Aliases for CNOT8 Gene

Aliases for CNOT8 Gene

  • CCR4-NOT Transcription Complex Subunit 8 2 3 4 5
  • CALIF 2 3 4
  • PGK Promoter Directed Over Production 2 3
  • CCR4-Associated Factor 8 3 4
  • CAF1-Like Protein 3 4
  • CALIFp 3 4
  • Caf1b 3 4
  • HCAF1 2 3
  • CAF1 2 3
  • POP2 3 4
  • CAF2 3 4
  • CCR4-NOT Transcription Complex, Subunit 8 2
  • EC 3.1.13.4 4
  • CNOT8 5

External Ids for CNOT8 Gene

Previous HGNC Symbols for CNOT8 Gene

  • POP2

Previous GeneCards Identifiers for CNOT8 Gene

  • GC05P154331
  • GC05P154833
  • GC05P154221
  • GC05P154266
  • GC05P154218
  • GC05P149380

Summaries for CNOT8 Gene

GeneCards Summary for CNOT8 Gene

CNOT8 (CCR4-NOT Transcription Complex Subunit 8) is a Protein Coding gene. Diseases associated with CNOT8 include Amyotrophic Lateral Sclerosis 1. Among its related pathways are Deadenylation-dependent mRNA decay and Gene Expression. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and RNA binding. An important paralog of this gene is CNOT7.

UniProtKB/Swiss-Prot Summary for CNOT8 Gene

  • Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT7. Catalytic component of the CCR4-NOT complex which is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.

Gene Wiki entry for CNOT8 Gene

Additional gene information for CNOT8 Gene

No data available for Entrez Gene Summary , CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for CNOT8 Gene

Genomics for CNOT8 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for CNOT8 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH05J154856 Promoter/Enhancer 2.4 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 605.4 +2.0 2033 6.3 HNRNPL CREB1 GATAD2A TEAD4 PRDM10 ZNF629 TFE3 RFX1 LEF1 IKZF1 CNOT8 FAXDC2 SAP30L-AS1 lnc-CNOT8-2 HE856236 RF00017-5030 GEMIN5
GH05J154834 Promoter/Enhancer 1.6 Ensembl ENCODE dbSUPER 34.5 -22.5 -22471 1.7 GATAD2A PRDM10 LEF1 IKZF1 ZNF692 JUND ZNF300 FOXA1 PRDM1 DDX20 SAP30L-AS1 CNOT8 LARP1 MFAP3 GEMIN5 FAM114A2 FAXDC2 MIR378H piR-32286-002 lnc-GEMIN5-1
GH05J154752 Promoter/Enhancer 2.1 Ensembl ENCODE CraniofacialAtlas dbSUPER 25.1 -100.1 -100070 9.1 SP1 HNRNPL CREB1 GATAD2A PRDM10 ZNF629 TFE3 LEF1 IKZF1 NFKBIZ SAP30L-AS1 CNOT8 GEMIN5 LARP1 MIR1303 FAM114A2 LOC112267936 lnc-FAXDC2-4 RF00017-5028
GH05J154769 Enhancer 0.8 ENCODE dbSUPER 32.4 -86.9 -86867 2.9 PRDM10 IKZF1 POLR2A ZNF600 NONO OSR2 ZBTB17 CTCF RCOR1 RAD21 CNOT8 LARP1 FAXDC2 RF00017-5028 piR-33330
GH05J154812 Enhancer 1 Ensembl ENCODE dbSUPER 24.3 -44.5 -44459 2.6 CTCF PRDM1 ZNF143 REST RAD21 ZNF740 TRIM22 HDAC1 SMARCA5 ZNF444 CNOT8 piR-33330 RF00017-5028 LARP1
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around CNOT8 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for CNOT8

Top Transcription factor binding sites by QIAGEN in the CNOT8 gene promoter:
  • ARP-1
  • FOXD1
  • GATA-1
  • HNF-3beta
  • HNF-4alpha1
  • HNF-4alpha2
  • Lhx3a
  • LHX3b
  • N-Myc
  • USF-1

