This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates... See more...

Aliases for ARF6 Gene

Aliases for ARF6 Gene

  • ADP Ribosylation Factor 6 2 3 5
  • ADP-Ribosylation Factor 6 3 4

External Ids for ARF6 Gene

Previous GeneCards Identifiers for ARF6 Gene

  • GC14P047681
  • GC14P044155
  • GC14P048349
  • GC14P049429
  • GC14P050359
  • GC14P030484

Summaries for ARF6 Gene

Entrez Gene Summary for ARF6 Gene

  • This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008]

GeneCards Summary for ARF6 Gene

ARF6 (ADP Ribosylation Factor 6) is a Protein Coding gene. Diseases associated with ARF6 include Cholera and Dientamoebiasis. Among its related pathways are Immune response Role of DAP12 receptors in NK cells and Ras signaling pathway. Gene Ontology (GO) annotations related to this gene include GTP binding and obsolete signal transducer activity. An important paralog of this gene is ARF1.

UniProtKB/Swiss-Prot Summary for ARF6 Gene

  • GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling (PubMed:11266366, PubMed:21170023, PubMed:16737952, PubMed:7589240, PubMed:18400762). Required for normal completion of mitotic cytokinesis (By similarity). Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers (By similarity). Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension (PubMed:14978216). Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization. Regulates surface levels of adherens junction proteins such as CDH1 (By similarity). Required for NTRK1 sorting to the recycling pathway from early endosomes (By similarity).
  • (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.

Gene Wiki entry for ARF6 Gene

Additional gene information for ARF6 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for ARF6 Gene

Genomics for ARF6 Gene

GeneHancer (GH) Regulatory Elements for ARF6 Gene

Promoters and enhancers for ARF6 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around ARF6 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for ARF6

Top Transcription factor binding sites by QIAGEN in the ARF6 gene promoter:
  • AP-1
  • AREB6
  • ATF-2
  • c-Jun
  • HOXA9
  • Nkx2-5
  • USF-1
  • USF-1:USF-2

Genomic Locations for ARF6 Gene

Genomic Locations for ARF6 Gene
chr14:49,893,018-49,897,054
(GRCh38/hg38)
Size:
4,037 bases
Orientation:
Plus strand
chr14:50,359,736-50,363,772
(GRCh37/hg19)
Size:
4,037 bases
Orientation:
Plus strand

Genomic View for ARF6 Gene

Genes around ARF6 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ARF6 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ARF6 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ARF6 Gene

Proteins for ARF6 Gene

  • Protein details for ARF6 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P62330-ARF6_HUMAN
    Recommended name:
    ADP-ribosylation factor 6
    Protein Accession:
    P62330
    Secondary Accessions:
    • P26438
    • Q6FGZ2

    Protein attributes for ARF6 Gene

    Size:
    175 amino acids
    Molecular mass:
    20082 Da
    Quaternary structure:
    • Interacts (when activated) with GGA1, GGA2 and GGA3; the interaction is required for proper subcellular location of GGA1, GGA2 and GGA3 (PubMed:11950392). Interacts with PIP5K1C (PubMed:12847086). Interacts with USP6 (via Rab-GAP TBC domain) (PubMed:15509780). Interacts with RAB11FIP3 and RAB11FIP4 (PubMed:16148947, PubMed:17030804, PubMed:17628206). Interacts with HERC1 (PubMed:15642342). Interacts with ARHGAP21 (PubMed:15793564). Interacts with ASAP3; the interaction is stabilized by calcium ions (PubMed:16737952, PubMed:20510928). Interacts with NCS1/FREQ at the plasma membrane (PubMed:17555535). Interacts with TBC1D24 (PubMed:20727515). Interacts with ECPAS (PubMed:20682791). Interacts with MICALL1 (PubMed:21951725). Interacts with SPAG9 homodimers, forming heterotetramers (PubMed:19644450). Interacts with CYTH3 (PubMed:23940353). Interacts with ASAP2 (By similarity). Interacts with UACA (By similarity). Interacts with KIF23, forming heterodimers and heterotetramers (By similarity). Interacts with C9orf72 (By similarity).
    • (Microbial infection) Interacts with the V.cholerae enterotoxin subunit A1; this causes a conformation change so that the toxin can bind NAD and catalyze the ADP-ribosylation of Gs alpha.
    • (Microbial infection) Interacts with EspG from enteropathogenic E.coli.
    • (Microbial infection) Identified in a complex with RAB1A and EspG from enteropathogenic E.coli.

