This gene encodes one of seven subunits of the human Arp2/3 protein complex. This complex controls actin polymerization in cells and has been conserved throughout eukaryotic evolution. This gene encodes the p20 subunit, which is necessary for actin nucleation and high-affinity binding to F-actin. Alternative splicing results in multiple transcript variants. Naturally occurring ... See more...

Aliases for ARPC4 Gene

Aliases for ARPC4 Gene

  • Actin Related Protein 2/3 Complex Subunit 4 2 3 5
  • ARC20 2 3 4
  • Actin-Related Protein 2/3 Complex Subunit 4 3 4
  • Arp2/3 Protein Complex Subunit P20 2 3
  • Arp2/3 Complex 20 KDa Subunit 3 4
  • P20-ARC 3 4
  • Actin Related Protein 2/3 Complex, Subunit 4 (20 KD) 2
  • Actin Related Protein 2/3 Complex, Subunit 4, 20kDa 3
  • Actin Related Protein 2/3 Complex Subunit 4, 20kDa 2
  • P20-Arc 2
  • ARPC4 5

External Ids for ARPC4 Gene

Previous GeneCards Identifiers for ARPC4 Gene

  • GC03P009763
  • GC03P009826
  • GC03P009809
  • GC03P009750
  • GC03P009834
  • GC03P009775

Summaries for ARPC4 Gene

Entrez Gene Summary for ARPC4 Gene

  • This gene encodes one of seven subunits of the human Arp2/3 protein complex. This complex controls actin polymerization in cells and has been conserved throughout eukaryotic evolution. This gene encodes the p20 subunit, which is necessary for actin nucleation and high-affinity binding to F-actin. Alternative splicing results in multiple transcript variants. Naturally occurring read-through transcription exists between this gene and the downstream tubulin tyrosine ligase-like family, member 3 (TTLL3), which results in the production of a fusion protein. [provided by RefSeq, Nov 2010]

GeneCards Summary for ARPC4 Gene

ARPC4 (Actin Related Protein 2/3 Complex Subunit 4) is a Protein Coding gene. Diseases associated with ARPC4 include Patellar Tendinitis. Among its related pathways are Signaling by GPCR and Immune response CCR3 signaling in eosinophils. Gene Ontology (GO) annotations related to this gene include enzyme binding and structural constituent of cytoskeleton.

UniProtKB/Swiss-Prot Summary for ARPC4 Gene

  • Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).

Gene Wiki entry for ARPC4 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for ARPC4 Gene

Genomics for ARPC4 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for ARPC4 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH03J009791 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE CraniofacialAtlas 250.7 +1.1 1108 4.8 SP1 NCOR1 ZNF207 MYC IKZF1 SIX5 JUND ZNF580 POLR2A ATF3 TADA3 ARPC4 ARPC4-TTLL3 HSALNG0024053 lnc-CAMK1-1 BRPF1 SETD5 RAD18 BRK1 TTLL3
GH03J009803 Promoter/Enhancer 0.6 EPDnew Ensembl 250.4 +11.4 11406 0.6 POLR2A ARPC4 TTLL3 TADA3 HSALNG0024053 ARPC4-TTLL3 MG828730-038
GH03J009808 Promoter 0.5 EPDnew 250.4 +14.5 14536 0.1 RBM22 POLR2A TTLL3 ARPC4 TADA3 HSALNG0024053 ARPC4-TTLL3 MG828730-038
GH03J009806 Promoter 0.5 EPDnew 250.4 +14.3 14293 0.1 RBM22 POLR2A TTLL3 ARPC4 TADA3 HSALNG0024053 ARPC4-TTLL3 MG828730-038
GH03J009797 Promoter 0.4 EPDnew 250.4 +5.1 5130 0.1 POLR2A ARPC4 HSALNG0024053 TADA3 ARPC4-TTLL3 TTLL3 MG828730-038
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around ARPC4 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for ARPC4

Top Transcription factor binding sites by QIAGEN in the ARPC4 gene promoter:
  • CREB
  • deltaCREB
  • E2F
  • E2F-1
  • HEN1
  • NF-1
  • POU2F1
  • POU2F1a