Genomic Locations for CNOT8 Gene

Genomic Locations for CNOT8 Gene
chr5:154,857,553-154,876,793
(GRCh38/hg38)
Size:
19,241 bases
Orientation:
Plus strand
chr5:154,237,113-154,256,353
(GRCh37/hg19)
Size:
19,241 bases
Orientation:
Plus strand

Genomic View for CNOT8 Gene

Genes around CNOT8 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CNOT8 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CNOT8 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CNOT8 Gene

Proteins for CNOT8 Gene

  • Protein details for CNOT8 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9UFF9-CNOT8_HUMAN
    Recommended name:
    CCR4-NOT transcription complex subunit 8
    Protein Accession:
    Q9UFF9
    Secondary Accessions:
    • B0AZS3
    • B2RAR8
    • B7Z8R1
    • D3DQI8
    • O95709
    • Q7Z521
    • Q9H6Y1

    Protein attributes for CNOT8 Gene

    Size:
    292 amino acids
    Molecular mass:
    33540 Da
    Quaternary structure:
    • Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits; the complex contains two deadenylase subunits, CNOT6 or CNOT6L, and CNOT7 or CNOT8. In the complex interacts directly with CNOT1. Interacts with BTG1, BTG2 and TOB1.

    Alternative splice isoforms for CNOT8 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for CNOT8 Gene

Post-translational modifications for CNOT8 Gene

  • Ubiquitination at Lys206, Lys242, Lys254, and Lys269
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for CNOT8 Gene

Domains & Families for CNOT8 Gene

Gene Families for CNOT8 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for CNOT8 Gene

InterPro:
Blocks:
  • CAF1 family ribonuclease
ProtoNet:

Suggested Antigen Peptide Sequences for CNOT8 Gene

GenScript: Design optimal peptide antigens:
  • cDNA, FLJ79513, highly similar to CCR4-NOT transcription complex subunit 8 (B0AZS3_HUMAN)
  • cDNA FLJ52273, highly similar to CCR4-NOT transcription complex subunit 8 (B7Z5Z0_HUMAN)
  • cDNA FLJ52044, moderately similar to CCR4-NOT transcription complex subunit 8 (B7Z8R1_HUMAN)
  • cDNA, FLJ78954, highly similar to CCR4-NOT transcription complex subunit 8 (B7Z9U0_HUMAN)
  • CCR4-associated factor 8 (CNOT8_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9UFF9

UniProtKB/Swiss-Prot:

CNOT8_HUMAN :
  • Belongs to the CAF1 family.
Family:
  • Belongs to the CAF1 family.
genes like me logo Genes that share domains with CNOT8: view

Function for CNOT8 Gene

Molecular function for CNOT8 Gene

UniProtKB/Swiss-Prot Function:
Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate. Its function seems to be partially redundant with that of CNOT7. Catalytic component of the CCR4-NOT complex which is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation. Additional complex functions may be a consequence of its influence on mRNA expression. Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=Exonucleolytic cleavage of poly(A) to 5'-AMP.; EC=3.1.13.4; Evidence={ECO:0000269|PubMed:15769875};.

Enzyme Numbers (IUBMB) for CNOT8 Gene

Phenotypes From GWAS Catalog for CNOT8 Gene

Gene Ontology (GO) - Molecular Function for CNOT8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000175 3'-5'-exoribonuclease activity IDA 15769875
GO:0003676 nucleic acid binding IEA --
GO:0003700 DNA-binding transcription factor activity NAS 10036195
GO:0003723 RNA binding IEA --
GO:0004518 nuclease activity IEA --
genes like me logo Genes that share ontologies with CNOT8: view
genes like me logo Genes that share phenotypes with CNOT8: view

Animal Model Products

CRISPR Products

miRNA for CNOT8 Gene

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CNOT8

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for CNOT8 Gene

Localization for CNOT8 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CNOT8 Gene

Cytoplasm. Nucleus.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CNOT8 gene
Compartment Confidence
nucleus 4
cytosol 4
cytoskeleton 3
extracellular 2
plasma membrane 1
mitochondrion 1
endoplasmic reticulum 1

Gene Ontology (GO) - Cellular Components for CNOT8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000932 P-body IBA 21873635
GO:0005622 intracellular IDA --
GO:0005634 nucleus IDA,NAS 10036195
GO:0005737 cytoplasm IEA --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with CNOT8: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for CNOT8 Gene

