This locus encodes a member of the keratin sulfotransferase family of proteins. The encoded enzyme catalyzes the sulfation of the proteoglycan keratin. [provided by RefSeq, Aug 2011] See more...

Aliases for CHST1 Gene

Aliases for CHST1 Gene

  • Carbohydrate Sulfotransferase 1 2 3 4 5
  • KSGal6ST 2 3 4
  • Galactose/N-Acetylglucosamine/N-Acetylglucosamine 6-O-Sulfotransferase 1 3 4
  • Carbohydrate (Keratan Sulfate Gal-6) Sulfotransferase 1 2 3
  • Keratan Sulfotransferase 2 3
  • EC 2.8.2.21 4 51
  • GST-1 3 4
  • KS6ST 3 4
  • C6ST 2 3
  • KSST 3 4
  • Carbohydrate (Chondroitin 6/Keratan) Sulfotransferase 1 3
  • Keratan Sulfate Gal-6 Sulfotransferase 4
  • EC 2.8.2 51
  • CHST1 5

External Ids for CHST1 Gene

Previous GeneCards Identifiers for CHST1 Gene

  • GC11M047454
  • GC11M046360
  • GC11M045702
  • GC11M045634
  • GC11M045627
  • GC11M045378
  • GC11M045670

Summaries for CHST1 Gene

Entrez Gene Summary for CHST1 Gene

  • This locus encodes a member of the keratin sulfotransferase family of proteins. The encoded enzyme catalyzes the sulfation of the proteoglycan keratin. [provided by RefSeq, Aug 2011]

GeneCards Summary for CHST1 Gene

CHST1 (Carbohydrate Sulfotransferase 1) is a Protein Coding gene. Among its related pathways are Keratan sulfate/keratin metabolism and Cytochrome P450 - arranged by substrate type. Gene Ontology (GO) annotations related to this gene include sulfotransferase activity and keratan sulfotransferase activity. An important paralog of this gene is CHST3.

UniProtKB/Swiss-Prot Summary for CHST1 Gene

  • Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation.

Gene Wiki entry for CHST1 Gene

Additional gene information for CHST1 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for CHST1 Gene

Genomics for CHST1 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for CHST1 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around CHST1 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for CHST1

Top Transcription factor binding sites by QIAGEN in the CHST1 gene promoter:
  • AREB6
  • C/EBPbeta
  • HOXA5
  • LCR-F1
  • Lhx3a
  • LHX3b
  • Max
  • NF-kappaB
  • NF-kappaB1
  • NF-kappaB2

Genomic Locations for CHST1 Gene

Genomic Locations for CHST1 Gene
chr11:45,647,689-45,665,651
(GRCh38/hg38)
Size:
17,963 bases
Orientation:
Minus strand
chr11:45,670,427-45,687,172
(GRCh37/hg19)
Size:
16,746 bases
Orientation:
Minus strand

Genomic View for CHST1 Gene

Genes around CHST1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CHST1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CHST1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CHST1 Gene

Proteins for CHST1 Gene

  • Protein details for CHST1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O43916-CHST1_HUMAN
    Recommended name:
    Carbohydrate sulfotransferase 1
    Protein Accession:
    O43916
    Secondary Accessions:
    • D3DQP2

    Protein attributes for CHST1 Gene

    Size:
    411 amino acids
    Molecular mass:
    46715 Da
    Quaternary structure:
    No Data Available

neXtProt entry for CHST1 Gene

Selected DME Specific Peptides for CHST1 Gene

O43916:
  • ETFRDTYRLWR
  • SIAIQYTAIRTFTAK
  • EDPRLNLKVIQLVRDPRGIL
  • YMLVRYED
  • DVFYLFEPLYHVQ
  • CDLYFLE
  • TGRKPYNLD
  • RSGSSFVGQLF
  • YMLVRYEDLARNP
  • LVEDPRL
  • RRVMLGASRDLLRSLY

Post-translational modifications for CHST1 Gene

  • Glycosylation at Asn56, Asn145, Asn189, and Asn334
  • Modification sites at PhosphoSitePlus

Other Protein References for CHST1 Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for CHST1 Gene

Gene Families for CHST1 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins

Protein Domains for CHST1 Gene

Suggested Antigen Peptide Sequences for CHST1 Gene

GenScript: Design optimal peptide antigens:
  • Keratan sulfate Gal-6 sulfotransferase (CHST1_HUMAN)

Graphical View of Domain Structure for InterPro Entry

O43916

UniProtKB/Swiss-Prot:

CHST1_HUMAN :
  • Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
Family:
  • Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.
genes like me logo Genes that share domains with CHST1: view

