The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphor... See more...

Aliases for CHEK1 Gene

Aliases for CHEK1 Gene

  • Checkpoint Kinase 1 2 3 5
  • CHK1 2 3 4
  • Serine/Threonine-Protein Kinase Chk1 3 4
  • Cell Cycle Checkpoint Kinase 3 4
  • CHK1 Checkpoint Homolog 3 4
  • EC 2.7.11.1 4 50
  • Checkpoint, S. Pombe, Homolog Of, 1 3
  • CHK1 (Checkpoint, S.Pombe) Homolog 2
  • CHK1 Checkpoint Homolog (S. Pombe) 2
  • Checkpoint Kinase-1 4
  • EC 2.7.11 50
  • Chk1-S 3
  • CHEK1 5

External Ids for CHEK1 Gene

Previous GeneCards Identifiers for CHEK1 Gene

  • GC11P127497
  • GC11P127009
  • GC11P125529
  • GC11P125033
  • GC11P125001
  • GC11P125495
  • GC11P121436

Summaries for CHEK1 Gene

Entrez Gene Summary for CHEK1 Gene

  • The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

CIViC Summary for CHEK1 Gene

GeneCards Summary for CHEK1 Gene

CHEK1 (Checkpoint Kinase 1) is a Protein Coding gene. Diseases associated with CHEK1 include Ataxia-Telangiectasia and Li-Fraumeni Syndrome. Among its related pathways are RET signaling and DNA Double-Strand Break Repair. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity.

UniProtKB/Swiss-Prot Summary for CHEK1 Gene

  • Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA (PubMed:11535615, PubMed:12446774, PubMed:12399544, PubMed:14559997, PubMed:14988723, PubMed:15311285, PubMed:15665856, PubMed:15650047). May also negatively regulate cell cycle progression during unperturbed cell cycles (PubMed:11535615, PubMed:12446774, PubMed:12399544, PubMed:14559997, PubMed:14988723, PubMed:15311285, PubMed:15665856, PubMed:15650047). This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome (PubMed:11535615, PubMed:12446774, PubMed:12399544, PubMed:14559997, PubMed:14988723, PubMed:15311285, PubMed:15665856, PubMed:15650047). Recognizes the substrate consensus sequence [R-X-X-S/T] (PubMed:11535615, PubMed:12446774, PubMed:12399544, PubMed:14559997, PubMed:14988723, PubMed:15311285, PubMed:15665856, PubMed:15650047). Binds to and phosphorylates CDC25A, CDC25B and CDC25C (PubMed:9278511, PubMed:12676583, PubMed:14681206, PubMed:12676925, PubMed:12759351, PubMed:19734889, PubMed:14559997). Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C (PubMed:9278511). Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A (PubMed:9278511, PubMed:12676583, PubMed:14681206, PubMed:12676925, PubMed:12759351, PubMed:19734889). Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A (PubMed:9278511, PubMed:19734889, PubMed:20090422). Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression (PubMed:9278511). Also phosphorylates NEK6 (PubMed:18728393). Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination (PubMed:15665856). Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation (PubMed:10673501, PubMed:15659650, PubMed:16511572). Also promotes repair of DNA cross-links through phosphorylation of FANCE (PubMed:17296736). Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A (PubMed:12660173, PubMed:12955071). This may enhance chromatin assembly both in the presence or absence of DNA damage (PubMed:12660173, PubMed:12955071). May also play a role in replication fork maintenance through regulation of PCNA (PubMed:18451105). May regulate the transcription of genes that regulate cell-cycle progression through the phosphorylation of histones (By similarity). Phosphorylates histone H3.1 (to form H3T11ph), which leads to epigenetic inhibition of a subset of genes (By similarity). May also phosphorylate RB1 to promote its interaction with the E2F family of transcription factors and subsequent cell cycle arrest (PubMed:17380128). Phosphorylates SPRTN, promoting SPRTN recruitment to chromatin (PubMed:31316063). Reduces replication stress and activates the G2/M checkpoint, by phosphorylating and inactivating PABIR1/FAM122A and promoting the serine/threonine-protein phosphatase 2A-mediated dephosphorylation and stabilization of WEE1 levels and activity (PubMed:33108758).
  • [Isoform 2]: Endogenous repressor of isoform 1, interacts with, and antagonizes CHK1 to promote the S to G2/M phase transition.

