ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the... See more...

Aliases for ARF3 Gene

Aliases for ARF3 Gene

  • ADP Ribosylation Factor 3 2 3 5
  • Small GTP Binding Protein 2 3
  • ADP-Ribosylation Factor 3 3 4
  • ARF3 5

External Ids for ARF3 Gene

Previous GeneCards Identifiers for ARF3 Gene

  • GC12M049256
  • GC12M049556
  • GC12M049046
  • GC12M047616
  • GC12M049329
  • GC12M046361
  • GC12M049299
  • GC12M048931

Summaries for ARF3 Gene

Entrez Gene Summary for ARF3 Gene

  • ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. The ARF3 gene contains five exons and four introns. [provided by RefSeq, Jul 2008]

GeneCards Summary for ARF3 Gene

ARF3 (ADP Ribosylation Factor 3) is a Protein Coding gene. Diseases associated with ARF3 include Cholera. Among its related pathways are PI Metabolism and Transport to the Golgi and subsequent modification. Gene Ontology (GO) annotations related to this gene include GTP binding and obsolete signal transducer activity. An important paralog of this gene is ARF1.

UniProtKB/Swiss-Prot Summary for ARF3 Gene

  • GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Gene Wiki entry for ARF3 Gene

Additional gene information for ARF3 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for ARF3 Gene

Genomics for ARF3 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for ARF3 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH12J048956 Promoter/Enhancer 2 EPDnew Ensembl ENCODE CraniofacialAtlas 609.8 +0.6 560 3.3 BRCA1 ARHGAP35 CREB1 CTCF TEAD4 PRDM10 ZNF629 RFX1 LEF1 IKZF1 ARF3 ENSG00000272822 lnc-WNT1-6 lnc-WNT10B-1 CCNT1 KANSL2 DDX23 ENSG00000271547 LMBR1L KMT2D
GH12J048937 Promoter 0.7 EPDnew 600.4 +20.5 20515 0.1 IKZF1 PRDM1 CTCF SCRT1 ZFHX2 ZNF335 EGR2 ZC3H8 ARF3 MN297150 MN309463 FKBP11 ENSG00000272822
GH12J048939 Promoter 0.5 EPDnew 600.4 +18.5 18472 0.1 KLF17 SPI1 MN309463 ARF3 MN297150 FKBP11 ENSG00000272822 lnc-WNT10B-1
GH12J048936 Promoter 0.4 EPDnew 600.4 +21.3 21318 0.1 CTCF MN297150 ARF3 MN309463 FKBP11 ENSG00000272822
GH12J048862 Promoter/Enhancer 2.2 EPDnew Ensembl ENCODE CraniofacialAtlas 11.2 +92.9 92862 5.3 SP1 GATAD2A ZNF629 REST TFE3 IKZF1 NFKBIZ KDM1A ZNF692 POLR2A RND1 DDX23 KMT2D ENSG00000271547 CCNT1 KANSL2 SENP1 LMBR1L BCDIN3D PRPF40B
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around ARF3 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for ARF3

Top Transcription factor binding sites by QIAGEN in the ARF3 gene promoter:
  • E2F
  • E2F-1
  • E2F-2
  • HNF-4alpha1
  • HNF-4alpha2
  • SREBP-1a
  • SREBP-1b
  • SREBP-1c

Genomic Locations for ARF3 Gene

Genomic Locations for ARF3 Gene
chr12:48,930,252-48,957,526
(GRCh38/hg38)
Size:
27,275 bases
Orientation:
Minus strand
chr12:49,329,506-49,351,334
(GRCh37/hg19)
Size:
21,829 bases
Orientation:
Minus strand

Genomic View for ARF3 Gene

Genes around ARF3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ARF3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ARF3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ARF3 Gene

Proteins for ARF3 Gene

  • Protein details for ARF3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P61204-ARF3_HUMAN
    Recommended name:
    ADP-ribosylation factor 3
    Protein Accession:
    P61204
    Secondary Accessions:
    • A8K6G8
    • B7ZB63
    • P16587

    Protein attributes for ARF3 Gene

    Size:
    181 amino acids
    Molecular mass:
    20601 Da
    Quaternary structure:
    • Interacts with PRKCABP. Interacts with PI4KB and NCS1/FREQ at the Golgi complex.

