Aliases for BRD4 Gene
Aliases for BRD4 Gene
External Ids for BRD4 Gene
- HGNC: 13575
- Entrez Gene: 23476
- Ensembl: ENSG00000141867
- OMIM: 608749
- UniProtKB: O60885
Previous GeneCards Identifiers for BRD4 Gene
- GC19M015727
- GC19M015193
- GC19M015209
- GC19M015348
- GC19M014916
Summaries for BRD4 Gene
-
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]
GeneCards Summary for BRD4 Gene
BRD4 (Bromodomain Containing 4) is a Protein Coding gene. Diseases associated with BRD4 include Nut Midline Carcinoma and Spinal Canal Intradural Extramedullary Neoplasm. Among its related pathways are Chromatin Regulation / Acetylation. Gene Ontology (GO) annotations related to this gene include chromatin binding and lysine-acetylated histone binding. An important paralog of this gene is BRD3.
UniProtKB/Swiss-Prot for BRD4 Gene
-
Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of Ser-2 of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of Ser-2 of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters.
-
Isoform B: Acts as a chromatin insulator in the DNA damage response pathway. Inhibits DNA damage response signaling by recruiting the condensin-2 complex to acetylated histones, leading to chromatin structure remodeling, insulating the region from DNA damage response by limiting spreading of histone H2AFX/H2A.x phosphorylation.
-
Bromodomains (BRDs) are epigenetic reader domains that selectively recognize acetylated lysine residues on the tails of histone proteins, and are the only known protein modules that can target acetylated lysine residues.
Additional gene information for BRD4 Gene
- Monarch Initiative
- Search for BRD4 at DataMed
- Search for BRD4 at HumanCyc
No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for BRD4 Gene
Genomics for BRD4 Gene
GeneHancer (GH) Regulatory Elements for BRD4 Gene
Promoters and enhancers for BRD4 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association
Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)
GeneHancers around BRD4 on UCSC Golden Path with GeneCards custom track
- Top Transcription factor binding sites by QIAGEN in the BRD4 gene promoter:
-
- p53
- AP-1
- NF-1
- FOXO3b
- FOXO3a
- FOXO3
- RFX1
Regulatory Element Products
Genomic Locations for BRD4 Gene
Genomic Locations for BRD4 Gene
- chr19:15,235,519-15,332,545
- (GRCh38/hg38)
- Size:
- 97,027 bases
- Orientation:
- Minus strand
- chr19:15,347,647-15,443,356
- (GRCh37/hg19)
- Size:
- 95,710 bases
- Orientation:
- Minus strand
Genomic View for BRD4 Gene
Genes around BRD4 on UCSC Golden Path with GeneCards custom track
- Cytogenetic band:
-
- 19p13.12 by Entrez Gene
- 19p13.12 by HGNC
- 19p13.12 by Ensembl
BRD4 Gene in genomic location: bands according to
Ensembl, locations according to GeneLoc
(and/or Entrez Gene and/or Ensembl if different)
Genomic Neighborhood
• Exon Structure
• Gene Density
RefSeq DNA sequence for BRD4 Gene
Proteins for BRD4 Gene
-
Protein details for BRD4 Gene (UniProtKB/Swiss-Prot)
- Protein Symbol:
- O60885-BRD4_HUMAN
- Recommended name:
- Bromodomain-containing protein 4
- Protein Accession:
- O60885
- O60433
- Q4G0X8
- Q86YS8
- Q96PD3
Protein attributes for BRD4 Gene
- Size:
- 1362 amino acids
- Molecular mass:
- 152219 Da
- Quaternary structure:
-
- Interacts with p53/TP53; the interaction is direct. Interacts (via CTD region) with CDK9 and CCNT1, acting as an associated component of P-TEFb complex. Interacts with RELA (when acetylated at Lys-310). Interacts (via NET domain) with WHSC1L1, JMJD6, CHD4, GLTSCR1 and ATAD5. Isoform B: interacts with NCAPD3 and SMC2. Interacts with bovine papillomavirus type 1 regulatory protein E2 and human herpes virus-8 (HHV-8) protein LANA. These interactions may serve for the tethering of viral genomes to host mitotic chromosomes allowing successful partitioning of the viral genome during cell division. Interacts with Epstein-Barr virus (EBV) protein EBNA1; this interaction facilitates transcriptional activation by EBNA1.
