This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. The encoded preproprotein is proteolytically processed to generate a mature protease that is released by mast cells and may be involved in the degradation of endogenous proteins and the inactivation of venom-associated peptides. Expression of this gene may be elevated in human asthma patients.... See more...

Aliases for CPA3 Gene

Aliases for CPA3 Gene

  • Carboxypeptidase A3 2 3 4 5
  • Mast Cell Carboxypeptidase A 2 3 4
  • Carboxypeptidase A3 (Mast Cell) 2 3
  • Tissue Carboxypeptidase A 2 3
  • MC-CPA 3 4
  • EC 3.4.17.1 4
  • EC 3.4.17 50
  • CPA3 5

External Ids for CPA3 Gene

Previous GeneCards Identifiers for CPA3 Gene

  • GC03P145532
  • GC03P149468
  • GC03P149864
  • GC03P149903
  • GC03P150065
  • GC03P148583
  • GC03P145955

Summaries for CPA3 Gene

Entrez Gene Summary for CPA3 Gene

  • This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. The encoded preproprotein is proteolytically processed to generate a mature protease that is released by mast cells and may be involved in the degradation of endogenous proteins and the inactivation of venom-associated peptides. Expression of this gene may be elevated in human asthma patients. [provided by RefSeq, Nov 2015]

GeneCards Summary for CPA3 Gene

CPA3 (Carboxypeptidase A3) is a Protein Coding gene. Diseases associated with CPA3 include Thiopental Allergy and Factitious Disorder. Among its related pathways are Salivary secretion and Agents Acting on the Renin-Angiotensin System Pathway, Pharmacodynamics. Gene Ontology (GO) annotations related to this gene include peptidase activity and carboxypeptidase activity. An important paralog of this gene is CPB1.

Gene Wiki entry for CPA3 Gene

No data available for CIViC Summary , UniProtKB/Swiss-Prot Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for CPA3 Gene

Genomics for CPA3 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for CPA3 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH03J148865 Promoter/Enhancer 0.6 EPDnew Ensembl 250.7 +0.3 305 0.8 POLR2A CPA3 ENSG00000285557 lnc-HLTF-8 ENSG00000240521 GYG1
GH03J148863 Enhancer 0.3 FANTOM5 258.3 -1.3 -1275 0.1 KLF1 CPA3 HSALNG0029699 ENSG00000285557 lnc-HLTF-8 CPB1 ENSG00000240521
GH03J148866 Enhancer 0.2 Ensembl 250.7 +1.1 1132 0.5 CPA3 ENSG00000285557 lnc-HLTF-8 ENSG00000240521 GYG1
GH03J148790 Promoter/Enhancer 1.3 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas 10.7 -74.5 -74489 4.4 SPI1 GATA2 CTBP1 OSR2 POLR2A CPB1 CPA3 HSALNG0029694 piR-61514-119 AGTR1 ENSG00000285557
GH03J148867 Enhancer 0.3 FANTOM5 Ensembl 16 +2.4 2366 0.3 CPA3 ENSG00000285557 lnc-HLTF-8 ENSG00000240521 GYG1
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around CPA3 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for CPA3

Top Transcription factor binding sites by QIAGEN in the CPA3 gene promoter:
  • FOXJ2
  • FOXJ2 (long isoform)
  • HSF1 (long)
  • HSF1short
  • Ik-1
  • STAT1
  • STAT1alpha
  • STAT1beta
  • TBP
  • USF1

Genomic Locations for CPA3 Gene

Latest Assembly
chr3:148,865,296-148,897,203
(GRCh38/hg38)
Size:
31,908 bases
Orientation:
Plus strand

Previous Assembly
chr3:148,583,083-148,614,990
(GRCh37/hg19 by Entrez Gene)
Size:
31,908 bases
Orientation:
Plus strand

chr3:148,583,043-148,614,983
(GRCh37/hg19 by Ensembl)
Size:
31,941 bases
Orientation:
Plus strand

Genomic View for CPA3 Gene

Genes around CPA3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CPA3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CPA3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CPA3 Gene