    Three dimensional structures from OCA and Proteopedia for ARF6 Gene

neXtProt entry for ARF6 Gene

Post-translational modifications for ARF6 Gene

  • Ubiquitination at Lys58 and Lys69
  • Modification sites at PhosphoSitePlus

Other Protein References for ARF6 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for ARF6 Gene

Domains & Families for ARF6 Gene

Gene Families for ARF6 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for ARF6 Gene

Suggested Antigen Peptide Sequences for ARF6 Gene

GenScript: Design optimal peptide antigens:
  • ADP-ribosylation factor 6 (ARF6_HUMAN)
  • cDNA, FLJ94403, Homo sapiens ADP-ribosylation factor 6 (ARF6), mRNA (Q6FGZ2_HUMAN)
  • ARF6 protein (Q6FH17_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P62330

UniProtKB/Swiss-Prot:

ARF6_HUMAN :
  • Belongs to the small GTPase superfamily. Arf family.
Family:
  • Belongs to the small GTPase superfamily. Arf family.
genes like me logo Genes that share domains with ARF6: view

Function for ARF6 Gene

Molecular function for ARF6 Gene

UniProtKB/Swiss-Prot Function:
GTP-binding protein involved in protein trafficking that regulates endocytic recycling and cytoskeleton remodeling (PubMed:11266366, PubMed:21170023, PubMed:16737952, PubMed:7589240, PubMed:18400762). Required for normal completion of mitotic cytokinesis (By similarity). Plays a role in the reorganization of the actin cytoskeleton and the formation of stress fibers (By similarity). Involved in the regulation of dendritic spine development, contributing to the regulation of dendritic branching and filopodia extension (PubMed:14978216). Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization. Regulates surface levels of adherens junction proteins such as CDH1 (By similarity). Required for NTRK1 sorting to the recycling pathway from early endosomes (By similarity).
UniProtKB/Swiss-Prot Function:
(Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
UniProtKB/Swiss-Prot EnzymeRegulation:
Activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP). Activated by ASAP3. Inactivated by ACAP1 and ACAP2. Activated by NGF via NTRK1 (By similarity).
GENATLAS Biochemistry:
ADP ribosylation factor 6,class III,low molecular weight,widely expressed,involved in vesicular trafficking,activator of PIP5K1A

Phenotypes From GWAS Catalog for ARF6 Gene

Gene Ontology (GO) - Molecular Function for ARF6 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003924 GTPase activity TAS,IEA --
GO:0005515 protein binding IPI 9312003
GO:0005525 GTP binding IBA,TAS 10913182
GO:0031996 thioesterase binding IPI 15509780
GO:0047485 protein N-terminus binding IEA --
genes like me logo Genes that share ontologies with ARF6: view
genes like me logo Genes that share phenotypes with ARF6: view

Animal Models for ARF6 Gene

MGI Knock Outs for ARF6:
  • Arf6 Arf6<tm1Yaka>

Animal Model Products

CRISPR Products

miRNA for ARF6 Gene

miRTarBase miRNAs that target ARF6

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ARF6

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for ARF6 Gene

Localization for ARF6 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ARF6 Gene

Cytoplasm, cytosol. Cell membrane; Lipid-anchor. Endosome membrane; Lipid-anchor. Recycling endosome membrane; Lipid-anchor. Cell projection, filopodium membrane; Lipid-anchor. Cell projection, ruffle. Cleavage furrow. Midbody, Midbody ring. Early endosome membrane; Lipid-anchor. Golgi apparatus, trans-Golgi network membrane; Lipid-anchor. Note=Distributed uniformly on the plasma membrane, as well as throughout the cytoplasm during metaphase. Subsequently concentrated at patches in the equatorial region at the onset of cytokinesis, and becomes distributed in the equatorial region concurrent with cleavage furrow ingression. In late stages of cytokinesis, concentrates at the midbody ring/Flemming body (PubMed:23603394). Recruitment to the midbody ring requires both activation by PSD/EFA6A and interaction with KIF23/MKLP1 (By similarity). After abscission of the intercellular bridge, incorporated into one of the daughter cells as a midbody remnant and localizes to punctate structures beneath the plasma membrane (PubMed:23603394). Recruited to the cell membrane in association with CYTH2 and ARL4C. Colocalizes with DAB2IP at the plasma membrane and endocytic vesicles (By similarity). Myristoylation is required for proper localization to membranes (PubMed:7589240). {ECO:0000250, ECO:0000250 UniProtKB:P62331, ECO:0000269 PubMed:23603394, ECO:0000269 PubMed:7589240}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ARF6 gene
Compartment Confidence
plasma membrane 5
endosome 5
cytosol 4
golgi apparatus 4
cytoskeleton 3
extracellular 2
mitochondrion 2
nucleus 2
endoplasmic reticulum 2
lysosome 2
peroxisome 1

