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Aliases for BRAF Gene

Aliases for BRAF Gene

  • B-Raf Proto-Oncogene, Serine/Threonine Kinase 2 3 5
  • V-Raf Murine Sarcoma Viral Oncogene Homolog B1 3 4
  • V-Raf Murine Sarcoma Viral Oncogene Homolog B 2 3
  • Proto-Oncogene B-Raf 3 4
  • BRAF1 3 4
  • RAFB1 3 4
  • B-Raf Proto-Oncogene Serine/Threonine-Protein Kinase (P94) 3
  • Murine Sarcoma Viral (V-Raf) Oncogene Homolog B1 3
  • Serine/Threonine-Protein Kinase B-Raf 3
  • B-Raf Serine/Threonine-Protein 3
  • 94 KDa B-Raf Protein 3
  • EC 2.7.11.1 4
  • B-RAF1 3
  • B-Raf 3
  • NS7 3
  • P94 4

External Ids for BRAF Gene

Previous GeneCards Identifiers for BRAF Gene

  • GC07M138705
  • GC07M139764
  • GC07M139841
  • GC07M139754
  • GC07M139887
  • GC07M140080
  • GC07M140424
  • GC07M134729

Summaries for BRAF Gene

Entrez Gene Summary for BRAF Gene

  • This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]

CIViC summary for BRAF Gene

  • BRAF mutations are found to be recurrent in many cancer types. Of these, the mutation of valine 600 to glutamic acid (V600E) is the most prevalent. V600E has been determined to be an activating mutation, and cells that harbor it, along with other V600 mutations are sensitive to the BRAF inhibitor dabrafenib. It is also common to use MEK inhibition as a substitute for BRAF inhibitors, and the MEK inhibitor trametinib has seen some success in BRAF mutant melanomas. BRAF mutations have also been correlated with poor prognosis in many cancer types, although there is at least one study that questions this conclusion in papillary thyroid cancer.

GeneCards Summary for BRAF Gene

BRAF (B-Raf Proto-Oncogene, Serine/Threonine Kinase) is a Protein Coding gene. Diseases associated with BRAF include Cardiofaciocutaneous Syndrome 1 and Lung Cancer. Among its related pathways are G-protein signaling N-RAS regulation pathway and RET signaling. Gene Ontology (GO) annotations related to this gene include calcium ion binding and transferase activity, transferring phosphorus-containing groups. An important paralog of this gene is RAF1.

UniProtKB/Swiss-Prot for BRAF Gene

  • Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Tocris Summary for BRAF Gene

  • Raf kinases, a family of three serine/threonine kinases, are part of the Ras-MAPK signaling cascade and phosphorylate MEK. Upon growth factor stimulation, Raf-1 (c-Raf) is activated by GTP-bound Ras and recruited to the cell membrane. This activation process is tightly regulated.