- Miscellaneous:
-
- Some specific inhibitors of BRD4 that prevent binding to acetylated histones by binding Asn-140 and Asn-433 are promising therapeutic molecules for the treatment of leukemias. JQ1, a thieno-triazolo-1,4-diazepine derivative, and I-BET, a benzodiazepine derivative, have been tested on tumors with success (PubMed:20871596, PubMed:21068722, PubMed:21964340). Treatment with GSK1210151A (I-BET151, a I-BET derivative) has strong effets on mixed lineage leukemia and promotes myeloid differentiation and leukemia stem-cell depletion (PubMed:21964340).
- SequenceCaution:
-
- Sequence=AAC27978.1; Type=Erroneous initiation; Evidence={ECO:0000305};
Three dimensional structures from OCA and Proteopedia for BRD4 Gene
Protein Expression for BRD4 Gene
Post-translational modifications for BRD4 Gene
- Phosphorylation by CK2 disrupt the intramolecular binding between the bromo domain 2 and the NPS region and promotes binding between the NPS and the BID regions, leading to activate the protein and promote binding to acetylated histones. In absence of phosphorylation, BRD4 does not localize to p53/TP53 target gene promoters, phosphorylation promoting recruitment to p53/TP53 target promoters.
- Ubiquitination at posLast=9999
- Modification sites at PhosphoSitePlus
Other Protein References for BRD4 Gene
- ENSEMBL proteins:
- REFSEQ proteins:
Antibody Products
- Novus Biologicals Antibodies for BRD4
-
Abcam antibodies for BRD4
- Invitrogen Antibodies for BRD4
- GeneTex BRD4 Antibody for BRD4
-
Santa Cruz Biotechnology (SCBT) Antibodies for BRD4
Protein Products
-
OriGene Purified Proteins for BRD4
- Search Origene for MassSpec and Protein Over-expression Lysates for BRD4
- Origene Custom Protein Services for BRD4
- Search GeneTex for Proteins for BRD4
-
Abcam proteins for BRD4
Assay Products
No data available for DME Specific Peptides for BRD4 Gene
Domains & Families for BRD4 Gene
Gene Families for BRD4 Gene
- HGNC:
- IUPHAR :
- Human Protein Atlas (HPA):
-
- Cancer-related genes
- Disease related genes
- Predicted intracellular proteins
Protein Domains for BRD4 Gene
- Blocks:
- InterPro:
- ProtoNet:
Suggested Antigen Peptide Sequences for BRD4 Gene
- GenScript: Design optimal peptide antigens:
Graphical View of Domain Structure for InterPro Entry
O60885UniProtKB/Swiss-Prot:
BRD4_HUMAN :- The NET domain mediates interaction with a number of chromatin proteins involved in transcription regulation (WHSC1L1, JMJD6, CHD4, GLTSCR1 and ATAD5).
- Domain:
-
- The NET domain mediates interaction with a number of chromatin proteins involved in transcription regulation (WHSC1L1, JMJD6, CHD4, GLTSCR1 and ATAD5).
- The C-terminal (CTD) region mediates interaction and recruitment of CDK9 and CCNT1 subunits of the P-TEFb complex (PubMed:16109376, PubMed:16109377). It is also required for maintenance of higher-order chromatin structure (PubMed:22334664).
- The 2 bromo domains mediate specific binding to acetylated histones via Asn-140 and Asn-433, respectively (PubMed:20871596). The exact combination of modified histone tails required to recruit BRD4 to target genes is still unclear. The first bromo domain has high affinity for acetylated histone H4 tail, whereas the second bromo domain recognizes multiply acetylated marks in histone H3 (PubMed:22464331). A number of specific inhibitors bind competitively to acetyl-lysine-binding residues Asn-140 and Asn-433, promoting removal from acetylated histones. Many of these inhibitors are benzodiazepine derivatives (PubMed:22137933, PubMed:22136404, PubMed:23517011, PubMed:23530754).
Function for BRD4 Gene
Molecular function for BRD4 Gene
- UniProtKB/Swiss-Prot Function:
- Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of Ser-2 of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of Ser-2 of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters.
- UniProtKB/Swiss-Prot Function:
- Isoform B: Acts as a chromatin insulator in the DNA damage response pathway. Inhibits DNA damage response signaling by recruiting the condensin-2 complex to acetylated histones, leading to chromatin structure remodeling, insulating the region from DNA damage response by limiting spreading of histone H2AFX/H2A.x phosphorylation.
