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Aliases for CHRM3 Gene

Aliases for CHRM3 Gene

  • Cholinergic Receptor Muscarinic 3 2 3 5
  • Acetylcholine Receptor, Muscarinic 3 2 3
  • Muscarinic Acetylcholine Receptor M3 3
  • M3 Muscarinic Receptor 3
  • EGBRS 3
  • HM3 3
  • PBS 3

External Ids for CHRM3 Gene

Previous GeneCards Identifiers for CHRM3 Gene

  • GC01P238144
  • GC01P235523
  • GC01P235846
  • GC01P237111
  • GC01P236396
  • GC01P237858
  • GC01P239549
  • GC01P210250

Summaries for CHRM3 Gene

Entrez Gene Summary for CHRM3 Gene

  • The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016]

GeneCards Summary for CHRM3 Gene

CHRM3 (Cholinergic Receptor Muscarinic 3) is a Protein Coding gene. Diseases associated with CHRM3 include Prune Belly Syndrome and Cholinergic Urticaria. Among its related pathways are GPCRs, Other and Monoamine GPCRs. Gene Ontology (GO) annotations related to this gene include G-protein coupled receptor activity and drug binding. An important paralog of this gene is CHRM5.

UniProtKB/Swiss-Prot for CHRM3 Gene

  • The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Tocris Summary for CHRM3 Gene

  • Muscarinic receptors are widely distributed throughout the body and control distinct functions according to location and subtype (M1 - M5).They are predominantly expressed in the parasympathetic nervous system where they exert both inhibitory and excitatory effects.

Gene Wiki entry for CHRM3 Gene

Additional gene information for CHRM3 Gene

No data available for CIViC summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CHRM3 Gene

Genomics for CHRM3 Gene

GeneHancer (GH) Regulatory Elements for CHRM3 Gene

Promoters and enhancers for CHRM3 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH01J239386 Enhancer 0.7 ENCODE 650.7 +0.6 580 2.2 CTCF USF1 SUZ12 MAX ZIC2 HIC1 ZNF335 ZFHX2 ZNF791 ZNF138 CHRM3 GC01M239442
GH01J239771 Promoter 0.5 EPDnew 650.1 +384.9 384897 0.1 CHRM3 CHRM3-AS2 CHRM3-AS1
GH01J239717 Enhancer 1 Ensembl ENCODE dbSUPER 11.3 +333.2 333225 5.3 GATA3 POLR2A MAX RCOR1 REST MYC GABPA TRIM28 CHRM3-AS2 CHRM3 GC01P239706
GH01J239814 Enhancer 0.9 Ensembl ENCODE 11 +428.0 428006 0.9 CTCF JUN MAX BACH1 SIN3A FEZF1 RAD21 RFX5 SMC3 SMARCC2 CHRM3 CHRM3-AS2 CHRM3-AS1
GH01J239361 Enhancer 0.4 FANTOM5 17.3 -25.0 -24983 0.4 ZFHX2 EGR2 CHRM3 LOC105373223
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around CHRM3 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the CHRM3 gene promoter:
  • AP-2alphaA
  • AP-2alpha isoform 4
  • AP-2alpha isoform 3
  • AP-2alpha isoform 2
  • AP-2alpha
  • HTF
  • FOXJ2
  • FOXJ2 (long isoform)
  • FOXO4
  • POU2F1a

Genomic Locations for CHRM3 Gene

Genomic Locations for CHRM3 Gene
528,888 bases
Plus strand
528,886 bases
Plus strand

Genomic View for CHRM3 Gene

Genes around CHRM3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CHRM3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CHRM3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CHRM3 Gene

Proteins for CHRM3 Gene

  • Protein details for CHRM3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Muscarinic acetylcholine receptor M3
    Protein Accession:
    Secondary Accessions:
    • Q0VAJ8
    • Q4QRI3
    • Q5VXY2
    • Q9HB60

    Protein attributes for CHRM3 Gene

    590 amino acids
    Molecular mass:
    66128 Da
    Quaternary structure:
    • Homodimer; the dimers can form tetramers (PubMed:23521066). Interacts with NALCN (By similarity).