Pathways & Interactions for CNOT8 Gene

genes like me logo Genes that share pathways with CNOT8: view

Gene Ontology (GO) - Biological Process for CNOT8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening TAS --
GO:0006351 transcription, DNA-templated IEA --
GO:0006355 regulation of transcription, DNA-templated NAS 10036195
GO:0006417 regulation of translation IEA --
GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest TAS --
genes like me logo Genes that share ontologies with CNOT8: view

No data available for SIGNOR curated interactions for CNOT8 Gene

Drugs & Compounds for CNOT8 Gene

No Compound Related Data Available

Transcripts for CNOT8 Gene

mRNA/cDNA for CNOT8 Gene

9 REFSEQ mRNAs :
20 NCBI additional mRNA sequence :
25 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CNOT8

Alternative Splicing Database (ASD) splice patterns (SP) for CNOT8 Gene

ExUns: 1a · 1b · 1c · 1d · 1e · 1f ^ 2a · 2b · 2c · 2d · 2e · 2f · 2g · 2h ^ 3a · 3b · 3c ^ 4a · 4b · 4c ^ 5a · 5b · 5c ^ 6a · 6b ^ 7a ·
SP1: - - - - - - - - - -
SP2: - - - - - - - -
SP3: - - - - - - - - - - - - -
SP4: - - - - - - - - - - -
SP5: - - - - - - - - - - - -
SP6: - - - - - - - - - -
SP7: - - - - - - - - - - - - -
SP8: - - - - - - - - - - - -
SP9: - - - - - - - - -
SP10: - - - - - - -
SP11: -
SP12: - - - - - - - - - - - -
SP13: - - - - - - - - - - -
SP14: - - -
SP15: - - - - - - - - - - - - - -
SP16: - - - - - - - - - -
SP17: - - - - - - - - - - - -

ExUns: 7b ^ 8a · 8b ^ 9 ^ 10 ^ 11a · 11b · 11c · 11d · 11e
SP1: -
SP2: -
SP3: -
SP4: -
SP5: -
SP6: - - -
SP7: - - -
SP8:
SP9: -
SP10: - - -
SP11:
SP12: -
SP13:
SP14:
SP15:
SP16:
SP17: -

Relevant External Links for CNOT8 Gene

GeneLoc Exon Structure for
CNOT8

Expression for CNOT8 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for CNOT8 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for CNOT8 Gene

This gene is overexpressed in Bone (40.5) and Breast (23.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for CNOT8 Gene



Protein tissue co-expression partners for CNOT8 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for CNOT8

SOURCE GeneReport for Unigene cluster for CNOT8 Gene:

Hs.26703

Evidence on tissue expression from TISSUES for CNOT8 Gene

  • Nervous system(4.9)
  • Intestine(4.5)
  • Kidney(4.4)
  • Liver(4.4)
  • Skin(2.3)
  • Spleen(2.2)
  • Blood(2.2)
  • Heart(2.2)
  • Lymph node(2.2)
  • Bone marrow(2.1)
  • Thyroid gland(2.1)
  • Lung(2.1)
genes like me logo Genes that share expression patterns with CNOT8: view

No data available for mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for CNOT8 Gene

Orthologs for CNOT8 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for CNOT8 Gene

Organism Taxonomy Gene Similarity Type Details
Platypus
(Ornithorhynchus anatinus)
Mammalia CNOT8 31
  • 100 (a)
OneToOne
Chimpanzee
(Pan troglodytes)
Mammalia CNOT8 30 31
  • 99.89 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia CNOT8 30 31
  • 96 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia CNOT8 30 31
  • 94.98 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia CNOT8 31
  • 93 (a)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Cnot8 30
  • 89.38 (n)
Mouse
(Mus musculus)
Mammalia Cnot8 30 17 31
  • 88.47 (n)
OneToOne
Chicken
(Gallus gallus)
Aves CNOT8 30 31
  • 84.08 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia CNOT8 31
  • 97 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia cnot8 30
  • 78.78 (n)
Str.6603 30
African clawed frog
(Xenopus laevis)
Amphibia cnot8-prov 30
Zebrafish
(Danio rerio)
Actinopterygii cnot8 30 31
  • 76.36 (n)
OneToOne
wufe49a05 30
Rainbow Trout
(Oncorhynchus mykiss)
Actinopterygii Omy.467 30
Fruit Fly
(Drosophila melanogaster)
Insecta CG5684 32
  • 72 (a)
Pop2 31
  • 50 (a)
OneToMany
Worm
(Caenorhabditis elegans)
Secernentea ccf-1 31
  • 42 (a)
OneToMany
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes POP2 31 33
  • 24 (a)
OneToMany
Alicante grape
(Vitis vinifera)
eudicotyledons Vvi.4584 30
Rice
(Oryza sativa)
Liliopsida Os09g0416800 30
  • 53.77 (n)
Corn
(Zea mays)
Liliopsida Zm.15431 30
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 66 (a)
OneToMany
Species where no ortholog for CNOT8 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for CNOT8 Gene