Phenotypes From GWAS Catalog for BRD4 Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000993 | RNA polymerase II core binding | IDA | 22509028 |
| GO:0002039 | p53 binding | IDA | 23317504 |
| GO:0003682 | chromatin binding | IDA | 20871596 |
| GO:0005515 | protein binding | IEA,IPI | 15109495 |
| GO:0070577 | lysine-acetylated histone binding | IDA | 20871596 |
Phenotypes for BRD4 Gene
- GenomeRNAi human phenotypes for BRD4:
-
- Increased HPV18 LCR reporter activity
- Decreased shRNA abundance (Z-score < -2)
- Decreased substrate adherent cell growth
- Dynamic nuclei (hole, folded or small irregular)
- Increased shRNA abundance (Z-score > 2)
- Decreased focal adhesion (FA) area, decreased FA length, decreased FA mean intensity, increased number of small and round FAs, increased FA abundance
- Increased cell death HMECs cells
- Upregulation of Wnt/beta-catenin pathway after WNT3A stimulation
- Increased cilium length after serum starvation
- Synthetic lethal with c-Myc after tamoxifen stimulation
- Decreased viability of wild-type and TP53 knockout cells, decreased TP53 protein expression ratio (wild-type / TP53 knockout cells)
- Reduced mammosphere formation
- Decreased ionizing radiation sensitivity
- Increased Nanog expression
- Decreased viability
- no effect
- Decreased human cytomegalovirus (HCMV) strain AD169 replication
- Increased vaccinia virus (VACV) infection
- Resistant to vaccinia virus (VACV-A4L) infection
- Mildly decreased CFP-tsO45G cell surface transport
- shRNA abundance <= 50%
- Increased viability
- Synthetic lethal with MLN4924 (a NAE inhibitor)
- Increased viability with MLN4924 (a NAE inhibitor)
- Transferrin accumulation in the perinuclear area
- Decreased vesicular stomatitis virus (VSV) infection
Animal Model Products
-
Taconic Biosciences Mouse Models for BRD4
- Cyagen custom Knockout/knockin (KOKI) mouse models for BRD4
-
CRISPR Products
-
OriGene CRISPR knockouts for BRD4
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for BRD4
- genomics-online: gRNA clones - Search results for 102 available BRD4 gene related products
- Overview of 102 available BRD4 gene related products
- Applied Biological Materials CRISPR for BRD4
-
Vectors and viruses for KO, Activation, Repression, and more
-
Santa Cruz Biotechnology (SCBT) CRISPR for BRD4
- GenScript: Design CRISPR guide RNA sequences for BRD4
miRNA for BRD4 Gene
- miRTarBase miRNAs that target BRD4
-
- hsa-mir-26b-5p (MIRT029120)
- hsa-mir-2110 (MIRT035757)
- hsa-mir-877-3p (MIRT036894)
- hsa-mir-455-3p (MIRT037828)
- hsa-mir-484 (MIRT042219)
- hsa-mir-320a (MIRT044471)
- hsa-mir-544a (MIRT441106)
- hsa-mir-3680-3p (MIRT613269)
- hsa-mir-557 (MIRT613270)
- hsa-mir-507 (MIRT613271)
- hsa-mir-450b-5p (MIRT613272)
- hsa-mir-5006-3p (MIRT613273)
- hsa-mir-4755-5p (MIRT613274)
- hsa-mir-623 (MIRT613275)
- hsa-mir-6731-3p (MIRT613276)
- hsa-mir-6832-3p (MIRT613277)
- hsa-mir-3938 (MIRT613278)
- hsa-mir-211-5p (MIRT613279)
- hsa-mir-204-5p (MIRT613280)
- hsa-mir-3613-3p (MIRT615093)
- hsa-mir-548z (MIRT615094)
- hsa-mir-548h-3p (MIRT615095)
- hsa-mir-548d-3p (MIRT615096)
- hsa-mir-548bb-3p (MIRT615097)
- hsa-mir-548ac (MIRT615098)
- hsa-mir-380-3p (MIRT636682)
- hsa-mir-411-3p (MIRT636683)
- hsa-mir-379-3p (MIRT636684)
- hsa-mir-4495 (MIRT636685)
- hsa-mir-1228-3p (MIRT660145)
- hsa-mir-8485 (MIRT660146)
- hsa-mir-362-3p (MIRT660147)
- hsa-mir-329-3p (MIRT660148)
- hsa-mir-1306-5p (MIRT660149)
miRNA Products
Inhibitory RNA Products
- Origene shrna, sirna, and RNAi products in human, mouse, rat for BRD4
- Browse OriGene Inhibitory RNA Products For BRD4
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for BRD4
- genomics-online: shRNA clones - Search results for 102 available BRD4 gene related products
- Overview of 102 available BRD4 gene related products
Clone Products
- Sino Biological Human cDNA Clone for BRD4
- VectorBuilder custom plasmid, inducible vectors for BRD4
- VectorBuilder custom lentivirus, adenovirus, AAV vector/virus packaging for BRD4
-
VectorBuilder Other custom vectors
- Mammalian expression: PiggyBac
- Mammalian Tet-on expression: plasmid
- Mammalian conditional (Cre-Lox): plasmid and PiggyBac
- Mammalian shRNA knockdown: lentiviral, adenoviral, AAV, and PiggyBac
- CRISPR: plasmid gRNA, lentiviral gRNA, and donor plasmid
- Bacterial expression: pET, pBAD, and pCS
- Yeast expression
- Addgene plasmids for BRD4
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for BRD4
- Applied Biological Materials Clones for BRD4
-
Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more
Cell Line Products
-
Horizon Cell Lines for BRD4
No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for BRD4 Gene
Localization for BRD4 Gene
Subcellular locations from UniProtKB/Swiss-Prot for BRD4 Gene
- Nucleus. Chromosome. Note=Associates with acetylated chromatin. Released from chromatin upon deacetylation of histones that can be triggered by different signals such as activation of the JNK pathway or nocodazole treatment.
