This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The... See more...

Aliases for ARF4 Gene

Aliases for ARF4 Gene

  • ADP Ribosylation Factor 4 2 3 5
  • ADP-Ribosylation Factor 4 3 4
  • ADP-Ribosylation Factor 2 2 3
  • ARF2 3 4

External Ids for ARF4 Gene

Previous HGNC Symbols for ARF4 Gene

  • ARF2

Previous GeneCards Identifiers for ARF4 Gene

  • GC03M056428
  • GC03M056911
  • GC03M057411
  • GC03M057514
  • GC03M057507
  • GC03M057532

Summaries for ARF4 Gene

Entrez Gene Summary for ARF4 Gene

  • This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq, Jul 2008]

GeneCards Summary for ARF4 Gene

ARF4 (ADP Ribosylation Factor 4) is a Protein Coding gene. Diseases associated with ARF4 include Cholera and Chromosome 17Q21.31 Duplication Syndrome. Among its related pathways are Metabolism of proteins and Neuropathic Pain-Signaling in Dorsal Horn Neurons. Gene Ontology (GO) annotations related to this gene include GTP binding and obsolete signal transducer activity. An important paralog of this gene is ARF5.

UniProtKB/Swiss-Prot Summary for ARF4 Gene

  • GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Gene Wiki entry for ARF4 Gene

Additional gene information for ARF4 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for ARF4 Gene

Genomics for ARF4 Gene

GeneHancer (GH) Regulatory Elements for ARF4 Gene

Promoters and enhancers for ARF4 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH03J057593 Promoter/Enhancer 2.4 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 515.2 +1.1 1123 7 EP300 FOXK2 ZBTB40 ZNF217 CTCF SIN3A NRF1 TCF12 USF1 POLR2G ARF4 ARF4-AS1 lnc-DNAH12-2 DENND6A FLNB FLNB-AS1 TASOR DENND6A-AS1 PDE12 DENND6A-DT
GH03J057555 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE CraniofacialAtlas 15.4 +40.9 40919 4.6 ZBTB40 ZNF217 EP300 SIN3A TCF12 USF1 POLR2G NCOR1 PHF8 TEAD4 ENSG00000287502 PDE12 DENND6A ARF4 TASOR DENND6A-DT APPL1 HESX1 ENSG00000272202 lnc-DNAH12-2
GH03J057690 Promoter/Enhancer 2 EPDnew Ensembl ENCODE CraniofacialAtlas 14.7 -94.3 -94299 3.8 EP300 NRF1 MYC USF1 POLR2G PHF8 ZFX ZIC2 POLR2A ZBTB26 DENND6A DENND6A-DT lnc-ARF4-4 ARF4 IL17RD SLMAP ACOX2 piR-35672-039
GH03J057543 Promoter/Enhancer 1.1 Ensembl ENCODE 15.3 +53.8 53821 0.8 EP300 ZIC2 SAP30 REST RFX1 MXI1 HDAC2 SIN3A ZNF316 SMC3 DNAH12 ARF4 SLMAP RF00026-708 RNU6-483P
GH03J057826 Enhancer 1 Ensembl ENCODE 11.3 -228.3 -228281 3.8 NCOR1 CTCF TCF7 REST ZBTB25 RAD21 SKI TRIM22 TEAD1 TBX3 ARF4 SLMAP DENND6A DENND6A-DT DENND6A-AS1 APPL1 HESX1 DNAH12 FLNB RF00017-4022
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around ARF4 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for ARF4

Top Transcription factor binding sites by QIAGEN in the ARF4 gene promoter:
  • AP-2gamma
  • ATF
  • CREB
  • deltaCREB
  • MAZR
  • MIF-1
  • Pax-4a
  • POU2F1
  • POU2F1a
  • POU2F1b

Genomic Locations for ARF4 Gene

Genomic Locations for ARF4 Gene
chr3:57,571,363-57,598,220
(GRCh38/hg38)
Size:
26,858 bases
Orientation:
Minus strand
chr3:57,557,090-57,583,947
(GRCh37/hg19)
Size:
26,858 bases
Orientation:
Minus strand

Genomic View for ARF4 Gene

Genes around ARF4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ARF4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ARF4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ARF4 Gene

Proteins for ARF4 Gene

  • Protein details for ARF4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P18085-ARF4_HUMAN
    Recommended name:
    ADP-ribosylation factor 4
    Protein Accession:
    P18085
    Secondary Accessions:
    • B2R7J7
    • P21371

    Protein attributes for ARF4 Gene

    Size:
    180 amino acids
    Molecular mass:
    20511 Da
    Quaternary structure:
    No Data Available

    Three dimensional structures from OCA and Proteopedia for ARF4 Gene

neXtProt entry for ARF4 Gene

Post-translational modifications for ARF4 Gene

  • Ubiquitination at Lys142
  • Modification sites at PhosphoSitePlus

Other Protein References for ARF4 Gene

No data available for DME Specific Peptides for ARF4 Gene

Domains & Families for ARF4 Gene

Gene Families for ARF4 Gene

HGNC:
Human Protein Atlas (HPA):
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for ARF4 Gene

