This gene product is a small calcium-binding protein that regulates cell pH by controlling plasma membrane-type Na+/H+ exchange activity. This protein shares sequence similarity with calcineurin B and can bind to and stimulate the protein phosphatase activity of calcineurin A (CnA) and functions in the calcineurin/NFAT (nuclear factor of activated T cells) signaling pathway. An... See more...

Aliases for CHP2 Gene

Aliases for CHP2 Gene

  • Calcineurin Like EF-Hand Protein 2 2 3 5
  • Hepatocellular Carcinoma-Associated Antigen 520 3 4
  • Calcineurin B Homologous Protein 2 3 4
  • Hepatocellular Carcinoma Antigen Gene 520 3
  • HCA520 4

External Ids for CHP2 Gene

Previous GeneCards Identifiers for CHP2 Gene

  • GC16P023674
  • GC16P023765
  • GC16P021855
  • GC16P023835
  • GC16P023846
  • GC16P023852
  • GC16P023766
  • GC16P023782
  • GC16P023800
  • GC16P023811
  • GC16P023824

Summaries for CHP2 Gene

Entrez Gene Summary for CHP2 Gene

  • This gene product is a small calcium-binding protein that regulates cell pH by controlling plasma membrane-type Na+/H+ exchange activity. This protein shares sequence similarity with calcineurin B and can bind to and stimulate the protein phosphatase activity of calcineurin A (CnA) and functions in the calcineurin/NFAT (nuclear factor of activated T cells) signaling pathway. Another member of the CHP subfamily, Calcineurin B homologous protein 1, is located on Chromosome 15 and is an inhibitor of calcineurin activity and has a genetic phenotype associated with Parkinson's Disease (OMIM:606988). This gene was initially identified as a tumor-associated antigen and was previously referred to as Hepatocellular carcinoma-associated antigen 520. [provided by RefSeq, Jul 2013]

GeneCards Summary for CHP2 Gene

CHP2 (Calcineurin Like EF-Hand Protein 2) is a Protein Coding gene. Diseases associated with CHP2 include Hepatocellular Carcinoma. Among its related pathways are Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins.. Gene Ontology (GO) annotations related to this gene include calcium ion binding. An important paralog of this gene is CHP1.

UniProtKB/Swiss-Prot Summary for CHP2 Gene

  • Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death. Also plays a role in the regulation of cell proliferation and tumor growth by increasing the phosphatase activity of PPP3CA in a calcium-dependent manner. Activator of the calcineurin/NFAT signaling pathway. Involved in the cytoplasmic translocation of the transcription factor NFATC3 to the nucleus.

Additional gene information for CHP2 Gene

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for CHP2 Gene

Genomics for CHP2 Gene

GeneHancer (GH) Regulatory Elements for CHP2 Gene

Promoters and enhancers for CHP2 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH16J023754 Promoter/Enhancer 1.5 EPDnew Ensembl ENCODE 500.7 +0.6 571 2.1 ZIC2 ZNF423 ZBTB26 KLF7 CTBP1 ZNF341 EZH2 KDM5B KLF9 NFIC CHP2 ENSG00000279756 piR-41306-100 PRKCB
GH16J023785 Enhancer 0.8 Ensembl ENCODE 28.7 +32.2 32208 1.9 USF1 TOE1 NANOG MITF USF2 SRF CHD7 EP300 RAD51 SP1 CHP2 PRKCB lnc-CHP2-2 piR-38579-017
GH16J023676 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE CraniofacialAtlas 10.3 -75.6 -75564 4.7 EP300 FOXK2 ZNF217 CTCF SIN3A MYC NRF1 USF1 POLR2G SP1 PLK1 piR-46201 DCTN5 PALB2 RBBP6 ENSG00000261723 ERN2 GGA2 ENSG00000260751 NDUFAB1
GH16J023835 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 7.4 +82.4 82374 3.6 HNRNPK EP300 CTCF SIN3A USF1 SP1 ZIC2 POLR2A ZBTB11 AFF1 PRKCB CHP2 lnc-CHP2-1
GH16J023807 Enhancer 0.9 FANTOM5 Ensembl ENCODE 17.3 +52.4 52374 2.8 SPI1 EBF1 IKZF1 PRDM10 MAX PAX5 ZFP36 IKZF2 RUNX3 SKIL CHP2 PRKCB GGA2 piR-38579-017 lnc-CHP2-2
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around CHP2 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for CHP2

Top Transcription factor binding sites by QIAGEN in the CHP2 gene promoter:
  • AhR
  • AREB6
  • c-Myb
  • Evi-1
  • GATA-3
  • POU3F2
  • PPAR-alpha
  • Sox9
  • Spz1
  • SRY

Genomic Locations for CHP2 Gene

Genomic Locations for CHP2 Gene
chr16:23,754,627-23,758,951
(GRCh38/hg38)
Size:
4,325 bases
Orientation:
Plus strand
chr16:23,765,948-23,770,272
(GRCh37/hg19)
Size:
4,325 bases
Orientation:
Plus strand

Genomic View for CHP2 Gene

Genes around CHP2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CHP2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CHP2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CHP2 Gene

Proteins for CHP2 Gene

  • Protein details for CHP2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O43745-CHP2_HUMAN
    Recommended name:
    Calcineurin B homologous protein 2
    Protein Accession:
    O43745
    Secondary Accessions:
    • A8K2I8

    Protein attributes for CHP2 Gene

    Size:
    196 amino acids
    Molecular mass:
    22452 Da
    Quaternary structure:
    • Interacts with PPP3CA. Interacts with SLC9A1/NHE1; the interaction occurs in a calcium-dependent manner.