| Compartment | Confidence |
|---|---|
| nucleus | 5 |
| cytosol | 5 |
| extracellular | 1 |
- Nucleoplasm (4)
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000794 | condensed nuclear chromosome | IDA | 10938129 |
| GO:0005634 | nucleus | IEA,IDA | 20871596 |
| GO:0005654 | nucleoplasm | IDA | -- |
| GO:0005694 | chromosome | IEA | -- |
| GO:0005829 | cytosol | IDA | -- |
Pathways & Interactions for BRD4 Gene
| SuperPathway | Contained pathways | ||
|---|---|---|---|
| 1 | Chromatin Regulation / Acetylation | ||
Pathways by source for BRD4 Gene
1 GeneTex pathway for BRD4 Gene
1 Cell Signaling Technology pathway for BRD4 Gene
Interacting Proteins for BRD4 Gene
Selected Interacting proteins:
O60885-BRD4_HUMAN
ENSP00000263377
for BRD4 Gene via
UniProtKB
MINT
STRING
IID
GPS-Prot Interaction Network for BRD4
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000083 | regulation of transcription involved in G1/S transition of mitotic cell cycle | IMP | 18039861 |
| GO:0006325 | chromatin organization | IEA | -- |
| GO:0006351 | transcription, DNA-templated | IEA | -- |
| GO:0006355 | regulation of transcription, DNA-templated | IEA | -- |
| GO:0006974 | cellular response to DNA damage stimulus | IEA | -- |
Drugs & Compounds for BRD4 Gene
| Name | Synonyms | Role | CAS Number | PubChem IDs | PubMed IDs | |
|---|---|---|---|---|---|---|
| I-BET 151 dihydrochloride |
|
1883545-47-8 |
|
|
||
| PFI 1 |
|
1403764-72-6 |
|
|
(5) Tocris Compounds for BRD4 Gene
| Compound | Action | Cas Number |
|---|---|---|
| (+)-JQ1 | Potent, selective BET bromodomain inhibitor; cell permeable | 1268524-70-4 |
| Bromosporine | Broad spectrum bromodomain inhibitor | |
| I-BET 151 dihydrochloride | BET bromodomain inhibitor; also promotes differentiation of hiPSCs into megakaryocytes | 1883545-47-8 |
| Ischemin sodium salt | CBP inhibitor; cell permeable | |
| PFI 1 | BET bromodomain inhibitor | 1403764-72-6 |
Drug Products
Transcripts for BRD4 Gene
mRNA/cDNA for BRD4 Gene
- (5) REFSEQ mRNAs :
- (12) Additional mRNA sequences :
- (502) Selected AceView cDNA sequences:
- (12) Ensembl transcripts including schematic representations, and UCSC links where relevant :
-
- ENST00000263377 7169 bps (uc002nar.4)
- ENST00000594066 587 bps (uc060usj.1)
- ENST00000371835 4624 bps (uc002nas.4)
- ENST00000360016 3240 bps (uc002nat.4)
- ENST00000595926 582 bps (uc060usk.1)
Unigene Clusters for BRD4 Gene
CRISPR Products
-
OriGene CRISPR knockouts for BRD4
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for BRD4
- genomics-online: gRNA clones - Search results for 102 available BRD4 gene related products
- Overview of 102 available BRD4 gene related products
- Applied Biological Materials CRISPR for BRD4
-
Vectors and viruses for KO, Activation, Repression, and more
-
Santa Cruz Biotechnology (SCBT) CRISPR for BRD4
- GenScript: Design CRISPR guide RNA sequences for BRD4
miRNA Products
Inhibitory RNA Products
- Origene shrna, sirna, and RNAi products in human, mouse, rat for BRD4
- Browse OriGene Inhibitory RNA Products For BRD4
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for BRD4
- genomics-online: shRNA clones - Search results for 102 available BRD4 gene related products
- Overview of 102 available BRD4 gene related products
Clone Products
- Sino Biological Human cDNA Clone for BRD4
- VectorBuilder custom plasmid, inducible vectors for BRD4
- VectorBuilder custom lentivirus, adenovirus, AAV vector/virus packaging for BRD4
