AZI2, or NAP1, contributes to the activation of NFKB (see MIM 164011)-dependent gene expression by activating IKK-related kinases, such as NAK (TBK1; MIM 604834) (Fujita et al., 2003 [PubMed 14560022]).[supplied by OMIM, Mar 2008] See more...

Aliases for AZI2 Gene

Aliases for AZI2 Gene

  • 5-Azacytidine Induced 2 2 3 5
  • NAP1 2 3 4
  • NF-Kappa-B-Activating Kinase-Associated Protein 1 3 4
  • 5-Azacytidine-Induced Protein 2 3 4
  • TILP 3 4
  • AZ2 2 3
  • 5-Azacytidine Induced Gene 2 3
  • NAK-Associated Protein 1 3
  • Nak-Associated Protein 1 4
  • FLJ21939 2
  • TBKBP2 4
  • AZI2 5

External Ids for AZI2 Gene

Previous GeneCards Identifiers for AZI2 Gene

  • GC03M028340

Summaries for AZI2 Gene

Entrez Gene Summary for AZI2 Gene

  • AZI2, or NAP1, contributes to the activation of NFKB (see MIM 164011)-dependent gene expression by activating IKK-related kinases, such as NAK (TBK1; MIM 604834) (Fujita et al., 2003 [PubMed 14560022]).[supplied by OMIM, Mar 2008]

GeneCards Summary for AZI2 Gene

AZI2 (5-Azacytidine Induced 2) is a Protein Coding gene. Diseases associated with AZI2 include Vaccinia and Urethral Diverticulum. Among its related pathways are RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways. An important paralog of this gene is TBKBP1.

UniProtKB/Swiss-Prot Summary for AZI2 Gene

  • Adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFNB promoter activation via TICAM1.

Gene Wiki entry for AZI2 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for AZI2 Gene

Genomics for AZI2 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for AZI2 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH03J028346 Promoter/Enhancer 2.2 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 255 +1.0 977 4.6 BCLAF1 SP1 ZNF207 IKZF1 ZNF600 SIX5 ATF2 POLR2A CEBPA NFIC AZI2 ENSG00000283563 HSALNG0024848 ZCWPW2 HSALNG0024847 CMC1
GH03J028326 Enhancer 0.9 Ensembl ENCODE 11.6 +22.7 22677 2.4 SIN3A TRIM22 NR2C1 FOXA2 RBM22 SMARCA5 NFE2 BHLHE40 ELF1 ZNF24 HSALNG0024845 lnc-EOMES-5 AZI2 ZCWPW2 CMC1 piR-38351-297
GH03J028308 Enhancer 0.8 Ensembl ENCODE 12.7 +41.1 41077 1.2 CEBPA ATF3 CEBPB EP300 IRF9 ATF4 ATF2 TRIM24 ATF7 PKNOX1 AZI2 ZCWPW2 CMC1 HSALNG0024845 piR-38351-297 NONHSAG034650.2
GH03J027665 Enhancer 0.6 ENCODE 10.4 +682.5 682548 1.5 POLR2A ETS1 SIN3A RAD21 SPI1 MYC MAX IKZF1 BMI1 RFX1 RNU1-96P ZCWPW2 AZI2 ENSG00000271943 LINC02084 piR-61945-343 EOMES SLC4A7
GH03J028292 Enhancer 0.4 ENCODE 11.3 +54.5 54538 3.6 ZNF133 CEBPB CEBPG AZI2 ZCWPW2 CMC1 piR-38351-297 HSALNG0024845 NONHSAG034650.2
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around AZI2 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for AZI2

Top Transcription factor binding sites by QIAGEN in the AZI2 gene promoter:
  • AREB6
  • IRF-1
  • NCX
  • Pax-5
  • POU2F1
  • POU2F1a
  • SEF-1 (1)
  • SRY
  • STAT5A

Genomic Locations for AZI2 Gene

Latest Assembly
chr3:28,315,003-28,349,278
(GRCh38/hg38)
Size:
34,276 bases
Orientation:
Minus strand

Previous Assembly
chr3:28,362,538-28,390,315
(GRCh37/hg19 by Entrez Gene)
Size:
27,778 bases
Orientation:
Minus strand

chr3:28,356,494-28,390,618
(GRCh37/hg19 by Ensembl)
Size:
34,125 bases
Orientation:
Minus strand

Genomic View for AZI2 Gene

Genes around AZI2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
AZI2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for AZI2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for AZI2 Gene

