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Aliases for CLIC4 Gene

Aliases for CLIC4 Gene

  • Chloride Intracellular Channel 4 2 3 5
  • Intracellular Chloride Ion Channel Protein P64H1 3 4
  • Chloride Intracellular Channel Protein 4 3 4
  • Epididymis Secretory Sperm Binding Protein 3
  • Chloride Intracellular Channel 4 Like 3
  • CLIC4L 3
  • MTCLIC 3
  • P64H1 3
  • HuH1 3
  • H1 3

External Ids for CLIC4 Gene

Previous GeneCards Identifiers for CLIC4 Gene

  • GC01P024596
  • GC01P024141
  • GC01P024423
  • GC01P024817
  • GC01P024944
  • GC01P025071
  • GC01P023328

Summaries for CLIC4 Gene

Entrez Gene Summary for CLIC4 Gene

  • Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq, Jul 2008]

GeneCards Summary for CLIC4 Gene

CLIC4 (Chloride Intracellular Channel 4) is a Protein Coding gene. Among its related pathways are Activation of cAMP-Dependent PKA and Glucose / Energy Metabolism. Gene Ontology (GO) annotations related to this gene include chloride channel activity and voltage-gated chloride channel activity. An important paralog of this gene is CLIC5.

UniProtKB/Swiss-Prot for CLIC4 Gene

  • Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).

Gene Wiki entry for CLIC4 Gene

Additional gene information for CLIC4 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CLIC4 Gene

Genomics for CLIC4 Gene

GeneHancer (GH) Regulatory Elements for CLIC4 Gene

Promoters and enhancers for CLIC4 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH01J024740 Promoter/Enhancer 2.3 EPDnew Ensembl ENCODE dbSUPER 610.4 +0.2 181 9.7 SP1 ZFX ELF3 MNT CBFA2T2 NKRF POLR2A MLLT1 ZNF121 CEBPG CLIC4 GC01M024778 RNU6-1208P
GH01J024712 Enhancer 1 Ensembl ENCODE dbSUPER 22.1 -31.0 -30986 3.4 CBFA2T2 MZF1 EGR1 ZNF316 CEBPB JUND FOS ZFHX2 FOSL2 EGR2 CLIC4 RNU6-1208P SRRM1 ENSG00000284699
GH01J024692 Enhancer 1.6 FANTOM5 Ensembl ENCODE dbSUPER 9 -50.4 -50442 4.7 CC2D1A ZNF121 ZNF687 ZSCAN21 NCOA3 TRIM24 SP7 MLLT1 ZNF639 SMARCA4 CLIC4 GC01P024703 SRRM1
GH01J024765 Enhancer 1.1 ENCODE dbSUPER 12.2 +20.5 20463 1.2 CC2D1A ZKSCAN8 MLLT1 CSDE1 ZNF384 BACH1 MAFK TRIM24 ZNF639 SMARCA4 SRSF10 SRRM1 CLIC4 PNRC2 NCMAP GC01M024778 RNU6-1208P
GH01J024682 Enhancer 1.1 Ensembl ENCODE dbSUPER 12.5 -61.7 -61717 2.1 ZNF121 SP7 ZNF384 SMARCA4 POLR2A DPF2 ZNF600 JUN SP1 JUND CLIC4 RNU6-1208P NCMAP GC01P024703 SRRM1
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around CLIC4 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the CLIC4 gene promoter:
  • C/EBPbeta
  • Evi-1
  • FOXJ2
  • FOXJ2 (long isoform)
  • FOXO3a
  • FOXO3b
  • Pax-5
  • S8
  • Zic1

Genomic Locations for CLIC4 Gene

Genomic Locations for CLIC4 Gene
chr1:24,745,269-24,844,324
(GRCh38/hg38)
Size:
99,056 bases
Orientation:
Plus strand
chr1:25,071,760-25,170,815
(GRCh37/hg19)
Size:
99,056 bases
Orientation:
Plus strand

Genomic View for CLIC4 Gene

Genes around CLIC4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CLIC4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CLIC4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CLIC4 Gene