Genomic Locations for ARPC4 Gene

Latest Assembly
chr3:9,792,495-9,807,101
(GRCh38/hg38)
Size:
14,607 bases
Orientation:
Plus strand

Previous Assembly
chr3:9,834,202-9,848,785
(GRCh37/hg19 by Entrez Gene)
Size:
14,584 bases
Orientation:
Plus strand

chr3:9,834,179-9,849,410
(GRCh37/hg19 by Ensembl)
Size:
15,232 bases
Orientation:
Plus strand

Genomic View for ARPC4 Gene

Genes around ARPC4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ARPC4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ARPC4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ARPC4 Gene

Proteins for ARPC4 Gene

  • Protein details for ARPC4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P59998-ARPC4_HUMAN
    Recommended name:
    Actin-related protein 2/3 complex subunit 4
    Protein Accession:
    P59998
    Secondary Accessions:
    • C9JWM7
    • E7ETI0
    • F6TTL5
    • O15509
    • Q6P0W5
    • Q96QJ3

    Protein attributes for ARPC4 Gene

    Size:
    168 amino acids
    Molecular mass:
    19667 Da
    Quaternary structure:
    • Component of the Arp2/3 complex composed of ACTR2/ARP2, ACTR3/ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.
    SequenceCaution:
    • Sequence=AAH12596.3; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for ARPC4 Gene

    Alternative splice isoforms for ARPC4 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for ARPC4 Gene

Post-translational modifications for ARPC4 Gene

  • Ubiquitination at Lys44
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for ARPC4 Gene

Domains & Families for ARPC4 Gene

Gene Families for ARPC4 Gene

HGNC:
Human Protein Atlas (HPA):
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for ARPC4 Gene

InterPro:
Blocks:
  • ARP23 complex 20 kDa subunit

Suggested Antigen Peptide Sequences for ARPC4 Gene

GenScript: Design optimal peptide antigens:
  • Arp2/3 complex 20 kDa subunit (ARPC4_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P59998

UniProtKB/Swiss-Prot:

ARPC4_HUMAN :
  • Belongs to the ARPC4 family.
Family:
  • Belongs to the ARPC4 family.
genes like me logo Genes that share domains with ARPC4: view

Function for ARPC4 Gene

Molecular function for ARPC4 Gene

UniProtKB/Swiss-Prot Function:
Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).

Phenotypes From GWAS Catalog for ARPC4 Gene

Gene Ontology (GO) - Molecular Function for ARPC4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003779 actin binding IEA --
GO:0005200 structural constituent of cytoskeleton IDA 11741539
GO:0005515 protein binding IPI 11162547
GO:0019899 enzyme binding IPI 23921388
GO:0030674 contributes_to protein binding, bridging IPI 11162547
genes like me logo Genes that share ontologies with ARPC4: view
genes like me logo Genes that share phenotypes with ARPC4: view

Animal Models for ARPC4 Gene

MGI Knock Outs for ARPC4:
  • Arpc4 Arpc4<tm1a(EUCOMM)Wtsi>

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ARPC4

Clone products for research

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for ARPC4 Gene

Localization for ARPC4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ARPC4 Gene

Cytoplasm, cytoskeleton. Cell projection. Nucleus.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ARPC4 gene
Compartment Confidence
cytoskeleton 5
extracellular 4
nucleus 4
cytosol 4
plasma membrane 1
mitochondrion 1
endoplasmic reticulum 1
endosome 1
lysosome 1
golgi apparatus 1

Gene Ontology (GO) - Cellular Components for ARPC4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus ISS --
GO:0005737 cytoplasm IEA --
GO:0005829 cytosol TAS --
GO:0005856 cytoskeleton IEA --
GO:0005885 Arp2/3 protein complex TAS 9230079
genes like me logo Genes that share ontologies with ARPC4: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for ARPC4 Gene