Gene Wiki entry for BRAF Gene

PharmGKB "VIP" Summary for BRAF Gene

Additional gene information for BRAF Gene

No data available for fRNAdb sequence ontologies and piRNA Summary for BRAF Gene

Genomics for BRAF Gene

GeneHancer (GH) Regulatory Elements for BRAF Gene

Promoters and enhancers for BRAF Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH07I140923 Promoter/Enhancer 2.3 EPDnew Ensembl ENCODE dbSUPER 550.8 +0.1 141 2.9 HDGF PKNOX1 CLOCK SMAD1 FOXA2 ARNT ARID4B SIN3A DMAP1 IRF4 BRAF LOC105375536 NDUFB2 MRPS33 NDUFB2-AS1 ADCK2 RNU6-85P
GH07I140672 Promoter/Enhancer 2.4 EPDnew FANTOM5 Ensembl ENCODE 10.8 +250.2 250153 4.8 HDGF PKNOX1 ARNT SIN3A FEZF1 DMAP1 IRF4 ZNF766 CBX5 ZNF213 ADCK2 DENND2A BRAF SLC37A3 KDM7A NDUFB2
GH07I141013 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE 10.8 -89.6 -89568 2.6 FEZF1 DMAP1 YY1 E2F8 ZNF143 SP3 NFYC MEF2D GLIS1 NBN MRPS33 SLC37A3 TAS2R6P TAS2R5 TMEM178B BRAF NDUFB2-AS1 NDUFB2 CCT4P1 LOC105375536
GH07I141550 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE 9.7 -626.7 -626662 2 HDGF PKNOX1 SMAD1 MLX ARNT ZFP64 ARID4B SIN3A DMAP1 ZNF2 AGK ENSG00000261570 LOC105375538 TAS2R6P WEE2 TAS2R5 TAS2R4 BRAF ENSG00000270157 WEE2-AS1
GH07I140637 Promoter/Enhancer 1.6 Ensembl ENCODE dbSUPER 10.8 +284.9 284925 5.6 HDGF SIN3A GLI4 ZNF2 GLIS2 ZNF416 KLF7 ATF7 KLF13 YY2 LOC105375535 SLC37A3 NDUFB2 NDUFB2-AS1 BRAF PARP12 ADCK2 TMEM178B GC07P140619 DENND2A
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around BRAF on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the BRAF gene promoter:

Genomic Locations for BRAF Gene

Genomic Locations for BRAF Gene
chr7:140,715,951-140,924,764
(GRCh38/hg38)
Size:
208,814 bases
Orientation:
Minus strand
chr7:140,419,127-140,624,564
(GRCh37/hg19)

Genomic View for BRAF Gene

Genes around BRAF on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
BRAF Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for BRAF Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for BRAF Gene

Proteins for BRAF Gene

  • Protein details for BRAF Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P15056-BRAF_HUMAN
    Recommended name:
    Serine/threonine-protein kinase B-raf
    Protein Accession:
    P15056
    Secondary Accessions:
    • A4D1T4
    • B6HY61
    • B6HY62
    • B6HY63
    • B6HY64
    • B6HY65
    • B6HY66
    • Q13878
    • Q3MIN6
    • Q9UDP8
    • Q9Y6T3

    Protein attributes for BRAF Gene

    Size:
    766 amino acids
    Molecular mass:
    84437 Da
    Cofactor:
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
    Quaternary structure:
    • Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-753. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1 (By similarity). Interacts with DGKH (PubMed:19710016). Interacts with PRMT5 (PubMed:21917714). Interacts with KSR2 (PubMed:21441910). Interacts with AKAP13, MAP2K1 and KSR1. Identified in a complex with AKAP13, MAP2K1 and KSR1 (PubMed:21102438). Interacts with FNIP1 and FNIP2 (PubMed:27353360).
    SequenceCaution:
    • Sequence=AAD43193.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=CAQ43111.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAQ43112.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAQ43113.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAQ43114.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAQ43115.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAQ43116.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for BRAF Gene

neXtProt entry for BRAF Gene

Post-translational modifications for BRAF Gene

  • Methylation at Arg-671 decreases stability and kinase activity.
  • Phosphorylation at Ser-365 by SGK1 inhibits its activity.
  • Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.
  • Ubiquitination at posLast=578578

No data available for DME Specific Peptides for BRAF Gene

Domains & Families for BRAF Gene

Gene Families for BRAF Gene

HGNC:
IUPHAR :
Human Protein Atlas (HPA):
  • Cancer-related genes
  • Disease related genes
  • Enzymes
  • FDA approved drug targets
  • Predicted intracellular proteins

Graphical View of Domain Structure for InterPro Entry

P15056

UniProtKB/Swiss-Prot:

BRAF_HUMAN :
  • Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Family:
  • Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
genes like me logo Genes that share domains with BRAF: view