Proteins for CLIC4 Gene

  • Protein details for CLIC4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9Y696-CLIC4_HUMAN
    Recommended name:
    Chloride intracellular channel protein 4
    Protein Accession:
    Q9Y696
    Secondary Accessions:
    • Q9UFW9
    • Q9UQJ6

    Protein attributes for CLIC4 Gene

    Size:
    253 amino acids
    Molecular mass:
    28772 Da
    Quaternary structure:
    • Component of a multimeric complex consisting of several cytoskeletal proteins, including actin, ezrin, alpha-actinin, gelsolin, IQGAP1 and CLIC5A. Binds directly to brain dynamin I in a complex containing actin, tubulin and 14-3-3 isoforms. Monomer. Interacts with HRH3. Interacts with AKAP9.

    Three dimensional structures from OCA and Proteopedia for CLIC4 Gene

neXtProt entry for CLIC4 Gene

Post-translational modifications for CLIC4 Gene

  • Ubiquitination at isoforms=199, isoforms=212, posLast=6262, and isoforms=146
  • Modification sites at PhosphoSitePlus

Other Protein References for CLIC4 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for CLIC4 Gene

Domains & Families for CLIC4 Gene

Gene Families for CLIC4 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins
  • Transporters

Suggested Antigen Peptide Sequences for CLIC4 Gene

GenScript: Design optimal peptide antigens:
  • Intracellular chloride ion channel protein p64H1 (CLIC4_HUMAN)
  • cDNA, FLJ96000, Homo sapiens chloride intracellular channel 4 (CLIC4), mRNA (Q6FIC5_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9Y696

UniProtKB/Swiss-Prot:

CLIC4_HUMAN :
  • Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
  • Belongs to the chloride channel CLIC family.
Domain:
  • Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.
Family:
  • Belongs to the chloride channel CLIC family.
genes like me logo Genes that share domains with CLIC4: view

Function for CLIC4 Gene

Molecular function for CLIC4 Gene

UniProtKB/Swiss-Prot Function:
Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).
UniProtKB/Swiss-Prot Induction:
Up-regulated by calcium ions in differentiating keratinocytes. Up-regulated in myofibroblasts.

Phenotypes From GWAS Catalog for CLIC4 Gene

Gene Ontology (GO) - Molecular Function for CLIC4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005244 voltage-gated ion channel activity IEA --
GO:0005254 chloride channel activity IBA 21873635
GO:0005515 protein binding IPI 10793131
genes like me logo Genes that share ontologies with CLIC4: view
genes like me logo Genes that share phenotypes with CLIC4: view

Animal Models for CLIC4 Gene

MGI Knock Outs for CLIC4:

Animal Model Products

  • Taconic Biosciences Mouse Models for CLIC4

CRISPR Products

miRNA for CLIC4 Gene

miRTarBase miRNAs that target CLIC4

Clone Products

  • Applied Biological Materials (abm): Clones for CLIC4 - Now 50% OFF >
  • * CLIC4 as ready-to-use vector or virus: ORF | Lenti- | Retro- | Adeno- | AAV- | Protein Vector - Browse All
  • * CLIC4 tags and reporters available: His, HA, Myc, Flag, GFP, RFP, Luciferase - Browse All

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for CLIC4 Gene

Localization for CLIC4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CLIC4 Gene

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic vesicle membrane; Single-pass membrane protein. Nucleus matrix. Cell membrane; Single-pass membrane protein. Mitochondrion. Cell junction. Note=Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CLIC4 gene
Compartment Confidence
plasma membrane 5
cytoskeleton 5
mitochondrion 5
nucleus 5
cytosol 5
extracellular 1
endoplasmic reticulum 1
endosome 1
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for CLIC4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005622 intracellular IDA --
GO:0005634 nucleus IBA 21873635
GO:0005737 cytoplasm IDA,IEA 10793131
GO:0005739 mitochondrion IBA,IDA 14569596
GO:0005813 centrosome IDA 14569596
genes like me logo Genes that share ontologies with CLIC4: view