Blocks:
  • ADP-ribosylation factor
InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for ARF4 Gene

GenScript: Design optimal peptide antigens:
  • ADP-ribosylation factor 4 (ARF4_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P18085

UniProtKB/Swiss-Prot:

ARF4_HUMAN :
  • Belongs to the small GTPase superfamily. Arf family.
Family:
  • Belongs to the small GTPase superfamily. Arf family.
genes like me logo Genes that share domains with ARF4: view

Function for ARF4 Gene

Molecular function for ARF4 Gene

UniProtKB/Swiss-Prot Function:
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
GENATLAS Biochemistry:
ADP ribosylation factor 4,class II,20kDa,low molecular weight GTP binding protein,a guanine nucleotide dependent protein activator of cholera toxin,widely expressed,except in brain,involved in vesicular trafficking and activation of phospholipase D

Phenotypes From GWAS Catalog for ARF4 Gene

Gene Ontology (GO) - Molecular Function for ARF4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003924 GTPase activity TAS --
GO:0005086 ARF guanyl-nucleotide exchange factor activity TAS --
GO:0005154 epidermal growth factor receptor binding IDA 12446727
GO:0005515 protein binding IPI 17956946
GO:0005525 GTP binding IEA,IDA 12446727
genes like me logo Genes that share ontologies with ARF4: view
genes like me logo Genes that share phenotypes with ARF4: view

Animal Models for ARF4 Gene

MGI Knock Outs for ARF4:
  • Arf4 Arf4<tm1.1Tsdk>
  • Arf4 Arf4<tm1b(KOMP)Wtsi>

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ARF4

Clone Products

  • Addgene plasmids for ARF4

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for ARF4 Gene

Localization for ARF4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ARF4 Gene

Golgi apparatus. Membrane; Lipid-anchor.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ARF4 gene
Compartment Confidence
plasma membrane 5
cytosol 5
golgi apparatus 4
extracellular 2
cytoskeleton 2
mitochondrion 2
endoplasmic reticulum 2
endosome 2
nucleus 1
lysosome 1

Gene Ontology (GO) - Cellular Components for ARF4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IBA,IEA 21873635
GO:0005794 Golgi apparatus IEA --
GO:0005829 cytosol TAS --
GO:0005886 plasma membrane IEA --
GO:0016020 membrane IEA,HDA 19946888
genes like me logo Genes that share ontologies with ARF4: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for ARF4 Gene

Pathways & Interactions for ARF4 Gene

genes like me logo Genes that share pathways with ARF4: view

Pathways by source for ARF4 Gene

2 Qiagen pathways for ARF4 Gene
  • Cholera Infection
  • Paxillin Interactions

Gene Ontology (GO) - Biological Process for ARF4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006471 protein ADP-ribosylation IDA 1899243
GO:0006886 intracellular protein transport IBA 21873635
GO:0006888 ER to Golgi vesicle-mediated transport TAS --
GO:0006890 retrograde vesicle-mediated transport, Golgi to ER IGI,TAS --
GO:0007173 epidermal growth factor receptor signaling pathway IEA --
genes like me logo Genes that share ontologies with ARF4: view

No data available for SIGNOR curated interactions for ARF4 Gene

Drugs & Compounds for ARF4 Gene

(3) Drugs for ARF4 Gene - From: DrugBank, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Guanosine diphosphate Experimental Pharma Target 0
Guanosine triphosphate Experimental Pharma 0
genes like me logo Genes that share compounds with ARF4: view

Transcripts for ARF4 Gene

mRNA/cDNA for ARF4 Gene

1 REFSEQ mRNAs :
9 NCBI additional mRNA sequence :
9 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ARF4

Clone Products

  • Addgene plasmids for ARF4

Alternative Splicing Database (ASD) splice patterns (SP) for ARF4 Gene

ExUns: 1a · 1b · 1c · 1d · 1e ^ 2a · 2b ^ 3 ^ 4 ^ 5a · 5b · 5c · 5d ^ 6 ^ 7a · 7b ^ 8a · 8b · 8c · 8d
SP1: - - -
SP2: - - -
SP3: - -
SP4: - - - - -
SP5: - - - - - -
SP6: -
SP7:

Relevant External Links for ARF4 Gene

GeneLoc Exon Structure for
ARF4

Expression for ARF4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for ARF4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for ARF4 Gene



Protein tissue co-expression partners for ARF4 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for ARF4

SOURCE GeneReport for Unigene cluster for ARF4 Gene:

Hs.652183

Evidence on tissue expression from TISSUES for ARF4 Gene

  • Nervous system(4.9)
  • Skin(4.8)
  • Liver(4.4)
  • Bone marrow(4.3)
  • Lung(4.1)
  • Intestine(3.4)
  • Kidney(3.3)
  • Pancreas(3.3)
  • Stomach(2.7)
  • Blood(2.4)
  • Gall bladder(2.1)
  • Adrenal gland(2)
  • Heart(2)
  • Spleen(2)
  • Thyroid gland(2)
genes like me logo Genes that share expression patterns with ARF4: view