Proteins for CPA3 Gene

  • Protein details for CPA3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P15088-CBPA3_HUMAN
    Recommended name:
    Mast cell carboxypeptidase A
    Protein Accession:
    P15088
    Secondary Accessions:
    • Q96E94

    Protein attributes for CPA3 Gene

    Size:
    417 amino acids
    Molecular mass:
    48670 Da
    Cofactor:
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
    Quaternary structure:
    No Data Available

neXtProt entry for CPA3 Gene

Selected DME Specific Peptides for CPA3 Gene

P15088:
  • AFCQWFV
  • GTDLNRNF
  • ESEKETKA
  • GIHAREW

Post-translational modifications for CPA3 Gene

  • Glycosylation at Asn255
  • Modification sites at PhosphoSitePlus
  • Glycosylation from GlyConnect
    • CBPA3_HUMAN (1491)

Other Protein References for CPA3 Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for CPA3 Gene

Gene Families for CPA3 Gene

HGNC:
IUPHAR :
Human Protein Atlas (HPA):
  • Enzymes
  • Plasma proteins
  • Predicted secreted proteins

Protein Domains for CPA3 Gene

InterPro:
Blocks:
  • Zinc carboxypeptidase A metalloprotease (M14)
  • Carboxypeptidase activation peptide

Suggested Antigen Peptide Sequences for CPA3 Gene

GenScript: Design optimal peptide antigens:
  • Carboxypeptidase A3 (CBPA3_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P15088

UniProtKB/Swiss-Prot:

CBPA3_HUMAN :
  • Belongs to the peptidase M14 family.
Family:
  • Belongs to the peptidase M14 family.
genes like me logo Genes that share domains with CPA3: view

Function for CPA3 Gene

Molecular function for CPA3 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=Release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.; EC=3.4.17.1;.
GENATLAS Biochemistry:
carboxypeptidase A3,mast cell,highly induced by histone deacetylase inhibitor during differentiation of prostate epithelial cancer cells

Enzyme Numbers (IUBMB) for CPA3 Gene

Phenotypes From GWAS Catalog for CPA3 Gene

Gene Ontology (GO) - Molecular Function for CPA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004180 carboxypeptidase activity IEA --
GO:0004181 metallocarboxypeptidase activity IBA,TAS 1629626
GO:0008233 peptidase activity IEA --
GO:0008237 metallopeptidase activity IEA --
GO:0008270 zinc ion binding IEA --
genes like me logo Genes that share ontologies with CPA3: view
genes like me logo Genes that share phenotypes with CPA3: view

Animal Models for CPA3 Gene

MGI Knock Outs for CPA3:
  • Cpa3 Cpa3<tm1Hrr>
  • Cpa3 Cpa3<tm1.1(KOMP)Vlcg>

Animal Models for research

  • Taconic Biosciences Mouse Models for CPA3

miRNA for CPA3 Gene

miRTarBase miRNAs that target CPA3

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CPA3

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for CPA3 Gene

Localization for CPA3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CPA3 Gene

Cytoplasmic vesicle, secretory vesicle. Note=Secretory granules.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CPA3 gene
Compartment Confidence
extracellular 4
plasma membrane 2
lysosome 2
cytoskeleton 1
mitochondrion 1
nucleus 1
endoplasmic reticulum 1
cytosol 1
golgi apparatus 1
peroxisome 0

Subcellular locations from the

Human Protein Atlas (HPA)
  • Golgi apparatus (2)
  • Nucleoplasm (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for CPA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005576 extracellular region HDA,TAS --
GO:0005615 extracellular space IBA 21873635
GO:0030133 transport vesicle IEA --
GO:0030141 secretory granule NAS 2594780
GO:0031410 cytoplasmic vesicle IEA --
genes like me logo Genes that share ontologies with CPA3: view

Pathways & Interactions for CPA3 Gene

genes like me logo Genes that share pathways with CPA3: view

Gene Ontology (GO) - Biological Process for CPA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002002 regulation of angiotensin levels in blood IEA --
GO:0002003 angiotensin maturation TAS --
GO:0006508 proteolysis IBA,TAS 2708524
genes like me logo Genes that share ontologies with CPA3: view

