Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent ma... See more...

Aliases for CHAF1B Gene

Aliases for CHAF1B Gene

  • Chromatin Assembly Factor 1 Subunit B 2 3 4 5
  • M-Phase Phosphoprotein 7 2 3 4
  • MPHOSPH7 2 3 4
  • CAF1P60 2 3 4
  • CAF1A 2 3 4
  • MPP7 2 3 4
  • Human Chromatin Assembly Factor-I P60 Subunit 2 3
  • Chromatin Assembly Factor I P60 Subunit 3 4
  • CAF-I 60 KDa Subunit 3 4
  • CAF-1 Subunit B 3 4
  • CAF-I P60 3 4
  • CAF-1 2 3
  • CAF1 2 3
  • Chromatin Assembly Factor 1, Subunit B (P60) 2
  • Chromatin Assembly Factor I, P60 Subunit 2
  • CAF-IP60 3
  • CHAF1B 5

External Ids for CHAF1B Gene

Previous GeneCards Identifiers for CHAF1B Gene

  • GC21P034335
  • GC21P034418
  • GC21P036678
  • GC21P036679
  • GC21P037757
  • GC21P023233

Summaries for CHAF1B Gene

Entrez Gene Summary for CHAF1B Gene

  • Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008]

GeneCards Summary for CHAF1B Gene

CHAF1B (Chromatin Assembly Factor 1 Subunit B) is a Protein Coding gene. Diseases associated with CHAF1B include Brain Cancer. Gene Ontology (GO) annotations related to this gene include chromatin binding and unfolded protein binding.

UniProtKB/Swiss-Prot Summary for CHAF1B Gene

  • Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.

Gene Wiki entry for CHAF1B Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for CHAF1B Gene

Genomics for CHAF1B Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for CHAF1B Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around CHAF1B on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for CHAF1B

Top Transcription factor binding sites by QIAGEN in the CHAF1B gene promoter:
  • E2F
  • E2F-1
  • E47
  • FAC1
  • FOXI1
  • HFH-3
  • LCR-F1
  • Nkx2-2
  • STAT5A
  • Tal-1beta

Genomic Locations for CHAF1B Gene

Genomic Locations for CHAF1B Gene
chr21:36,380,145-36,419,015
(GRCh38/hg38)
Size:
38,871 bases
Orientation:
Plus strand
chr21:37,757,676-37,791,313
(GRCh37/hg19)
Size:
33,638 bases
Orientation:
Plus strand

Genomic View for CHAF1B Gene

Genes around CHAF1B on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CHAF1B Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CHAF1B Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CHAF1B Gene

Proteins for CHAF1B Gene

  • Protein details for CHAF1B Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q13112-CAF1B_HUMAN
    Recommended name:
    Chromatin assembly factor 1 subunit B
    Protein Accession:
    Q13112
    Secondary Accessions:
    • Q99548

    Protein attributes for CHAF1B Gene

    Size:
    559 amino acids
    Molecular mass:
    61493 Da
    Quaternary structure:
    • Subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. In G2 and S phase also monomeric CHAF1B is detected.

neXtProt entry for CHAF1B Gene

Post-translational modifications for CHAF1B Gene

  • Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
  • Ubiquitination at Lys211, Lys234, Lys385, and Lys494
  • Modification sites at PhosphoSitePlus

Other Protein References for CHAF1B Gene

No data available for DME Specific Peptides for CHAF1B Gene

Domains & Families for CHAF1B Gene

Gene Families for CHAF1B Gene

HGNC:
Human Protein Atlas (HPA):
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for CHAF1B Gene

InterPro:
Blocks:
  • G-protein beta WD-40 repeat signature
  • Beta G-protein (transducin) signature
ProtoNet:

Suggested Antigen Peptide Sequences for CHAF1B Gene

GenScript: Design optimal peptide antigens:
  • M-phase phosphoprotein 7 (CAF1B_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q13112

UniProtKB/Swiss-Prot:

CAF1B_HUMAN :
  • Belongs to the WD repeat HIR1 family.
Family:
  • Belongs to the WD repeat HIR1 family.
genes like me logo Genes that share domains with CHAF1B: view

