The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul... See more...

Aliases for CHKA Gene

Aliases for CHKA Gene

  • Choline Kinase Alpha 2 3 4 5
  • CKI 2 3 4
  • Ethanolamine Kinase 3 4
  • CHETK-Alpha 3 4
  • CHK 3 4
  • CK 3 4
  • EK 3 4
  • Choline Kinase 2
  • EC 2.7.1.32 4
  • EC 2.7.1.82 4
  • CHKA 5

External Ids for CHKA Gene

Previous HGNC Symbols for CHKA Gene

  • CHK

Previous GeneCards Identifiers for CHKA Gene

  • GC11M067596
  • GC11M067576
  • GC11M067820
  • GC11M064155

Summaries for CHKA Gene

Entrez Gene Summary for CHKA Gene

  • The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

GeneCards Summary for CHKA Gene

CHKA (Choline Kinase Alpha) is a Protein Coding gene. Diseases associated with CHKA include West Nile Encephalitis and Hepatitis C Virus. Among its related pathways are Glycerophospholipid biosynthesis and Metabolism. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and obsolete signal transducer activity. An important paralog of this gene is CHKB.

UniProtKB/Swiss-Prot Summary for CHKA Gene

  • Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline.

Gene Wiki entry for CHKA Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for CHKA Gene

Genomics for CHKA Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for CHKA Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around CHKA on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for CHKA

Top Transcription factor binding sites by QIAGEN in the CHKA gene promoter:
  • AP-1
  • ATF-2
  • c-Jun
  • NRSF form 1
  • NRSF form 2

Genomic Locations for CHKA Gene

Latest Assembly
chr11:68,052,859-68,121,444
(GRCh38/hg38)
Size:
68,586 bases
Orientation:
Minus strand

Previous Assembly
chr11:67,820,326-67,888,855
(GRCh37/hg19 by Entrez Gene)
Size:
68,530 bases
Orientation:
Minus strand

chr11:67,820,326-67,888,911
(GRCh37/hg19 by Ensembl)
Size:
68,586 bases
Orientation:
Minus strand

Genomic View for CHKA Gene

Genes around CHKA on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CHKA Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CHKA Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CHKA Gene

Proteins for CHKA Gene

  • Protein details for CHKA Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P35790-CHKA_HUMAN
    Recommended name:
    Choline kinase alpha
    Protein Accession:
    P35790
    Secondary Accessions:
    • Q8NE29

    Protein attributes for CHKA Gene

    Size:
    457 amino acids
    Molecular mass:
    52249 Da
    Quaternary structure:
    • Heterodimer with CHKB (By similarity). Homodimer.
    • (Microbial infection) Interacts with PI4KA/PI4KIIIalpha; CHKA bridges PI4KA/PI4KIIIalpha and hepatitis C virus (HCV) non-structural protein 5A (NS5A) and potentiates NS5A-stimulated PI4KA activity, which then facilitates the targeting of the ternary complex to the ER for viral replication.

    Three dimensional structures from OCA and Proteopedia for CHKA Gene

    Alternative splice isoforms for CHKA Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for CHKA Gene

Post-translational modifications for CHKA Gene

  • Ubiquitination at Lys195
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for CHKA Gene

Domains & Families for CHKA Gene

Gene Families for CHKA Gene

Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins

Protein Domains for CHKA Gene

InterPro:
Blocks:
  • Choline/ethanolamine kinase

Suggested Antigen Peptide Sequences for CHKA Gene

GenScript: Design optimal peptide antigens:
  • Ethanolamine kinase (CHKA_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P35790

UniProtKB/Swiss-Prot:

CHKA_HUMAN :
  • Belongs to the choline/ethanolamine kinase family.
Family:
  • Belongs to the choline/ethanolamine kinase family.
genes like me logo Genes that share domains with CHKA: view