-
VectorBuilder Other custom vectors
- Mammalian expression: PiggyBac
- Mammalian Tet-on expression: plasmid
- Mammalian conditional (Cre-Lox): plasmid and PiggyBac
- Mammalian shRNA knockdown: lentiviral, adenoviral, AAV, and PiggyBac
- CRISPR: plasmid gRNA, lentiviral gRNA, and donor plasmid
- Bacterial expression: pET, pBAD, and pCS
- Yeast expression
- Addgene plasmids for BRD4
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for BRD4
- Applied Biological Materials Clones for BRD4
-
Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more
| ExUns: | 1 | ^ | 2 | ^ | 3 | ^ | 4 | ^ | 5 | ^ | 6 | ^ | 7 | ^ | 8 | ^ | 9 | ^ | 10 | ^ | 11 | ^ | 12 | ^ | 13 | ^ | 14 | ^ | 15 | ^ | 16a | · | 16b | ^ | 17a | · | 17b | ^ | 18 | ^ | 19 | ^ | 20 | ^ | 21 | ^ | 22 | ^ | 23 | ^ | 24a | · |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
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| SP12: | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| SP13: |
| ExUns: | 24b | ^ | 25 | ^ | 26 | ^ | 27 | ^ | 28 | ^ | 29 | ^ | 30 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SP1: | |||||||||||||
| SP2: | |||||||||||||
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| SP12: | |||||||||||||
| SP13: |
Expression for BRD4 Gene
This gene is overexpressed in Adrenal (13.6), Peripheral blood mononuclear cells (10.0), and Lymph node (8.9).
Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for BRD4 Gene
- Elite partner
NURSA nuclear receptor signaling pathways regulating expression of BRD4 Gene:
BRD4SOURCE GeneReport for Unigene cluster for BRD4 Gene:
Hs.187763mRNA Expression by UniProt/SwissProt for BRD4 Gene:
O60885-BRD4_HUMAN
Tissue specificity:
Ubiquitously expressed.
Evidence on tissue expression from TISSUES for BRD4 Gene
- Liver(4.5)
- Nervous system(4)
- Blood(3)
- Lung(2.9)
- Muscle(2.9)
- Intestine(2.6)
- Kidney(2.6)
- Heart(2.4)
- Thyroid gland(2)
- Urine(2)
Primer Products
-
OriGene qPCR primer pairs for BRD4
Gene Expression Assay Products
No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for BRD4 Gene
Orthologs for BRD4 Gene
This gene was present in the common ancestor of animals.
| Organism | Taxonomy | Gene | Similarity | Type | Details |
|---|---|---|---|---|---|
| dog (Canis familiaris) |
Mammalia | BRD4 34 |
|
OneToOne | |
| chimpanzee (Pan troglodytes) |
Mammalia | BRD4 34 33 |
|
OneToOne | |
| cow (Bos Taurus) |
Mammalia | BRD4 34 |
|
OneToOne | |
| mouse (Mus musculus) |
Mammalia | Brd4 34 |
|
OneToOne | |
| platypus (Ornithorhynchus anatinus) |
Mammalia | BRD4 34 |
|
OneToOne | |
| oppossum (Monodelphis domestica) |
Mammalia | BRD4 34 |
|
OneToOne | |
| chicken (Gallus gallus) |
Aves | BRD4 34 |
|
OneToOne | |
| LOC100859056 33 |
|
||||
| lizard (Anolis carolinensis) |
Reptilia | BRD4 34 |
|
OneToOne | |
| zebrafish (Danio rerio) |
Actinopterygii | brd4 34 |
|
OneToOne | |
| fruit fly (Drosophila melanogaster) |
Insecta | fs(1)h 34 35 |
|
OneToMany |
- Species where no ortholog for BRD4 was found in the sources mined by GeneCards:
-
- A. gosspyii yeast (Ashbya gossypii)
- Actinobacteria (Mycobacterium tuberculosis)
- African clawed frog (Xenopus laevis)
- African malaria mosquito (Anopheles gambiae)
- Alicante grape (Vitis vinifera)
- alpha proteobacteria (Wolbachia pipientis)
- amoeba (Dictyostelium discoideum)
- Archea (Pyrococcus horikoshii)
- baker's yeast (Saccharomyces cerevisiae)
- barley (Hordeum vulgare)
- beta proteobacteria (Neisseria meningitidis)
