Aliases for ARFIP2 Gene

Aliases for ARFIP2 Gene

  • ADP Ribosylation Factor Interacting Protein 2 2 3 5
  • Arfaptin-2 3 4
  • POR1 3 4
  • ADP-Ribosylation Factor-Interacting Protein 2 4
  • Partner Of RAC1 (Arfaptin 2) 3
  • Partner Of RAC1 4
  • Protein POR1 4
  • Arfaptin 2 2

External Ids for ARFIP2 Gene

Previous GeneCards Identifiers for ARFIP2 Gene

  • GC11M006455
  • GC11M006461
  • GC11M006453
  • GC11M006496
  • GC11M006156

Summaries for ARFIP2 Gene

GeneCards Summary for ARFIP2 Gene

ARFIP2 (ADP Ribosylation Factor Interacting Protein 2) is a Protein Coding gene. Diseases associated with ARFIP2 include Gastric Papillary Adenocarcinoma and Signet Ring Cell Adenocarcinoma. Among its related pathways are Cytoskeleton remodeling_Role of PDGFs in cell migration and Vesicle-mediated transport. Gene Ontology (GO) annotations related to this gene include GTP binding and Rac GTPase binding. An important paralog of this gene is ARFIP1.

UniProtKB/Swiss-Prot Summary for ARFIP2 Gene

  • Plays a role in constitutive metalloproteinase (MMP) secretion from the trans Golgi network. May have important functions during vesicle biogenesis at certain cargo subdomains, which could be predominantly utilized by secreted MMPs, such as MMP7 and MMP2 (PubMed:26507660). Participates also in autophagy by regulating the starvation-depdendent trafficking of ATG9A vesicles which deliver the PI4-kinase to the autophagosome initiation site (PubMed:31204568). In addition, plays a role in NF-kappa-B inhibition by interacting with IKBKB and IKBKG (PubMed:26296658).

Gene Wiki entry for ARFIP2 Gene

Additional gene information for ARFIP2 Gene

No data available for Entrez Gene Summary , CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for ARFIP2 Gene

Genomics for ARFIP2 Gene

GeneHancer (GH) Regulatory Elements for ARFIP2 Gene

Promoters and enhancers for ARFIP2 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH11J006479 Promoter/Enhancer 2.2 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 500.7 +0.0 47 3.6 FOXK2 ZBTB40 ZNF217 EP300 SIN3A NRF1 TCF12 POLR2G SP1 GTF2E2 TIMM10B ARFIP2 ENSG00000283977 lnc-TRIM3-1 APBB1 SMPD1
GH11J006925 Promoter/Enhancer 1.9 EPDnew Ensembl ENCODE CraniofacialAtlas 11.6 -445.5 -445496 2.9 NRF1 POLR2A ZBTB11 AFF1 ZBTB26 TRIM28 TARDBP SAP30 HES1 NR3C1 ZNF215 ENSG00000283415 OR2AG2 ARFIP2 ENSG00000254595 piR-48209-091
GH11J006400 Promoter/Enhancer 1.3 Ensembl ENCODE dbSUPER 10.3 +78.1 78109 5.5 ZBTB40 SP1 TEAD4 RFX1 TOE1 RAD51 CBFB USF1 MXI1 GABPA SMPD1 HPX ARFIP2 TIMM10B CAVIN3 APBB1 lnc-HPX-2 RF00017-849
GH11J007019 Promoter/Enhancer 1.9 EPDnew Ensembl ENCODE CraniofacialAtlas 5.8 -538.8 -538830 2.5 NRF1 POLR2G TEAD4 POLR2A ZBTB26 SAP30 TEAD1 HDAC2 MXI1 RBM25 ZNF214 NLRP14 lnc-OR2D2-2 piR-48528 DNHD1 ENSG00000254641 C11orf42 CYB5R2 ARFIP2
GH11J006443 Enhancer 0.8 Ensembl ENCODE 10.6 +37.9 37936 0.7 CTCF REST RAD21 SKI TRIM22 SMC3 JUND SMAD4 RXRA ZFP64 HPX APBB1 ARFIP2 TIMM10B SMPD1 lnc-ARFIP2-3-001
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around ARFIP2 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for ARFIP2