Function for CHAF1B Gene

Molecular function for CHAF1B Gene

UniProtKB/Swiss-Prot Function:
Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.
GENATLAS Biochemistry:
chromatin assembly factor 1 subunit B,p60,histone chaperone assembling nucleosomes,on replicating DNA

Phenotypes From GWAS Catalog for CHAF1B Gene

Gene Ontology (GO) - Molecular Function for CHAF1B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003682 chromatin binding TAS 7600578
GO:0005515 protein binding IPI 16980972
GO:0042393 histone binding NAS 7600578
GO:0051082 unfolded protein binding TAS 7600578
genes like me logo Genes that share ontologies with CHAF1B: view
genes like me logo Genes that share phenotypes with CHAF1B: view

Animal Model Products

CRISPR Products

miRNA for CHAF1B Gene

miRTarBase miRNAs that target CHAF1B

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CHAF1B

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for CHAF1B Gene

Localization for CHAF1B Gene

Subcellular locations from UniProtKB/Swiss-Prot for CHAF1B Gene

Nucleus. Cytoplasm. Note=DNA replication foci. Cytoplasmic in M phase.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CHAF1B gene
Compartment Confidence
nucleus 5
cytosol 2
extracellular 1
cytoskeleton 1
mitochondrion 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for CHAF1B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000790 nuclear chromatin IDA 14718166
GO:0005634 nucleus IBA,NAS 9614144
GO:0005654 nucleoplasm HDA,IDA --
GO:0005737 cytoplasm NAS 9614144
GO:0005829 colocalizes_with cytosol HDA 16780588
genes like me logo Genes that share ontologies with CHAF1B: view

Pathways & Interactions for CHAF1B Gene

PathCards logo

SuperPathways for CHAF1B Gene

No Data Available

SIGNOR curated interactions for CHAF1B Gene

Inactivates:

Gene Ontology (GO) - Biological Process for CHAF1B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006260 DNA replication IEA --
GO:0006281 DNA repair IEA --
GO:0006334 nucleosome assembly IBA 21873635
GO:0006335 DNA replication-dependent nucleosome assembly IDA 14718166
GO:0006974 cellular response to DNA damage stimulus IEA --
genes like me logo Genes that share ontologies with CHAF1B: view

No data available for Pathways by source for CHAF1B Gene

Drugs & Compounds for CHAF1B Gene

No Compound Related Data Available

Transcripts for CHAF1B Gene

mRNA/cDNA for CHAF1B Gene

1 REFSEQ mRNAs :
8 NCBI additional mRNA sequence :
3 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CHAF1B

Alternative Splicing Database (ASD) splice patterns (SP) for CHAF1B Gene

No ASD Table

Relevant External Links for CHAF1B Gene

GeneLoc Exon Structure for
CHAF1B

Expression for CHAF1B Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for CHAF1B Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for CHAF1B Gene

This gene is overexpressed in Muscle - Skeletal (x6.6).

Protein differential expression in normal tissues from HIPED for CHAF1B Gene

This gene is overexpressed in Lung (31.2), Testis (18.5), and Ovary (8.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for CHAF1B Gene



Protein tissue co-expression partners for CHAF1B Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for CHAF1B

SOURCE GeneReport for Unigene cluster for CHAF1B Gene:

Hs.75238

Evidence on tissue expression from TISSUES for CHAF1B Gene

  • Blood(4.2)
genes like me logo Genes that share expression patterns with CHAF1B: view

No data available for mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for CHAF1B Gene