Tocris Summary for CHEK1 Gene

  • Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. Two subtypes have so far been identified, Chk1 and Chk2. They are essential components to delay cell cycle progression in all cells and act at all three cell cycle checkpoints.

Gene Wiki entry for CHEK1 Gene

No data available for PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for CHEK1 Gene

Genomics for CHEK1 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for CHEK1 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around CHEK1 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for CHEK1

Top Transcription factor binding sites by QIAGEN in the CHEK1 gene promoter:
  • AP-1
  • ATF-2
  • c-Jun
  • NF-AT1
  • p53

Genomic Locations for CHEK1 Gene

Latest Assembly
chr11:125,624,910-125,676,255
(GRCh38/hg38)
Size:
51,346 bases
Orientation:
Plus strand

Previous Assembly
chr11:125,495,031-125,546,150
(GRCh37/hg19 by Entrez Gene)
Size:
51,120 bases
Orientation:
Plus strand

chr11:125,495,036-125,546,150
(GRCh37/hg19 by Ensembl)
Size:
51,115 bases
Orientation:
Plus strand

Genomic View for CHEK1 Gene

Genes around CHEK1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CHEK1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CHEK1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CHEK1 Gene

Proteins for CHEK1 Gene

  • Protein details for CHEK1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O14757-CHK1_HUMAN
    Recommended name:
    Serine/threonine-protein kinase Chk1
    Protein Accession:
    O14757
    Secondary Accessions:
    • A8K934
    • B4DDD0
    • B4DSK3
    • B5BTY6
    • F5H7S4
    • H2BI51

    Protein attributes for CHEK1 Gene

    Size:
    476 amino acids
    Molecular mass:
    54434 Da
    Quaternary structure:
    • Interacts (phosphorylated by ATR) with RAD51 (PubMed:15665856). Interacts with and phosphorylates CLSPN, an adapter protein that regulates the ATR-dependent phosphorylation of CHEK1 (PubMed:16963448). Interacts with BRCA1 (PubMed:11836499). Interacts with and phosphorylates CDC25A, CDC25B and CDC25C (PubMed:9278511). Interacts with FBXO6, which regulates CHEK1 (PubMed:19716789). Interacts with PPM1D, which regulates CHEK1 through dephosphorylation (PubMed:15870257). Interacts with TIMELESS; DNA damage-dependent (PubMed:15798197). Interacts with FEM1B; activates CHEK1 in response to stress (PubMed:19330022). Interacts with TLK1 (PubMed:12660173). Interacts with XPO1 and YWHAZ (PubMed:12676962). Interacts with CDK5RAP3; antagonizes CHEK1 (PubMed:19223857).
    • [Isoform 1]: Isoform 1 associates with isoform 2, the interaction is disrupted upon phosphorylation by ATR.

    Three dimensional structures from OCA and Proteopedia for CHEK1 Gene

    Alternative splice isoforms for CHEK1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for CHEK1 Gene

Selected DME Specific Peptides for CHEK1 Gene

O14757:
  • VLTAMLAGELPWDQPSDSCQEYSDWKE
  • DFRLSKGDGLEFKRHFLKIK
  • PVDVWSCGIVLTAMLAGELPWDQP
  • HENVVKFYGHRREG
  • GGELFDR
  • RITIPDIKKDRW
  • YVAPELL
  • TEEAVAVKIVDM
  • DRRNNKLIFK
  • KPENLLLDE
  • DCPENIKKEICIN
  • WQRLVKRMTRFFTK
  • CGTLPYVAPE
  • HRDIKPEN
  • KIDSAPLALLHKIL