No data available for SIGNOR curated interactions for CPA3 Gene

Drugs & Compounds for CPA3 Gene

(2) Drugs for CPA3 Gene - From: HMDB and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Zinc Approved, Investigational Pharma 1706
genes like me logo Genes that share compounds with CPA3: view

Transcripts for CPA3 Gene

mRNA/cDNA for CPA3 Gene

1 REFSEQ mRNAs :
8 NCBI additional mRNA sequence :
2 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CPA3

Alternative Splicing Database (ASD) splice patterns (SP) for CPA3 Gene

No ASD Table

Relevant External Links for CPA3 Gene

GeneLoc Exon Structure for
CPA3

Expression for CPA3 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for CPA3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for CPA3 Gene

This gene is overexpressed in Lung (x4.9).

Protein differential expression in normal tissues from HIPED for CPA3 Gene

This gene is overexpressed in Rectum (12.6), Urinary Bladder (11.3), Gallbladder (9.9), and Adipocyte (8.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for CPA3 Gene



Protein tissue co-expression partners for CPA3 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for CPA3

SOURCE GeneReport for Unigene cluster for CPA3 Gene:

Hs.646

Evidence on tissue expression from TISSUES for CPA3 Gene

  • Lung(4.5)
  • Bone marrow(4.5)
  • Intestine(2.3)
  • Stomach(2.3)
genes like me logo Genes that share expression patterns with CPA3: view

Primer products for research

No data available for mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for CPA3 Gene

Orthologs for CPA3 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for CPA3 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia CPA3 29 30
  • 99.2 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia CPA3 29 30
  • 90.73 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia CPA3 29 30
  • 89.21 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Cpa3 29
  • 83.53 (n)
Mouse
(Mus musculus)
Mammalia Cpa3 29 16 30
  • 83.37 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia CPA3 30
  • 67 (a)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia -- 30
  • 55 (a)
OneToMany
-- 30
  • 51 (a)
OneToMany
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia cpb1 29
  • 61.31 (n)
Zebrafish
(Danio rerio)
Actinopterygii cpb1 30
  • 47 (a)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP013181 29
  • 44.27 (n)
Fruit Fly
(Drosophila melanogaster)
Insecta CG18585 30 31
  • 31 (a)
ManyToMany
CG4017 30 31
  • 31 (a)
ManyToMany
CG3097 31
  • 31 (a)
CG3108 31
  • 31 (a)
CG14820 30 31
  • 30 (a)
ManyToMany
CG8562 30 31
  • 29 (a)
ManyToMany
CG32379 30
  • 29 (a)
ManyToMany
CG7025 30 31
  • 29 (a)
ManyToMany
CG18415 31
  • 29 (a)
CG2915 30 31
  • 28 (a)
ManyToMany
CG17633 30 31
  • 28 (a)
ManyToMany
CG18417 30 31
  • 28 (a)
ManyToMany
CG15769 31
  • 28 (a)
CG8945 31
  • 27 (a)
CG12374 30 31
  • 26 (a)
ManyToMany
CG8560 30
  • 26 (a)
ManyToMany
CG4408 30 31
  • 25 (a)
ManyToMany
CG8563 30
  • 24 (a)
ManyToMany
CG8539 30
  • 21 (a)
ManyToMany
CG8564 30 31
  • 17 (a)
ManyToMany
Worm
(Caenorhabditis elegans)
Secernentea Y59C2A.1 31
  • 31 (a)
R11A5.7 31
  • 30 (a)
Y18H1A.9 30 31
  • 29 (a)
ManyToMany
T06A4.3 30 31
  • 27 (a)
ManyToMany
F02D8.4 30
  • 26 (a)
ManyToMany
Y47G6A.19 30
  • 25 (a)
ManyToMany
T06A4.1 30
  • 24 (a)
ManyToMany
W01A8.6 30
  • 24 (a)
ManyToMany
suro-1 30
  • 19 (a)
ManyToMany
ZC434.9 30
  • 17 (a)
ManyToMany
K. Lactis Yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0F27929g 29
  • 46.72 (n)
A. gosspyii yeast
(Eremothecium gossypii)
Saccharomycetes AGOS_AFR045W 29
  • 43.96 (n)
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes ECM14 29 30
  • 43.56 (n)
OneToMany
Fission Yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes SPBC337.07c 29
  • 46.21 (n)
Bread mold
(Neurospora crassa)
Ascomycetes NCU01464 29
  • 44.87 (n)
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 30
  • 34 (a)
ManyToMany
-- 30
  • 34 (a)
ManyToMany
CSA.11311 30
  • 34 (a)
ManyToMany
CSA.4005 30
  • 17 (a)
ManyToMany
Species where no ortholog for CPA3 was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Platypus (Ornithorhynchus anatinus)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for CPA3 Gene

