This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been ide... See more...

Aliases for BRAF Gene

Aliases for BRAF Gene

  • B-Raf Proto-Oncogene, Serine/Threonine Kinase 2 3 5
  • V-Raf Murine Sarcoma Viral Oncogene Homolog B1 3 4
  • V-Raf Murine Sarcoma Viral Oncogene Homolog B 2 3
  • Serine/Threonine-Protein Kinase B-Raf 3 4
  • Proto-Oncogene B-Raf 3 4
  • BRAF1 3 4
  • RAFB1 3 4
  • B-Raf Proto-Oncogene Serine/Threonine-Protein Kinase (P94) 3
  • Murine Sarcoma Viral (V-Raf) Oncogene Homolog B1 3
  • B-Raf Serine/Threonine-Protein 3
  • 94 KDa B-Raf Protein 3
  • EC 2.7.11.1 4
  • B-RAF1 3
  • B-Raf 3
  • NS7 3
  • P94 4

External Ids for BRAF Gene

Previous GeneCards Identifiers for BRAF Gene

  • GC07M138705
  • GC07M139764
  • GC07M139841
  • GC07M139754
  • GC07M139887
  • GC07M140080
  • GC07M140424
  • GC07M134729

Summaries for BRAF Gene

Entrez Gene Summary for BRAF Gene

  • This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]

CIViC Summary for BRAF Gene

  • BRAF mutations are found to be recurrent in many cancer types. Of these, the mutation of valine 600 to glutamic acid (V600E) is the most prevalent. V600E has been determined to be an activating mutation, and cells that harbor it, along with other V600 mutations are sensitive to the BRAF inhibitor dabrafenib. It is also common to use MEK inhibition as a substitute for BRAF inhibitors, and the MEK inhibitor trametinib has seen some success in BRAF mutant melanomas. BRAF mutations have also been correlated with poor prognosis in many cancer types, although there is at least one study that questions this conclusion in papillary thyroid cancer.

GeneCards Summary for BRAF Gene

BRAF (B-Raf Proto-Oncogene, Serine/Threonine Kinase) is a Protein Coding gene. Diseases associated with BRAF include Cardiofaciocutaneous Syndrome 1 and Lung Cancer. Among its related pathways are MAPK-Erk Pathway and RANK Signaling in Osteoclasts. Gene Ontology (GO) annotations related to this gene include calcium ion binding and transferase activity, transferring phosphorus-containing groups. An important paralog of this gene is RAF1.

UniProtKB/Swiss-Prot Summary for BRAF Gene

  • Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Tocris Summary for BRAF Gene

  • Raf kinases, a family of three serine/threonine kinases, are part of the Ras-MAPK signaling cascade and phosphorylate MEK. Upon growth factor stimulation, Raf-1 (c-Raf) is activated by GTP-bound Ras and recruited to the cell membrane. This activation process is tightly regulated.

Gene Wiki entry for BRAF Gene

PharmGKB "VIP" Summary for BRAF Gene

Additional gene information for BRAF Gene

No data available for Rfam classification and piRNA Summary for BRAF Gene

Genomics for BRAF Gene

GeneHancer (GH) Regulatory Elements for BRAF Gene

Promoters and enhancers for BRAF Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around BRAF on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the BRAF gene promoter:
  • AP-1
  • ATF-2
  • c-Jun
  • C/EBPalpha
  • E2F
  • E2F-1
  • E2F-2
  • p53
  • STAT3

Genomic Locations for BRAF Gene

Genomic Locations for BRAF Gene
chr7:140,715,951-140,924,928
(GRCh38/hg38)
Size:
208,978 bases
Orientation:
Minus strand
chr7:140,419,127-140,624,564
(GRCh37/hg19)
Size:
205,438 bases
Orientation:
Minus strand

Genomic View for BRAF Gene

Genes around BRAF on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
BRAF Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for BRAF Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for BRAF Gene

Proteins for BRAF Gene

  • Protein details for BRAF Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P15056-BRAF_HUMAN
    Recommended name:
    Serine/threonine-protein kinase B-raf
    Protein Accession:
    P15056
    Secondary Accessions:
    • A4D1T4
    • B6HY61
    • B6HY62
    • B6HY63
    • B6HY64
    • B6HY65
    • B6HY66
    • Q13878
    • Q3MIN6
    • Q9UDP8
    • Q9Y6T3

    Protein attributes for BRAF Gene

    Size:
    766 amino acids
    Molecular mass:
    84437 Da
    Cofactor:
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
    Quaternary structure:
    • Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-753. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1 (By similarity). Interacts with DGKH (PubMed:19710016). Interacts with PRMT5 (PubMed:21917714). Interacts with KSR2 (PubMed:21441910). Interacts with AKAP13, MAP2K1 and KSR1. Identified in a complex with AKAP13, MAP2K1 and KSR1 (PubMed:21102438). Interacts with FNIP1 and FNIP2 (PubMed:27353360).
    SequenceCaution:
    • Sequence=AAD43193.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=CAQ43111.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAQ43112.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAQ43113.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAQ43114.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAQ43115.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAQ43116.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for BRAF Gene

neXtProt entry for BRAF Gene

Post-translational modifications for BRAF Gene

  • Phosphorylation at Ser-365 by SGK1 inhibits its activity.
  • Methylation at Arg-671 decreases stability and kinase activity.
  • Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.
  • Ubiquitination at Lys578
  • Modification sites at PhosphoSitePlus

Antibody Products

  • Abcam antibodies for BRAF

Protein Products

  • Abcam proteins for BRAF

No data available for DME Specific Peptides for BRAF Gene

Domains & Families for BRAF Gene

Gene Families for BRAF Gene

HGNC:
IUPHAR :
Human Protein Atlas (HPA):
  • Cancer-related genes
  • Disease related genes
  • Enzymes
  • FDA approved drug targets
  • Predicted intracellular proteins