Function for CHKA Gene

Molecular function for CHKA Gene

UniProtKB/Swiss-Prot Function:
Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + choline = ADP + H(+) + phosphocholine; Xref=Rhea:RHEA:12837, ChEBI:CHEBI:15354, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:295975, ChEBI:CHEBI:456216; EC=2.7.1.32; Evidence={ECO:0000269|PubMed:19915674};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + ethanolamine = ADP + H(+) + phosphoethanolamine; Xref=Rhea:RHEA:13069, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:57603, ChEBI:CHEBI:58190, ChEBI:CHEBI:456216; EC=2.7.1.82; Evidence={ECO:0000269|PubMed:19915674};.
UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=0.2 mM for choline {ECO:0000269|PubMed:17007874, ECO:0000269|PubMed:19915674}; KM=0.4 mM for ATP {ECO:0000269|PubMed:17007874, ECO:0000269|PubMed:19915674}; KM=12 mM for ethanolamine {ECO:0000269|PubMed:17007874, ECO:0000269|PubMed:19915674};

Enzyme Numbers (IUBMB) for CHKA Gene

Phenotypes From GWAS Catalog for CHKA Gene

Gene Ontology (GO) - Molecular Function for CHKA Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0004103 choline kinase activity TAS,IDA 19915674
GO:0004104 cholinesterase activity IEA --
GO:0004305 ethanolamine kinase activity IEA,IDA 19915674
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with CHKA: view
genes like me logo Genes that share phenotypes with CHKA: view

Animal Models for CHKA Gene

MGI Knock Outs for CHKA:
  • Chka Chka<tm2a(KOMP)Wtsi>

Animal Models for research

  • Taconic Biosciences Mouse Models for CHKA

miRNA for CHKA Gene

miRTarBase miRNAs that target CHKA

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CHKA

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for CHKA Gene

Localization for CHKA Gene

Subcellular locations from UniProtKB/Swiss-Prot for CHKA Gene

Cytoplasm.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CHKA gene
Compartment Confidence
cytosol 5
plasma membrane 2
extracellular 2
cytoskeleton 2
mitochondrion 2
nucleus 2
endoplasmic reticulum 2
peroxisome 1
endosome 1
lysosome 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Endoplasmic reticulum (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for CHKA Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IEA --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with CHKA: view

Pathways & Interactions for CHKA Gene

genes like me logo Genes that share pathways with CHKA: view

Pathways by source for CHKA Gene

UniProtKB/Swiss-Prot P35790-CHKA_HUMAN

  • Pathway: Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphocholine from choline: step 1/1.
  • Pathway: Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.

Gene Ontology (GO) - Biological Process for CHKA Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006580 ethanolamine metabolic process IEA --
GO:0006629 lipid metabolic process TAS 10363580
GO:0006646 phosphatidylethanolamine biosynthetic process TAS --
GO:0006656 phosphatidylcholine biosynthetic process TAS --
GO:0006657 CDP-choline pathway IEA --
genes like me logo Genes that share ontologies with CHKA: view

No data available for SIGNOR curated interactions for CHKA Gene

Drugs & Compounds for CHKA Gene

(52) Drugs for CHKA Gene - From: DrugBank, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Choline Approved Nutra Enzyme, substrate 240
Choline salicylate Approved Nutra Enzyme, substrate 0
Ethanolamine Experimental Pharma 0
L-Dilinoleoyllecithin Experimental Pharma 0
Phosphorylcolamine Experimental Pharma 0

