Aliases for ARFIP1 Gene

Aliases for ARFIP1 Gene

  • ADP Ribosylation Factor Interacting Protein 1 2 3 5
  • Arfaptin-1 3 4
  • ADP-Ribosylation Factor-Interacting Protein 1 4
  • Arfaptin 1 2
  • HSU52521 3

External Ids for ARFIP1 Gene

Previous GeneCards Identifiers for ARFIP1 Gene

  • GC04P154167
  • GC04P154278
  • GC04P154058
  • GC04P153920
  • GC04P153701
  • GC04P149427
  • GC04P152687

Summaries for ARFIP1 Gene

GeneCards Summary for ARFIP1 Gene

ARFIP1 (ADP Ribosylation Factor Interacting Protein 1) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include protein domain specific binding and phosphatidylinositol-4-phosphate binding. An important paralog of this gene is ARFIP2.

UniProtKB/Swiss-Prot Summary for ARFIP1 Gene

  • Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network. Mechanisitically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold. Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs. In turn, ARFs induce fission. Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells.

Gene Wiki entry for ARFIP1 Gene

Additional gene information for ARFIP1 Gene

No data available for Entrez Gene Summary , CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for ARFIP1 Gene

Genomics for ARFIP1 Gene

GeneHancer (GH) Regulatory Elements for ARFIP1 Gene

Promoters and enhancers for ARFIP1 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH04J152778 Promoter/Enhancer 2 EPDnew Ensembl ENCODE CraniofacialAtlas 554.4 +0.1 64 3.6 ZBTB40 ZNF217 EP300 CTCF SIN3A NRF1 TCF12 POLR2G PHF8 TEAD4 ARFIP1 TIGD4 lnc-FHDC1-4 lnc-TMEM154-2 ENSG00000250980 lnc-TIGD4-3
GH04J153216 Promoter/Enhancer 2.1 Ensembl ENCODE CraniofacialAtlas dbSUPER 40.9 +440.3 440265 9.2 EP300 ZSCAN5C ZNF300 POLR2G USF1 FOS ZFX ZNF639 ZIC2 ZBTB10 ARFIP1 TRIM2 MIR4453HG RNU6-1196P ENSG00000201786 TIGD4 MND1 FHDC1 RNF175 lnc-TIGD4-5
GH04J152934 Promoter/Enhancer 2.3 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER 36.4 +157.0 156964 4.3 HNRNPK EP300 CTCF NRF1 ZNF10 POLR2A ZBTB10 CLOCK ZBTB26 REST FHDC1 ENSG00000287642 LOC729870 ARFIP1 MIR4453HG TRIM2
GH04J153344 Promoter/Enhancer 2.2 EPDnew Ensembl ENCODE CraniofacialAtlas 17.8 +565.4 565364 3 CTCF SIN3A POLR2G PHF8 ZFX POLR2A RBM34 GABPA ZBTB26 REST MND1 ARFIP1 MIR4453HG TMEM131L RF00994-787
GH04J152942 Enhancer 0.8 Ensembl ENCODE dbSUPER 36 +164.3 164269 2.8 NR2F2 EGR2 IKZF2 DPF2 ARFIP1 LOC729870 piR-58122 FHDC1
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around ARFIP1 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for ARFIP1

Top Transcription factor binding sites by QIAGEN in the ARFIP1 gene promoter:
  • aMEF-2
  • Chx10
  • FOXD3
  • HFH-1
  • HSF2
  • MEF-2A
  • STAT1
  • STAT1alpha
  • STAT1beta
  • XBP-1

Genomic Locations for ARFIP1 Gene

Genomic Locations for ARFIP1 Gene
chr4:152,779,937-152,918,463
(GRCh38/hg38)
Size:
138,527 bases
Orientation:
Plus strand
chr4:153,701,089-153,839,615
(GRCh37/hg19)
Size:
138,527 bases
Orientation:
Plus strand

Genomic View for ARFIP1 Gene

Genes around ARFIP1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ARFIP1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ARFIP1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ARFIP1 Gene

Proteins for ARFIP1 Gene

  • Protein details for ARFIP1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P53367-ARFP1_HUMAN
    Recommended name:
    Arfaptin-1
    Protein Accession:
    P53367
    Secondary Accessions:
    • B4DS69
    • Q2M2X4
    • Q3SYL4
    • Q9Y2X6

    Protein attributes for ARFIP1 Gene

    Size:
    373 amino acids
    Molecular mass:
    41738 Da
    Quaternary structure:
    • Forms homodimers or heterodimers with ARFIP2 (PubMed:21239483). Interacts with non-myristoylated GTP-bound ARF3, but not to GDP-bound ARF3 (PubMed:10413101). Interacts with ARF1 (PubMed:10413101, PubMed:22981988). Binds with lower affinity to ARF5 and with very little affinity to ARF6 (PubMed:10413101). Interacts with ARL1 (PubMed:22981988, PubMed:21239483).