Suggested Antigen Peptide Sequences for BRAF Gene

GenScript: Design optimal peptide antigens:
  • v-Raf murine sarcoma viral oncogene homolog B1 (BRAF_HUMAN)
  • Serine/threonine-protein kinase B-raf variant (E2I6F4_HUMAN)
  • BRAF protein (E5FF37_HUMAN)
  • V-raf murine sarcoma viral oncogene-like protein B1 (F1CC75_HUMAN)
  • B-Raf protein (Q9NY11_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P15056

UniProtKB/Swiss-Prot:

BRAF_HUMAN :
  • Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Family:
  • Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
genes like me logo Genes that share domains with BRAF: view

Function for BRAF Gene

Molecular function for BRAF Gene

UniProtKB/Swiss-Prot Function:
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1; Evidence=. ;.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1; Evidence=. ;.
UniProtKB/Swiss-Prot EnzymeRegulation:
Activity is increased by EGF and HGF.
GENATLAS Biochemistry:
murine sarcoma 3611 viral (v-raf) oncogene homolog B1 (Rmil),expressed in cerebrum and testis,fused with TIF1A to form the oncoprotein T18

Enzyme Numbers (IUBMB) for BRAF Gene

Phenotypes From GWAS Catalog for BRAF Gene

Gene Ontology (GO) - Molecular Function for BRAF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0004672 protein kinase activity IDA,IEA 17563371
GO:0004674 protein serine/threonine kinase activity IDA,IEA 19667065
GO:0004709 MAP kinase kinase kinase activity IEA --
GO:0004713 protein tyrosine kinase activity IEA --
genes like me logo Genes that share ontologies with BRAF: view

Phenotypes for BRAF Gene

MGI mutant phenotypes for BRAF:
inferred from 19 alleles
GenomeRNAi human phenotypes for BRAF:
genes like me logo Genes that share phenotypes with BRAF: view

Human Phenotype Ontology for BRAF Gene

HPO Id HPO Name Alternative Ids Definition Synonyms

Animal Models for BRAF Gene

MGI Knock Outs for BRAF:

Animal Model Products

CRISPR Products

Clone Products

  • Addgene plasmids for BRAF

No data available for Transcription Factor Targets and HOMER Transcription for BRAF Gene

Localization for BRAF Gene

Subcellular locations from UniProtKB/Swiss-Prot for BRAF Gene

Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for BRAF gene
Compartment Confidence
cytosol 5
nucleus 4
plasma membrane 3
mitochondrion 3

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (2)
  • Vesicles (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for BRAF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005737 cytoplasm IEA --
GO:0005739 mitochondrion IBA 21873635
GO:0005829 cytosol TAS --
GO:0005886 plasma membrane IBA,TAS --
genes like me logo Genes that share ontologies with BRAF: view

Pathways & Interactions for BRAF Gene

PathCards logo

SuperPathways for BRAF Gene

SuperPathway Contained pathways
1 RET signaling
2 Common Cytokine Receptor Gamma-Chain Family Signaling Pathways
3 Negative regulation of FGFR1 signaling
4 Endometrial cancer
5 IL-2 Pathway
.59
.59
.54
.49
.44
.43
.34
genes like me logo Genes that share pathways with BRAF: view

Pathways by source for BRAF Gene

3 Tocris pathways for BRAF Gene
59 Qiagen pathways for BRAF Gene
  • Activation of cAMP-Dependent PKA
  • Activation of PKA through GPCR
  • Aldosterone Signaling in Epithelial Cells
  • Alpha-Adrenergic Signaling
  • Beta-Adrenergic Signaling
1 Cell Signaling Technology pathway for BRAF Gene
2 GeneTex pathways for BRAF Gene

SIGNOR curated interactions for BRAF Gene

Activates:
Inactivates:
Is activated by:
Is inactivated by:

Gene Ontology (GO) - Biological Process for BRAF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000165 MAPK cascade TAS --
GO:0000186 activation of MAPKK activity IEA --
GO:0002318 myeloid progenitor cell differentiation IEA --
GO:0006468 protein phosphorylation IDA,IEA 17563371
GO:0007165 signal transduction IEA --
genes like me logo Genes that share ontologies with BRAF: view

Drugs & Compounds for BRAF Gene

(176) Drugs for BRAF Gene - From: DrugBank, PharmGKB, ClinicalTrials, ApexBio, DGIdb, HMDB, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Sorafenib Approved, Investigational Pharma Target, inhibitor, inhibitors Raf kinases and tyrosine kinases inhibitor, RAF, VEGFR2, and PDGFR-beta inhibitors, Kinase Inhibitors, Vascular endothelial growth factor (VEGF) and VEGF receptor (VEGFR) inhibitors 762
Dabrafenib Approved, Investigational Pharma Target, antagonist, inhibitor, Inhibition BRAF kinase inhibitor, Kinase Inhibitors, Mutant BRAF Kinase 108
Vemurafenib Approved Pharma Target, inhibitor, Biomarker, activator BRAF kinase inhibitor, B-RAF Inhibitors, Kinase Inhibitors, BRAF inhibitors 130
Trametinib Approved Pharma inhibitor, Biomarker MEK 1/2 inhibitor, MEK Inhibitors, Kinase Inhibitors, Mitogen-activated protein/extracellular signal-regulated kinase (MEK) inhibitors 179
encorafenib Approved, Investigational Pharma Target, inhibitor 0

(31) Additional Compounds for BRAF Gene - From: HMDB, Novoseek, and Tocris