- bread mold (Neurospora crassa)
- Chromalveolata (Phytophthora infestans)
- common water flea (Daphnia pulex)
- corn (Zea mays)
- E. coli (Escherichia coli)
- filamentous fungi (Aspergillus nidulans)
- Firmicute bacteria (Streptococcus pneumoniae)
- fission yeast (Schizosaccharomyces pombe)
- green algae (Chlamydomonas reinhardtii)
- honey bee (Apis mellifera)
- K. lactis yeast (Kluyveromyces lactis)
- loblloly pine (Pinus taeda)
- malaria parasite (Plasmodium falciparum)
- medicago trunc (Medicago Truncatula)
- moss (Physcomitrella patens)
- orangutan (Pongo pygmaeus)
- pig (Sus scrofa)
- rainbow trout (Oncorhynchus mykiss)
- rat (Rattus norvegicus)
- rice (Oryza sativa)
- rice blast fungus (Magnaporthe grisea)
- schistosome parasite (Schistosoma mansoni)
- sea anemone (Nematostella vectensis)
- sea squirt (Ciona intestinalis)
- sea squirt (Ciona savignyi)
- sea urchin (Strongylocentrotus purpuratus)
- sorghum (Sorghum bicolor)
- soybean (Glycine max)
- stem rust fungus (Puccinia graminis)
- sugarcane (Saccharum officinarum)
- thale cress (Arabidopsis thaliana)
- tomato (Lycopersicon esculentum)
- toxoplasmosis (Toxoplasma gondii)
- Trichoplax (Trichoplax adhaerens)
- tropical clawed frog (Silurana tropicalis)
- wheat (Triticum aestivum)
- worm (Caenorhabditis elegans)
Evolution for BRD4 Gene
- ENSEMBL:
- Gene Tree for BRD4 (if available)
- TreeFam:
- Gene Tree for BRD4 (if available)
- Aminode:
- Evolutionary constrained regions (ECRs) for BRD4: view image
Paralogs for BRD4 Gene
(8) SIMAP similar genes for BRD4 Gene using alignment to 8 proteins:
- BRD4_HUMAN
- M0QYW0_HUMAN
- M0QZD9_HUMAN
- M0R0H4_HUMAN
- Q05BM2_HUMAN
- Q5BJ26_HUMAN
- Q6NXE4_HUMAN
- Q6PIS5_HUMAN
Variants for BRD4 Gene
| SNP ID | Clin | Chr 19 pos | Variation | AA Info | Type |
|---|---|---|---|---|---|
| rs1000002445 | -- | 15,300,138(-) | T/C | genic_upstream_transcript_variant, intron_variant | |
| rs1000017091 | -- | 15,334,136(-) | G/A | upstream_transcript_variant | |
| rs1000017825 | -- | 15,257,658(-) | C/A | intron_variant | |
| rs1000040567 | -- | 15,240,891(-) | G/A | genic_downstream_transcript_variant, intron_variant | |
| rs1000106340 | -- | 15,330,540(-) | T/A | genic_upstream_transcript_variant, intron_variant |
| Variant ID | Type | Subtype | PubMed ID |
|---|---|---|---|
| dgv3442n100 | CNV | loss | 25217958 |
| esv3382180 | CNV | insertion | 20981092 |
| esv34154 | CNV | loss | 18971310 |
| nsv1078911 | CNV | insertion | 25765185 |
| nsv474976 | CNV | novel sequence insertion | 20440878 |
| nsv578717 | CNV | loss | 21841781 |
| nsv953983 | CNV | deletion | 24416366 |
Residual Variation Intolerance Score:
1.79% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score:
2.96;
49.43% of all genes are more intolerant (likely to be disease-causing)
Additional Variant Information for BRD4 Gene
SNP Genotyping and Copy Number Assay Products
No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for BRD4 Gene
Disorders for BRD4 Gene
| Disorder | Aliases | PubMed IDs |
|---|---|---|
| nut midline carcinoma |
|
|
| spinal canal intradural extramedullary neoplasm |
|
|
| diffuse lipomatosis |
|
|
| hyperglobulinemic purpura |
|
|
| functional colonic disease |
|
|
Search BRD4 in MalaCards
View complete list of genes associated with diseases
UniProtKB/Swiss-Prot
BRD4_HUMAN- Note=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein. {ECO:0000269 PubMed:11733348, ECO:0000269 PubMed:12543779}.