    Alternative splice isoforms for ARFIP1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for ARFIP1 Gene

Post-translational modifications for ARFIP1 Gene

  • Phosphorylated by PRKD1; phosphorylation delocalizes ARFIP1 from the Golgi and disrupts its ability to inhibit the activity of ADP-ribosylation factor, an important component of the vesicle scission machinery.
  • Ubiquitination at Lys353
  • Modification sites at PhosphoSitePlus

Antibody Products

  • Boster Bio Antibodies for ARFIP1

No data available for DME Specific Peptides for ARFIP1 Gene

Domains & Families for ARFIP1 Gene

Gene Families for ARFIP1 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for ARFIP1 Gene

Blocks:
  • Arfaptin-like
InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for ARFIP1 Gene

GenScript: Design optimal peptide antigens:
  • ADP-ribosylation factor-interacting protein 1 (ARFP1_HUMAN)
  • cDNA FLJ60846, highly similar to Arfaptin-1 (B4DS69_HUMAN)
  • cDNA FLJ53696, highly similar to Arfaptin-1 (B4E273_HUMAN)
  • cDNA FLJ75640, highly similar to Homo sapiens ADP-ribosylation factor interacting protein 1(arfaptin 1) (ARFIP1), mRNA (Q2M2X4_HUMAN)
genes like me logo Genes that share domains with ARFIP1: view

No data available for Graphical View of Domain Structure and UniProtKB/Swiss-Prot for ARFIP1 Gene

Function for ARFIP1 Gene

Molecular function for ARFIP1 Gene

UniProtKB/Swiss-Prot Function:
Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network. Mechanisitically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold. Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs. In turn, ARFs induce fission. Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells.

Phenotypes From GWAS Catalog for ARFIP1 Gene

Gene Ontology (GO) - Molecular Function for ARFIP1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005543 phospholipid binding IBA 21873635
GO:0019904 protein domain specific binding IEA --
GO:0070273 phosphatidylinositol-4-phosphate binding IDA 23695357
genes like me logo Genes that share ontologies with ARFIP1: view
genes like me logo Genes that share phenotypes with ARFIP1: view

Animal Model Products

CRISPR Products

miRNA for ARFIP1 Gene

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ARFIP1

Clone Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for ARFIP1 Gene

Localization for ARFIP1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ARFIP1 Gene

Golgi apparatus. Golgi apparatus, trans-Golgi network membrane.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ARFIP1 gene
Compartment Confidence
cytosol 5
golgi apparatus 5
nucleus 2
plasma membrane 1
extracellular 1
cytoskeleton 1
endoplasmic reticulum 1
endosome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Golgi apparatus (3)
  • Vesicles (3)
  • Nucleoplasm (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for ARFIP1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane IDA 9038142
GO:0005737 cytoplasm IBA 21873635
GO:0005829 cytosol IDA 9038142
GO:0032588 trans-Golgi network membrane IDA 23695357
genes like me logo Genes that share ontologies with ARFIP1: view

Pathways & Interactions for ARFIP1 Gene

PathCards logo

SuperPathways for ARFIP1 Gene

No Data Available

SIGNOR curated interactions for ARFIP1 Gene

Is activated by:

Gene Ontology (GO) - Biological Process for ARFIP1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006886 intracellular protein transport IDA 12606037
GO:0050708 regulation of protein secretion IDA 12606037
genes like me logo Genes that share ontologies with ARFIP1: view

No data available for Pathways by source for ARFIP1 Gene

Drugs & Compounds for ARFIP1 Gene

(1) Drugs for ARFIP1 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Guanosine triphosphate Experimental Pharma 0
genes like me logo Genes that share compounds with ARFIP1: view

Transcripts for ARFIP1 Gene

mRNA/cDNA for ARFIP1 Gene

6 REFSEQ mRNAs :
19 NCBI additional mRNA sequence :
11 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ARFIP1

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for ARFIP1 Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b · 8c ^ 9 ^ 10 ^ 11 ^ 12a · 12b · 12c · 12d ^ 13a · 13b · 13c · 13d · 13e ^ 14 ^ 15 ^
SP1: - - - - - - - - -
SP2: - - - - - - - - - -
SP3: - - - - - - - - - - - -
SP4: - - - - - - - - -
SP5: - - - - - - -
SP6: - - - - - - - -
SP7: - - - - - - -
SP8:
SP9: - - - - - - - - - -
SP10: - -
SP11: -
SP12:
SP13: - - - - - -
SP14: - - - - -