Top Transcription factor binding sites by QIAGEN in the ARFIP2 gene promoter:
  • AREB6
  • ATF
  • Egr-2
  • GR
  • GR-alpha
  • Hlf
  • Pax-4a
  • PPAR-gamma1
  • PPAR-gamma2
  • TGIF

Genomic Locations for ARFIP2 Gene

Genomic Locations for ARFIP2 Gene
chr11:6,474,683-6,481,479
(GRCh38/hg38)
Size:
6,797 bases
Orientation:
Minus strand
chr11:6,495,913-6,502,709
(GRCh37/hg19)
Size:
6,797 bases
Orientation:
Minus strand

Genomic View for ARFIP2 Gene

Genes around ARFIP2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ARFIP2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ARFIP2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ARFIP2 Gene

Proteins for ARFIP2 Gene

  • Protein details for ARFIP2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P53365-ARFP2_HUMAN
    Recommended name:
    Arfaptin-2
    Protein Accession:
    P53365
    Secondary Accessions:
    • B4DX86
    • B4E306
    • D3DQT5

    Protein attributes for ARFIP2 Gene

    Size:
    341 amino acids
    Molecular mass:
    37856 Da
    Quaternary structure:
    • Forms homodimers or heterodimers with ARFIP1 (PubMed:21239483). Interacts with RAC1 (PubMed:11346801). Specifically binds to GTP-bound ARF1 and ARF6, but binds to RAC1.GTP and RAC1.GDP with similar affinities. Interacts with ARL1 (PubMed:11303027, PubMed:22679020, PubMed:26507660). Interacts (via N-terminus) with IKBKB and IKBKG; these interactions inhibit activation of NF-kappa-B (PubMed:26296658).

    Three dimensional structures from OCA and Proteopedia for ARFIP2 Gene

    Alternative splice isoforms for ARFIP2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for ARFIP2 Gene

Post-translational modifications for ARFIP2 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for ARFIP2 Gene

Domains & Families for ARFIP2 Gene

Gene Families for ARFIP2 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for ARFIP2 Gene

Blocks:
  • Arfaptin-like
InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for ARFIP2 Gene

GenScript: Design optimal peptide antigens:
  • Partner of RAC1 (ARFP2_HUMAN)
  • cDNA FLJ51194, highly similar to Arfaptin-2 (B4E306_HUMAN)
genes like me logo Genes that share domains with ARFIP2: view

No data available for Graphical View of Domain Structure and UniProtKB/Swiss-Prot for ARFIP2 Gene

Function for ARFIP2 Gene

Molecular function for ARFIP2 Gene

UniProtKB/Swiss-Prot Function:
Plays a role in constitutive metalloproteinase (MMP) secretion from the trans Golgi network. May have important functions during vesicle biogenesis at certain cargo subdomains, which could be predominantly utilized by secreted MMPs, such as MMP7 and MMP2 (PubMed:26507660). Participates also in autophagy by regulating the starvation-depdendent trafficking of ATG9A vesicles which deliver the PI4-kinase to the autophagosome initiation site (PubMed:31204568). In addition, plays a role in NF-kappa-B inhibition by interacting with IKBKB and IKBKG (PubMed:26296658).

Gene Ontology (GO) - Molecular Function for ARFIP2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 8670882
GO:0005525 GTP binding TAS 8670882
GO:0005543 phospholipid binding IBA 21873635
GO:0019904 protein domain specific binding IEA --
GO:0030742 GTP-dependent protein binding IPI 9312003
genes like me logo Genes that share ontologies with ARFIP2: view
genes like me logo Genes that share phenotypes with ARFIP2: view

Animal Model Products

CRISPR Products

Clone Products

No data available for Enzyme Numbers (IUBMB) , Phenotypes From GWAS Catalog , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for ARFIP2 Gene

Localization for ARFIP2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ARFIP2 Gene

Golgi apparatus. Golgi apparatus, trans-Golgi network membrane.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ARFIP2 gene
Compartment Confidence
plasma membrane 5
cytosol 5
golgi apparatus 5
nucleus 3
cytoskeleton 2
mitochondrion 2
extracellular 1
peroxisome 1
endoplasmic reticulum 1
endosome 1
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Golgi apparatus (2)
  • Nucleoli fibrillar center (2)
  • Nucleoplasm (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for ARFIP2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001726 ruffle IMP 8670882
GO:0005737 cytoplasm TAS,IBA 9312003
GO:0005829 cytosol TAS --
GO:0005886 plasma membrane TAS 9312003
GO:0005938 cell cortex IDA 9312003
genes like me logo Genes that share ontologies with ARFIP2: view

