CEBPB Gene (Protein Coding)

CCAAT Enhancer Binding Protein Beta

GC20P050190
GIFtS:
45
This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The... See more...

Aliases for CEBPB Gene

Aliases for CEBPB Gene

  • CCAAT Enhancer Binding Protein Beta 2 3 5
  • CCAAT/Enhancer Binding Protein (C/EBP), Beta 2 3
  • Interleukin 6-Dependent DNA-Binding Protein 2 3
  • CCAAT/Enhancer-Binding Protein Beta 3 4
  • Nuclear Factor Of Interleukin 6 2 3
  • Transcription Factor 5 3 4
  • Nuclear Factor NF-IL6 3 4
  • TCF5 3 4
  • Liver-Enriched Transcriptional Activator Protein 2
  • CCAAT/Enhancer Binding Protein Beta 2
  • Liver-Enriched Inhibitory Protein 4
  • Transcription Factor C/EBP Beta 3
  • Liver Activator Protein 4
  • C/EBP-Beta 3
  • C/EBP Beta 4
  • IL6DBP 3
  • NF-IL6 3
  • TCF-5 4
  • LAP 4
  • LIP 4

External Ids for CEBPB Gene

Previous HGNC Symbols for CEBPB Gene

  • TCF5

Previous GeneCards Identifiers for CEBPB Gene

  • GC20P048535
  • GC20P049445
  • GC20P049492
  • GC20P048240
  • GC20P048807
  • GC20P045555

Summaries for CEBPB Gene

Entrez Gene Summary for CEBPB Gene

  • This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. [provided by RefSeq, Oct 2013]

GeneCards Summary for CEBPB Gene

CEBPB (CCAAT Enhancer Binding Protein Beta) is a Protein Coding gene. Diseases associated with CEBPB include Alk-Positive Anaplastic Large Cell Lymphoma and Myxoid Liposarcoma. Among its related pathways are Signaling mediated by p38-alpha and p38-beta and IL4-mediated signaling events. Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and sequence-specific DNA binding. An important paralog of this gene is CEBPA.

UniProtKB/Swiss-Prot Summary for CEBPB Gene

  • Important transcription factor regulating the expression of genes involved in immune and inflammatory responses (PubMed:1741402, PubMed:9374525, PubMed:12048245, PubMed:18647749). Plays also a significant role in adipogenesis, as well as in the gluconeogenic pathway, liver regeneration, and hematopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Its functional capacity is governed by protein interactions and post-translational protein modifications. During early embryogenesis, plays essential and redundant functions with CEBPA. Has a promitotic effect on many cell types such as hepatocytes and adipocytes but has an antiproliferative effect on T-cells by repressing MYC expression, facilitating differentiation along the T-helper 2 lineage. Binds to regulatory regions of several acute-phase and cytokines genes and plays a role in the regulation of acute-phase reaction and inflammation. Plays also a role in intracellular bacteria killing (By similarity). During adipogenesis, is rapidly expressed and, after activation by phosphorylation, induces CEBPA and PPARG, which turn on the series of adipocyte genes that give rise to the adipocyte phenotype. The delayed transactivation of the CEBPA and PPARG genes by CEBPB appears necessary to allow mitotic clonal expansion and thereby progression of terminal differentiation (PubMed:20829347). Essential for female reproduction because of a critical role in ovarian follicle development (By similarity). Restricts osteoclastogenesis: together with NFE2L1; represses expression of DSPP during odontoblast differentiation (By similarity).
  • [Isoform 2]: Essential for gene expression induction in activated macrophages. Plays a major role in immune responses such as CD4(+) T-cell response, granuloma formation and endotoxin shock. Not essential for intracellular bacteria killing.
  • [Isoform 3]: Acts as a dominant negative through heterodimerization with isoform 2 (PubMed:11741938). Promotes osteoblast differentiation and osteoclastogenesis (By similarity).

