CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytopla... See more...

Aliases for CDC25B Gene

Aliases for CDC25B Gene

  • Cell Division Cycle 25B 2 3 5
  • Dual Specificity Phosphatase Cdc25B 3 4
  • M-Phase Inducer Phosphatase 2 3 4
  • EC 3.1.3.48 4 51
  • CDC25HU2 4
  • CDC25B 5

External Ids for CDC25B Gene

Previous GeneCards Identifiers for CDC25B Gene

  • GC20P003765
  • GC20P003724
  • GC20P003771

Summaries for CDC25B Gene

Entrez Gene Summary for CDC25B Gene

  • CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]

GeneCards Summary for CDC25B Gene

CDC25B (Cell Division Cycle 25B) is a Protein Coding gene. Diseases associated with CDC25B include Breast Cancer and Esophageal Cancer. Among its related pathways are Cyclin A/B1 associated events during G2/M transition and Neurodegenerative Diseases. Gene Ontology (GO) annotations related to this gene include protein kinase binding and protein tyrosine phosphatase activity. An important paralog of this gene is CDC25A.

UniProtKB/Swiss-Prot Summary for CDC25B Gene

  • Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

Tocris Summary for CDC25B Gene

  • Cdc25 is a dual specificity phosphatase with three isoforms in mammalian cells - Cdc25A, B and C. Cdc25 activates cdk complexes that drive the cell cycle. Cdc25 is involved in the DNA damage checkpoints and is known as a key mediator of cell cycle progression.

Gene Wiki entry for CDC25B Gene

No data available for CIViC Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for CDC25B Gene

Genomics for CDC25B Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for CDC25B Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around CDC25B on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for CDC25B

Top Transcription factor binding sites by QIAGEN in the CDC25B gene promoter:
  • GR
  • GR-alpha

Genomic Locations for CDC25B Gene

Genomic Locations for CDC25B Gene
chr20:3,786,772-3,806,121
(GRCh38/hg38)
Size:
19,350 bases
Orientation:
Plus strand
chr20:3,767,578-3,786,762
(GRCh37/hg19)
Size:
19,185 bases
Orientation:
Plus strand

Genomic View for CDC25B Gene

Genes around CDC25B on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CDC25B Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CDC25B Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CDC25B Gene

Proteins for CDC25B Gene

  • Protein details for CDC25B Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P30305-MPIP2_HUMAN
    Recommended name:
    M-phase inducer phosphatase 2
    Protein Accession:
    P30305
    Secondary Accessions:
    • D3DVY1
    • D3DVY2
    • D3DVY3
    • D3DVY4
    • O43551
    • Q13971
    • Q5JX77
    • Q6RSS1
    • Q9BRA6

    Protein attributes for CDC25B Gene

    Size:
    580 amino acids
    Molecular mass:
    64987 Da
    Quaternary structure:
    • Interacts with MAPK14 and 14-3-3 proteins.

    Three dimensional structures from OCA and Proteopedia for CDC25B Gene

    Alternative splice isoforms for CDC25B Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for CDC25B Gene

Selected DME Specific Peptides for CDC25B Gene

P30305:
  • CEFSSERGPRMCR
  • FHCEFSS
  • LKGGYKEFF
  • DCRYPYEYEGGHIK
  • GKHQDLKY

Post-translational modifications for CDC25B Gene

  • Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
  • Ubiquitination at Lys71, Lys204, Lys398, and Lys469
  • Modification sites at PhosphoSitePlus

Antibody Products

  • Abcam antibodies for CDC25B

Domains & Families for CDC25B Gene

Gene Families for CDC25B Gene

HGNC:
Human Protein Atlas (HPA):
  • Cancer-related genes
  • Enzymes
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for CDC25B Gene

InterPro:
Blocks:
  • M-phase inducer phosphatase signature
  • Rhodanese-like
ProtoNet:

Suggested Antigen Peptide Sequences for CDC25B Gene

GenScript: Design optimal peptide antigens:
  • cDNA FLJ35394 fis, clone SKNSH2002768, highly similar to M-PHASE INDUCER PHOSPHATASE 2 (EC 3.1.3.48) (B3KS38_HUMAN)
  • cDNA FLJ53333, highly similar to M-phase inducer phosphatase 2 (EC 3.1.3.48) (B4DIG0_HUMAN)
  • Isoform CDC25B7 (E1YMX4_HUMAN)
  • Dual specificity phosphatase Cdc25B (MPIP2_HUMAN)
  • Cdc25B phosphatase (O43550_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P30305

UniProtKB/Swiss-Prot:

MPIP2_HUMAN :
  • Belongs to the MPI phosphatase family.
Family:
  • Belongs to the MPI phosphatase family.
genes like me logo Genes that share domains with CDC25B: view

Function for CDC25B Gene

Molecular function for CDC25B Gene

UniProtKB/Swiss-Prot Function:
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphate; Xref=Rhea:RHEA:10684, Rhea:RHEA-COMP:10136, Rhea:RHEA-COMP:10137, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:46858, ChEBI:CHEBI:82620; EC=3.1.3.48;.
UniProtKB/Swiss-Prot EnzymeRegulation:
Stimulated by B-type cyclins.
GENATLAS Biochemistry:
cdc25 protein phosphatase,non receptor type,complexing with CDC2 (M phase promoting factor (MPF) component),form B,regulator of the cell cycle

Enzyme Numbers (IUBMB) for CDC25B Gene

Phenotypes From GWAS Catalog for CDC25B Gene

Gene Ontology (GO) - Molecular Function for CDC25B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004721 phosphoprotein phosphatase activity TAS --
GO:0004725 protein tyrosine phosphatase activity IEA,TAS 9188863
GO:0005515 protein binding IPI 7644510
GO:0016787 hydrolase activity IEA --
GO:0019901 protein kinase binding IPI 12400006
genes like me logo Genes that share ontologies with CDC25B: view
genes like me logo Genes that share phenotypes with CDC25B: view

Animal Models for CDC25B Gene

MGI Knock Outs for CDC25B:

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CDC25B

Clone Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for CDC25B Gene

Localization for CDC25B Gene

Subcellular locations from UniProtKB/Swiss-Prot for CDC25B Gene

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for CDC25B gene
Compartment Confidence
cytoskeleton 5
nucleus 5
cytosol 5
plasma membrane 2
extracellular 2
mitochondrion 2
peroxisome 1
endoplasmic reticulum 1
endosome 1
lysosome 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Mitotic spindle (3)
  • Vesicles (3)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for CDC25B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000922 spindle pole IEA,IDA 15908796
GO:0005634 nucleus IBA 21873635
GO:0005654 nucleoplasm TAS --
GO:0005737 cytoplasm IBA 21873635
GO:0005813 centrosome IDA 15908796
genes like me logo Genes that share ontologies with CDC25B: view

Pathways & Interactions for CDC25B Gene

PathCards logo

SuperPathways for CDC25B Gene

SuperPathway Contained pathways
1 Cell cycle
2 MAPK signaling pathway
3 MAPK Erk Pathway
4 Cell cycle Role of 14-3-3 proteins in cell cycle regulation
.45
.45
5 Cell Cycle, Mitotic
genes like me logo Genes that share pathways with CDC25B: view

Pathways by source for CDC25B Gene

2 GeneGo (Thomson Reuters) pathways for CDC25B Gene
  • Cell cycle Cell cycle (generic schema)
  • Cell cycle Role of 14-3-3 proteins in cell cycle regulation
2 Qiagen pathways for CDC25B Gene
  • 14-3-3 and Cell Cycle Regulation
  • Molecular Mechanisms of Cancer
1 Cell Signaling Technology pathway for CDC25B Gene

SIGNOR curated interactions for CDC25B Gene

Activates:
Is activated by:
Is inactivated by:

Gene Ontology (GO) - Biological Process for CDC25B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000086 G2/M transition of mitotic cell cycle IBA,TAS 12400006
GO:0000278 mitotic cell cycle TAS 9188863
GO:0001556 oocyte maturation IEA --
GO:0006468 protein phosphorylation IDA 17332740
GO:0006470 protein dephosphorylation IEA --
genes like me logo Genes that share ontologies with CDC25B: view