    Three dimensional structures from OCA and Proteopedia for CHRM3 Gene

neXtProt entry for CHRM3 Gene

Post-translational modifications for CHRM3 Gene

  • Glycosylation at posLast=4848, Asn41, Asn15, Asn6, and posLast=55
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for CHRM3 Gene

Domains & Families for CHRM3 Gene

Gene Families for CHRM3 Gene

Human Protein Atlas (HPA):
  • Disease related genes
  • FDA approved drug targets
  • G-protein coupled receptors
  • Predicted membrane proteins

Suggested Antigen Peptide Sequences for CHRM3 Gene

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.
  • Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.
genes like me logo Genes that share domains with CHRM3: view

Function for CHRM3 Gene

Molecular function for CHRM3 Gene

UniProtKB/Swiss-Prot Function:
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
GENATLAS Biochemistry:
cholinergic receptor,muscarinic 3,G protein coupled receptor superfamily

Phenotypes From GWAS Catalog for CHRM3 Gene

Gene Ontology (GO) - Molecular Function for CHRM3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004435 phosphatidylinositol phospholipase C activity TAS 1905013
GO:0004871 obsolete signal transducer activity IEA --
GO:0004930 G-protein coupled receptor activity IEA --
GO:0005515 protein binding IPI 19575010
GO:0008144 drug binding IEA --
genes like me logo Genes that share ontologies with CHRM3: view
genes like me logo Genes that share phenotypes with CHRM3: view

Human Phenotype Ontology for CHRM3 Gene

HPO Id HPO Name Alternative Ids Definition Synonyms

Animal Models for CHRM3 Gene

MGI Knock Outs for CHRM3:

Animal Model Products

miRNA for CHRM3 Gene

miRTarBase miRNAs that target CHRM3

Clone Products

  • Addgene plasmids for CHRM3

No data available for Enzyme Numbers (IUBMB) , Transcription Factor Targets and HOMER Transcription for CHRM3 Gene

Localization for CHRM3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CHRM3 Gene

Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CHRM3 gene
Compartment Confidence
plasma membrane 5
nucleus 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Plasma membrane (3)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for CHRM3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005886 plasma membrane TAS --
GO:0005887 integral component of plasma membrane TAS,ISS --
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane IEA --
GO:0016323 basolateral plasma membrane IEA --
genes like me logo Genes that share ontologies with CHRM3: view

Pathways & Interactions for CHRM3 Gene

genes like me logo Genes that share pathways with CHRM3: view

Gene Ontology (GO) - Biological Process for CHRM3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003056 regulation of vascular smooth muscle contraction IBA --
GO:0006464 cellular protein modification process TAS 10051746
GO:0006939 smooth muscle contraction IEA --
GO:0007165 signal transduction TAS 10940357
GO:0007186 G-protein coupled receptor signaling pathway TAS --
genes like me logo Genes that share ontologies with CHRM3: view

No data available for SIGNOR curated interactions for CHRM3 Gene

Drugs & Compounds for CHRM3 Gene

(220) Drugs for CHRM3 Gene - From: DrugBank, DGIdb, FDA Approved Drugs, IUPHAR, HMDB, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Atropine Approved, Vet_approved Pharma Antagonist, antagonist, Target MAChRs antagonist 153
Darifenacin Approved, Investigational Pharma Antagonist, Inverse agonist, antagonist, Target M3 receptor antagonist and antimuscarinic agents 22
Cevimeline Approved Pharma agonist, Target Muscarinic receptor agonist 5
Ipratropium Approved Pharma Antagonist, antagonist, Target 146
Pilocarpine Approved, Investigational Pharma Partial agonist, Agonist, agonist, Target 27

(24) Additional Compounds for CHRM3 Gene - From: HMDB, IUPHAR, and Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Partial agonist, Agonist
Full agonist, Agonist
Gouml 7874
Neutral, Allosteric regulator, Negative
N-Dealkylated tolterodine

(4) Tocris Compounds for CHRM3 Gene

Compound Action Cas Number
4-DAMP Muscarinic M3 antagonist 1952-15-4
DAU 5884 hydrochloride M3 receptor antagonist 131780-48-8
J 104129 fumarate Potent, selective M3 antagonist 257603-40-0
Zamifenacin fumarate Selective M3 antagonist 127308-98-9
genes like me logo Genes that share compounds with CHRM3: view

Transcripts for CHRM3 Gene

Unigene Clusters for CHRM3 Gene

Cholinergic receptor, muscarinic 3:
Representative Sequences:

Clone Products

  • Addgene plasmids for CHRM3

Alternative Splicing Database (ASD) splice patterns (SP) for CHRM3 Gene

No ASD Table

Relevant External Links for CHRM3 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for CHRM3 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for CHRM3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for CHRM3 Gene

This gene is overexpressed in Pancreas (x4.9), Esophagus - Muscularis (x4.7), and Bladder (x4.0).