Post-translational modifications for CHEK1 Gene

  • Phosphorylated by ATR in a RAD17-dependent manner in response to ultraviolet irradiation and inhibition of DNA replication. Phosphorylated by ATM in response to ionizing irradiation. ATM and ATR can both phosphorylate Ser-317 and Ser-345 and this results in enhanced kinase activity. Phosphorylation at Ser-345 induces a change in the conformation of the protein, activates the kinase activity and is a prerequisite for interaction with FBXO6 and subsequent ubiquitination at Lys-436. Phosphorylation at Ser-345 also increases binding to 14-3-3 proteins and promotes nuclear retention. Conversely, dephosphorylation at Ser-345 by PPM1D may contribute to exit from checkpoint mediated cell cycle arrest. Phosphorylation at Ser-280 by AKT1/PKB, may promote mono and/or diubiquitination. Also phosphorylated at undefined residues during mitotic arrest, resulting in decreased activity.
  • Ubiquitinated. Mono or diubiquitination promotes nuclear exclusion (By similarity). The activated form (phosphorylated on Ser-345) is polyubiquitinated at Lys-436 by some SCF-type E3 ubiquitin ligase complex containing FBXO6 promoting its degradation. Ubiquitination and degradation are required to terminate the checkpoint and ensure that activated CHEK1 does not accumulate as cells progress through S phase, when replication forks encounter transient impediments during normal DNA replication.
  • Proteolytically cleaved at the C-terminus by SPRTN during normal DNA replication, thereby promoting CHEK1 removal from chromatin and activating the protein kinase activity.
  • Ubiquitination at Lys180, Lys244, Lys313, and Lys436
  • Modification sites at PhosphoSitePlus

Antibodies for research

  • Abcam antibodies for CHEK1

Domains & Families for CHEK1 Gene

Gene Families for CHEK1 Gene

IUPHAR :
Human Protein Atlas (HPA):
  • Cancer-related genes
  • Enzymes
  • Plasma proteins
  • Predicted intracellular proteins

Suggested Antigen Peptide Sequences for CHEK1 Gene

GenScript: Design optimal peptide antigens:
  • CHK1 checkpoint homolog (B5BTY6_HUMAN)
  • Serine/threonine-protein kinase Chk1 (CHK1_HUMAN)
  • CHEK1 (Q6W8P8_HUMAN)

Graphical View of Domain Structure for InterPro Entry

O14757

UniProtKB/Swiss-Prot:

CHK1_HUMAN :
  • The autoinhibitory region (AIR) inhibits the activity of the kinase domain.
  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.
Domain:
  • The autoinhibitory region (AIR) inhibits the activity of the kinase domain.
Family:
  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.
genes like me logo Genes that share domains with CHEK1: view