Gene Wiki entry for CEBPB Gene

Additional gene information for CEBPB Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for CEBPB Gene

Genomics for CEBPB Gene

GeneHancer (GH) Regulatory Elements for CEBPB Gene

Promoters and enhancers for CEBPB Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around CEBPB on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for CEBPB

Top Transcription factor binding sites by QIAGEN in the CEBPB gene promoter:
  • c-Myb
  • CREB
  • deltaCREB
  • GR
  • GR-alpha
  • GR-beta

Genomic Locations for CEBPB Gene

Genomic Locations for CEBPB Gene
chr20:50,190,583-50,192,690
(GRCh38/hg38)
Size:
2,108 bases
Orientation:
Plus strand
chr20:48,807,376-48,809,212
(GRCh37/hg19)
Size:
1,837 bases
Orientation:
Plus strand

Genomic View for CEBPB Gene

Genes around CEBPB on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CEBPB Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CEBPB Gene
GeneLoc Logo Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for CEBPB Gene

Proteins for CEBPB Gene

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  • Protein details for CEBPB Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P17676-CEBPB_HUMAN
    Recommended name:
    CCAAT/enhancer-binding protein beta
    Protein Accession:
    P17676
    Secondary Accessions:
    • A8K671
    • Q96IH2
    • Q9H4Z5

    Protein attributes for CEBPB Gene

    Size:
    345 amino acids
    Molecular mass:
    36106 Da
    Quaternary structure:
    • Binds DNA as a homodimer and as a heterodimer (PubMed:11018027, PubMed:11257229, PubMed:11792321). Interacts with ATF4. Binds DNA as a heterodimer with ATF4 (PubMed:11018027). Interacts with MYB; within the complex, MYB and CEBPB bind to different promoter regions (PubMed:11792321). Can form stable heterodimers with CEBPD (PubMed:1741402). Can form stable heterodimers with CEBPA and CEBPE (By similarity). Isoform 2 and isoform 3 also form heterodimers. Interacts with TRIM28 and PTGES2. Interacts with PRDM16. Interacts with CCDC85B. Forms a complex with THOC5. Interacts with ZNF638; this interaction increases transcriptional activation. Interacts with CIDEA and CIDEC; these interactions increase transcriptional activation of a subset of CEBPB downstream target genes (By similarity). Interacts with DDIT3/CHOP (PubMed:20829347). Interacts with EP300; recruits EP300 to chromatin. Interacts with RORA; the interaction disrupts interaction with EP300. Interacts (not methylated) with MED23, MED26, SMARCA2, SMARCB1 and SMARCC1 (PubMed:20111005). Interacts with KAT2A and KAT2B (By similarity). Interacts with ATF5; EP300 is required for ATF5 and CEBPB interaction and DNA binding (By similarity). Interacts with NFE2L1; the heterodimer represses expression of DSPP during odontoblast differentiation (By similarity).

    Three dimensional structures from OCA and Proteopedia for CEBPB Gene

    Alternative splice isoforms for CEBPB Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for CEBPB Gene