Protein differential expression in normal tissues from HIPED for CHRM3 Gene

This gene is overexpressed in Heart (21.4), Retina (19.7), Pancreas (15.8), and Frontal cortex (12.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for CHRM3 Gene

Protein tissue co-expression partners for CHRM3 Gene

NURSA nuclear receptor signaling pathways regulating expression of CHRM3 Gene:


SOURCE GeneReport for Unigene cluster for CHRM3 Gene:


Evidence on tissue expression from TISSUES for CHRM3 Gene

  • Nervous system(4.6)
  • Kidney(2.2)

Phenotype-based relationships between genes and organs from Gene ORGANizer for CHRM3 Gene

Germ Layers:
  • ectoderm
  • endoderm
  • mesoderm
  • cardiovascular
  • digestive
  • immune
  • integumentary
  • reproductive
  • respiratory
  • skeletal muscle
  • skeleton
  • urinary
Head and neck:
  • ear
  • head
  • jaw
  • mandible
  • maxilla
  • mouth
  • salivary gland
  • skull
  • tongue
  • chest wall
  • heart
  • lung
  • rib
  • rib cage
  • sternum
  • abdominal wall
  • intestine
  • kidney
  • large intestine
  • anus
  • pelvis
  • placenta
  • rectum
  • testicle
  • ureter
  • urethra
  • urinary bladder
  • uterus
  • femur
  • foot
  • hip
  • lower limb
  • thigh
  • blood
  • blood vessel
  • skin
  • white blood cell
genes like me logo Genes that share expression patterns with CHRM3: view

No data available for mRNA Expression by UniProt/SwissProt for CHRM3 Gene

Orthologs for CHRM3 Gene

This gene was present in the common ancestor of animals.

Orthologs for CHRM3 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia CHRM3 34 33
  • 99.6 (n)
(Bos Taurus)
Mammalia CHRM3 34 33
  • 90.51 (n)
(Canis familiaris)
Mammalia CHRM3 34 33
  • 88.34 (n)
(Rattus norvegicus)
Mammalia Chrm3 33
  • 87.61 (n)
(Mus musculus)
Mammalia Chrm3 16 34 33
  • 87.04 (n)
(Monodelphis domestica)
Mammalia CHRM3 34
  • 87 (a)
(Ornithorhynchus anatinus)
Mammalia CHRM3 34
  • 83 (a)
(Gallus gallus)
Aves CHRM3 34 33
  • 76.6 (n)
(Anolis carolinensis)
Reptilia CHRM3 34
  • 76 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia LOC100493547 33
  • 65.04 (n)
(Danio rerio)
Actinopterygii chrm3a 34 33
  • 64.77 (n)
chrm3b 34
  • 61 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG7918 35
  • 36 (a)
mAcR-60C 34 35
  • 27 (a)
(Caenorhabditis elegans)
Secernentea gar-3 34
  • 32 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 34
  • 40 (a)
Species where no ortholog for CHRM3 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for CHRM3 Gene

Gene Tree for CHRM3 (if available)
Gene Tree for CHRM3 (if available)
Evolutionary constrained regions (ECRs) for CHRM3: view image

Paralogs for CHRM3 Gene

Paralogs for CHRM3 Gene

(7) SIMAP similar genes for CHRM3 Gene using alignment to 4 proteins:

  • B1AN12_HUMAN
  • Q8NG01_HUMAN
  • Q96RG9_HUMAN
genes like me logo Genes that share paralogs with CHRM3: view