Function for CHEK1 Gene

Molecular function for CHEK1 Gene

UniProtKB/Swiss-Prot Function:
Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA (PubMed:11535615, PubMed:12446774, PubMed:12399544, PubMed:14559997, PubMed:14988723, PubMed:15311285, PubMed:15665856, PubMed:15650047). May also negatively regulate cell cycle progression during unperturbed cell cycles (PubMed:11535615, PubMed:12446774, PubMed:12399544, PubMed:14559997, PubMed:14988723, PubMed:15311285, PubMed:15665856, PubMed:15650047). This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome (PubMed:11535615, PubMed:12446774, PubMed:12399544, PubMed:14559997, PubMed:14988723, PubMed:15311285, PubMed:15665856, PubMed:15650047). Recognizes the substrate consensus sequence [R-X-X-S/T] (PubMed:11535615, PubMed:12446774, PubMed:12399544, PubMed:14559997, PubMed:14988723, PubMed:15311285, PubMed:15665856, PubMed:15650047). Binds to and phosphorylates CDC25A, CDC25B and CDC25C (PubMed:9278511, PubMed:12676583, PubMed:14681206, PubMed:12676925, PubMed:12759351, PubMed:19734889, PubMed:14559997). Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C (PubMed:9278511). Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A (PubMed:9278511, PubMed:12676583, PubMed:14681206, PubMed:12676925, PubMed:12759351, PubMed:19734889). Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A (PubMed:9278511, PubMed:19734889, PubMed:20090422). Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression (PubMed:9278511). Also phosphorylates NEK6 (PubMed:18728393). Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination (PubMed:15665856). Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation (PubMed:10673501, PubMed:15659650, PubMed:16511572). Also promotes repair of DNA cross-links through phosphorylation of FANCE (PubMed:17296736). Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A (PubMed:12660173, PubMed:12955071). This may enhance chromatin assembly both in the presence or absence of DNA damage (PubMed:12660173, PubMed:12955071). May also play a role in replication fork maintenance through regulation of PCNA (PubMed:18451105). May regulate the transcription of genes that regulate cell-cycle progression through the phosphorylation of histones (By similarity). Phosphorylates histone H3.1 (to form H3T11ph), which leads to epigenetic inhibition of a subset of genes (By similarity). May also phosphorylate RB1 to promote its interaction with the E2F family of transcription factors and subsequent cell cycle arrest (PubMed:17380128). Phosphorylates SPRTN, promoting SPRTN recruitment to chromatin (PubMed:31316063). Reduces replication stress and activates the G2/M checkpoint, by phosphorylating and inactivating PABIR1/FAM122A and promoting the serine/threonine-protein phosphatase 2A-mediated dephosphorylation and stabilization of WEE1 levels and activity (PubMed:33108758).
UniProtKB/Swiss-Prot Function:
[Isoform 2]: Endogenous repressor of isoform 1, interacts with, and antagonizes CHK1 to promote the S to G2/M phase transition.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1; Evidence={ECO:0000269|PubMed:10673501, ECO:0000269|PubMed:12660173, ECO:0000269|PubMed:14559997, ECO:0000269|PubMed:15659650, ECO:0000269|PubMed:15665856, ECO:0000269|PubMed:16511572, ECO:0000269|PubMed:16963448, ECO:0000269|PubMed:17296736, ECO:0000269|PubMed:17380128, ECO:0000269|PubMed:18317453, ECO:0000269|PubMed:18728393, ECO:0000269|PubMed:31316063, ECO:0000269|PubMed:9278511};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1; Evidence={ECO:0000269|PubMed:10673501, ECO:0000269|PubMed:12660173, ECO:0000269|PubMed:14559997, ECO:0000269|PubMed:15659650, ECO:0000269|PubMed:15665856, ECO:0000269|PubMed:16511572, ECO:0000269|PubMed:16963448, ECO:0000269|PubMed:17296736, ECO:0000269|PubMed:17380128, ECO:0000269|PubMed:18317453, ECO:0000269|PubMed:18728393, ECO:0000269|PubMed:31316063, ECO:0000269|PubMed:9278511};.
UniProtKB/Swiss-Prot EnzymeRegulation:
Activated through phosphorylation predominantly by ATR but also by ATM in response to DNA damage or inhibition of DNA replication (PubMed:11390642, PubMed:12588868, PubMed:12676583, PubMed:12676962, PubMed:15665856, PubMed:19716789). Activation is modulated by several mediators including CLSPN, BRCA1 and FEM1B (PubMed:11836499, PubMed:12766152, PubMed:16963448, PubMed:19330022). Proteolytic cleavage at the C-terminus by SPRTN during normal DNA replication activates the protein kinase activity (PubMed:31316063).
GENATLAS Biochemistry:
checkpoint,S pombe homolog 1,component of the G2/M checkpoint,phosphorylating and inactivating CDC25C,required for cell cycle arrest in reponse to DNA damage,in association with 14.3.3 proteins (YWHA*),altered in colon and endometrial tumors

Enzyme Numbers (IUBMB) for CHEK1 Gene

Phenotypes From GWAS Catalog for CHEK1 Gene

Gene Ontology (GO) - Molecular Function for CHEK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0004672 protein kinase activity IEA,IMP 22024163
GO:0004674 protein serine/threonine kinase activity TAS --
GO:0005515 protein binding IPI 11836499
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with CHEK1: view

Phenotypes for CHEK1 Gene

MGI mutant phenotypes for CHEK1:
inferred from 4 alleles
GenomeRNAi human phenotypes for CHEK1:
genes like me logo Genes that share phenotypes with CHEK1: view

Animal Models for CHEK1 Gene

MGI Knock Outs for CHEK1:

Animal Models for research

miRNA for CHEK1 Gene

miRTarBase miRNAs that target CHEK1

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CHEK1

Clone products for research

  • Addgene plasmids for CHEK1

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for CHEK1 Gene

Localization for CHEK1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CHEK1 Gene

Nucleus. Chromosome. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Nuclear export is mediated at least in part by XPO1/CRM1 (PubMed:12676962). Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B (PubMed:15311285). Proteolytic cleavage at the C-terminus by SPRTN promotes removal from chromatin (PubMed:31316063). {ECO:0000269 PubMed:12676962, ECO:0000269 PubMed:15311285, ECO:0000269 PubMed:31316063}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CHEK1 gene
Compartment Confidence
cytoskeleton 5
nucleus 5
cytosol 5
extracellular 4
plasma membrane 2
mitochondrion 2
endoplasmic reticulum 2
lysosome 2
peroxisome 1
endosome 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nucleoplasm (3)
  • Vesicles (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for CHEK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000781 colocalizes_with chromosome, telomeric region IDA 15149599
GO:0000785 chromatin ISS --
GO:0000794 condensed nuclear chromosome IDA 9382850
GO:0005615 extracellular space HDA 23580065
GO:0005634 nucleus IBA,IDA 15311285
genes like me logo Genes that share ontologies with CHEK1: view