(38) Additional Compounds for CHKA Gene - From: Novoseek and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • 5'-Adenylphosphoric acid
  • Adenosine 5'-diphosphate
  • ADENOSINE-5'-diphosphATE
  • H3ADP
  • 5'-Adenylphosphate
58-64-0
phosphorylcholine
  • Choline phosphate
  • CHOP
  • N-Trimethyl-2-aminoethylphosphonate
  • O-Phosphocholine
  • Phosphoryl-choline
3616-04-4
CE(22:0)
  • 1-Tetradecanoyl-2-octadecanoyl-sn-glycero-3-phosphocholine
  • GPCho(14:0/18:0)
  • Phosphatidylcholine(14:0/18:0)
  • 1-Myristoyl-2-stearoylphosphatidylcholine
  • 1m-2S-PC
61510-09-6
PC(18:1(9Z)e/2:0)
  • (2-Acetyloxy-3-octadec-9-enoxypropyl) 2-trimethylazaniumylethyl phosphate
  • 2-Acetyl-1-(9Z-octadecenyl)-sn-glycero-3-phosphocholine
  • PC(O-18:1(9Z)/2:0)
PC(20:2(11Z,14Z)/18:1(11Z))
  • Phosphatidylcholine(20:2/18:1)
  • GPCho(20:2/18:1)
  • Phosphatidylcholine(38:3)
  • PC(38:3)
  • Lecithin
genes like me logo Genes that share compounds with CHKA: view

Transcripts for CHKA Gene

mRNA/cDNA for CHKA Gene

6 REFSEQ mRNAs :
11 NCBI additional mRNA sequence :
8 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CHKA

Alternative Splicing Database (ASD) splice patterns (SP) for CHKA Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13a · 13b · 13c · 13d ^ 14a · 14b · 14c · 14d
SP1: - - - - -
SP2: - - - - - -
SP3:
SP4:
SP5: -
SP6:

Relevant External Links for CHKA Gene

GeneLoc Exon Structure for
CHKA

Expression for CHKA Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for CHKA Gene

Protein differential expression in normal tissues from HIPED for CHKA Gene

This gene is overexpressed in Lung (55.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for CHKA Gene



Protein tissue co-expression partners for CHKA Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for CHKA

SOURCE GeneReport for Unigene cluster for CHKA Gene:

Hs.77221

Evidence on tissue expression from TISSUES for CHKA Gene

  • Nervous system(3.1)
  • Kidney(2.5)
  • Liver(2.5)
  • Intestine(2.4)
  • Bone marrow(2.2)
  • Stomach(2.2)
  • Skin(2.2)
  • Pancreas(2.1)
  • Thyroid gland(2.1)
  • Muscle(2.1)
  • Lung(2.1)
  • Spleen(2.1)
  • Heart(2.1)
  • Lymph node(2)
genes like me logo Genes that share expression patterns with CHKA: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for CHKA Gene

Orthologs for CHKA Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for CHKA Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia CHKA 29 30
  • 99.74 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia CHKA 29 30
  • 88.6 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia CHKA 29 30
  • 88.07 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Chka 29
  • 86.44 (n)
Mouse
(Mus musculus)
Mammalia Chka 29 16 30
  • 86.39 (n)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia CHKA 30
  • 76 (a)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia CHKA 30
  • 74 (a)
OneToOne
Chicken
(Gallus gallus)
Aves CHKA 29 30
  • 75.88 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia CHKA 30
  • 78 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia chka 29
  • 68.64 (n)
African clawed frog
(Xenopus laevis)
Amphibia Xl.12834 29
Zebrafish
(Danio rerio)
Actinopterygii chka 29 30
  • 70.11 (n)
OneToOne
Fruit Fly
(Drosophila melanogaster)
Insecta CG2201 30
  • 26 (a)
OneToMany
Worm
(Caenorhabditis elegans)
Secernentea cka-2 29 30
  • 51.96 (n)
ManyToMany
cka-1 30
  • 30 (a)
ManyToMany
ckb-1 30
  • 27 (a)
ManyToMany
ckb-4 30
  • 25 (a)
ManyToMany
ckb-3 30
  • 24 (a)
ManyToMany
ckb-2 30
  • 24 (a)
ManyToMany
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes EKI1 30 32
  • 17 (a)
ManyToMany
CKI1 30
  • 17 (a)
ManyToMany
Thale Cress
(Arabidopsis thaliana)
eudicotyledons CK1 29
  • 46.39 (n)
Rice
(Oryza sativa)
Liliopsida Os01g0183000 29
  • 48.49 (n)
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 30
  • 40 (a)
OneToMany
Species where no ortholog for CHKA was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for CHKA Gene