Variants for CHRM3 Gene

Sequence variations from dbSNP and Humsavar for CHRM3 Gene

SNP ID Clin Chr 01 pos Variation AA Info Type
rs587776862 pathogenic, Prune belly syndrome 239,908,624(+) GCCTGAGGAGGA/T coding_sequence_variant, frameshift
rs1000010892 -- 239,586,744(+) C/A genic_upstream_transcript_variant, intron_variant
rs1000011803 -- 239,771,504(+) T/C genic_upstream_transcript_variant, intron_variant
rs1000013746 -- 239,676,111(+) A/G genic_upstream_transcript_variant, intron_variant
rs1000014114 -- 239,643,431(+) C/T genic_upstream_transcript_variant, intron_variant

Structural Variations from Database of Genomic Variants (DGV) for CHRM3 Gene

Variant ID Type Subtype PubMed ID
dgv81e214 CNV gain 21293372
esv1778820 CNV insertion 17803354
esv2376547 CNV deletion 18987734
esv2725851 CNV deletion 23290073
esv2725862 CNV deletion 23290073
esv2759009 CNV gain 17122850
esv3373255 CNV insertion 20981092
esv35011 CNV gain 17911159
esv3589279 CNV loss 21293372
esv3589280 CNV loss 21293372
esv3589282 CNV gain 21293372
esv3589283 CNV gain 21293372
esv3589284 CNV gain 21293372
esv3589285 CNV gain 21293372
esv3891259 CNV gain 25118596
esv5899 CNV gain 19470904
nsv1127068 CNV deletion 24896259
nsv5065 CNV insertion 18451855
nsv506986 OTHER sequence alteration 20534489
nsv508067 OTHER sequence alteration 20534489
nsv519393 CNV gain 19592680
nsv549435 CNV gain 21841781
nsv549438 CNV gain 21841781
nsv549439 CNV loss 21841781
nsv827531 CNV gain 20364138
nsv954002 CNV duplication 24416366

Variation tolerance for CHRM3 Gene

Residual Variation Intolerance Score: 49% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.14; 38.86% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for CHRM3 Gene

Human Gene Mutation Database (HGMD)
SNPedia medical, phenotypic, and genealogical associations of SNPs for

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CHRM3 Gene

Disorders for CHRM3 Gene

MalaCards: The human disease database

(13) MalaCards diseases for CHRM3 Gene - From: HGMD, OMIM, ClinVar, GTR, Orphanet, DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
prune belly syndrome
  • pbs
cholinergic urticaria
bladder disease
  • urinary bladder disease
primary sjögren syndrome
  • primary sjögren-gougerot syndrome
aplasia of lacrimal and salivary glands
  • alsg
- elite association - COSMIC cancer census association via MalaCards
Search CHRM3 in MalaCards View complete list of genes associated with diseases


  • Prune belly syndrome (PBS) [MIM:100100]: A syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities. {ECO:0000269 PubMed:22077972}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Additional Disease Information for CHRM3

Genetic Association Database
Human Genome Epidemiology Navigator
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
genes like me logo Genes that share disorders with CHRM3: view

No data available for Genatlas for CHRM3 Gene

Publications for CHRM3 Gene

  1. Muscarinic M3 receptor stimulation increases cigarette smoke-induced IL-8 secretion by human airway smooth muscle cells. (PMID: 19460789) Gosens R … Zuyderduyn S (The European respiratory journal 2009) 3 22 25 58
  2. Sequence variation in the promoter region of the cholinergic receptor muscarinic 3 gene and asthma and atopy. (PMID: 12642833) Donfack J … Ober C (The Journal of allergy and clinical immunology 2003) 3 22 44 58
  3. The muscarinic M3 acetylcholine receptor exists as two differently sized complexes at the plasma membrane. (PMID: 23521066) Patowary S … Raicu V (The Biochemical journal 2013) 3 4 58
  4. Muscarinic Acetylcholine Receptor M3 Mutation Causes Urinary Bladder Disease and a Prune-Belly-like Syndrome. (PMID: 22077972) Weber S … Woolf AS (American journal of human genetics 2011) 3 4 58
  5. Family-based analysis of genetic variation underlying psychosis-inducing effects of cannabis: sibling analysis and proband follow-up. (PMID: 21041608) van Winkel R … Genetic Risk and Outcome of Psychosis (GROUP) Investigators (Archives of general psychiatry 2011) 3 44 58

Products for CHRM3 Gene

Sources for CHRM3 Gene

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