidase A4 with its endogenous protein inhibitor, latexin. (PMID: 15738388) Pallarès I … Gomis-Rüth FX (Proceedings of the National Academy of Sciences of the United States of America 2005) 3 4 22 58
  4. Carboxypeptidase A3 (CPA3): a novel gene highly induced by histone deacetylase inhibitors during differentiation of prostate epithelial cancer cells. (PMID: 10383164) Huang H … Smith DI (Cancer research 1999) 2 3 4 58
  5. Crystal structure of novel metallocarboxypeptidase inhibitor from marine mollusk Nerita versicolor in complex with human carboxypeptidase A4. (PMID: 22294694) Covaleda G … Reverter D (The Journal of biological chemistry 2012) 3 4 58

Products for CPA4 Gene

Sources for CPA4 Gene

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