Pathways & Interactions for ARPC4 Gene

PathCards logo

SuperPathways for ARPC4 Gene

SuperPathway Contained pathways
1 G-protein signaling RAC1 in cellular process
.37
.37
2 EPH-Ephrin signaling
.35
3 Regulation of actin dynamics for phagocytic cup formation
4 Cytoskeleton remodeling Regulation of actin cytoskeleton by Rho GTPases
.49
.49
.37
5 Development Slit-Robo signaling
.44
.44
genes like me logo Genes that share pathways with ARPC4: view

Pathways by source for ARPC4 Gene

14 GeneGo (Thomson Reuters) pathways for ARPC4 Gene
  • Cell adhesion Integrin-mediated cell adhesion and migration
  • Cell adhesion_Cadherin-mediated cell adhesion
  • Cytoskeleton remodeling CDC42 in cellular processes
  • Cytoskeleton remodeling Fibronectin-binding integrins in cell motility
  • Cytoskeleton remodeling Integrin outside-in signaling
1 Qiagen pathway for ARPC4 Gene
  • Salmonella Infection

Gene Ontology (GO) - Biological Process for ARPC4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0030041 actin filament polymerization IEA --
GO:0030833 regulation of actin filament polymerization IEA --
GO:0034314 Arp2/3 complex-mediated actin nucleation IDA 11741539
GO:0038096 Fc-gamma receptor signaling pathway involved in phagocytosis TAS --
GO:0045010 actin nucleation NAS 11162547
genes like me logo Genes that share ontologies with ARPC4: view

No data available for SIGNOR curated interactions for ARPC4 Gene

Drugs & Compounds for ARPC4 Gene

(2) Drugs for ARPC4 Gene - From: DrugBank

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
(2S)-2-(3-bromophenyl)-3-(5-chloro-2-hydroxyphenyl)-1,3-thiazolidin-4-one Experimental Pharma Target 0
N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide Experimental Pharma Target 0
genes like me logo Genes that share compounds with ARPC4: view

Transcripts for ARPC4 Gene

mRNA/cDNA for ARPC4 Gene

4 REFSEQ mRNAs :
16 NCBI additional mRNA sequence :
8 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ARPC4

Clone products for research

Alternative Splicing Database (ASD) splice patterns (SP) for ARPC4 Gene

No ASD Table

Relevant External Links for ARPC4 Gene

GeneLoc Exon Structure for
ARPC4

Expression for ARPC4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for ARPC4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for ARPC4 Gene

This gene is overexpressed in Whole Blood (x7.3).

Protein differential expression in normal tissues from HIPED for ARPC4 Gene

This gene is overexpressed in NK cells (10.1) and Tlymphocyte (7.5).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for ARPC4 Gene



Protein tissue co-expression partners for ARPC4 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for ARPC4

SOURCE GeneReport for Unigene cluster for ARPC4 Gene:

Hs.323342

Evidence on tissue expression from TISSUES for ARPC4 Gene

  • Nervous system(4.9)
  • Blood(4.5)
  • Intestine(3)
  • Skin(2.6)
  • Lymph node(2.6)
  • Spleen(2.4)
  • Kidney(2.3)
  • Bone marrow(2.3)
  • Muscle(2.3)
  • Heart(2)
genes like me logo Genes that share expression patterns with ARPC4: view

No data available for mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for ARPC4 Gene