Drugs & Compounds for CDC25B Gene

(10) Drugs for CDC25B Gene - From: DrugBank, ApexBio, HMDB, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Phosphoric acid Approved Pharma 0
Water Approved Pharma 0
Cysteic acid Experimental Pharma Target 0
NSC 663284 Pharma Potent, selective Cdc25 phosphatase inhibitor 0
NSC 95397 Pharma Selective Cdc25 dual specificity phosphatase inhibitor 0

(5) Additional Compounds for CDC25B Gene - From: Novoseek and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Phosphate
  • [po(OH)3]
  • Acide phosphorique
  • Acidum phosphoricum
  • H3PO4
  • Orthophosphoric acid
14066-19-4, 14265-44-2

(2) Tocris Compounds for CDC25B Gene

Compound Action Cas Number
NSC 663284 Potent, selective Cdc25 phosphatase inhibitor 383907-43-5
NSC 95397 Selective Cdc25 dual specificity phosphatase inhibitor 93718-83-3

(2) ApexBio Compounds for CDC25B Gene

Compound Action Cas Number
NSC 663284 383907-43-5
NSC 95397 93718-83-3
genes like me logo Genes that share compounds with CDC25B: view

Drug Products

Transcripts for CDC25B Gene

mRNA/cDNA for CDC25B Gene

12 REFSEQ mRNAs :
20 NCBI additional mRNA sequence :
9 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for CDC25B

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for CDC25B Gene

ExUns: 1a · 1b · 1c ^ 2a · 2b · 2c · 2d ^ 3a · 3b · 3c ^ 4a · 4b ^ 5a · 5b ^ 6 ^ 7 ^ 8a · 8b · 8c ^ 9a · 9b ^ 10 ^ 11a · 11b ^ 12a · 12b ·
SP1: - - - - - - - - - - -
SP2: - - - - - - - - - - - -
SP3: - - - - - - -
SP4: - - - - - - - -
SP5: - - - - - - - - -
SP6: - - -
SP7: - -
SP8: - - - -
SP9:
SP10: - - - - - -
SP11: - - -
SP12: - - - - -
SP13: - - -
SP14: - - -
SP15:

ExUns: 12c ^ 13 ^ 14 ^ 15 ^ 16 ^ 17a · 17b ^ 18 ^ 19 ^ 20a · 20b · 20c
SP1: -
SP2: -
SP3: -
SP4: -
SP5: -
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:
SP13:
SP14: - -
SP15:

Relevant External Links for CDC25B Gene

GeneLoc Exon Structure for
CDC25B

Expression for CDC25B Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for CDC25B Gene

Protein differential expression in normal tissues from HIPED for CDC25B Gene

This gene is overexpressed in Plasma (68.9).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for CDC25B Gene



Protein tissue co-expression partners for CDC25B Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for CDC25B

SOURCE GeneReport for Unigene cluster for CDC25B Gene:

Hs.153752

Evidence on tissue expression from TISSUES for CDC25B Gene

  • Nervous system(4.9)
  • Lung(3.6)
  • Intestine(3.1)
  • Skin(3)
  • Pancreas(2.6)
  • Lymph node(2.6)
  • Liver(2.3)
  • Blood(2.3)
  • Kidney(2.1)
  • Bone marrow(2.1)
  • Spleen(2.1)
  • Stomach(2)
genes like me logo Genes that share expression patterns with CDC25B: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for CDC25B Gene