Pathways & Interactions for CHEK1 Gene

genes like me logo Genes that share pathways with CHEK1: view

Pathways by source for CHEK1 Gene

4 GeneGo (Thomson Reuters) pathways for CHEK1 Gene
  • Cell cycle Role of 14-3-3 proteins in cell cycle regulation
  • Cell cycle Role of SCF complex in cell cycle regulation
  • DNA damage_ATM/ATR regulation of G1/S checkpoint
  • DNA damage_DNA-damage-induced responses
1 Tocris pathway for CHEK1 Gene
10 Qiagen pathways for CHEK1 Gene
  • 14-3-3 and Cell Cycle Regulation
  • Akt Signaling
  • ATM Pathway
  • BRCA1 Pathway
  • Chks in Checkpoint Regulation
1 Cell Signaling Technology pathway for CHEK1 Gene
3 GeneTex pathways for CHEK1 Gene

SIGNOR curated interactions for CHEK1 Gene

Gene Ontology (GO) - Biological Process for CHEK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000077 DNA damage checkpoint IMP 19716789
GO:0000086 G2/M transition of mitotic cell cycle IEA --
GO:0001833 inner cell mass cell proliferation IEA --
GO:0006260 DNA replication TAS --
GO:0006281 DNA repair IMP 19716789
genes like me logo Genes that share ontologies with CHEK1: view

Drugs & Compounds for CHEK1 Gene

(83) Drugs for CHEK1 Gene - From: DrugBank, ApexBio, DGIdb, HMDB, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Lacosamide Approved Pharma inhibitor Checkpoint kinase inhibitor,ATP competitive, Checkpoint kinase (Chk) inhibitor 95
fostamatinib Approved, Investigational Pharma Target, inhibitor Kinase Inhibitors 0
Floxuridine Approved Pharma Antineoplastic antimetabolite, Inhibitor of thymidylate synthetase; anticancer agent, RNA and DNA synthesis inhibitor 59
CHIR-124 Experimental Pharma Target Chk1 inhibitor,novel and potent 0
(2R)-1-[(5,6-DIPHENYL-7H-PYRROLO[2,3-D]PYRIMIDIN-4-YL)AMINO]PROPAN-2-OL Experimental Pharma Target 0

(24) Additional Compounds for CHEK1 Gene - From: Novoseek, HMDB, and Tocris

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • 5'-Adenylphosphoric acid
  • Adenosine 5'-diphosphate
  • ADENOSINE-5'-diphosphATE
  • H3ADP
  • 5'-Adenylphosphate
58-64-0
DIM
1968-05-4
PD 407824
622864-54-4

(5) Tocris Compounds for CHEK1 Gene

Compound Action Cas Number
DIM Activates Chk2; induces G2/M cell cycle arrest 1968-05-4
NSC 109555 ditosylate Selective Chk2 inhibitor 66748-43-4
PD 407824 Selective inhibitor of Chk1 and Wee1 622864-54-4
SB 218078 Inhibitor of checkpoint kinase 1 (Chk1) 135897-06-2
TCS 2312 Potent Chk1 inhibitor 838823-31-7

(12) ApexBio Compounds for CHEK1 Gene

Compound Action Cas Number
AZD7762 Checkpoint kinase inhibitor,ATP competitive 860352-01-8
CCT244747 Potent and selective CHK1 inhibitor 1404095-34-6
CHIR-124 Chk1 inhibitor,novel and potent 405168-58-3
Chk1 and MK2 Inhibitors set Chk1- and MK2 inhibitors
Floxuridine Antineoplastic antimetabolite 50-91-9
LY2603618 Chk1 inhibitor,highly selective 911222-45-2
LY2606368 CHK1 inhibitor 1234015-52-1
MK-8776 (SCH-900776) Chk1 inhibitor,potent and selective 891494-63-6
NSC 109555 ditosylate 66748-43-4
PF-477736 Chk1 inhibitor 952021-60-2
SCH900776 S-isomer Checkpoint kinase(Chk)inhibitor 891494-64-7
TCS 2312 838823-31-7
genes like me logo Genes that share compounds with CHEK1: view