Proteins for AZI2 Gene

  • Protein details for AZI2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9H6S1-AZI2_HUMAN
    Recommended name:
    5-azacytidine-induced protein 2
    Protein Accession:
    Q9H6S1
    Secondary Accessions:
    • A8K3M2
    • C9JB40
    • H7BXU6
    • Q86W99
    • Q9BQF1

    Protein attributes for AZI2 Gene

    Size:
    392 amino acids
    Molecular mass:
    44935 Da
    Quaternary structure:
    • Homodimer (By similarity). Interacts with IKBKE (PubMed:14560022, PubMed:17568778). Interacts with TBK1 (PubMed:14560022, PubMed:21931631, PubMed:29251827). Interacts with TICAM1 (PubMed:15611223).
    • (Microbial infection) Interacts with vaccinia virus protein C6 (PubMed:21931555).

    Three dimensional structures from OCA and Proteopedia for AZI2 Gene

    Alternative splice isoforms for AZI2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for AZI2 Gene

Post-translational modifications for AZI2 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for AZI2 Gene

Domains & Families for AZI2 Gene

Gene Families for AZI2 Gene

Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for AZI2 Gene

InterPro:

Suggested Antigen Peptide Sequences for AZI2 Gene

GenScript: Design optimal peptide antigens:
  • TILP (AZI2_HUMAN)
genes like me logo Genes that share domains with AZI2: view

No data available for Graphical View of Domain Structure and UniProtKB/Swiss-Prot for AZI2 Gene

Function for AZI2 Gene

Molecular function for AZI2 Gene

UniProtKB/Swiss-Prot Function:
Adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFNB promoter activation via TICAM1.

Gene Ontology (GO) - Molecular Function for AZI2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 14743216
genes like me logo Genes that share ontologies with AZI2: view
genes like me logo Genes that share phenotypes with AZI2: view

Animal Models for AZI2 Gene

MGI Knock Outs for AZI2:

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for AZI2

Clone products for research

No data available for Enzyme Numbers (IUBMB) , Phenotypes From GWAS Catalog , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for AZI2 Gene

Localization for AZI2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for AZI2 Gene

Cytoplasm.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for AZI2 gene
Compartment Confidence
nucleus 3
cytosol 2
plasma membrane 1
extracellular 1
cytoskeleton 1
mitochondrion 1
peroxisome 1
endoplasmic reticulum 1
endosome 1
lysosome 1

Gene Ontology (GO) - Cellular Components for AZI2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IBA 21873635
genes like me logo Genes that share ontologies with AZI2: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for AZI2 Gene

Pathways & Interactions for AZI2 Gene

genes like me logo Genes that share pathways with AZI2: view

Pathways by source for AZI2 Gene

1 BioSystems pathway for AZI2 Gene
1 KEGG pathway for AZI2 Gene

Gene Ontology (GO) - Biological Process for AZI2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000278 mitotic cell cycle IEA --
GO:0007249 I-kappaB kinase/NF-kappaB signaling IBA 21873635
GO:0016032 viral process IEA --
GO:0042110 T cell activation IEA --
GO:0044565 dendritic cell proliferation IEA --
genes like me logo Genes that share ontologies with AZI2: view

No data available for SIGNOR curated interactions for AZI2 Gene

Drugs & Compounds for AZI2 Gene

(1) Additional Compounds for AZI2 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with AZI2: view

Transcripts for AZI2 Gene

mRNA/cDNA for AZI2 Gene

4 REFSEQ mRNAs :
14 NCBI additional mRNA sequence :
14 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for AZI2

Clone products for research

Alternative Splicing Database (ASD) splice patterns (SP) for AZI2 Gene

ExUns: 1a · 1b · 1c · 1d · 1e ^ 2 ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8a · 8b ^ 9 ^ 10 ^ 11a · 11b · 11c ^ 12a · 12b ^ 13
SP1: - - - - - - -
SP2: - - -
SP3: -
SP4: - - - - - -
SP5: -
SP6: - - - - - - -
SP7: -
SP8: - - - -
SP9:
SP10: - -

Relevant External Links for AZI2 Gene

GeneLoc Exon Structure for
AZI2

Expression for AZI2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for AZI2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for AZI2 Gene

This gene is overexpressed in Lung (38.8) and NK cells (24.8).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for AZI2 Gene



Transcriptomic regulation report from SPP (The Signaling Pathways Project) for AZI2

SOURCE GeneReport for Unigene cluster for AZI2 Gene:

Hs.706676

mRNA Expression by UniProt/SwissProt for AZI2 Gene:

Q9H6S1-AZI2_HUMAN
Tissue specificity: Widely expressed. Abundant expression seen in the pancreas and testis.

