This gene encodes an N-acetylglucosamine 6-O sulfotransferase. The encoded enzyme transfers sulfate from 3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. This protein is localized to the Golgi and is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. Alternate splicing i... See more...

Aliases for CHST4 Gene

Aliases for CHST4 Gene

  • Carbohydrate Sulfotransferase 4 2 3 4 5
  • LSST 2 3 4
  • Galactose/N-Acetylglucosamine/N-Acetylglucosamine 6-O-Sulfotransferase 3 3 4
  • High Endothelial Cells N-Acetylglucosamine 6-O-Sulfotransferase 3 4
  • Carbohydrate (N-Acetylglucosamine 6-O) Sulfotransferase 4 2 3
  • N-Acetylglucosamine 6-O-Sulfotransferase 2 3 4
  • L-Selectin Ligand Sulfotransferase 3 4
  • HEC-GlcNAc6ST 3 4
  • GlcNAc6ST-2 3 4
  • Gn6st-2 3 4
  • GST-3 3 4
  • Galactose/N-Acetylglucosamine/N-Acetylgalactosamine 6-O-Sulfotransferase 3 3
  • HEC-GLCNAC-6-ST 2
  • HECGLCNAC6ST 3
  • EC 2.8.2.21 51
  • EC 2.8.2.17 51
  • GlcNAc6ST2 3
  • EC 2.8.2.- 4
  • EC 2.8.2 51
  • CHST4 5
  • GST3 3

External Ids for CHST4 Gene

Previous GeneCards Identifiers for CHST4 Gene

  • GC16P062451
  • GC16P072283
  • GC16P071295
  • GC16P071336
  • GC16P070117
  • GC16P057328

Summaries for CHST4 Gene

Entrez Gene Summary for CHST4 Gene

  • This gene encodes an N-acetylglucosamine 6-O sulfotransferase. The encoded enzyme transfers sulfate from 3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. This protein is localized to the Golgi and is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2009]

GeneCards Summary for CHST4 Gene

CHST4 (Carbohydrate Sulfotransferase 4) is a Protein Coding gene. Diseases associated with CHST4 include Mucinous Adenocarcinoma and Cholangiolocellular Carcinoma. Among its related pathways are Keratan sulfate/keratin metabolism and Metabolism of proteins. Gene Ontology (GO) annotations related to this gene include sulfotransferase activity and N-acetylglucosamine 6-O-sulfotransferase activity. An important paralog of this gene is CHST6.

UniProtKB/Swiss-Prot Summary for CHST4 Gene

  • Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location.

Gene Wiki entry for CHST4 Gene

Additional gene information for CHST4 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for CHST4 Gene

Genomics for CHST4 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for CHST4 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH16J071525 Promoter/Enhancer 1.4 EPDnew Ensembl ENCODE 600.7 +1.0 1036 2.3 PRDM10 ZNF629 PRDM1 ZIC2 POLR2A ZNF600 JUND ZXDB OSR2 ZBTB48 CHST4 ZNF23 ENSG00000260015 ZNF19 lnc-TAT-6
GH16J071387 Enhancer 0.7 Ensembl ENCODE 4 -138.4 -138432 2.8 USF1 POLR2A ZNF384 TEAD4 SP1 ZNF148 CBFA2T3 CALB2 ZNF19 HPR ENSG00000260593 CHST4 LOC105371332 LINC02136 TLE7
GH16J071515 Enhancer 0.8 Ensembl ENCODE 0.4 -10.1 -10059 0.3 REST SP1 FOXA2 YY1 GABPA ZBTB33 JUND NR2F2 RAD21 HNF4A ENSG00000260520 LOC105371335 ZNF23 CHST4 ENSG00000261611 ZNF19 lnc-TAT-6
GH16J071492 Enhancer 1 Ensembl ENCODE 0.3 -32.9 -32932 5.8 LEF1 CTCF JUND MTA2 PKNOX1 ZNF24 DPF2 HDAC1 SOX6 FOXJ2 ZNF23 SF3B3 DHX38 ZNF19 ENSG00000247324 TAT CALB2 VAC14 TAT-AS1 PHLPP2
GH16J071537 Enhancer 0.6 ENCODE 0.4 +12.6 12640 0.2 CTCF REST RAD21 TRIM22 SCRT2 ZNF444 SMC3 SCRT1 DEK BATF ENSG00000260015 CHST4 ZNF19 lnc-TAT-6
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around CHST4 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for CHST4

Top Transcription factor binding sites by QIAGEN in the CHST4 gene promoter:
  • Evi-1
  • FOXJ2
  • FOXJ2 (long isoform)
  • GATA-3
  • GR
  • GR-alpha
  • GR-beta
  • HFH-1
  • NF-E2
  • NF-E2 p45