Orthologs for CDC25B Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for CDC25B Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia CDC25B 30 31
  • 99.37 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia CDC25B 30 31
  • 87.97 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia CDC25B 30 31
  • 85.53 (n)
OneToOne
Mouse
(Mus musculus)
Mammalia Cdc25b 30 17 31
  • 81.33 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Cdc25b 30
  • 81.07 (n)
Platypus
(Ornithorhynchus anatinus)
Mammalia CDC25B 31
  • 52 (a)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia CDC25B 31
  • 54 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia cdc25b 30
  • 61.78 (n)
Zebrafish
(Danio rerio)
Actinopterygii cdc25 31
  • 45 (a)
OneToMany
Fruit Fly
(Drosophila melanogaster)
Insecta stg 31 32
  • 29 (a)
ManyToMany
twe 31 32
  • 29 (a)
ManyToMany
Worm
(Caenorhabditis elegans)
Secernentea cdc-25.3 31
  • 24 (a)
ManyToMany
cdc-25.2 31
  • 23 (a)
ManyToMany
cdc-25.4 31
  • 23 (a)
ManyToMany
cdc-25.1 31
  • 19 (a)
ManyToMany
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes MIH1 31
  • 16 (a)
OneToMany
Sea Squirt
(Ciona savignyi)
Ascidiacea CSA.1787 31
  • 37 (a)
OneToMany
Species where no ortholog for CDC25B was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Oppossum (Monodelphis domestica)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for CDC25B Gene

ENSEMBL:
Gene Tree for CDC25B (if available)
TreeFam:
Gene Tree for CDC25B (if available)
Aminode:
Evolutionary constrained regions (ECRs) for CDC25B: view image

Paralogs for CDC25B Gene

Paralogs for CDC25B Gene

(3) SIMAP similar genes for CDC25B Gene using alignment to 8 proteins:

  • MPIP2_HUMAN
  • B3KS38_HUMAN
  • B4DIG0_HUMAN
  • E1YMX4_HUMAN
  • G8JLH2_HUMAN
  • L8E819_HUMAN
  • O43550_HUMAN
  • Q7LDQ7_HUMAN
genes like me logo Genes that share paralogs with CDC25B: view

Variants for CDC25B Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for CDC25B Gene

SNP ID Clinical significance and condition Chr 20 pos Variation AA Info Type
rs11570019 - p.Glu548Lys

Additional dbSNP identifiers (rs#s) for CDC25B Gene

Structural Variations from Database of Genomic Variants (DGV) for CDC25B Gene

Variant ID Type Subtype PubMed ID
esv3385581 CNV insertion 20981092
nsv1071676 CNV deletion 25765185
nsv585285 CNV loss 21841781
nsv585286 CNV loss 21841781
nsv828673 CNV gain 20364138
nsv833899 CNV loss 17160897

Variation tolerance for CDC25B Gene

Residual Variation Intolerance Score: 53.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.07; 37.98% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for CDC25B Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
CDC25B

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CDC25B Gene

Disorders for CDC25B Gene

MalaCards: The human disease database

(3) MalaCards diseases for CDC25B Gene - From: DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
breast cancer
  • breast lobular carcinoma
esophageal cancer
  • esophageal cancer, somatic
primary autosomal recessive microcephaly
  • mcph
- elite association - COSMIC cancer census association via MalaCards

Additional Disease Information for CDC25B

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
Tumor Gene Database
(TGDB)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
genes like me logo Genes that share disorders with CDC25B: view

No data available for UniProtKB/Swiss-Prot and Genatlas for CDC25B Gene

Publications for CDC25B Gene

  1. Specific activation of cdc25 tyrosine phosphatases by B-type cyclins: evidence for multiple roles of mitotic cyclins. (PMID: 1836978) Galaktionov K … Beach D (Cell 1991) 2 3 4 23
  2. Rho GTPases regulate PRK2/PKN2 to control entry into mitosis and exit from cytokinesis. (PMID: 17332740) Schmidt A … Hall A (The EMBO journal 2007) 3 4 23
  3. CDC25B phosphorylated by pEg3 localizes to the centrosome and the spindle poles at mitosis. (PMID: 15908796) Mirey G … Ducommun B (Cell cycle (Georgetown, Tex.) 2005) 3 4 23
  4. Phosphorylation of CDC25B by Aurora-A at the centrosome contributes to the G2-M transition. (PMID: 15128871) Dutertre S … Ducommun B (Journal of cell science 2004) 3 4 23
  5. Human pEg3 kinase associates with and phosphorylates CDC25B phosphatase: a potential role for pEg3 in cell cycle regulation. (PMID: 12400006) Davezac N … Tassan JP (Oncogene 2002) 3 4 23

Products for CDC25B Gene

Sources for CDC25B Gene