Function for CHST1 Gene

Molecular function for CHST1 Gene

UniProtKB/Swiss-Prot Function:
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=3'-phosphoadenylyl sulfate + keratan = adenosine 3',5'-bisphosphate + keratan 6'-sulfate.; EC=2.8.2.21; Evidence={ECO:0000269|PubMed:9405439};.
GENATLAS Biochemistry:
chondroitin 6-sulfotransferase key enzyme 1,expressed in vascular endothelial cells,implicated in chondrogenesis,neoplasia,atherosclerosis and other

Enzyme Numbers (IUBMB) for CHST1 Gene

Phenotypes From GWAS Catalog for CHST1 Gene

Gene Ontology (GO) - Molecular Function for CHST1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001517 N-acetylglucosamine 6-O-sulfotransferase activity IBA 21873635
GO:0008146 sulfotransferase activity IDA 10330415
GO:0016740 transferase activity IEA --
GO:0045130 keratan sulfotransferase activity TAS --
genes like me logo Genes that share ontologies with CHST1: view
genes like me logo Genes that share phenotypes with CHST1: view

Animal Models for CHST1 Gene

MGI Knock Outs for CHST1:
  • Chst1 Chst1<tm1(KOMP)Vlcg>

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CHST1

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for CHST1 Gene

Localization for CHST1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CHST1 Gene

Golgi apparatus membrane. Single-pass type II membrane protein.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CHST1 gene
Compartment Confidence
golgi apparatus 4
extracellular 3
plasma membrane 1
cytoskeleton 1
nucleus 1
endoplasmic reticulum 1
cytosol 1
lysosome 1
mitochondrion 0

Gene Ontology (GO) - Cellular Components for CHST1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane TAS --
GO:0005794 Golgi apparatus IEA --
GO:0005802 trans-Golgi network IBA 21873635
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane TAS,NAS 9405439
genes like me logo Genes that share ontologies with CHST1: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for CHST1 Gene

Pathways & Interactions for CHST1 Gene

genes like me logo Genes that share pathways with CHST1: view

Pathways by source for CHST1 Gene

Gene Ontology (GO) - Biological Process for CHST1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005975 carbohydrate metabolic process IEA --
GO:0005976 polysaccharide metabolic process TAS 9405439
GO:0006012 galactose metabolic process IDA 10330415
GO:0006044 N-acetylglucosamine metabolic process IBA 21873635
GO:0006790 sulfur compound metabolic process IBA,IDA 10330415
genes like me logo Genes that share ontologies with CHST1: view

No data available for SIGNOR curated interactions for CHST1 Gene

Drugs & Compounds for CHST1 Gene

(7) Drugs for CHST1 Gene - From: PharmGKB, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
imatinib Approved Pharma Kinase Inhibitors, SRC/BCR-ABL tyrosine kinase inhibitors 0
3'-phospho-5'-adenylyl sulfate Experimental Pharma 0
Adenosine 3',5'-diphosphate Experimental Pharma 0

(5) Additional Compounds for CHST1 Gene - From: HMDB and Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Keratan
  • Keratan 6'-sulfate
  • Keratan 6'-sulphate
  • Keratan sulfate
  • Keratan sulphate
  • kerato 6'-Sulfate
69992-87-6
n-acetyllactosamine
  • beta-D-Galactosyl-1,4-N-acetyl-D-glucosamine
  • Galb1-4glcnacb
  • Galbeta1-4glcnacbeta
  • LacNAc
  • b-D-Galactosyl-1,4-N-acetyl-D-glucosamine
32181-59-2
genes like me logo Genes that share compounds with CHST1: view

Transcripts for CHST1 Gene

mRNA/cDNA for CHST1 Gene

1 REFSEQ mRNAs :
10 NCBI additional mRNA sequence :
3 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CHST1

Alternative Splicing Database (ASD) splice patterns (SP) for CHST1 Gene

No ASD Table

Relevant External Links for CHST1 Gene

GeneLoc Exon Structure for
CHST1

Expression for CHST1 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for CHST1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for CHST1 Gene

This gene is overexpressed in Brain - Cortex (x5.6), Brain - Frontal Cortex (BA9) (x5.0), Brain - Anterior cingulate cortex (BA24) (x4.6), and Brain - Amygdala (x4.0).

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for CHST1

SOURCE GeneReport for Unigene cluster for CHST1 Gene:

Hs.104576

mRNA Expression by UniProt/SwissProt for CHST1 Gene:

O43916-CHST1_HUMAN
Tissue specificity: Widely expressed at low level. Expressed in brain and skeletal muscle. Expressed by high endothelial cells (HEVs) and leukocytes.