Genomic Locations for CHST4 Gene

Genomic Locations for CHST4 Gene
chr16:71,525,233-71,538,748
(GRCh38/hg38)
Size:
13,516 bases
Orientation:
Plus strand
chr16:71,559,136-71,572,649
(GRCh37/hg19)
Size:
13,514 bases
Orientation:
Plus strand

Genomic View for CHST4 Gene

Genes around CHST4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CHST4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CHST4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CHST4 Gene

Proteins for CHST4 Gene

  • Protein details for CHST4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8NCG5-CHST4_HUMAN
    Recommended name:
    Carbohydrate sulfotransferase 4
    Protein Accession:
    Q8NCG5
    Secondary Accessions:
    • Q8IV46
    • Q9Y5R3

    Protein attributes for CHST4 Gene

    Size:
    386 amino acids
    Molecular mass:
    45134 Da
    Quaternary structure:
    • Monomer.
    SequenceCaution:
    • Sequence=AAH35282.1; Type=Erroneous initiation; Evidence={ECO:0000305};
    Miscellaneous:
    • May serve as an antiinflammatory target.

neXtProt entry for CHST4 Gene

Selected DME Specific Peptides for CHST4 Gene

Q8NCG5:
  • RSGSSFVGQLF
  • QHPDVFYLMEPAWHVW
  • LVRDPRAV
  • LHMAVRDL
  • VVLKEVRF

Post-translational modifications for CHST4 Gene

  • Glycosylation at Asn30, Asn308, and Asn329
  • Modification sites at PhosphoSitePlus

Other Protein References for CHST4 Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for CHST4 Gene

Gene Families for CHST4 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for CHST4 Gene

Suggested Antigen Peptide Sequences for CHST4 Gene

GenScript: Design optimal peptide antigens:
  • N-acetylglucosamine 6-O-sulfotransferase 2 (CHST4_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q8NCG5

UniProtKB/Swiss-Prot:

CHST4_HUMAN :
  • Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
Family:
  • Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
genes like me logo Genes that share domains with CHST4: view

Function for CHST4 Gene

Molecular function for CHST4 Gene

UniProtKB/Swiss-Prot Function:
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location.
UniProtKB/Swiss-Prot Induction:
Upon cytokine activation, it is expressed at low level.

Enzyme Numbers (IUBMB) for CHST4 Gene

Gene Ontology (GO) - Molecular Function for CHST4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001517 N-acetylglucosamine 6-O-sulfotransferase activity TAS --
GO:0008146 sulfotransferase activity TAS 10330415
GO:0016740 transferase activity IEA --
genes like me logo Genes that share ontologies with CHST4: view
genes like me logo Genes that share phenotypes with CHST4: view

Animal Models for CHST4 Gene

MGI Knock Outs for CHST4:

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CHST4

No data available for Phenotypes From GWAS Catalog , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for CHST4 Gene

Localization for CHST4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CHST4 Gene

Golgi apparatus membrane. Single-pass type II membrane protein.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CHST4 gene
Compartment Confidence
golgi apparatus 5
extracellular 3
plasma membrane 1
cytoskeleton 1
nucleus 1
endoplasmic reticulum 1
mitochondrion 0
peroxisome 0
lysosome 0

Gene Ontology (GO) - Cellular Components for CHST4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane TAS --
GO:0005794 Golgi apparatus IEA --
GO:0005802 trans-Golgi network IBA,IDA 12855678
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane IEA --
genes like me logo Genes that share ontologies with CHST4: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for CHST4 Gene

Pathways & Interactions for CHST4 Gene

genes like me logo Genes that share pathways with CHST4: view

Pathways by source for CHST4 Gene

Interacting Proteins for CHST4 Gene

Gene Ontology (GO) - Biological Process for CHST4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005975 carbohydrate metabolic process IEA --
GO:0006044 N-acetylglucosamine metabolic process IDA 10330415
GO:0006477 protein sulfation IBA 21873635
GO:0006790 sulfur compound metabolic process IBA,IDA 10330415
GO:0006954 inflammatory response IEA --
genes like me logo Genes that share ontologies with CHST4: view

No data available for SIGNOR curated interactions for CHST4 Gene

Drugs & Compounds for CHST4 Gene

(1) Drugs for CHST4 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
genes like me logo Genes that share compounds with CHST4: view

Transcripts for CHST4 Gene

mRNA/cDNA for CHST4 Gene

2 REFSEQ mRNAs :
9 NCBI additional mRNA sequence :
3 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CHST4

Alternative Splicing Database (ASD) splice patterns (SP) for CHST4 Gene

No ASD Table

Relevant External Links for CHST4 Gene

GeneLoc Exon Structure for
CHST4

Expression for CHST4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for CHST4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for CHST4 Gene

This gene is overexpressed in Pancreas (x19.4), Liver (x12.2), and Adrenal Gland (x4.6).