Pathways & Interactions for CLIC4 Gene

genes like me logo Genes that share pathways with CLIC4: view

Pathways by source for CLIC4 Gene

1 Cell Signaling Technology pathway for CLIC4 Gene

Gene Ontology (GO) - Biological Process for CLIC4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001525 angiogenesis IBA 21873635
GO:0001886 endothelial cell morphogenesis IEA --
GO:0006811 ion transport IEA --
GO:0006821 chloride transport NAS 10793131
GO:0007035 vacuolar acidification IEA --
genes like me logo Genes that share ontologies with CLIC4: view

No data available for SIGNOR curated interactions for CLIC4 Gene

Drugs & Compounds for CLIC4 Gene

(1) Drugs for CLIC4 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Chloride ion Experimental Pharma 0

(1) Additional Compounds for CLIC4 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with CLIC4: view

Transcripts for CLIC4 Gene

mRNA/cDNA for CLIC4 Gene

Unigene Clusters for CLIC4 Gene

Chloride intracellular channel 4:
Representative Sequences:

CRISPR Products

Clone Products

  • Applied Biological Materials (abm): Clones for CLIC4 - Now 50% OFF >
  • * CLIC4 as ready-to-use vector or virus: ORF | Lenti- | Retro- | Adeno- | AAV- | Protein Vector - Browse All
  • * CLIC4 tags and reporters available: His, HA, Myc, Flag, GFP, RFP, Luciferase - Browse All

Alternative Splicing Database (ASD) splice patterns (SP) for CLIC4 Gene

ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6a · 6b
SP1:
SP2: - -

Relevant External Links for CLIC4 Gene

GeneLoc Exon Structure for
CLIC4
ECgene alternative splicing isoforms for
CLIC4

Expression for CLIC4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for CLIC4 Gene

mRNA differential expression in normal tissues according to GTEx for CLIC4 Gene

This gene is overexpressed in Artery - Tibial (x5.1) and Artery - Aorta (x4.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for CLIC4 Gene



Protein tissue co-expression partners for CLIC4 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of CLIC4 Gene:

CLIC4

SOURCE GeneReport for Unigene cluster for CLIC4 Gene:

Hs.440544

mRNA Expression by UniProt/SwissProt for CLIC4 Gene:

Q9Y696-CLIC4_HUMAN
Tissue specificity: Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle.

Evidence on tissue expression from TISSUES for CLIC4 Gene

  • Kidney(4.8)
  • Nervous system(4.8)
  • Liver(4.5)
  • Blood(4.2)
  • Skin(3.2)
  • Heart(3.1)
  • Lung(2.8)
  • Muscle(2)
  • Spleen(2)
genes like me logo Genes that share expression patterns with CLIC4: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , Protein differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for CLIC4 Gene

Orthologs for CLIC4 Gene

This gene was present in the common ancestor of animals.

Orthologs for CLIC4 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia CLIC4 35 34
  • 99.87 (n)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia CLIC4 35
  • 98 (a)
OneToOne
dog
(Canis familiaris)
Mammalia CLIC4 35 34
  • 95.52 (n)
OneToOne
cow
(Bos Taurus)
Mammalia CLIC4 35 34
  • 94.73 (n)
OneToOne
rat
(Rattus norvegicus)
Mammalia Clic4 34
  • 88.93 (n)
mouse
(Mus musculus)
Mammalia Clic4 17 35 34
  • 88.41 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia CLIC4 35
  • 83 (a)
OneToOne
chicken
(Gallus gallus)
Aves CLIC4 35 34
  • 78.4 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia CLIC4 35
  • 89 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia clic5 34
  • 76.46 (n)
Str.20225 34
African clawed frog
(Xenopus laevis)
Amphibia Xl.3485 34
zebrafish
(Danio rerio)
Actinopterygii clic4 35 34
  • 75 (n)
OneToOne
wufc47b04 34
fruit fly
(Drosophila melanogaster)
Insecta Clic 35
  • 26 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea exl-1 35
  • 25 (a)
ManyToMany
exc-4 35
  • 21 (a)
ManyToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.6991 35
  • 46 (a)
OneToMany
Cin.2986 34
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.2986 34
Species where no ortholog for CLIC4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for CLIC4 Gene

