Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM, Mar 2008] See more...

Aliases for CHAF1A Gene

Aliases for CHAF1A Gene

  • Chromatin Assembly Factor 1 Subunit A 2 3 4 5
  • CAF1P150 2 3 4
  • Chromatin Assembly Factor I P150 Subunit 3 4
  • Chromatin Assembly Factor I (150 KDa) 2 3
  • CAF-I 150 KDa Subunit 3 4
  • CAF-1 Subunit A 3 4
  • CAF-I P150 3 4
  • CAF-1 2 3
  • CAF1B 2 3
  • Hp150 3 4
  • CAF1 2 3
  • P150 2 3
  • Chromatin Assembly Factor 1, Subunit A (P150) 2
  • CTB-50L17.7 3
  • MGC71229 2
  • CHAF1A 5
  • CAF 4

External Ids for CHAF1A Gene

Previous GeneCards Identifiers for CHAF1A Gene

  • GC19P004494
  • GC19P004342
  • GC19P004353
  • GC19P004165

Summaries for CHAF1A Gene

Entrez Gene Summary for CHAF1A Gene

  • Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM, Mar 2008]

GeneCards Summary for CHAF1A Gene

CHAF1A (Chromatin Assembly Factor 1 Subunit A) is a Protein Coding gene. Diseases associated with CHAF1A include Prostate Squamous Cell Carcinoma and Alpha Thalassemia-Intellectual Disability Syndrome Type 1. Among its related pathways are Chromatin Regulation / Acetylation and Nanoparticle triggered autophagic cell death. Gene Ontology (GO) annotations related to this gene include identical protein binding and unfolded protein binding.

UniProtKB/Swiss-Prot Summary for CHAF1A Gene

  • Core component of the CAF-1 complex, a complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.

Gene Wiki entry for CHAF1A Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for CHAF1A Gene

Genomics for CHAF1A Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for CHAF1A Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around CHAF1A on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for CHAF1A

Top Transcription factor binding sites by QIAGEN in the CHAF1A gene promoter:
  • AML1a
  • AP-1
  • c-Fos

Genomic Locations for CHAF1A Gene

Genomic Locations for CHAF1A Gene
chr19:4,402,596-4,448,322
(GRCh38/hg38)
Size:
45,727 bases
Orientation:
Plus strand
chr19:4,402,659-4,445,015
(GRCh37/hg19)
Size:
42,357 bases
Orientation:
Plus strand

Genomic View for CHAF1A Gene

Genes around CHAF1A on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CHAF1A Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CHAF1A Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CHAF1A Gene

Proteins for CHAF1A Gene

  • Protein details for CHAF1A Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q13111-CAF1A_HUMAN
    Recommended name:
    Chromatin assembly factor 1 subunit A
    Protein Accession:
    Q13111
    Secondary Accessions:
    • Q6NXG5
    • Q7Z7K3
    • Q9UJY8

    Protein attributes for CHAF1A Gene

    Size:
    956 amino acids
    Molecular mass:
    106910 Da
    Quaternary structure:
    • Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. CHAF1A binds directly to PCNA and to CBX1. Interacts with MBD1 (PubMed:12697822). Interacts directly with CBX5 via the PxVxL motif. Interacts with CBX5.
    SequenceCaution:
    • Sequence=AAA76736.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305}; Sequence=AAF04291.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305}; Sequence=AAH52620.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305}; Sequence=AAH52620.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence starting in position 426.; Evidence={ECO:0000305};

    Alternative splice isoforms for CHAF1A Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for CHAF1A Gene

Post-translational modifications for CHAF1A Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for CHAF1A Gene

No data available for DME Specific Peptides for CHAF1A Gene

Domains & Families for CHAF1A Gene

Gene Families for CHAF1A Gene

Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for CHAF1A Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for CHAF1A Gene

GenScript: Design optimal peptide antigens:
  • Chromatin assembly factor I p150 subunit (CAF1A_HUMAN)
  • Chromatin assembly factor 1, subunit A (P150), isoform CRA_a (D6W625_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q13111

UniProtKB/Swiss-Prot:

CAF1A_HUMAN :
  • Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
  • Belongs to the CHAF1A family.
Domain:
  • Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
Family:
  • Belongs to the CHAF1A family.
genes like me logo Genes that share domains with CHAF1A: view

Function for CHAF1A Gene

Molecular function for CHAF1A Gene

UniProtKB/Swiss-Prot Function:
Core component of the CAF-1 complex, a complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.