    Three dimensional structures from OCA and Proteopedia for ARF3 Gene

    Alternative splice isoforms for ARF3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for ARF3 Gene

Post-translational modifications for ARF3 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for ARF3 Gene

Domains & Families for ARF3 Gene

Gene Families for ARF3 Gene

HGNC:
Human Protein Atlas (HPA):
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for ARF3 Gene

Suggested Antigen Peptide Sequences for ARF3 Gene

GenScript: Design optimal peptide antigens:
  • ADP-ribosylation factor 3 (ARF3_HUMAN)
  • cDNA, FLJ79427, highly similar to ADP-ribosylation factor 3 (B7ZB63_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P61204

UniProtKB/Swiss-Prot:

ARF3_HUMAN :
  • Belongs to the small GTPase superfamily. Arf family.
Family:
  • Belongs to the small GTPase superfamily. Arf family.
genes like me logo Genes that share domains with ARF3: view

Function for ARF3 Gene

Molecular function for ARF3 Gene

UniProtKB/Swiss-Prot Function:
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
GENATLAS Biochemistry:
ADP ribosylation factor 3,class I,low molecular weight GTP binding protein,a guanine nucleotide dependent protein activator of cholera toxin,with two transcripts resulting from alternative use of polyadenylation transcripts,(20kDa),widely expressed,especially in brain,Ras superfamily member,involved in vesicular trafficking and activation of phospholipase D

Phenotypes From GWAS Catalog for ARF3 Gene

Gene Ontology (GO) - Molecular Function for ARF3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0003924 GTPase activity TAS 1744102
GO:0005525 GTP binding IEA,IBA 21873635
genes like me logo Genes that share ontologies with ARF3: view
genes like me logo Genes that share phenotypes with ARF3: view

Animal Models for ARF3 Gene

MGI Knock Outs for ARF3:
  • Arf3 Arf3<tm1b(EUCOMM)Hmgu>

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ARF3

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for ARF3 Gene

Localization for ARF3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ARF3 Gene

Golgi apparatus. Cytoplasm, perinuclear region.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ARF3 gene
Compartment Confidence
plasma membrane 4
extracellular 4
cytosol 4
golgi apparatus 4
cytoskeleton 2
mitochondrion 2
nucleus 2
endoplasmic reticulum 2
endosome 2
lysosome 2
peroxisome 1

Gene Ontology (GO) - Cellular Components for ARF3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane TAS --
GO:0005737 cytoplasm IBA 21873635
GO:0005794 Golgi apparatus IEA --
GO:0005886 plasma membrane IBA 21873635
GO:0048471 perinuclear region of cytoplasm IEA --
genes like me logo Genes that share ontologies with ARF3: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for ARF3 Gene

Pathways & Interactions for ARF3 Gene

genes like me logo Genes that share pathways with ARF3: view

Pathways by source for ARF3 Gene

2 Qiagen pathways for ARF3 Gene
  • Cholera Infection
  • Paxillin Interactions

Gene Ontology (GO) - Biological Process for ARF3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006661 phosphatidylinositol biosynthetic process TAS --
GO:0006886 intracellular protein transport IBA 21873635
GO:0006890 retrograde vesicle-mediated transport, Golgi to ER IGI 24768165
GO:0015031 protein transport IEA --
GO:0016192 vesicle-mediated transport IBA 21873635
genes like me logo Genes that share ontologies with ARF3: view

No data available for SIGNOR curated interactions for ARF3 Gene

Drugs & Compounds for ARF3 Gene

(1) Drugs for ARF3 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Guanosine triphosphate Experimental Pharma 0