ENSEMBL:
Gene Tree for CPA3 (if available)
TreeFam:
Gene Tree for CPA3 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for CPA3: view image
Alliance of Genome Resources:
Additional Orthologs for CPA3

Paralogs for CPA3 Gene

Paralogs for CPA3 Gene

(9) SIMAP similar genes for CPA3 Gene using alignment to 1 proteins:

  • CBPA3_HUMAN
genes like me logo Genes that share paralogs with CPA3: view

Variants for CPA3 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for CPA3 Gene

SNP ID Clinical significance and condition Chr 03 pos Variation AA Info Type
rs140470073 Benign: not provided 148,883,682(+) C/T
NM_001870.4(CPA3):c.848C>T (p.Thr283Met)
MISSENSE
rs143184657 Benign: not provided 148,883,652(+) G/C
NM_001870.4(CPA3):c.818G>C (p.Arg273Pro)
MISSENSE
rs367753806 Likely Benign: not provided 148,878,721(+) T/G
NM_001870.4(CPA3):c.447T>G (p.Val149=)
SYNONYMOUS
rs78356818 Benign: not provided 148,881,557(+) C/G
NM_001870.4(CPA3):c.612C>G (p.Thr204=)
SYNONYMOUS
rs12489516 -- p.Thr171Met

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for CPA3 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for CPA3 Gene

Variant ID Type Subtype PubMed ID
esv3349668 CNV insertion 20981092
esv3598135 CNV loss 21293372
nsv829751 CNV loss 17160897

Variation tolerance for CPA3 Gene

Residual Variation Intolerance Score: 95.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.26; 62.52% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for CPA3 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
CPA3
Leiden Open Variation Database (LOVD)
CPA3

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CPA3 Gene

Disorders for CPA3 Gene

MalaCards: The human disease database

(4) MalaCards diseases for CPA3 Gene - From: COP and GCD

Disorder Aliases PubMed IDs
thiopental allergy
  • penthiobarbital allergy
factitious disorder
  • munchausen syndrome
asthma
  • asthma, susceptibility to
esophagitis, eosinophilic, 1
  • eoe1
- elite association - COSMIC cancer census association via MalaCards
Search CPA3 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for CPA3

genes like me logo Genes that share disorders with CPA3: view

No data available for UniProtKB/Swiss-Prot and Genatlas for CPA3 Gene

Publications for CPA3 Gene

  1. Human skin mast cell carboxypeptidase: functional characterization, cDNA cloning, and genealogy. (PMID: 1629626) Natsuaki M … Goldstein SM (The Journal of investigative dermatology 1992) 3 4 22
  2. Cloning and characterization of the novel gene for mast cell carboxypeptidase A. (PMID: 1729276) Reynolds DS … Austen KF (The Journal of clinical investigation 1992) 3 4 22
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4
  4. Cloning of cDNAs that encode human mast cell carboxypeptidase A, and comparison of the protein with mouse mast cell carboxypeptidase A and rat pancreatic carboxypeptidases. (PMID: 2594780) Reynolds DS … Serafin WE (Proceedings of the National Academy of Sciences of the United States of America 1989) 3 4
  5. Human mast cell carboxypeptidase. Purification and characterization. (PMID: 2708524) Goldstein SM … Wintroub BU (The Journal of clinical investigation 1989) 3 4

Products for CPA3 Gene

Sources for CPA3 Gene