ENSEMBL:
Gene Tree for CLIC4 (if available)
TreeFam:
Gene Tree for CLIC4 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for CLIC4: view image

Paralogs for CLIC4 Gene

(6) SIMAP similar genes for CLIC4 Gene using alignment to 2 proteins:

  • CLIC4_HUMAN
  • Q6FIC5_HUMAN

Pseudogenes.org Pseudogenes for CLIC4 Gene

genes like me logo Genes that share paralogs with CLIC4: view

Variants for CLIC4 Gene

Sequence variations from dbSNP and Humsavar for CLIC4 Gene

SNP ID Clin Chr 01 pos Variation AA Info Type
rs1000013824 -- 24,759,317(+) T/C intron_variant
rs1000014631 -- 24,764,577(+) C/A intron_variant
rs1000016406 -- 24,804,558(+) TGTGTGT/TGTGT intron_variant
rs1000195705 -- 24,765,573(+) G/C intron_variant
rs1000220400 -- 24,816,014(+) C/T intron_variant

Structural Variations from Database of Genomic Variants (DGV) for CLIC4 Gene

Variant ID Type Subtype PubMed ID
dgv2n73 CNV deletion 24416366
dgv63n106 CNV deletion 24896259
esv1446144 CNV deletion 17803354
esv2082154 CNV deletion 18987734
esv2525447 CNV deletion 19546169
esv2745530 CNV deletion 23290073
esv3559454 CNV deletion 23714750
esv3585512 CNV loss 21293372
esv3585513 CNV loss 21293372
esv3585514 CNV loss 21293372
esv3809 CNV loss 18987735
esv7429 CNV loss 19470904
esv995045 CNV deletion 20482838
esv995974 CNV deletion 20482838
nsv1002864 CNV loss 25217958
nsv1014245 CNV gain 25217958
nsv1076284 CNV deletion 25765185
nsv1113090 CNV deletion 24896259
nsv1121757 CNV deletion 24896259
nsv1151632 CNV deletion 26484159
nsv160319 CNV deletion 16902084
nsv498666 CNV loss 21111241
nsv511684 CNV loss 21212237
nsv522437 CNV gain 19592680
nsv7076 CNV deletion 18451855
nsv945829 CNV duplication 23825009

Variation tolerance for CLIC4 Gene

Residual Variation Intolerance Score: 25% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.26; 5.78% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for CLIC4 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
CLIC4

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CLIC4 Gene

Disorders for CLIC4 Gene

Additional Disease Information for CLIC4

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology

No disorders were found for CLIC4 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for CLIC4 Gene

Publications for CLIC4 Gene

  1. Chloride intracellular channel 4 is involved in endothelial proliferation and morphogenesis in vitro. (PMID: 19247789) Tung JJ … Kitajewski J (Angiogenesis 2009) 3 4 23 58
  2. Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of multiple human cancers. (PMID: 17200346) Suh KS … Yuspa SH (Clinical cancer research : an official journal of the American Association for Cancer Research 2007) 3 4 23 58
  3. Proteomic analysis of vascular endothelial growth factor-induced endothelial cell differentiation reveals a role for chloride intracellular channel 4 (CLIC4) in tubular morphogenesis. (PMID: 16239224) Bohman S … Claesson-Welsh L (The Journal of biological chemistry 2005) 3 4 23 58
  4. Differential expression of a chloride intracellular channel gene, CLIC4, in transforming growth factor-beta1-mediated conversion of fibroblasts to myofibroblasts. (PMID: 12163372) Rønnov-Jessen L … Petersen OW (The American journal of pathology 2002) 3 4 23 58
  5. Identification of a novel member of the chloride intracellular channel gene family (CLIC5) that associates with the actin cytoskeleton of placental microvilli. (PMID: 10793131) Berryman M … Bretscher A (Molecular biology of the cell 2000) 3 4 23 58

Products for CLIC4 Gene

Sources for CLIC4 Gene

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