ExUns: 16 ^ 17 ^ 18a · 18b · 18c · 18d
SP1: -
SP2: -
SP3: -
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12: -
SP13:
SP14:

Relevant External Links for ARFIP1 Gene

GeneLoc Exon Structure for
ARFIP1

Expression for ARFIP1 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for ARFIP1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for ARFIP1 Gene

This gene is overexpressed in Nasal epithelium (13.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for ARFIP1 Gene



Protein tissue co-expression partners for ARFIP1 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for ARFIP1

SOURCE GeneReport for Unigene cluster for ARFIP1 Gene:

Hs.416089

mRNA Expression by UniProt/SwissProt for ARFIP1 Gene:

P53367-ARFP1_HUMAN
Tissue specificity: Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart.

Evidence on tissue expression from TISSUES for ARFIP1 Gene

  • Nervous system(4.7)
  • Liver(4.2)
genes like me logo Genes that share expression patterns with ARFIP1: view

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for ARFIP1 Gene

Orthologs for ARFIP1 Gene

This gene was present in the common ancestor of animals.

Orthologs for ARFIP1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia ARFIP1 31 30
  • 100 (n)
OneToOne
dog
(Canis familiaris)
Mammalia ARFIP1 31 30
  • 95.26 (n)
OneToOne
cow
(Bos Taurus)
Mammalia ARFIP1 31 30
  • 94.64 (n)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia ARFIP1 31
  • 93 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Arfip1 30
  • 90.71 (n)
mouse
(Mus musculus)
Mammalia Arfip1 17 31 30
  • 89.81 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia ARFIP1 31
  • 83 (a)
OneToOne
chicken
(Gallus gallus)
Aves ARFIP1 31 30
  • 84.19 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia ARFIP1 31
  • 83 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia arfip1 30
  • 77.78 (n)
Str.19914 30
African clawed frog
(Xenopus laevis)
Amphibia loc60382-prov 30
zebrafish
(Danio rerio)
Actinopterygii arfip1 31 30
  • 68.34 (n)
OneToOne
idibd2077 30
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.1457 30
fruit fly
(Drosophila melanogaster)
Insecta CG17184 31
  • 44 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea F54C8.7 31
  • 29 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.9407 31
  • 58 (a)
OneToMany
Cin.5370 30
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.5370 30
Species where no ortholog for ARFIP1 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for ARFIP1 Gene

ENSEMBL:
Gene Tree for ARFIP1 (if available)
TreeFam:
Gene Tree for ARFIP1 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for ARFIP1: view image

Paralogs for ARFIP1 Gene

Paralogs for ARFIP1 Gene

(1) SIMAP similar genes for ARFIP1 Gene using alignment to 3 proteins:

  • ARFP1_HUMAN
  • B4DS69_HUMAN
  • B4E273_HUMAN
genes like me logo Genes that share paralogs with ARFIP1: view

Variants for ARFIP1 Gene

Additional dbSNP identifiers (rs#s) for ARFIP1 Gene

Structural Variations from Database of Genomic Variants (DGV) for ARFIP1 Gene

Variant ID Type Subtype PubMed ID
esv2763399 CNV gain 21179565
esv3602715 CNV loss 21293372
nsv1136914 CNV deletion 24896259
nsv516652 CNV loss 19592680
nsv520249 CNV loss 19592680
nsv522511 CNV loss 19592680
nsv830117 CNV gain 17160897
nsv980266 CNV duplication 23825009

Variation tolerance for ARFIP1 Gene

Residual Variation Intolerance Score: 32.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.52; 44.07% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ARFIP1 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
ARFIP1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for ARFIP1 Gene

Disorders for ARFIP1 Gene

Additional Disease Information for ARFIP1

No disorders were found for ARFIP1 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for ARFIP1 Gene

Publications for ARFIP1 Gene

  1. Arfaptin 1 forms a complex with ADP-ribosylation factor and inhibits phospholipase D. (PMID: 10413101) Williger BT … Exton JH (FEBS letters 1999) 2 3 4 23 54
  2. Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes. (PMID: 9038142) Kanoh H … Exton JH (The Journal of biological chemistry 1997) 2 3 4 54
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 54
  4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4 54
  5. Arfaptin 1 inhibits ADP-ribosylation factor-dependent matrix metalloproteinase-9 secretion induced by phorbol ester in HT 1080 fibrosarcoma cells. (PMID: 12606037) Ho WT … Williger BT (FEBS letters 2003) 3 23 54

Products for ARFIP1 Gene

Sources for ARFIP1 Gene