Evidence on tissue expression from TISSUES for AZI2 Gene

  • Liver(4.3)
  • Nervous system(4)
genes like me logo Genes that share expression patterns with AZI2: view

No data available for mRNA differential expression in normal tissues , Protein tissue co-expression partners and Phenotype-based relationships between genes and organs from Gene ORGANizer for AZI2 Gene

Orthologs for AZI2 Gene

This gene was present in the common ancestor of chordates.

Orthologs for AZI2 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia AZI2 29 30
  • 99.83 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia AZI2 29 30
  • 92.52 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia AZI2 29 30
  • 88.95 (n)
OneToOne
Mouse
(Mus musculus)
Mammalia Azi2 29 16 30
  • 84.86 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Azi2 29
  • 81.67 (n)
Oppossum
(Monodelphis domestica)
Mammalia AZI2 30
  • 79 (a)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia AZI2 30
  • 74 (a)
OneToOne
Chicken
(Gallus gallus)
Aves AZI2 29 30
  • 69.04 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia AZI2 30
  • 57 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia azi2 29
  • 60.3 (n)
Zebrafish
(Danio rerio)
Actinopterygii azi2 29 30
  • 52.33 (n)
OneToOne
Species where no ortholog for AZI2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for AZI2 Gene

ENSEMBL:
Gene Tree for AZI2 (if available)
TreeFam:
Gene Tree for AZI2 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for AZI2: view image
Alliance of Genome Resources:
Additional Orthologs for AZI2

Paralogs for AZI2 Gene

Paralogs for AZI2 Gene

(1) SIMAP similar genes for AZI2 Gene using alignment to 4 proteins:

  • AZI2_HUMAN
  • C9JGA2_HUMAN
  • C9JVK8_HUMAN
  • H7C1Z1_HUMAN

Pseudogenes.org Pseudogenes for AZI2 Gene

genes like me logo Genes that share paralogs with AZI2: view

Variants for AZI2 Gene

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for AZI2 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for AZI2 Gene

Variant ID Type Subtype PubMed ID
dgv1365e212 CNV loss 25503493
esv3568668 CNV loss 25503493
esv996568 OTHER inversion 20482838

Variation tolerance for AZI2 Gene

Residual Variation Intolerance Score: 51.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.52; 11.30% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for AZI2 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
AZI2
Leiden Open Variation Database (LOVD)
AZI2

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for AZI2 Gene

Disorders for AZI2 Gene

MalaCards: The human disease database

(2) MalaCards diseases for AZI2 Gene - From: COP and GCD

Disorder Aliases PubMed IDs
vaccinia
urethral diverticulum
- elite association - COSMIC cancer census association via MalaCards
Search AZI2 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for AZI2

genes like me logo Genes that share disorders with AZI2: view

No data available for UniProtKB/Swiss-Prot and Genatlas for AZI2 Gene

Publications for AZI2 Gene

  1. Cutting Edge: NF-kappaB-activating kinase-associated protein 1 participates in TLR3/Toll-IL-1 homology domain-containing adapter molecule-1-mediated IFN regulatory factor 3 activation. (PMID: 15611223) Sasai M … Seya T (Journal of immunology (Baltimore, Md. : 1950) 2005) 3 4 22
  2. Identification of NAP1, a regulatory subunit of IkappaB kinase-related kinases that potentiates NF-kappaB signaling. (PMID: 14560022) Fujita F … Nakanishi M (Molecular and cellular biology 2003) 3 4 22
  3. Quantitative Proteomics Identified TTC4 as a TBK1 Interactor and a Positive Regulator of SeV-Induced Innate Immunity. (PMID: 29251827) Shang J … Guo L (Proteomics 2018) 3 4
  4. Vaccinia virus protein C6 is a virulence factor that binds TBK-1 adaptor proteins and inhibits activation of IRF3 and IRF7. (PMID: 21931555) Unterholzner L … Bowie AG (PLoS pathogens 2011) 3 4
  5. SINTBAD, a novel component of innate antiviral immunity, shares a TBK1-binding domain with NAP1 and TANK. (PMID: 17568778) Ryzhakov G … Randow F (The EMBO journal 2007) 3 4

Products for AZI2 Gene

Sources for AZI2 Gene