Pathways & Interactions for ARFIP2 Gene

PathCards logo

SuperPathways for ARFIP2 Gene

SuperPathway Contained pathways
1 Vesicle-mediated transport
2 fMLP Pathway
.30
3 GPCR Pathway
.73
4 TCR Signaling (Qiagen)
.39
5 Apoptotic Pathways in Synovial Fibroblasts
.65
genes like me logo Genes that share pathways with ARFIP2: view

Pathways by source for ARFIP2 Gene

2 GeneGo (Thomson Reuters) pathways for ARFIP2 Gene
  • Cytoskeleton remodeling_Role of PDGFs in cell migration
  • G-protein signaling RAC1 in cellular process
5 Qiagen pathways for ARFIP2 Gene
  • Huntington's Disease Pathway
  • Paxillin Interactions
  • PDGF Pathway
  • Rac1 Pathway
  • Rho Family GTPases

SIGNOR curated interactions for ARFIP2 Gene

Other effect:

Gene Ontology (GO) - Biological Process for ARFIP2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006886 intracellular protein transport IBA 21873635
GO:0007264 small GTPase mediated signal transduction TAS 8670882
GO:0030032 lamellipodium assembly TAS 9312003
GO:0030036 actin cytoskeleton organization IMP 9312003
GO:0031529 ruffle organization TAS 9312003
genes like me logo Genes that share ontologies with ARFIP2: view

Drugs & Compounds for ARFIP2 Gene

(1) Drugs for ARFIP2 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Guanosine triphosphate Experimental Pharma 0

(1) Additional Compounds for ARFIP2 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with ARFIP2: view

Transcripts for ARFIP2 Gene

mRNA/cDNA for ARFIP2 Gene

17 REFSEQ mRNAs :
19 NCBI additional mRNA sequence :
10 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for ARFIP2 Gene

ExUns: 1a · 1b · 1c · 1d · 1e · 1f ^ 2 ^ 3a · 3b ^ 4 ^ 5a · 5b · 5c ^ 6a · 6b · 6c · 6d · 6e · 6f · 6g · 6h ^ 7a · 7b · 7c ^ 8a · 8b ^
SP1: - - - - - - - - - - - - -
SP2: - - - - - - - - - -
SP3: - - - - - - - - -
SP4: - - - - - - - - - -
SP5: - - - - - - - - -
SP6: - - - - - - - - - -
SP7: - - - - - - - - - - -
SP8: - - - - - - - - - - -
SP9: - - - - - - - - - - - - -
SP10: - - - - - - -
SP11: - - - - - - - - - -
SP12: - - - - - - - -
SP13: - -
SP14: - - - - - - - - - -
SP15: - -
SP16: - - -
SP17: - -
SP18: - -
SP19: -
SP20:
SP21: - -
SP22: - - -
SP23: -
SP24: - - - - - - - -
SP25:

ExUns: 9 ^ 10a · 10b · 10c ^ 11a · 11b · 11c · 11d · 11e · 11f · 11g ^ 12a · 12b · 12c · 12d
SP1: - -
SP2: - -
SP3: -
SP4: -
SP5:
SP6:
SP7: -
SP8: -
SP9: -
SP10:
SP11:
SP12:
SP13:
SP14:
SP15: -
SP16:
SP17:
SP18:
SP19:
SP20:
SP21:
SP22:
SP23:
SP24:
SP25: - -

Relevant External Links for ARFIP2 Gene

GeneLoc Exon Structure for
ARFIP2

Expression for ARFIP2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for ARFIP2 Gene

Protein differential expression in normal tissues from HIPED for ARFIP2 Gene

This gene is overexpressed in Nasal epithelium (13.4), Skin (12.3), Brain (10.5), and Oral epithelium (6.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for ARFIP2 Gene



Protein tissue co-expression partners for ARFIP2 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for ARFIP2

SOURCE GeneReport for Unigene cluster for ARFIP2 Gene:

Hs.75139

Evidence on tissue expression from TISSUES for ARFIP2 Gene

  • Liver(4.4)
  • Nervous system(4.4)
  • Lung(4.3)
  • Intestine(2.5)
  • Lymph node(2.2)
  • Stomach(2.2)
genes like me logo Genes that share expression patterns with ARFIP2: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for ARFIP2 Gene

Orthologs for ARFIP2 Gene

This gene was present in the common ancestor of animals.