Evidence on tissue expression from TISSUES for CHST1 Gene

  • Nervous system(4.7)
  • Heart(4.2)
genes like me logo Genes that share expression patterns with CHST1: view

No data available for Protein differential expression in normal tissues , Protein expression , Protein tissue co-expression partners and Phenotype-based relationships between genes and organs from Gene ORGANizer for CHST1 Gene

Orthologs for CHST1 Gene

This gene was present in the common ancestor of animals.

Orthologs for CHST1 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia CHST1 30 31
  • 99.59 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia CHST1 30 31
  • 93.67 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia CHST1 30 31
  • 92.86 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Chst1 30
  • 87.8 (n)
Oppossum
(Monodelphis domestica)
Mammalia CHST1 31
  • 87 (a)
OneToOne
Mouse
(Mus musculus)
Mammalia Chst1 30 17 31
  • 86.67 (n)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia CHST1 31
  • 71 (a)
OneToOne
Chicken
(Gallus gallus)
Aves CHST1 30 31
  • 82.4 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia CHST1 31
  • 81 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia chst1 30
  • 66.26 (n)
Zebrafish
(Danio rerio)
Actinopterygii chst1 30 31
  • 66.99 (n)
OneToOne
Fruit Fly
(Drosophila melanogaster)
Insecta CG9550 31
  • 20 (a)
ManyToMany
CG31637 31
  • 18 (a)
ManyToMany
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 37 (a)
ManyToMany
-- 31
  • 30 (a)
ManyToMany
-- 31
  • 27 (a)
ManyToMany
-- 31
  • 24 (a)
ManyToMany
-- 31
  • 24 (a)
ManyToMany
-- 31
  • 22 (a)
ManyToMany
CSA.5535 31
  • 21 (a)
ManyToMany
-- 31
  • 21 (a)
ManyToMany
Species where no ortholog for CHST1 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for CHST1 Gene

ENSEMBL:
Gene Tree for CHST1 (if available)
TreeFam:
Gene Tree for CHST1 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for CHST1: view image

Paralogs for CHST1 Gene

Paralogs for CHST1 Gene

(4) SIMAP similar genes for CHST1 Gene using alignment to 1 proteins:

  • CHST1_HUMAN
genes like me logo Genes that share paralogs with CHST1: view

Variants for CHST1 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for CHST1 Gene

SNP ID Clinical significance and condition Chr 11 pos Variation AA Info Type
691422 Uncertain Significance: Pyloric stenosis; Esophageal atresia 45,649,909(-) C/T MISSENSE_VARIANT

Additional dbSNP identifiers (rs#s) for CHST1 Gene

Structural Variations from Database of Genomic Variants (DGV) for CHST1 Gene

Variant ID Type Subtype PubMed ID
esv24804 CNV loss 19812545
nsv554197 CNV loss 21841781
nsv554198 CNV loss 21841781
nsv554199 CNV loss 21841781

Variation tolerance for CHST1 Gene

Residual Variation Intolerance Score: 12.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.17; 23.61% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for CHST1 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
CHST1

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CHST1 Gene

Disorders for CHST1 Gene

Additional Disease Information for CHST1

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform

No disorders were found for CHST1 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for CHST1 Gene

Publications for CHST1 Gene

  1. Human chondroitin 6-sulfotransferase: cloning, gene structure, and chromosomal localization. (PMID: 9639683) Mazany KD … Williams KJ (Biochimica et biophysica acta 1998) 2 3 4 23
  2. Molecular cloning and characterization of human keratan sulfate Gal-6-sulfotransferase. (PMID: 9405439) Fukuta M … Habuchi O (The Journal of biological chemistry 1997) 2 3 4 23
  3. CHST1 and CHST2 sulfotransferase expression by vascular endothelial cells regulates shear-resistant leukocyte rolling via L-selectin. (PMID: 11310842) Li X … Tedder TF (Journal of leukocyte biology 2001) 3 4 23
  4. Sulfation of sialyl N-acetyllactosamine oligosaccharides and fetuin oligosaccharides by keratan sulfate Gal-6-sulfotransferase. (PMID: 10642612) Torii T … Habuchi O (Glycobiology 2000) 3 4 23
  5. CHST1 and CHST2 sulfotransferases expressed by human vascular endothelial cells: cDNA cloning, expression, and chromosomal localization. (PMID: 10049591) Li X … Tedder TF (Genomics 1999) 3 4 23

Products for CHST1 Gene

Sources for CHST1 Gene