Function for BRAF Gene

Molecular function for BRAF Gene

GENATLAS Biochemistry:
murine sarcoma 3611 viral (v-raf) oncogene homolog B1 (Rmil),expressed in cerebrum and testis,fused with TIF1A to form the oncoprotein T18
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
Activity is increased by EGF and HGF.
UniProtKB/Swiss-Prot Function:
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Enzyme Numbers (IUBMB) for BRAF Gene

Phenotypes From GWAS Catalog for BRAF Gene

Gene Ontology (GO) - Molecular Function for BRAF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0004672 protein kinase activity IDA,IEA 17563371
GO:0004674 protein serine/threonine kinase activity IDA,IEA 19667065
GO:0004709 MAP kinase kinase kinase activity IBA,IEA --
GO:0005057 signal transducer activity, downstream of receptor IEA --
genes like me logo Genes that share ontologies with BRAF: view

Phenotypes for BRAF Gene

MGI mutant phenotypes for BRAF:
inferred from 19 alleles
GenomeRNAi human phenotypes for BRAF:
genes like me logo Genes that share phenotypes with BRAF: view

Human Phenotype Ontology for BRAF Gene

HPO Id HPO Name Alternative Ids Definition Synonyms

Animal Models for BRAF Gene

MGI Knock Outs for BRAF:

Clone Products

  • Addgene plasmids for BRAF

Cell Line Products

No data available for Transcription Factor Targets and HOMER Transcription for BRAF Gene

Localization for BRAF Gene

Subcellular locations from UniProtKB/Swiss-Prot for BRAF Gene

Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for BRAF gene
Compartment Confidence
cytosol 5
nucleus 4
plasma membrane 3
mitochondrion 2

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (2)
  • Vesicles (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for BRAF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005622 intracellular IBA --
GO:0005634 nucleus IEA --
GO:0005737 cytoplasm IEA --
GO:0005739 mitochondrion IEA --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with BRAF: view

Pathways & Interactions for BRAF Gene

SuperPathway Contained pathways
1 RET signaling
2 Endometrial cancer
.52
.47
3 Common Cytokine Receptor Gamma-Chain Family Signaling Pathways
4 Negative regulation of FGFR3 signaling
5 IL-2 Pathway
genes like me logo Genes that share pathways with BRAF: view

Pathways by source for BRAF Gene

SIGNOR curated interactions for BRAF Gene

Activates:
Is activated by:
Is inactivated by:

Gene Ontology (GO) - Biological Process for BRAF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000165 MAPK cascade TAS --
GO:0000186 activation of MAPKK activity IEA --
GO:0002318 myeloid progenitor cell differentiation IEA --
GO:0006468 protein phosphorylation IEA,IDA 17563371
GO:0007165 signal transduction IEA --
genes like me logo Genes that share ontologies with BRAF: view

Drugs & Compounds for BRAF Gene

(163) Drugs for BRAF Gene - From: DrugBank, PharmGKB, ClinicalTrials, ApexBio, DGIdb, FDA Approved Drugs, HMDB, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Sorafenib Approved, Investigational Pharma Target, inhibitor, inhibitors Raf kinases and tyrosine kinases inhibitor, RAF, VEGFR2, and PDGFR-beta inhibitors, Kinase Inhibitors, Vascular endothelial growth factor (VEGF) and VEGF receptor (VEGFR) inhibitors 717
Dabrafenib Approved, Investigational Pharma Target, inhibitor, Inhibition BRAF kinase inhibitor, Kinase Inhibitors, Mutant BRAF Kinase 92
Vemurafenib Approved Pharma Target, inhibitor, Biomarker, activator BRAF kinase inhibitor, B-RAF Inhibitors, Kinase Inhibitors, BRAF inhibitors 0
Trametinib Approved Pharma inhibitor, Biomarker MEK 1/2 inhibitor, MEK Inhibitors, Kinase Inhibitors, Mitogen-activated protein/extracellular signal-regulated kinase (MEK) inhibitors 151
regorafenib Approved Pharma Target, inhibitor Inhibitor of VEGFR/PDGFR/FGFR/mutant kit/RET/Raf-1, Kinase Inhibitors, Vascular endothelial growth factor (VEGF) and VEGF receptor (VEGFR) inhibitors 0