Drug products for research

Transcripts for CHEK1 Gene

mRNA/cDNA for CHEK1 Gene

6 REFSEQ mRNAs :
27 NCBI additional mRNA sequence :
15 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CHEK1

Clone products for research

  • Addgene plasmids for CHEK1

Alternative Splicing Database (ASD) splice patterns (SP) for CHEK1 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b · 2c ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7a · 7b ^ 8 ^ 9a · 9b · 9c · 9d ^ 10a · 10b ^ 11 ^ 12 ^ 13a · 13b ^ 14 ^
SP1: -
SP2: -
SP3: - - - - - -
SP4:
SP5: - - - - - - -
SP6: - - - - -
SP7: -
SP8: -
SP9: - -
SP10: - -
SP11:

ExUns: 15 ^ 16
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:

Relevant External Links for CHEK1 Gene

GeneLoc Exon Structure for
CHEK1

Expression for CHEK1 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for CHEK1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for CHEK1 Gene

This gene is overexpressed in Breast (62.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for CHEK1 Gene



Protein tissue co-expression partners for CHEK1 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for CHEK1

SOURCE GeneReport for Unigene cluster for CHEK1 Gene:

Hs.24529

mRNA Expression by UniProt/SwissProt for CHEK1 Gene:

O14757-CHK1_HUMAN
Tissue specificity: Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.

Evidence on tissue expression from TISSUES for CHEK1 Gene

  • Nervous system(4.7)
  • Bone marrow(4.5)
  • Muscle(4.3)
  • Intestine(3)
  • Skin(2.5)
  • Blood(2.5)
  • Lymph node(2.4)
  • Liver(2.4)
  • Lung(2.3)
  • Kidney(2.3)
  • Bone(2.2)
genes like me logo Genes that share expression patterns with CHEK1: view

Primer products for research

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for CHEK1 Gene

Orthologs for CHEK1 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for CHEK1 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia CHEK1 29 30
  • 99.72 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia CHEK1 30
  • 93 (a)
OneToOne
Dog
(Canis familiaris)
Mammalia CHEK1 29 30
  • 92.95 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia CHEK1 29 30
  • 92.51 (n)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia CHEK1 30
  • 90 (a)
OneToOne
Mouse
(Mus musculus)
Mammalia Chek1 29 16 30
  • 89.01 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Chek1 29
  • 88.59 (n)
Chicken
(Gallus gallus)
Aves STT3A 29 30
  • 69.83 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia CHEK1 30
  • 82 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia chek1 29
  • 71.19 (n)
African clawed frog
(Xenopus laevis)
Amphibia LOC398191 29
Zebrafish
(Danio rerio)
Actinopterygii chek1 29 30
  • 63.88 (n)
OneToOne
efnb1 29
Rainbow Trout
(Oncorhynchus mykiss)
Actinopterygii Omy.1073 29
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP008118 29
  • 54.67 (n)
Fruit Fly
(Drosophila melanogaster)
Insecta grp 29 30 31
  • 53.82 (n)
OneToOne
Worm
(Caenorhabditis elegans)
Secernentea chk-1 29 30
  • 47.8 (n)
OneToMany
DC2.7b 31
  • 38 (a)
R02C2.2 31
  • 36 (a)
DC2.7a 31
  • 33 (a)
Y43D4A.6 30 31
  • 32 (a)
OneToMany
R02C2.1 30 31
  • 30 (a)
OneToMany
kin-34 30
  • 30 (a)
OneToMany
R02C2.6 30
  • 30 (a)
OneToMany
kin-33 30
  • 18 (a)
OneToMany
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes CHK1 29 30 32
  • 45.68 (n)
OneToOne
K. Lactis Yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0F01276g 29
  • 44.84 (n)
A. gosspyii yeast
(Eremothecium gossypii)
Saccharomycetes AGOS_AEL185C 29
  • 44.76 (n)
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 30
  • 52 (a)
OneToOne
Bread mold
(Neurospora crassa)
Ascomycetes NCU08346 29
  • 47.47 (n)
Fission Yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes chk1 29
  • 45.82 (n)
Sea Vase
(Ciona intestinalis)
Ascidiacea Cin.1196 29
Species where no ortholog for CHEK1 was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for CHEK1 Gene