Phenotypes From GWAS Catalog for CHAF1A Gene

Gene Ontology (GO) - Molecular Function for CHAF1A Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003682 chromatin binding TAS 7600578
GO:0005515 protein binding IPI 15805117
GO:0042802 identical protein binding IPI 16826239
GO:0051082 unfolded protein binding TAS 7600578
GO:0070087 chromo shadow domain binding IPI 15882967
genes like me logo Genes that share ontologies with CHAF1A: view
genes like me logo Genes that share phenotypes with CHAF1A: view

Animal Models for CHAF1A Gene

MGI Knock Outs for CHAF1A:

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CHAF1A

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for CHAF1A Gene

Localization for CHAF1A Gene

Subcellular locations from UniProtKB/Swiss-Prot for CHAF1A Gene

Nucleus. Note=DNA replication foci.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CHAF1A gene
Compartment Confidence
nucleus 5
plasma membrane 1
extracellular 1
cytoskeleton 1
mitochondrion 1
cytosol 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nucleoplasm (3)
  • Cytosol (1)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for CHAF1A Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000790 nuclear chromatin IDA 14718166
GO:0005634 nucleus IBA 21873635
GO:0032991 protein-containing complex IDA 14718166
GO:0033186 CAF-1 complex IBA,IDA 8858152
genes like me logo Genes that share ontologies with CHAF1A: view

Pathways & Interactions for CHAF1A Gene

genes like me logo Genes that share pathways with CHAF1A: view

Pathways by source for CHAF1A Gene

1 BioSystems pathway for CHAF1A Gene
1 Cell Signaling Technology pathway for CHAF1A Gene

SIGNOR curated interactions for CHAF1A Gene

Inactivates:

Gene Ontology (GO) - Biological Process for CHAF1A Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006260 DNA replication IEA --
GO:0006281 DNA repair IEA --
GO:0006334 nucleosome assembly IBA 21873635
GO:0006335 DNA replication-dependent nucleosome assembly IDA 14718166
GO:0006974 cellular response to DNA damage stimulus IEA --
genes like me logo Genes that share ontologies with CHAF1A: view

Drugs & Compounds for CHAF1A Gene

No Compound Related Data Available

Transcripts for CHAF1A Gene

mRNA/cDNA for CHAF1A Gene

1 REFSEQ mRNAs :
12 NCBI additional mRNA sequence :
7 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CHAF1A

Alternative Splicing Database (ASD) splice patterns (SP) for CHAF1A Gene

ExUns: 1a · 1b ^ 2 ^ 3a · 3b · 3c · 3d ^ 4 ^ 5a · 5b ^ 6a · 6b · 6c ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14a · 14b ^ 15
SP1: - - -
SP2:
SP3:
SP4: - -
SP5:

Relevant External Links for CHAF1A Gene

GeneLoc Exon Structure for
CHAF1A

Expression for CHAF1A Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for CHAF1A Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for CHAF1A Gene

This gene is overexpressed in Adipocyte (44.7) and Testis (14.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for CHAF1A Gene



Protein tissue co-expression partners for CHAF1A Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for CHAF1A

SOURCE GeneReport for Unigene cluster for CHAF1A Gene:

Hs.79018

Evidence on tissue expression from TISSUES for CHAF1A Gene

  • Nervous system(4.3)
  • Liver(2.3)
  • Skin(2.2)
genes like me logo Genes that share expression patterns with CHAF1A: view

No data available for mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for CHAF1A Gene

Orthologs for CHAF1A Gene

This gene was present in the common ancestor of animals.