Protein Expression for CEBPB Gene

See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB

Post-translational modifications for CEBPB Gene

  • Methylated. Methylation at Arg-3 by CARM1 and at Lys-43 by EHMT2 inhibit transactivation activity. Methylation is probably inhibited by phosphorylation at Thr-235.
  • Sumoylated by polymeric chains of SUMO2 or SUMO3 (PubMed:12810706). Sumoylation at Lys-174 is required for inhibition of T-cells proliferation. In adipocytes, sumoylation at Lys-174 by PIAS1 leads to ubiquitination and subsequent proteasomal degradation. Desumoylated by SENP2, which abolishes ubiquitination and stabilizes protein levels (By similarity).
  • Ubiquitinated, leading to proteasomal degradation.
  • Phosphorylated at Thr-235 by MAPK and CDK2, serves to prime phosphorylation at Thr-226 and Ser-231 by GSK3B and acquire DNA-binding as well as transactivation activities, required to induce adipogenesis. MAPK and CDK2 act sequentially to maintain Thr-235 in the primed phosphorylated state during mitotical cloning expansion and thereby progression of terminal differentiation. Phosphorylation at Thr-266 enhances transactivation activity. Phosphorylation at Ser-325 in response to calcium increases transactivation activity. Phosphorylated at Thr-235 by RPS6KA1 (PubMed:11684016).
  • O-glycosylated, glycosylation at Ser-227 and Ser-228 prevents phosphorylation on Thr-235, Ser-231 and Thr-226 and DNA binding activity which delays the adipocyte differentiation program.
  • Acetylated. Acetylation at Lys-43 is an important and dynamic regulatory event that contributes to its ability to transactivate target genes, including those associated with adipogenesis and adipocyte function. Deacetylation by HDAC1 represses its transactivation activity. Acetylated by KAT2A and KAT2B within a cluster of lysine residues between amino acids 129-133, this acetylation is strongly induced by glucocorticoid treatment and enhances transactivation activity.
  • Glycosylation at Ser227 and Ser228
  • Modification sites at PhosphoSitePlus

Other Protein References for CEBPB Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

No data available for DME Specific Peptides for CEBPB Gene

Domains & Families for CEBPB Gene

Gene Families for CEBPB Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins
  • Transcription factors

Protein Domains for CEBPB Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for CEBPB Gene

GenScript: Design optimal peptide antigens:
  • Transcription factor 5 (CEBPB_HUMAN)
  • CEBPB protein (Q9BSC0_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P17676

UniProtKB/Swiss-Prot:

CEBPB_HUMAN :
  • the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Belongs to the bZIP family. C/EBP subfamily.
Domain:
  • the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Family:
  • Belongs to the bZIP family. C/EBP subfamily.
genes like me logo Genes that share domains with CEBPB: view

Function for CEBPB Gene

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Molecular function for CEBPB Gene

UniProtKB/Swiss-Prot Function:
Important transcription factor regulating the expression of genes involved in immune and inflammatory responses (PubMed:1741402, PubMed:9374525, PubMed:12048245, PubMed:18647749). Plays also a significant role in adipogenesis, as well as in the gluconeogenic pathway, liver regeneration, and hematopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Its functional capacity is governed by protein interactions and post-translational protein modifications. During early embryogenesis, plays essential and redundant functions with CEBPA. Has a promitotic effect on many cell types such as hepatocytes and adipocytes but has an antiproliferative effect on T-cells by repressing MYC expression, facilitating differentiation along the T-helper 2 lineage. Binds to regulatory regions of several acute-phase and cytokines genes and plays a role in the regulation of acute-phase reaction and inflammation. Plays also a role in intracellular bacteria killing (By similarity). During adipogenesis, is rapidly expressed and, after activation by phosphorylation, induces CEBPA and PPARG, which turn on the series of adipocyte genes that give rise to the adipocyte phenotype. The delayed transactivation of the CEBPA and PPARG genes by CEBPB appears necessary to allow mitotic clonal expansion and thereby progression of terminal differentiation (PubMed:20829347). Essential for female reproduction because of a critical role in ovarian follicle development (By similarity). Restricts osteoclastogenesis: together with NFE2L1; represses expression of DSPP during odontoblast differentiation (By similarity).
UniProtKB/Swiss-Prot Function:
[Isoform 2]: Essential for gene expression induction in activated macrophages. Plays a major role in immune responses such as CD4(+) T-cell response, granuloma formation and endotoxin shock. Not essential for intracellular bacteria killing.
UniProtKB/Swiss-Prot Function:
[Isoform 3]: Acts as a dominant negative through heterodimerization with isoform 2 (PubMed:11741938). Promotes osteoblast differentiation and osteoclastogenesis (By similarity).
UniProtKB/Swiss-Prot Induction:
By ER stress.
GENATLAS Biochemistry:
CCAAT enhancer binding protein beta (C/EBP),expressed in preadipocytes and inducing,conjointly with CEBPD,the induction of CEBPA and PPARG,required for adipocyte differentiation.