    Three dimensional structures from OCA and Proteopedia for CHP2 Gene

neXtProt entry for CHP2 Gene

Post-translational modifications for CHP2 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for CHP2 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for CHP2 Gene

Domains & Families for CHP2 Gene

Gene Families for CHP2 Gene

HGNC:
Human Protein Atlas (HPA):
  • Cancer-related genes
  • Predicted intracellular proteins

Protein Domains for CHP2 Gene

Suggested Antigen Peptide Sequences for CHP2 Gene

GenScript: Design optimal peptide antigens:
  • cDNA, FLJ93539, Homo sapiens hepatocellular carcinoma antigen gene 520 (LOC63928),mRNA (A8K2I8_HUMAN)
  • Hepatocellular carcinoma-associated antigen 520 (CHP2_HUMAN)

Graphical View of Domain Structure for InterPro Entry

O43745

UniProtKB/Swiss-Prot:

CHP2_HUMAN :
  • Belongs to the calcineurin regulatory subunit family. CHP subfamily.
Family:
  • Belongs to the calcineurin regulatory subunit family. CHP subfamily.
genes like me logo Genes that share domains with CHP2: view

Function for CHP2 Gene

Molecular function for CHP2 Gene

UniProtKB/Swiss-Prot Function:
Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death. Also plays a role in the regulation of cell proliferation and tumor growth by increasing the phosphatase activity of PPP3CA in a calcium-dependent manner. Activator of the calcineurin/NFAT signaling pathway. Involved in the cytoplasmic translocation of the transcription factor NFATC3 to the nucleus.

Phenotypes From GWAS Catalog for CHP2 Gene

Gene Ontology (GO) - Molecular Function for CHP2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005509 calcium ion binding IDA 21392185
GO:0005515 protein binding IPI 21392185
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with CHP2: view
genes like me logo Genes that share phenotypes with CHP2: view

Animal Models for CHP2 Gene

MGI Knock Outs for CHP2:
  • Chp2 Chp2<tm1b(KOMP)Mbp>

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CHP2

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , miRNA , Transcription Factor Targets and HOMER Transcription for CHP2 Gene

Localization for CHP2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CHP2 Gene

Nucleus. Cytoplasm. Cell membrane. Note=Predominantly localized in a juxtanuclear region. Colocalizes with SLC9A3 in the juxtanuclear region and at the plasma membrane (By similarity). Exported from the nucleus to the cytoplasm through a nuclear export signal (NES) pathway. May shuttle between nucleus and cytoplasm. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CHP2 gene
Compartment Confidence
plasma membrane 5
nucleus 5
cytosol 2
extracellular 0

Gene Ontology (GO) - Cellular Components for CHP2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA,IEA 21392185
GO:0005737 cytoplasm IEA,IDA 21392185
GO:0005886 plasma membrane IEA,IDA 21392185
GO:0016020 membrane IEA --
genes like me logo Genes that share ontologies with CHP2: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for CHP2 Gene

Pathways & Interactions for CHP2 Gene

genes like me logo Genes that share pathways with CHP2: view

Pathways by source for CHP2 Gene

1 BioSystems pathway for CHP2 Gene

Gene Ontology (GO) - Biological Process for CHP2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0008284 positive regulation of cell proliferation IDA 18815128
GO:0010922 positive regulation of phosphatase activity IDA 18815128
GO:0015031 protein transport IEA --
GO:0042307 positive regulation of protein import into nucleus IDA 18815128
GO:0045944 positive regulation of transcription by RNA polymerase II IDA 18815128
genes like me logo Genes that share ontologies with CHP2: view

No data available for SIGNOR curated interactions for CHP2 Gene

Drugs & Compounds for CHP2 Gene

No Compound Related Data Available

Transcripts for CHP2 Gene

mRNA/cDNA for CHP2 Gene

1 REFSEQ mRNAs :
4 NCBI additional mRNA sequence :
1 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CHP2

Alternative Splicing Database (ASD) splice patterns (SP) for CHP2 Gene

No ASD Table

Relevant External Links for CHP2 Gene

GeneLoc Exon Structure for
CHP2

Expression for CHP2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for CHP2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for CHP2 Gene

This gene is overexpressed in Colon - Transverse (x15.2), Skin - Not Sun Exposed (Suprapubic) (x11.3), Skin - Sun Exposed (Lower leg) (x10.1), and Small Intestine - Terminal Ileum (x9.1).