Additional Disease Information for BRD4
No data available for Genatlas for BRD4 Gene
Publications for BRD4 Gene
- BRD4-NUT fusion oncogene: a novel mechanism in aggressive carcinoma. (PMID: 12543779) French CA … Fletcher JA (Cancer research 2003) 3 4 22 58
- BRD4 bromodomain gene rearrangement in aggressive carcinoma with translocation t(15;19). (PMID: 11733348) French CA … Fletcher JA (The American journal of pathology 2001) 3 4 22 58
- A bromodomain protein, MCAP, associates with mitotic chromosomes and affects G(2)-to-M transition. (PMID: 10938129) Dey A … Ozato K (Molecular and cellular biology 2000) 2 3 22 58
- The bromodomain protein Brd4 insulates chromatin from DNA damage signalling. (PMID: 23728299) Floyd SR … Yaffe MB (Nature 2013) 3 4 58
- Phospho switch triggers Brd4 chromatin binding and activator recruitment for gene-specific targeting. (PMID: 23317504) Wu SY … Chiang CM (Molecular cell 2013) 3 4 58
Products for BRD4 Gene
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- Origene shrna, sirna, and RNAi products in human, mouse, rat for BRD4
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- Custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
- Browse OriGene miRNA Products For BRD4
- GenScript: Next-day shipping of latest version cDNA ORF clones for BRD4 in any vector
- GenScript Custom Purified and Recombinant Proteins Services for BRD4
- GenScript Custom Assay Services for BRD4
- GenScript Custom overexpressing Cell Line Services for BRD4
- GenScript: Design CRISPR guide RNA sequences for BRD4
- Design optimal peptide antigens
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- Gene Synthesis: Any Gene in Any Vector
- Vector-based siRNA and miRNA, Ready for Transfection
- Gene Mutant Library, Variants up to 10^11
- Plasmid Preparation
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- Sino Biological Human cDNA Clone for BRD4
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- High-throughput Antibody Production Service
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- Epitope Tag Antibodies & Reporter Protein Antibodies
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- Novus Biologicals Antibodies for BRD4
- Novus Biologicals proteins and lysates for BRD4
- Novus Biologicals
- Novus Biologicals Tissue Microarrays
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- Taconic Biosciences Mouse Models for BRD4
- Find your next knockout model in the Taconic Knockout Repository
- Genetically Engineered Models Available Immediately
- Humanized Mice
- Invitrogen Antibodies for BRD4
- Cyagen custom Knockout/knockin (KOKI) mouse models for BRD4
- VectorBuilder custom plasmid, inducible vectors for BRD4
- VectorBuilder custom lentivirus, adenovirus, AAV vector/virus packaging for BRD4
- VectorBuilder Other custom vectors
- Mammalian expression: PiggyBac
- Mammalian Tet-on expression: plasmid
- Mammalian conditional (Cre-Lox): plasmid and PiggyBac
- Mammalian shRNA knockdown: lentiviral, adenoviral, AAV, and PiggyBac
- CRISPR: plasmid gRNA, lentiviral gRNA, and donor plasmid
- Bacterial expression: pET, pBAD, and pCS
- Yeast expression
- Addgene plasmids for BRD4
- antibodies-online: Search results for 103 available BRD4 Antibodies ranked by validation data
- Compare Top BRD4 Antibodies
- antibodies-online: Search results for 5 available BRD4 Elisa Kits ranked by validation data
- Compare Top BRD4 Elisa Kits
- Recommended
- antibodies-online: Search results for 8 available BRD4 Proteins ranked by validation data
- Compare Top BRD4 Proteins
- GeneTex BRD4 Antibody for BRD4
- Search GeneTex for Proteins for BRD4
- Santa Cruz Biotechnology (SCBT) Antibodies for BRD4
- Search Santa Cruz Biotechnology (SCBT) for BRD4 siRNA/shRNA
- Santa Cruz Biotechnology (SCBT) CRISPR for BRD4
- Horizon Cell Lines for BRD4