Phenotypes From GWAS Catalog for CEBPB Gene

Gene Ontology (GO) - Molecular Function for CEBPB Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000977 RNA polymerase II regulatory region sequence-specific DNA binding IDA 7959007
GO:0000978 RNA polymerase II proximal promoter sequence-specific DNA binding IEA --
GO:0000979 RNA polymerase II core promoter sequence-specific DNA binding IEA --
GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific NAS,IEA 19274049
GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific IEA,ISS 15308669
genes like me logo Genes that share ontologies with CEBPB: view
genes like me logo Genes that share phenotypes with CEBPB: view

Animal Models for CEBPB Gene

MGI Knock Outs for CEBPB:

Animal Model Products

CRISPR Products

miRNA for CEBPB Gene

miRTarBase miRNAs that target CEBPB
Targeted motifs for CEBPB Gene
HOMER Transcription Factor Regulatory Elements motif CEBPB
  • Consensus sequence: ATTGCGCAAC Submotif: canonical Cell Type: ThioMac GEO ID: GSE21512

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CEBPB

Clone Products

  • Addgene plasmids for CEBPB

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology and Transcription Factor Targets for CEBPB Gene

Localization for CEBPB Gene

Subcellular locations from UniProtKB/Swiss-Prot for CEBPB Gene

Nucleus. Cytoplasm. Note=Translocates to the nucleus when phosphorylated at Ser-288. In T-cells when sumoylated drawn to pericentric heterochromatin thereby allowing proliferation (By similarity). {ECO:0000250 UniProtKB:P28033, ECO:0000269 PubMed:9374525}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CEBPB gene
Compartment Confidence
nucleus 5
cytosol 3
plasma membrane 2
extracellular 2
cytoskeleton 2
mitochondrion 2
peroxisome 2
endoplasmic reticulum 2
lysosome 2
endosome 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nucleoplasm (4)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for CEBPB Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000779 condensed chromosome, centromeric region IEA --
GO:0000790 nuclear chromatin ISA,IEA --
GO:0005634 nucleus IDA,TAS 20829347
GO:0005654 nucleoplasm TAS --
GO:0005737 cytoplasm IEA --
genes like me logo Genes that share ontologies with CEBPB: view

Pathways & Interactions for CEBPB Gene

PathCards logo

SuperPathways for CEBPB Gene

SuperPathway Contained pathways
1 IL-17 Family Signaling Pathways
IL-17 Family Signaling Pathways
.51
IL17 signaling pathway
.51
IL-17 signaling pathway
.33
2 Cellular Senescence (REACTOME)
Cellular Senescence
.64
Senescence-Associated Secretory Phenotype (SASP)
.56
Cellular responses to stress
.44
3 G-protein signaling Ras family GTPases in kinase cascades (scheme)
G-protein signaling Ras family GTPases in kinase cascades (scheme)
.44
Immune response Oncostatin M signaling via MAPK in human cells
.39
Oncostatin M Signaling Pathway
.35
4 Human Embryonic Stem Cell Pluripotency
Factors Promoting Cardiogenesis in Vertebrates
.50
Human Embryonic Stem Cell Pluripotency
.50
5 IL-2 Pathway
Prolactin Signaling
.54
Growth Hormone Signaling
.49
genes like me logo Genes that share pathways with CEBPB: view

Pathways by source for CEBPB Gene

1 Sino Biological pathway for CEBPB Gene
10 GeneGo (Thomson Reuters) pathways for CEBPB Gene
  • Development A2B receptor- action via G-protein alpha s
  • Development Growth hormone signaling via PI3K/AKT and MAPK cascades
  • Development Prolactin receptor signaling
  • Development_Glucocorticoid receptor signaling
  • G-protein signaling Ras family GTPases in kinase cascades (scheme)
10 Qiagen pathways for CEBPB Gene
  • Epithelial Adherens Junctions
  • Factors Promoting Cardiogenesis in Vertebrates
  • Glucocorticoid Receptor Signaling
  • Growth Hormone Signaling
  • Human Embryonic Stem Cell Pluripotency
2 Cell Signaling Technology pathways for CEBPB Gene