ENSEMBL:
Gene Tree for CHKA (if available)
TreeFam:
Gene Tree for CHKA (if available)
Aminode:
Evolutionary constrained regions (ECRs) for CHKA: view image
Alliance of Genome Resources:
Additional Orthologs for CHKA

Paralogs for CHKA Gene

Paralogs for CHKA Gene

(2) SIMAP similar genes for CHKA Gene using alignment to 3 proteins:

  • CHKA_HUMAN
  • E9PM06_HUMAN
  • H0YD02_HUMAN
genes like me logo Genes that share paralogs with CHKA: view

Variants for CHKA Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for CHKA Gene

SNP ID Clinical significance and condition Chr 11 pos Variation AA Info Type
rs17853641 -- p.Ser220Gly
rs17853642 -- p.Leu422Gln

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for CHKA Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for CHKA Gene

Variant ID Type Subtype PubMed ID
dgv1221n100 CNV gain 25217958
dgv1983n54 CNV loss 21841781
esv2677355 CNV deletion 23128226
esv2759835 CNV gain+loss 17122850
esv3626732 CNV loss 21293372
nsv1070047 CNV deletion 25765185
nsv1143691 CNV deletion 24896259
nsv371 CNV deletion 18451855
nsv469856 CNV gain 16826518
nsv469964 CNV loss 18288195
nsv825963 CNV gain 20364138
nsv825965 CNV gain 20364138
nsv951026 CNV deletion 24416366

Variation tolerance for CHKA Gene

Residual Variation Intolerance Score: 28.3% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.09; 22.15% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for CHKA Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
CHKA
Leiden Open Variation Database (LOVD)
CHKA

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CHKA Gene

Disorders for CHKA Gene

MalaCards: The human disease database

(18) MalaCards diseases for CHKA Gene - From: COP and GCD

Disorder Aliases PubMed IDs
west nile encephalitis
  • west nile fever encephalitis
hepatitis c virus
  • hepatitis c virus, susceptibility to
cecal benign neoplasm
  • cecum neoplasm
cecum adenoma
  • caecum adenoma
neuroleptic malignant syndrome
- elite association - COSMIC cancer census association via MalaCards
Search CHKA in MalaCards View complete list of genes associated with diseases

Additional Disease Information for CHKA

Human Genome Epidemiology Navigator
(HuGE)
Atlas
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
Open Targets Platform
genes like me logo Genes that share disorders with CHKA: view

No data available for UniProtKB/Swiss-Prot and Genatlas for CHKA Gene

Publications for CHKA Gene

  1. Differential role of human choline kinase alpha and beta enzymes in lipid metabolism: implications in cancer onset and treatment. (PMID: 19915674) Gallego-Ortega D … Lacal JC (PloS one 2009) 3 4 22
  2. Structure and function of choline kinase isoforms in mammalian cells. (PMID: 15003397) Aoyama C … Ishidate K (Progress in lipid research 2004) 2 3 22
  3. Cloning of a human choline kinase cDNA by complementation of the yeast cki mutation. (PMID: 1618328) Hosaka K … Yamashita S (FEBS letters 1992) 2 3 4
  4. Hepatitis C Virus Subverts Human Choline Kinase-α To Bridge Phosphatidylinositol-4-Kinase IIIα (PI4KIIIα) and NS5A and Upregulates PI4KIIIα Activation, Thereby Promoting the Translocation of the Ternary Complex to the Endoplasmic Reticulum for Viral Replication. (PMID: 28566381) Wong MT … Chen SS (Journal of virology 2017) 3 4
  5. Crystal structures of human choline kinase isoforms in complex with hemicholinium-3: single amino acid near the active site influences inhibitor sensitivity. (PMID: 20299452) Hong BS … Park HW (The Journal of biological chemistry 2010) 3 4

Products for CHKA Gene

Sources for CHKA Gene