Gene Ontology (GO) - Cellular Components for ARF6 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001726 ruffle IDA,IBA 9312003
GO:0005737 cytoplasm IBA 21873635
GO:0005768 endosome IBA,TAS 10913182
GO:0005769 early endosome IEA --
GO:0005794 Golgi apparatus IEA --
genes like me logo Genes that share ontologies with ARF6: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for ARF6 Gene

Pathways & Interactions for ARF6 Gene

genes like me logo Genes that share pathways with ARF6: view

Pathways by source for ARF6 Gene

1 GeneGo (Thomson Reuters) pathway for ARF6 Gene
  • Immune response CD16 signaling in NK cells
4 Qiagen pathways for ARF6 Gene
  • Cholera Infection
  • Fc-GammaR-Mediated Phagocytosis in Macrophages
  • Paxillin Interactions
  • Remodeling of Adherens Junctions
1 Cell Signaling Technology pathway for ARF6 Gene

SIGNOR curated interactions for ARF6 Gene

Is activated by:

Gene Ontology (GO) - Biological Process for ARF6 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001889 liver development IEA --
GO:0006886 intracellular protein transport IBA 21873635
GO:0007049 cell cycle IEA --
GO:0007155 cell adhesion TAS 10036235
GO:0007399 nervous system development IEA --
genes like me logo Genes that share ontologies with ARF6: view

Drugs & Compounds for ARF6 Gene

(12) Drugs for ARF6 Gene - From: DrugBank, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
5'-Guanosine-Diphosphate-Monothiophosphate Experimental Pharma Target 0
Guanosine diphosphate Experimental Pharma Target 0
Myristic acid Experimental Pharma Target 0
Guanosine triphosphate Experimental Pharma 0

(20) Additional Compounds for ARF6 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with ARF6: view

Transcripts for ARF6 Gene

mRNA/cDNA for ARF6 Gene

1 REFSEQ mRNAs :
14 NCBI additional mRNA sequence :
1 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ARF6

Alternative Splicing Database (ASD) splice patterns (SP) for ARF6 Gene

No ASD Table

Relevant External Links for ARF6 Gene

GeneLoc Exon Structure for
ARF6

Expression for ARF6 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for ARF6 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for ARF6 Gene

This gene is overexpressed in Esophagus - Mucosa (x4.3).

Protein differential expression in normal tissues from HIPED for ARF6 Gene

This gene is overexpressed in Salivary gland (11.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for ARF6 Gene



Protein tissue co-expression partners for ARF6 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for ARF6

SOURCE GeneReport for Unigene cluster for ARF6 Gene:

Hs.525330

mRNA Expression by UniProt/SwissProt for ARF6 Gene:

P62330-ARF6_HUMAN
Tissue specificity: Ubiquitous, with higher levels in heart, substantia nigra, and kidney.

Evidence on tissue expression from TISSUES for ARF6 Gene

  • Nervous system(4.6)
  • Skin(4.5)
  • Blood(4.4)
  • Liver(4.4)
  • Intestine(3.2)
  • Kidney(2.9)
  • Adrenal gland(2.1)
  • Heart(2.1)
  • Muscle(2.1)
genes like me logo Genes that share expression patterns with ARF6: view

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for ARF6 Gene

Orthologs for ARF6 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for ARF6 Gene