ENSEMBL:
Gene Tree for CNOT8 (if available)
TreeFam:
Gene Tree for CNOT8 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for CNOT8: view image

Paralogs for CNOT8 Gene

Paralogs for CNOT8 Gene

(1) SIMAP similar genes for CNOT8 Gene using alignment to 14 proteins:

  • CNOT8_HUMAN
  • B0AZS3_HUMAN
  • B7Z8R1_HUMAN
  • B7Z9U0_HUMAN
  • E5RFJ4_HUMAN
  • E5RGE4_HUMAN
  • E5RHD6_HUMAN
  • E5RHG0_HUMAN
  • E5RHL9_HUMAN
  • E5RHP3_HUMAN
  • E5RIL2_HUMAN
  • E5RIU0_HUMAN
  • E5RJC5_HUMAN
  • E5RK92_HUMAN

Pseudogenes.org Pseudogenes for CNOT8 Gene

genes like me logo Genes that share paralogs with CNOT8: view

Variants for CNOT8 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for CNOT8 Gene

SNP ID Clinical significance and condition Chr 05 pos Variation AA Info Type
rs1139980 - p.Leu32Pro

Additional dbSNP identifiers (rs#s) for CNOT8 Gene

Structural Variations from Database of Genomic Variants (DGV) for CNOT8 Gene

Variant ID Type Subtype PubMed ID
nsv1073926 CNV deletion 25765185
nsv830521 CNV gain 17160897

Variation tolerance for CNOT8 Gene

Residual Variation Intolerance Score: 36.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.43; 9.40% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for CNOT8 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
CNOT8

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CNOT8 Gene

Disorders for CNOT8 Gene

MalaCards: The human disease database

(1) MalaCards diseases for CNOT8 Gene - From: DISEASES

Disorder Aliases PubMed IDs
amyotrophic lateral sclerosis 1
  • als1
- elite association - COSMIC cancer census association via MalaCards
Search CNOT8 in MalaCards View complete list of genes associated with diseases
genes like me logo Genes that share disorders with CNOT8: view

No data available for UniProtKB/Swiss-Prot and Genatlas for CNOT8 Gene

Publications for CNOT8 Gene

  1. Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits. (PMID: 10637334) Albert TK … Timmers HT (Nucleic acids research 2000) 2 3 4 23
  2. CCR4-NOT deadenylates mRNA associated with RNA-induced silencing complexes in human cells. (PMID: 20065043) Piao X … Belasco JG (Molecular and cellular biology 2010) 3 4 23
  3. The Ccr4-NOT deadenylase subunits CNOT7 and CNOT8 have overlapping roles and modulate cell proliferation. (PMID: 19605561) Aslam A … Winkler GS (Molecular biology of the cell 2009) 3 4 23
  4. Conservation of the deadenylase activity of proteins of the Caf1 family in human. (PMID: 15769875) Bianchin C … Corbo L (RNA (New York, N.Y.) 2005) 3 4 23
  5. Relationships of the antiproliferative proteins BTG1 and BTG2 with CAF1, the human homolog of a component of the yeast CCR4 transcriptional complex: involvement in estrogen receptor alpha signaling pathway. (PMID: 11136725) Prévôt D … Corbo L (The Journal of biological chemistry 2001) 3 4 23

Products for CNOT8 Gene

  • Signalway Proteins for CNOT8

Sources for CNOT8 Gene