Orthologs for ARFIP2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia ARFIP2 31 30
  • 99.55 (n)
OneToOne
dog
(Canis familiaris)
Mammalia ARFIP2 31 30
  • 96.68 (n)
OneToOne
cow
(Bos Taurus)
Mammalia ARFIP2 31 30
  • 95.5 (n)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia ARFIP2 31
  • 95 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Arfip2 17 31 30
  • 94.43 (n)
rat
(Rattus norvegicus)
Mammalia Arfip2 30
  • 93.84 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia ARFIP2 31
  • 72 (a)
OneToOne
chicken
(Gallus gallus)
Aves ARFIP2 31 30
  • 76.59 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia ARFIP2 31
  • 81 (a)
OneToOne
zebrafish
(Danio rerio)
Actinopterygii arfip2b 31 30
  • 71.03 (n)
OneToMany
arfip2a 31
  • 64 (a)
OneToMany
fruit fly
(Drosophila melanogaster)
Insecta CG17184 31 30
  • 59.3 (n)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP004910 30
  • 55.92 (n)
worm
(Caenorhabditis elegans)
Secernentea F54C8.7 31 30
  • 45.4 (n)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.9407 31
  • 60 (a)
OneToMany
Species where no ortholog for ARFIP2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)

Evolution for ARFIP2 Gene

ENSEMBL:
Gene Tree for ARFIP2 (if available)
TreeFam:
Gene Tree for ARFIP2 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for ARFIP2: view image

Paralogs for ARFIP2 Gene

Paralogs for ARFIP2 Gene

(1) SIMAP similar genes for ARFIP2 Gene using alignment to 3 proteins:

  • ARFP2_HUMAN
  • E9PPY7_HUMAN
  • E9PRV8_HUMAN
genes like me logo Genes that share paralogs with ARFIP2: view

Variants for ARFIP2 Gene

Additional dbSNP identifiers (rs#s) for ARFIP2 Gene

Structural Variations from Database of Genomic Variants (DGV) for ARFIP2 Gene

Variant ID Type Subtype PubMed ID
esv3625266 CNV gain 21293372
nsv972944 CNV duplication 23825009

Variation tolerance for ARFIP2 Gene

Residual Variation Intolerance Score: 11.3% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.52; 11.23% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ARFIP2 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
ARFIP2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for ARFIP2 Gene

Disorders for ARFIP2 Gene

MalaCards: The human disease database

(2) MalaCards diseases for ARFIP2 Gene - From: DISEASES

Disorder Aliases PubMed IDs
gastric papillary adenocarcinoma
  • papillary adenocarcinoma of stomach
signet ring cell adenocarcinoma
  • signet ring carcinoma
- elite association - COSMIC cancer census association via MalaCards

Additional Disease Information for ARFIP2

genes like me logo Genes that share disorders with ARFIP2: view

No data available for UniProtKB/Swiss-Prot and Genatlas for ARFIP2 Gene

Publications for ARFIP2 Gene

  1. Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes. (PMID: 9038142) Kanoh H … Exton JH (The Journal of biological chemistry 1997) 2 3 4 54
  2. Identification of a novel Rac1-interacting protein involved in membrane ruffling. (PMID: 8670882) Van Aelst L … Bar-Sagi D (The EMBO journal 1996) 2 3 4 54
  3. Dimer of arfaptin 2 regulates NF-κB signaling by interacting with IKKβ/NEMO and inhibiting IKKβ kinase activity. (PMID: 26296658) You DJ … Hwang JI (Cellular signalling 2015) 3 4 54
  4. Structural basis for membrane binding specificity of the Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin-2 determined by Arl1 GTPase. (PMID: 22679020) Nakamura K … Wakatsuki S (The Journal of biological chemistry 2012) 3 4 54
  5. Phosphorylation of arfaptin 2 at Ser260 by Akt Inhibits PolyQ-huntingtin-induced toxicity by rescuing proteasome impairment. (PMID: 15809304) Rangone H … Humbert S (The Journal of biological chemistry 2005) 3 23 54

Products for ARFIP2 Gene

Sources for ARFIP2 Gene