Orthologs for ARPC4 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for ARPC4 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia ARPC4 29 30
  • 99.82 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia ARPC4 30
  • 99 (a)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia ARPC4 30
  • 99 (a)
OneToOne
Dog
(Canis familiaris)
Mammalia ARPC4 30
  • 99 (a)
OneToOne
Cow
(Bos Taurus)
Mammalia ARPC4 29 30
  • 94.25 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Arpc4 29
  • 92.46 (n)
Mouse
(Mus musculus)
Mammalia Arpc4 29 16 30
  • 92.06 (n)
OneToOne
Chicken
(Gallus gallus)
Aves ARPC4 29 30
  • 85.12 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia ARPC4 30
  • 97 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia arpc4 29
  • 82.54 (n)
African clawed frog
(Xenopus laevis)
Amphibia Xl.24361 29
Zebrafish
(Danio rerio)
Actinopterygii arpc4l 30
  • 98 (a)
OneToMany
arpc4 29 30
  • 83.53 (n)
OneToMany
Fruit Fly
(Drosophila melanogaster)
Insecta Arc-p20 30
  • 82 (a)
OneToOne
CG5972 31
  • 82 (a)
Arpc4 29
  • 71.83 (n)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP001306 29
  • 75 (n)
Worm
(Caenorhabditis elegans)
Secernentea C35D10.16 31
  • 77 (a)
arx-6 29 30
  • 66.27 (n)
OneToOne
A. gosspyii yeast
(Eremothecium gossypii)
Saccharomycetes AGOS_ACL092C 29
  • 63.07 (n)
K. Lactis Yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0E18217g 29
  • 62.67 (n)
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes ARC19 29 30
  • 60.08 (n)
OneToOne
Thale Cress
(Arabidopsis thaliana)
eudicotyledons ARPC4 29
  • 60.81 (n)
Rice
(Oryza sativa)
Liliopsida Os03g0848300 29
  • 66.06 (n)
Os.16908 29
Barley
(Hordeum vulgare)
Liliopsida Hv.3712 29
Corn
(Zea mays)
Liliopsida Zm.12074 29
Sea Squirt
(Ciona savignyi)
Ascidiacea CSA.6209 30
  • 85 (a)
OneToOne
Bread mold
(Neurospora crassa)
Ascomycetes NCU01918 29
  • 65.08 (n)
Fission Yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes arc4 29
  • 60.52 (n)
Sea Vase
(Ciona intestinalis)
Ascidiacea Cin.6747 29
Species where no ortholog for ARPC4 was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for ARPC4 Gene

ENSEMBL:
Gene Tree for ARPC4 (if available)
TreeFam:
Gene Tree for ARPC4 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for ARPC4: view image
Alliance of Genome Resources:
Additional Orthologs for ARPC4

Paralogs for ARPC4 Gene

(1) SIMAP similar genes for ARPC4 Gene using alignment to 4 proteins:

  • ARPC4_HUMAN
  • F8WDD7_HUMAN
  • F8WDW3_HUMAN
  • R4GN08_HUMAN
genes like me logo Genes that share paralogs with ARPC4: view

No data available for Paralogs for ARPC4 Gene

Variants for ARPC4 Gene

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for ARPC4 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for ARPC4 Gene

Variant ID Type Subtype PubMed ID
nsv1010768 CNV gain 25217958
nsv834615 CNV loss 17160897

Variation tolerance for ARPC4 Gene

Residual Variation Intolerance Score: 42.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.14; 3.14% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ARPC4 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
ARPC4
Leiden Open Variation Database (LOVD)
ARPC4

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for ARPC4 Gene

Disorders for ARPC4 Gene

MalaCards: The human disease database

(1) MalaCards diseases for ARPC4 Gene - From: COP

Disorder Aliases PubMed IDs
patellar tendinitis
  • patellar tendonitis
- elite association - COSMIC cancer census association via MalaCards
Search ARPC4 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for ARPC4

genes like me logo Genes that share disorders with ARPC4: view

No data available for UniProtKB/Swiss-Prot and Genatlas for ARPC4 Gene

Publications for ARPC4 Gene

  1. Mammalian actin-related protein 2/3 complex localizes to regions of lamellipodial protrusion and is composed of evolutionarily conserved proteins. (PMID: 9359840) Machesky LM … Segal AW (The Biochemical journal 1997) 2 3 4
  2. The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. (PMID: 9230079) Welch MD … Mitchison TJ (The Journal of cell biology 1997) 2 3 4
  3. An actin-filament-binding interface on the Arp2/3 complex is critical for nucleation and branch stability. (PMID: 20404198) Goley ED … Welch MD (Proceedings of the National Academy of Sciences of the United States of America 2010) 3 22
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4
  5. Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. (PMID: 12665801) Gevaert K … Vandekerckhove J (Nature biotechnology 2003) 3 4

Products for ARPC4 Gene

Sources for ARPC4 Gene