Orthologs for CHAF1A Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia CHAF1A 30 31
  • 98.99 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia CHAF1A 30 31
  • 83.05 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia CHAF1A 30 31
  • 80.77 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Chaf1a 30
  • 71.79 (n)
Mouse
(Mus musculus)
Mammalia Chaf1a 30 17 31
  • 71.38 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia CHAF1A 31
  • 62 (a)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia CHAF1A 31
  • 34 (a)
OneToOne
Chicken
(Gallus gallus)
Aves CHAF1A 30 31
  • 65.14 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia CHAF1A 31
  • 54 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia LOC100485264 30
  • 60.98 (n)
Str.1170 30
African clawed frog
(Xenopus laevis)
Amphibia LOC398222 30
Zebrafish
(Danio rerio)
Actinopterygii chaf1a 31
  • 45 (a)
OneToOne
Rainbow Trout
(Oncorhynchus mykiss)
Actinopterygii Omy.5956 30
Fruit Fly
(Drosophila melanogaster)
Insecta Caf1-180 31
  • 19 (a)
OneToOne
Worm
(Caenorhabditis elegans)
Secernentea chaf-1 31
  • 26 (a)
OneToOne
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 42 (a)
OneToOne
Sea Vase
(Ciona intestinalis)
Ascidiacea Cin.1879 30
Species where no ortholog for CHAF1A was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for CHAF1A Gene

ENSEMBL:
Gene Tree for CHAF1A (if available)
TreeFam:
Gene Tree for CHAF1A (if available)
Aminode:
Evolutionary constrained regions (ECRs) for CHAF1A: view image

Paralogs for CHAF1A Gene

Pseudogenes.org Pseudogenes for CHAF1A Gene

genes like me logo Genes that share paralogs with CHAF1A: view

No data available for Paralogs for CHAF1A Gene

Variants for CHAF1A Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for CHAF1A Gene

SNP ID Clinical significance and condition Chr 19 pos Variation AA Info Type
738314 Likely Benign: not provided 4,442,916(+) G/A INTRON_VARIANT
781378 Benign: not provided 4,409,009(+) C/G SYNONYMOUS_VARIANT
rs2230635 - p.Met239Val
rs243383 - p.Ala950Ser
rs35651457 - p.Asp167Val

Additional dbSNP identifiers (rs#s) for CHAF1A Gene

Structural Variations from Database of Genomic Variants (DGV) for CHAF1A Gene

Variant ID Type Subtype PubMed ID
esv3555904 CNV deletion 23714750
nsv476449 CNV novel sequence insertion 20440878
nsv833718 CNV loss 17160897
nsv833720 CNV loss 17160897

Variation tolerance for CHAF1A Gene

Residual Variation Intolerance Score: 16.3% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.96; 59.86% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for CHAF1A Gene

Human Gene Mutation Database (HGMD)
CHAF1A
SNPedia medical, phenotypic, and genealogical associations of SNPs for
CHAF1A

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CHAF1A Gene

Disorders for CHAF1A Gene

MalaCards: The human disease database

(3) MalaCards diseases for CHAF1A Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
prostate squamous cell carcinoma
  • squamous cell carcinoma of prostate
alpha thalassemia-intellectual disability syndrome type 1
  • alpha-thalassemia/mental retardation syndrome, deletion-type
digeorge syndrome
  • cayler cardiofacial syndrome
- elite association - COSMIC cancer census association via MalaCards

Additional Disease Information for CHAF1A

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
genes like me logo Genes that share disorders with CHAF1A: view

No data available for UniProtKB/Swiss-Prot and Genatlas for CHAF1A Gene

Publications for CHAF1A Gene

  1. The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication. (PMID: 7600578) Kaufman PD … Stillman B (Cell 1995) 2 3 4 23
  2. Genomic sequence and expression analyses of human chromatin assembly factor 1 p150 gene. (PMID: 11250073) Dong H … Huang GM (Gene 2001) 3 4 23
  3. Dimerization of the largest subunit of chromatin assembly factor 1: importance in vitro and during Xenopus early development. (PMID: 11296234) Quivy JP … Almouzni G (The EMBO journal 2001) 3 4 23
  4. A CAF-1-PCNA-mediated chromatin assembly pathway triggered by sensing DNA damage. (PMID: 10648606) Moggs JG … Almouzni G (Molecular and cellular biology 2000) 3 4 23
  5. CAF-1 and the inheritance of chromatin states: at the crossroads of DNA replication and repair. (PMID: 10893180) Ridgway P … Almouzni G (Journal of cell science 2000) 3 4 23

Products for CHAF1A Gene

Sources for CHAF1A Gene