- genomics-online: cdna clones - Search results for 102 available BRD4 gene related products
- Overview of 102 available BRD4 gene related products
- orf clones - Search results for 102 available BRD4 gene related products
- Overview of 102 available BRD4 gene related products
- genomics-online: gRNA clones - Search results for 102 available BRD4 gene related products
- Overview of 102 available BRD4 gene related products
- genomics-online: primer clones - Search results for 102 available BRD4 gene related products
- Overview of 102 available BRD4 gene related products
- genomics-online: shRNA clones - Search results for 102 available BRD4 gene related products
- Overview of 102 available BRD4 gene related products
- Browse GeneCopoeia Cell Lines
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for BRD4
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for BRD4
- GeneCopoeia miRNA 3'UTR Target clone products for BRD4
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for BRD4
Sources for BRD4 Gene
- (1) GeneCards
- (2) HGNC
- (3) EntrezGene
- (4) Swiss-Prot
- (5) Ensembl
- (6) OMIM
- (7) GeneLoc
- (8) Gene Wiki
- (9) UCSC
- (10) PhosphoSitePlus
- (11) GO
- (12) TrEMBL
- (13) InterPro
- (14) ProtoNet
- (15) Blocks
- (16) MGI
- (17) IUBMB
- (18) KEGG
- (19) MINT
- (20) STRING
- (21) IntAct
- (22) Novoseek
- (23) PharmGKB
- (24) DrugBank
- (25) HMDB
- (26) UniGene
- (27) AceView
- (28) ASD
- (29) ECgene
- (30) GeneAnnot
- (31) CGAP SAGE
- (32) SOURCE
- (33) HomoloGene
- (34) PanEnsembl
- (35) euGenes
- (36) SGD
- (37) FlyBase
- (38) WormBase
- (39) Pseudogene
- (40) DGV
- (41) dbSNP
- (42) GenAtlas
- (43) HGMD
- (44) GAD
- (45) BGMUT
- (46) HuGE
- (47) Atlas
- (48) Cell Signaling Technology
- (49) GenBank
- (50) H-invDB
- (51) HORDE
- (52) HUGE
- (53) IMGT
- (54) Leiden
- (55) miRBase
- (56) DME
- (57) OriGene
- (58) PubMed
- (59) R&D Systems
- (60) TGDB
- (61) Tocris
- (62) Abcam
- (63) Novus Biologicals
- (64) ProSpec
- (65) Sino Biological
- (66) GenScript
- (67) Qiagen
- (68) Cloud-Clone Corp.
- (69) OCA
- (70) Proteopedia
- (71) MOPED
- (72) neXtProt
- (73) Reactome
- (74) GeneGo (Thomson Reuters)
- (75) fRNAdb
- (76) DISEASES
- (77) SIMAP
- (78) GenomeRNAi
- (79) LifeMap
- (80) miRTarBase
- (81) MalaCards
- (82) Invitrogen
- (83) BitterDB
- (84) Vector BioLabs
- (85) ESI-BIO
- (86) RefSeq
- (87) BioSystems
- (88) MaxQB
- (89) IUPHAR
- (90) BioGPS
- (91) Illumina
- (92) COMPARTMENTS
- (93) HOMER
- (94) PaxDb
- (95) ApexBio
- (96) Addgene
- (97) antibodies-online
- (98) CYP
- (99) NONCODE
- (100) SwitchGear Genomics
- (101) TreeFam
- (102) PathCards
- (103) GeneReviews
- (104) GeneTex
- (105) Taconic Biosciences
- (106) GTEx
- (107) ProteomicsDB
- (108) SCBT
- (109) DGIdb
- (110) ClinicalTrials
- (111) FDA Approved Drugs
- (112) RVIS
- (113) SIGNOR
- (114) diseasecard
- (115) NIH Rare Diseases
- (116) Orphanet
- (117) UMLS
- (118) GTR
- (119) Disease Ontology
- (120) Genetics Home Reference
- (121) MeSH
- (122) MedlinePlus
- (123) CDC
- (124) NINDS
- (125) NCBI Bookshelf
- (126) ClinVar
- (127) Gene Damage Index
- (128) ViGene Biosciences
- (129) HPO
- (130) UDN
- (131) VISTA
- (132) FANTOM5
- (133) ENCODE
- (134) ProSci
- (135) Horizon
- (136) NURSA
- (137) IID
- (138) Cyagen
- (139) VectorBuilder
- (140) SNPedia
- (141) BRCA Exchange
- (142) St John's Lab
- (143) CIViC
- (144) ProteoGenix
- (145) dbSUPER
- (146) TISSUES
- (147) Gene ORGANizer
- (148) abm
- (149) CrownBio
- (150) Human Protein Atlas
- (151) GWAS Catalog
- (152) Monarch Initiative
- (153) DataMed
- (154) HumanCyc
- (155) genomics-online
- (156) UCNEbase
- (157) EPDnew
- (158) Applied Biosystems
- (159) GlyConnect
- (160) Aminode
- (161) GeneCopoeia
- (162) GPS-Prot
- (163) RNACentral
- (164) LncRNADisease
- (165) LOVD
- (166) miR2Disease
- (167) DiseaseEnhancer
- (168) Boster Bio
- (169) Biorbyt