SIGNOR curated interactions for CEBPB Gene

Activates:
Inactivates:
Is activated by:
Is inactivated by:

Gene Ontology (GO) - Biological Process for CEBPB Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000122 negative regulation of transcription by RNA polymerase II ISS 15308669
GO:0001541 ovarian follicle development IEA,ISS --
GO:0001889 liver development IEA --
GO:0001892 embryonic placenta development IEA --
GO:0006355 regulation of transcription, DNA-templated IDA 20829347
genes like me logo Genes that share ontologies with CEBPB: view

Drugs & Compounds for CEBPB Gene

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(27) Drugs for CEBPB Gene - From: DrugBank and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Quercetin Experimental, Investigational Pharma Target, inhibitor Antitumor agent, Non-selective PI 3-kinase inhibitor 50

(23) Additional Compounds for CEBPB Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with CEBPB: view

Transcripts for CEBPB Gene

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mRNA/cDNA for CEBPB Gene

3 REFSEQ mRNAs :
42 NCBI additional mRNA sequence :
1 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CEBPB

Clone Products

  • Addgene plasmids for CEBPB

Alternative Splicing Database (ASD) splice patterns (SP) for CEBPB Gene

No ASD Table

Relevant External Links for CEBPB Gene

GeneLoc Exon Structure for
CEBPB

Expression for CEBPB Gene

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  1. Primer
  2. Gene Expression Assay

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for CEBPB Gene

mRNA expression in normal human tissues for CEBPB Gene
mRNA expression by GTEx for CEBPB GenemRNA expression by BioGPS for CEBPB Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for CEBPB Gene

This gene is overexpressed in Whole Blood (x8.9).

Protein differential expression in normal tissues from HIPED for CEBPB Gene

This gene is overexpressed in Liver (22.6), Placenta (20.0), Peripheral blood mononuclear cells (9.8), and Monocytes (7.9).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for CEBPB Gene



Protein tissue co-expression partners for CEBPB Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for CEBPB

SOURCE GeneReport for Unigene cluster for CEBPB Gene:

Hs.517106

mRNA Expression by UniProt/SwissProt for CEBPB Gene:

P17676-CEBPB_HUMAN
Tissue specificity: Expressed at low levels in the lung, kidney and spleen.

Evidence on tissue expression from TISSUES for CEBPB Gene

  • Heart(5)
  • Intestine(5)
  • Lung(5)
  • Muscle(4.7)
  • Skin(4.5)
  • Nervous system(4.3)
  • Liver(3.9)
  • Blood(3.5)
  • Bone marrow(2.6)
  • Spleen(2.3)
  • Pancreas(2.2)
  • Adrenal gland(2)
  • Thyroid gland(2)
genes like me logo Genes that share expression patterns with CEBPB: view

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for CEBPB Gene

Orthologs for CEBPB Gene

This gene was present in the common ancestor of animals.

Orthologs for CEBPB Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
 Mammalia CEBPB 31 30
  • 99.71 (n)
 OneToOne
dog
(Canis familiaris)
 Mammalia CEBPB 31 30
  • 96.71 (n)
 OneToOne
oppossum
(Monodelphis domestica)
 Mammalia CEBPB 31
  • 88 (a)
 OneToOne
mouse
(Mus musculus)
 Mammalia Cebpb 17 31 30
  • 83.9 (n)
rat
(Rattus norvegicus)
 Mammalia Cebpb 30
  • 82.83 (n)
chicken
(Gallus gallus)
 Aves CEBPB 31 30
  • 74.81 (n)
 OneToOne