Protein differential expression in normal tissues from HIPED for CHST4 Gene

This gene is overexpressed in Nasal epithelium (57.1) and Gallbladder (9.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for CHST4 Gene



Protein tissue co-expression partners for CHST4 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for CHST4

SOURCE GeneReport for Unigene cluster for CHST4 Gene:

Hs.251383

mRNA Expression by UniProt/SwissProt for CHST4 Gene:

Q8NCG5-CHST4_HUMAN
Tissue specificity: Specifically expressed in HEV. Weakly expressed in spleen. Not expressed in other tissues. Expressed in colonic mucinous adenocarcinoma.

Evidence on tissue expression from TISSUES for CHST4 Gene

  • Nervous system(4.3)
  • Gall bladder(2.1)
genes like me logo Genes that share expression patterns with CHST4: view

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for CHST4 Gene

Orthologs for CHST4 Gene

This gene was present in the common ancestor of animals.

Orthologs for CHST4 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia CHST4 30 31
  • 99.48 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia CHST4 30 31
  • 84.74 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia CHST4 30 31
  • 82.9 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Chst4 30
  • 78.14 (n)
Mouse
(Mus musculus)
Mammalia Chst4 30 17 31
  • 77.96 (n)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia CHST4 31
  • 64 (a)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia -- 31
  • 62 (a)
OneToMany
-- 31
  • 62 (a)
OneToMany
Chicken
(Gallus gallus)
Aves CHST4 30
  • 63.83 (n)
-- 31
  • 51 (a)
ManyToMany
Lizard
(Anolis carolinensis)
Reptilia CHST4 31
  • 55 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia LOC100496630 30
  • 57.29 (n)
Zebrafish
(Danio rerio)
Actinopterygii CHST5 31
  • 49 (a)
OneToMany
Fruit Fly
(Drosophila melanogaster)
Insecta CG9550 31
  • 19 (a)
ManyToMany
CG31637 31
  • 17 (a)
ManyToMany
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 31 (a)
ManyToMany
-- 31
  • 28 (a)
ManyToMany
-- 31
  • 25 (a)
ManyToMany
-- 31
  • 23 (a)
ManyToMany
-- 31
  • 22 (a)
ManyToMany
-- 31
  • 20 (a)
ManyToMany
CSA.5535 31
  • 18 (a)
ManyToMany
-- 31
  • 17 (a)
ManyToMany
Species where no ortholog for CHST4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for CHST4 Gene

ENSEMBL:
Gene Tree for CHST4 (if available)
TreeFam:
Gene Tree for CHST4 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for CHST4: view image

Paralogs for CHST4 Gene

Paralogs for CHST4 Gene

(6) SIMAP similar genes for CHST4 Gene using alignment to 1 proteins:

  • CHST4_HUMAN
genes like me logo Genes that share paralogs with CHST4: view

Variants for CHST4 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for CHST4 Gene

SNP ID Clinical significance and condition Chr 16 pos Variation AA Info Type
rs3813744 - p.His361Gln

Additional dbSNP identifiers (rs#s) for CHST4 Gene

Structural Variations from Database of Genomic Variants (DGV) for CHST4 Gene

Variant ID Type Subtype PubMed ID
nsv7285 OTHER inversion 18451855

Variation tolerance for CHST4 Gene

Residual Variation Intolerance Score: 56.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.88; 35.20% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for CHST4 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
CHST4

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CHST4 Gene

Disorders for CHST4 Gene

MalaCards: The human disease database

(3) MalaCards diseases for CHST4 Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
mucinous adenocarcinoma
  • mucin-producing adenocarcinoma
cholangiolocellular carcinoma
colon mucinous adenocarcinoma
  • colonic mucinous adenocarcinoma
- elite association - COSMIC cancer census association via MalaCards
Search CHST4 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for CHST4

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
genes like me logo Genes that share disorders with CHST4: view

No data available for UniProtKB/Swiss-Prot and Genatlas for CHST4 Gene

Publications for CHST4 Gene

  1. Specificities of N-acetylglucosamine-6-O-sulfotransferases in relation to L-selectin ligand synthesis and tumor-associated enzyme expression. (PMID: 11726653) Uchimura K … Muramatsu T (The Journal of biological chemistry 2002) 3 4 23
  2. CHST1 and CHST2 sulfotransferase expression by vascular endothelial cells regulates shear-resistant leukocyte rolling via L-selectin. (PMID: 11310842) Li X … Tedder TF (Journal of leukocyte biology 2001) 3 4 23
  3. Sulfotransferases of two specificities function in the reconstitution of high endothelial cell ligands for L-selectin. (PMID: 10330415) Bistrup A … Hemmerich S (The Journal of cell biology 1999) 2 3 4
  4. Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects. (PMID: 19343046) Saito A … Kamatani N (Journal of human genetics 2009) 3 41
  5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4

Products for CHST4 Gene

Sources for CHST4 Gene