(30) Additional Compounds for BRAF Gene - From: Novoseek, Tocris, and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0
Diglycerides Group A
  • 1-Myristoyl-2-pentadecanoyl-sn-glycerol
  • DAG(14:0/15:0)
  • DAG(29:0)
  • DG(14:0/15:0)
  • DG(29:0)
Diglycerides Group B
  • 1-Palmitoleoyl-2-arachidonoyl-sn-glycerol
  • DAG(16:1/20:4)
  • DAG(16:1n7/20:4n6)
  • DAG(16:1w7/20:4w6)
  • DAG(36:5)
Diglycerides Group C
  • 1-Pentadecanoyl-3-behenoyl-sn-glycerol
  • 1-Pentadecanoyl-3-docosanoyl-sn-glycerol
  • DAG(15:0/0:0/22:0)
  • DAG(37:0)
  • DG(37:0)
Diglycerides Group D
  • 1,2-Didocosahexaenoyl-rac-glycerol
  • DAG(22:6/22:6)
  • DAG(22:6n3/22:6n3)
  • DAG(22:6w3/22:6w3)
  • DAG(44:12)

(4) Tocris Compounds for BRAF Gene

Compound Action Cas Number
GW 5074 Potent, selective c-Raf1 kinase inhibitor 220904-83-6
KG 5 PDGFRbeta, B-Raf, c-Raf, FLT3 and KIT inhibitor 877874-85-6
Kobe 0065 H-Ras-cRaf1 interaction inhibitor; inhibits Raf signaling 436133-68-5
ZM 336372 Potent, selective c-Raf inhibitor 208260-29-1

(25) ApexBio Compounds for BRAF Gene

Compound Action Cas Number
AZ 628 Raf kinases,potent and ATP-competitive 878739-06-1
B-Raf IN 1 950736-05-7
B-Raf inhibitor A B-Raf inhibitor 1315330-11-0
BRAF inhibitor Potent B-raf inhibitor 918505-61-0
B-Raf inhibitor 1 1093100-40-3
B-Raf inhibitor 1 dihydrochloride B-Raf inhibitor,potent and selective 1191385-19-9
CEP-32496 BRAF(V600E)inhibitor,highly potent 1188910-76-0
CEP-32496 hydrochloride B-Raf/C-Raf inhibitor,highly potent 1227678-26-3
Dabrafenib (GSK2118436) Inhibitor of BRAF(V600) mutants 1195765-45-7
Dabrafenib Mesylate (GSK-2118436) Inhibitor of BRAF(V600) mutants 1195768-06-9
GDC-0879 B-Raf inhibitor,potent and selective 905281-76-7
HG6-64-1 1315329-43-1
L-779,450 303727-31-3
LGX818 RAF inhibitor,potent and selective 1269440-17-6
LY3009120 pan-RAF and RAF dimer inhibitor 1454682-72-4
ML 786 dihydrochloride Raf kinase inhibitor 1237536-18-3
MLN 2480 Pan-Raf kinase inhibitor,investigational 1096708-71-2
PF-04880594 RAF inhibitor 1111636-35-1
PLX-4720 BRAF kinase inhibitor 918505-84-7
RAF265 Multiple intracellular kinases inhibitor 927880-90-8
SB590885 Potent B-Raf inhibitor 405554-55-4
Sorafenib Raf kinases and tyrosine kinases inhibitor 284461-73-0
Sorafenib Tosylate Raf kinases and tyrosine kinases inhibitor 475207-59-1
TAK-632 Pan-RAF inhibitor,potent and selective 1228591-30-7
Vemurafenib (PLX4032, RG7204) BRAF kinase inhibitor 918504-65-1
genes like me logo Genes that share compounds with BRAF: view

Drug Products