No data available for mRNA differential expression in normal tissues , Protein differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for ARF4 Gene

Orthologs for ARF4 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for ARF4 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia ARF4 31 30
  • 99.63 (n)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia ARF4 31
  • 97 (a)
OneToOne
cow
(Bos Taurus)
Mammalia ARF4 31
  • 97 (a)
OneToOne
dog
(Canis familiaris)
Mammalia ARF4 31 30
  • 95.37 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Arf4 17 31 30
  • 92.59 (n)
rat
(Rattus norvegicus)
Mammalia Arf4 30
  • 91.48 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia ARF4 31
  • 48 (a)
OneToOne
chicken
(Gallus gallus)
Aves ARF4 31 30
  • 83.84 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia ARF4 31
  • 97 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia arf4 30
  • 79.26 (n)
African clawed frog
(Xenopus laevis)
Amphibia LOC398551 30
zebrafish
(Danio rerio)
Actinopterygii arf4a 31 30
  • 76.85 (n)
OneToMany
ARF4 (3 of 3) 31
  • 70 (a)
OneToMany
ARF4 (2 of 3) 31
  • 69 (a)
OneToMany
fruit fly
(Drosophila melanogaster)
Insecta Arf79F 32
  • 82 (a)
Arf102F 31 32 30
  • 69.26 (n)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP002931 30
  • 71.3 (n)
worm
(Caenorhabditis elegans)
Secernentea arf-3 31 32
  • 84 (a)
OneToMany
arf-1 32
  • 81 (a)
arl-6 32
  • 58 (a)
arl-5 32
  • 46 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes ARF1 31
  • 72 (a)
ManyToMany
ARF2 31
  • 71 (a)
ManyToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 74 (a)
OneToMany
Species where no ortholog for ARF4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for ARF4 Gene

ENSEMBL:
Gene Tree for ARF4 (if available)
TreeFam:
Gene Tree for ARF4 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for ARF4: view image

Paralogs for ARF4 Gene

(31) SIMAP similar genes for ARF4 Gene using alignment to 6 proteins:

  • ARF4_HUMAN
  • C9J6P1_HUMAN
  • C9JAK5_HUMAN
  • C9JPM4_HUMAN
  • F8WDB3_HUMAN
  • U3KQF2_HUMAN

Pseudogenes.org Pseudogenes for ARF4 Gene

genes like me logo Genes that share paralogs with ARF4: view

Variants for ARF4 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for ARF4 Gene

SNP ID Clinical significance and condition Chr 03 pos Variation AA Info Type
rs11550597 - p.Val68Ala

Additional dbSNP identifiers (rs#s) for ARF4 Gene

Structural Variations from Database of Genomic Variants (DGV) for ARF4 Gene

Variant ID Type Subtype PubMed ID
nsv1008684 CNV gain 25217958
nsv590368 CNV gain 21841781

Variation tolerance for ARF4 Gene

Residual Variation Intolerance Score: 51% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ARF4 Gene

Human Gene Mutation Database (HGMD)
ARF4
SNPedia medical, phenotypic, and genealogical associations of SNPs for
ARF4

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ARF4 Gene

Disorders for ARF4 Gene

MalaCards: The human disease database

(7) MalaCards diseases for ARF4 Gene - From: DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
cholera
  • cholera - vibrio cholerae
chromosome 17q21.31 duplication syndrome
  • 17q21.31 microduplication syndrome
retinitis pigmentosa 2
  • rp2
retinal disease
  • retinal disorders
bardet-biedl syndrome
  • biedl-bardet syndrome
- elite association - COSMIC cancer census association via MalaCards
Search ARF4 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for ARF4

genes like me logo Genes that share disorders with ARF4: view

No data available for UniProtKB/Swiss-Prot and Genatlas for ARF4 Gene

Publications for ARF4 Gene

  1. Cloning and characterization of the human ADP-ribosylation factor 4 gene. (PMID: 10524252) Lebeda RA … Haun RS (Gene 1999) 3 4 23 54
  2. Selective amplification of an mRNA and related pseudogene for a human ADP-ribosylation factor, a guanine nucleotide-dependent protein activator of cholera toxin. (PMID: 2107548) Monaco L … Vaughan M (Proceedings of the National Academy of Sciences of the United States of America 1990) 2 3 4 54
  3. Identification of ADP-ribosylation factor 4 as a suppressor of N-(4-hydroxyphenyl)retinamide-induced cell death. (PMID: 19041174) Woo IS … Seo HG (Cancer letters 2009) 3 23 54
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 54
  5. Human ADP-ribosylation factor 4 (ARF4) gene. Map position 3p14.1. (PMID: 10099884) Kim HS (Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 1998) 3 23 54

Products for ARF4 Gene

Sources for ARF4 Gene