ENSEMBL:
Gene Tree for CHEK1 (if available)
TreeFam:
Gene Tree for CHEK1 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for CHEK1: view image
Alliance of Genome Resources:
Additional Orthologs for CHEK1

Paralogs for CHEK1 Gene

(73) SIMAP similar genes for CHEK1 Gene using alignment to 10 proteins:

  • CHK1_HUMAN
  • E7EPP6_HUMAN
  • E9PJI4_HUMAN
  • E9PKQ3_HUMAN
  • E9PM65_HUMAN
  • E9PPA5_HUMAN
  • E9PQW7_HUMAN
  • E9PRU7_HUMAN
  • J3KN87_HUMAN
  • Q6W8P8_HUMAN
genes like me logo Genes that share paralogs with CHEK1: view

No data available for Paralogs for CHEK1 Gene

Variants for CHEK1 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for CHEK1 Gene

SNP ID Clinical significance and condition Chr 11 pos Variation AA Info Type
rs114579795 Likely Benign: Hereditary cancer-predisposing syndrome 125,629,059(+) C/T
NM_001114121.2(CHEK1):c.290-173C>T
INTRON
rs139144317 Likely Benign: Hereditary cancer-predisposing syndrome 125,654,744(+) T/C
NM_001114121.2(CHEK1):c.1336-481T>C
INTRON
rs141004252 Likely Benign: Hereditary cancer-predisposing syndrome 125,627,913(+) T/G
NM_001114121.2(CHEK1):c.289+83T>G
INTRON
rs141332865 Benign: not provided 125,653,772(+) A/G
NM_001114121.2(CHEK1):c.1260A>G (p.Arg420=)
NON_CODING_TRANSCRIPT_VARIANT,SYNONYMOUS_VARIANT,INTRON
rs1555067411 Uncertain Significance: not provided 125,627,626(+) A/G
NM_001114121.2(CHEK1):c.85A>G (p.Arg29Gly)
MISSENSE_VARIANT,NON_CODING_TRANSCRIPT_VARIANT,INTRON

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for CHEK1 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for CHEK1 Gene

Variant ID Type Subtype PubMed ID
esv32964 CNV gain+loss 17666407
nsv474534 CNV novel sequence insertion 20440878

Variation tolerance for CHEK1 Gene

Residual Variation Intolerance Score: 11.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.89; 67.55% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for CHEK1 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
CHEK1
Leiden Open Variation Database (LOVD)
CHEK1

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CHEK1 Gene

Disorders for CHEK1 Gene

MalaCards: The human disease database

(23) MalaCards diseases for CHEK1 Gene - From: COP and GCD

Disorder Aliases PubMed IDs
ataxia-telangiectasia
  • at
li-fraumeni syndrome
  • lfs
fanconi anemia, complementation group a
  • fanca
breast cancer
  • breast cancer, familial
retinoblastoma
  • rb1
- elite association - COSMIC cancer census association via MalaCards
Search CHEK1 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for CHEK1

Human Genome Epidemiology Navigator
(HuGE)
Atlas
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
Open Targets Platform
genes like me logo Genes that share disorders with CHEK1: view

No data available for UniProtKB/Swiss-Prot and Genatlas for CHEK1 Gene

Publications for CHEK1 Gene

  1. Atm-dependent interactions of a mammalian chk1 homolog with meiotic chromosomes. (PMID: 9382850) Flaggs G … Carr AM (Current biology : CB 1997) 2 3 4 22
  2. Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25. (PMID: 9278511) Sanchez Y … Elledge SJ (Science (New York, N.Y.) 1997) 2 3 4 22
  3. NEK11: linking CHK1 and CDC25A in DNA damage checkpoint signaling. (PMID: 20090422) Sørensen CS … Helin K (Cell cycle (Georgetown, Tex.) 2010) 3 4 22
  4. Tumor suppressor protein C53 antagonizes checkpoint kinases to promote cyclin-dependent kinase 1 activation. (PMID: 19223857) Jiang H … Li H (Cell research 2009) 3 4 22
  5. Human FEM1B is required for Rad9 recruitment and CHK1 activation in response to replication stress. (PMID: 19330022) Sun TP … Shieh SY (Oncogene 2009) 3 4 22

Products for CHEK1 Gene