Orthologs for CHAF1B Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for CHAF1B Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia CHAF1B 30 31
  • 99.52 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia CHAF1B 30 31
  • 86.96 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia CHAF1B 30 31
  • 83.48 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Chaf1b 30
  • 82.02 (n)
Mouse
(Mus musculus)
Mammalia Chaf1b 30 17 31
  • 81.84 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia CHAF1B 31
  • 78 (a)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia CHAF1B 31
  • 76 (a)
OneToOne
Chicken
(Gallus gallus)
Aves CHAF1B 30 31
  • 74.07 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia CHAF1B 31
  • 71 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia chaf1b 30
  • 66.98 (n)
African clawed frog
(Xenopus laevis)
Amphibia Xl.7781 30
Zebrafish
(Danio rerio)
Actinopterygii chaf1b 30 31
  • 74.02 (n)
OneToOne
Dr.3522 30
Fruit Fly
(Drosophila melanogaster)
Insecta Caf1-105 30 31 32
  • 55.18 (n)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP007544 30
  • 51.16 (n)
Worm
(Caenorhabditis elegans)
Secernentea chaf-2 31
  • 34 (a)
OneToOne
Y71G12B.1a 32
  • 33 (a)
Y71G12B.1b 32
  • 33 (a)
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes CAC2 31 33
  • 34 (a)
OneToOne
Thale Cress
(Arabidopsis thaliana)
eudicotyledons FAS2 30
  • 53.13 (n)
Rice
(Oryza sativa)
Liliopsida Os08g0108200 30
  • 53.13 (n)
Barley
(Hordeum vulgare)
Liliopsida Hv.11475 30
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 49 (a)
OneToMany
-- 31
  • 32 (a)
OneToMany
Species where no ortholog for CHAF1B was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for CHAF1B Gene

ENSEMBL:
Gene Tree for CHAF1B (if available)
TreeFam:
Gene Tree for CHAF1B (if available)
Aminode:
Evolutionary constrained regions (ECRs) for CHAF1B: view image

Paralogs for CHAF1B Gene

Pseudogenes.org Pseudogenes for CHAF1B Gene

genes like me logo Genes that share paralogs with CHAF1B: view

No data available for Paralogs for CHAF1B Gene

Variants for CHAF1B Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for CHAF1B Gene

SNP ID Clinical significance and condition Chr 21 pos Variation AA Info Type
716510 Benign: not provided 36,415,321(+) C/T MISSENSE_VARIANT
728908 Benign: not provided 36,413,280(+) G/A SYNONYMOUS_VARIANT
730362 Benign: not provided 36,413,066(+) C/T MISSENSE_VARIANT
778907 Benign: not provided 36,415,317(+) A/C MISSENSE_VARIANT
rs140630794 Likely Pathogenic: Intellectual disability; Global developmental delay; Attention deficit hyperactivity disorder 36,397,429(+) A/G MISSENSE_VARIANT

Additional dbSNP identifiers (rs#s) for CHAF1B Gene

Structural Variations from Database of Genomic Variants (DGV) for CHAF1B Gene

Variant ID Type Subtype PubMed ID
esv33578 CNV loss 17666407
nsv1064194 CNV gain 25217958

Variation tolerance for CHAF1B Gene

Residual Variation Intolerance Score: 22.3% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.87; 67.40% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for CHAF1B Gene

Human Gene Mutation Database (HGMD)
CHAF1B
SNPedia medical, phenotypic, and genealogical associations of SNPs for
CHAF1B

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CHAF1B Gene

Disorders for CHAF1B Gene

MalaCards: The human disease database

(1) MalaCards diseases for CHAF1B Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
brain cancer
  • adult brain tumor
- elite association - COSMIC cancer census association via MalaCards
genes like me logo Genes that share disorders with CHAF1B: view

No data available for UniProtKB/Swiss-Prot and Genatlas for CHAF1B Gene

Publications for CHAF1B Gene

  1. Nucleosome assembly activity and intracellular localization of human CAF-1 changes during the cell division cycle. (PMID: 9614144) Marheineke K … Krude T (The Journal of biological chemistry 1998) 3 4 23
  2. The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication. (PMID: 7600578) Kaufman PD … Stillman B (Cell 1995) 2 3 4
  3. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PMID: 17081983) Olsen JV … Mann M (Cell 2006) 3 4
  4. New histone incorporation marks sites of UV repair in human cells. (PMID: 17081972) Polo SE … Almouzni G (Cell 2006) 3 23
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4

Products for CHAF1B Gene

Sources for CHAF1B Gene