Organism Taxonomy Gene Similarity Type Details
oppossum
(Monodelphis domestica)
Mammalia ARF6 31
  • 100 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia ARF6 31
  • 100 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia ARF6 31 30
  • 100 (n)
OneToOne
dog
(Canis familiaris)
Mammalia ARF6 31 30
  • 97.52 (n)
OneToOne
cow
(Bos Taurus)
Mammalia ARF6 31 30
  • 97.52 (n)
OneToOne
rat
(Rattus norvegicus)
Mammalia Arf6 30
  • 96.57 (n)
mouse
(Mus musculus)
Mammalia Arf6 17 31 30
  • 96 (n)
chicken
(Gallus gallus)
Aves ARF6 31 30
  • 88.76 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia ARF6 31
  • 99 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia arf6.2 30
  • 84 (n)
Str.15292 30
African clawed frog
(Xenopus laevis)
Amphibia Xl.963 30
zebrafish
(Danio rerio)
Actinopterygii arf6a 31 30
  • 81.33 (n)
OneToOne
wufj46d09 30
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.1228 30
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP006890 30
  • 79.31 (n)
fruit fly
(Drosophila melanogaster)
Insecta Arf51F 31 32 30
  • 76.63 (n)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea arf-6 31 32 30
  • 73.95 (n)
OneToOne
arl-6 32
  • 52 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes ARF2 31
  • 65 (a)
ManyToMany
ARF1 31
  • 65 (a)
ManyToMany
ARF3 33 30
  • 56.98 (n)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ACL078W 30
  • 63.2 (n)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0C13563g 30
  • 55.81 (n)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.9548 31
  • 81 (a)
OneToOne
Cin.11765 30
bread mold
(Neurospora crassa)
Ascomycetes NCU07173 30
  • 69.9 (n)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes arf6 30
  • 62.45 (n)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.11765 30
Species where no ortholog for ARF6 was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for ARF6 Gene

ENSEMBL:
Gene Tree for ARF6 (if available)
TreeFam:
Gene Tree for ARF6 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for ARF6: view image

Paralogs for ARF6 Gene

(33) SIMAP similar genes for ARF6 Gene using alignment to 2 proteins:

  • ARF6_HUMAN
  • Q6FH17_HUMAN

Pseudogenes.org Pseudogenes for ARF6 Gene

genes like me logo Genes that share paralogs with ARF6: view

Variants for ARF6 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for ARF6 Gene

SNP ID Clinical significance and condition Chr 14 pos Variation AA Info Type
690286 Uncertain Significance: not provided 49,893,923(+) G/A MISSENSE_VARIANT
710032 Benign: not provided 49,893,787(+) C/T SYNONYMOUS_VARIANT
784283 Benign: not provided 49,893,955(+) C/T SYNONYMOUS_VARIANT

Additional dbSNP identifiers (rs#s) for ARF6 Gene

Structural Variations from Database of Genomic Variants (DGV) for ARF6 Gene

Variant ID Type Subtype PubMed ID
esv28926 CNV gain+loss 19812545
nsv952836 CNV duplication 24416366
nsv977466 CNV duplication 23825009

Variation tolerance for ARF6 Gene

Residual Variation Intolerance Score: 38.7% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ARF6 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
ARF6

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ARF6 Gene

Disorders for ARF6 Gene

MalaCards: The human disease database

(2) MalaCards diseases for ARF6 Gene - From: DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
cholera
  • cholera - vibrio cholerae
dientamoebiasis
  • intestinal trichomoniasis
- elite association - COSMIC cancer census association via MalaCards
Search ARF6 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for ARF6

genes like me logo Genes that share disorders with ARF6: view

No data available for UniProtKB/Swiss-Prot and Genatlas for ARF6 Gene

Publications for ARF6 Gene

  1. Molecular characterization of Rab11-FIP3 binding to ARF GTPases. (PMID: 17628206) Schonteich E … Prekeris R (European journal of cell biology 2007) 3 4 23 54
  2. Proteomic identification and functional characterization of a novel ARF6 GTPase-activating protein, ACAP4. (PMID: 16737952) Fang Z … Yao X (Molecular & cellular proteomics : MCP 2006) 3 4 23 54
  3. Rab11-FIP3 and FIP4 interact with Arf6 and the exocyst to control membrane traffic in cytokinesis. (PMID: 16148947) Fielding AB … Gould GW (The EMBO journal 2005) 3 4 23 54
  4. The TBC (Tre-2/Bub2/Cdc16) domain protein TRE17 regulates plasma membrane-endosomal trafficking through activation of Arf6. (PMID: 15509780) Martinu L … Chou MM (Molecular and cellular biology 2004) 3 4 23 54
  5. Sequence, genomic organization, and expression of the human ADP-ribosylation factor 6 (ARF6) gene: a class III ARF. (PMID: 14659046) Lebeda RA … Haun RS (DNA and cell biology 2003) 3 4 23 54

Products for ARF6 Gene

Sources for ARF6 Gene