Protein differential expression in normal tissues from HIPED for CHP2 Gene

This gene is overexpressed in Monocytes (34.2), Skin (9.7), Colon (7.4), and Rectum (6.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for CHP2 Gene



Transcriptomic regulation report from SPP (The Signaling Pathways Project) for CHP2

SOURCE GeneReport for Unigene cluster for CHP2 Gene:

Hs.178589

mRNA Expression by UniProt/SwissProt for CHP2 Gene:

O43745-CHP2_HUMAN
Tissue specificity: Expressed in malignantly transformed cells but not detected in normal tissues.

Evidence on tissue expression from TISSUES for CHP2 Gene

  • Intestine(4.3)
  • Nervous system(4.1)
genes like me logo Genes that share expression patterns with CHP2: view

No data available for Protein tissue co-expression partners and Phenotype-based relationships between genes and organs from Gene ORGANizer for CHP2 Gene

Orthologs for CHP2 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for CHP2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia CHP2 31 30
  • 99.83 (n)
OneToOne
dog
(Canis familiaris)
Mammalia CHP2 31
  • 86 (a)
OneToOne
cow
(Bos Taurus)
Mammalia CHP2 31 30
  • 85.03 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Chp2 17 31 30
  • 83.84 (n)
rat
(Rattus norvegicus)
Mammalia Chp2 30
  • 83.84 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia CHP2 31
  • 61 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia CHP2 31
  • 64 (a)
OneToOne
zebrafish
(Danio rerio)
Actinopterygii chp2 31 30
  • 64.95 (n)
OneToOne
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.7594 30
fruit fly
(Drosophila melanogaster)
Insecta elm 31
  • 49 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea pbo-1 31 30
  • 53.62 (n)
ManyToMany
ZK856.8 31
  • 46 (a)
ManyToMany
F59D6.7 31
  • 45 (a)
ManyToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes CNB1 31
  • 38 (a)
OneToMany
Species where no ortholog for CHP2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)

Evolution for CHP2 Gene

ENSEMBL:
Gene Tree for CHP2 (if available)
TreeFam:
Gene Tree for CHP2 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for CHP2: view image

Paralogs for CHP2 Gene

Paralogs for CHP2 Gene

(5) SIMAP similar genes for CHP2 Gene using alignment to 1 proteins:

  • CHP2_HUMAN
genes like me logo Genes that share paralogs with CHP2: view

Variants for CHP2 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for CHP2 Gene

SNP ID Clinical significance and condition Chr 16 pos Variation AA Info Type
rs35641939 - p.Arg127Pro

Additional dbSNP identifiers (rs#s) for CHP2 Gene

Structural Variations from Database of Genomic Variants (DGV) for CHP2 Gene

Variant ID Type Subtype PubMed ID
nsv1064223 CNV loss 25217958
nsv510681 CNV deletion 20534489

Variation tolerance for CHP2 Gene

Residual Variation Intolerance Score: 88.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.66; 56.95% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for CHP2 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
CHP2

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CHP2 Gene

Disorders for CHP2 Gene

MalaCards: The human disease database

(1) MalaCards diseases for CHP2 Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
hepatocellular carcinoma
  • hcc
- elite association - COSMIC cancer census association via MalaCards
Search CHP2 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for CHP2

genes like me logo Genes that share disorders with CHP2: view

No data available for UniProtKB/Swiss-Prot and Genatlas for CHP2 Gene

Publications for CHP2 Gene

  1. Expression of calcineurin B homologous protein 2 protects serum deprivation-induced cell death by serum-independent activation of Na+/H+ exchanger. (PMID: 12226101) Pang T … Shigekawa M (The Journal of biological chemistry 2002) 2 3 4 23 54
  2. Duffy-null-associated low neutrophil counts influence HIV-1 susceptibility in high-risk South African black women. (PMID: 21507922) Ramsuran V … Ndung'u T (Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 2011) 3 41 54
  3. Nuclear accumulation of calcineurin B homologous protein 2 (CHP2) results in enhanced proliferation of tumor cells. (PMID: 21392185) Li QH … Pang TX (Genes to cells : devoted to molecular & cellular mechanisms 2011) 3 4 54
  4. CHP2 activates the calcineurin/nuclear factor of activated T cells signaling pathway and enhances the oncogenic potential of HEK293 cells. (PMID: 18815128) Li GD … Zhang Y (The Journal of biological chemistry 2008) 3 4 54
  5. Overexpression of CHP2 enhances tumor cell growth, invasion and metastasis in ovarian cancer. (PMID: 17708351) Jin Q … Yang Q (In vivo (Athens, Greece) 2007) 3 23 54

Products for CHP2 Gene

Sources for CHP2 Gene