(4) Additional Compounds for ARF3 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with ARF3: view

Transcripts for ARF3 Gene

mRNA/cDNA for ARF3 Gene

1 REFSEQ mRNAs :
13 NCBI additional mRNA sequence :
10 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ARF3

Alternative Splicing Database (ASD) splice patterns (SP) for ARF3 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4a · 4b · 4c · 4d · 4e · 4f ^ 5 ^ 6 ^ 7a · 7b · 7c ^ 8a · 8b ^ 9 ^ 10a · 10b ^ 11a · 11b · 11c
SP1: - - - - - - - - -
SP2: - - - - - - - - - - -
SP3: - - - - - - - - - - -
SP4: - - - - - -
SP5: - - - - -
SP6: - - - - - - - - - - - - - - - - - -
SP7: -
SP8: - - - - - - - - - - - - - - - - - -
SP9:
SP10: - -

Relevant External Links for ARF3 Gene

GeneLoc Exon Structure for
ARF3

Expression for ARF3 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for ARF3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for ARF3 Gene

This gene is overexpressed in Brain (24.4), Saliva (22.7), and Heart (17.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for ARF3 Gene



Protein tissue co-expression partners for ARF3 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for ARF3

SOURCE GeneReport for Unigene cluster for ARF3 Gene:

Hs.119177

Evidence on tissue expression from TISSUES for ARF3 Gene

  • Nervous system(5)
  • Skin(4.8)
  • Lung(4.8)
  • Kidney(3.7)
  • Intestine(3.5)
  • Pancreas(3.3)
  • Eye(3.2)
  • Liver(3.2)
  • Heart(3.1)
  • Muscle(3.1)
  • Stomach(3)
  • Blood(2.7)
  • Adrenal gland(2.6)
  • Thyroid gland(2.4)
  • Spleen(2.3)
  • Lymph node(2.3)
  • Bone marrow(2.2)
genes like me logo Genes that share expression patterns with ARF3: view

No data available for mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for ARF3 Gene

Orthologs for ARF3 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for ARF3 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia ARF3 30
  • 100 (n)
Dog
(Canis familiaris)
Mammalia ARF1 31
  • 100 (a)
OneToOne
ARF3 30
  • 95.58 (n)
Cow
(Bos Taurus)
Mammalia ARF3 30
  • 95.76 (n)
Mouse
(Mus musculus)
Mammalia Arf3 30 17 31
  • 92.82 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Arf3 30
  • 91.71 (n)
Lizard
(Anolis carolinensis)
Reptilia ARF3 31
  • 100 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia arf3 30
  • 83.79 (n)
African clawed frog
(Xenopus laevis)
Amphibia Xl.6539 30
Zebrafish
(Danio rerio)
Actinopterygii arf3b 31
  • 100 (a)
OneToOne
arf3a 30
  • 81.77 (n)
Fruit Fly
(Drosophila melanogaster)
Insecta Arf79F 31 32
  • 93 (a)
OneToMany
Arf102F 32
  • 81 (a)
Worm
(Caenorhabditis elegans)
Secernentea arf-1.2 31
  • 92 (a)
OneToMany
arf-1 32
  • 92 (a)
arf-3 32
  • 84 (a)
arl-6 32
  • 61 (a)
arl-5 32
  • 48 (a)
arl-query 32
  • 41 (a)
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes ARF1 31
  • 76 (a)
ManyToMany
ARF2 31
  • 75 (a)
ManyToMany
Thale Cress
(Arabidopsis thaliana)
eudicotyledons ARFA1E 30
  • 75.37 (n)
Soybean
(Glycine max)
eudicotyledons Gma.11398 30
Rice
(Oryza sativa)
Liliopsida Os07g0223400 30
  • 76.85 (n)
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 74 (a)
ManyToMany
Species where no ortholog for ARF3 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Oppossum (Monodelphis domestica)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Platypus (Ornithorhynchus anatinus)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for ARF3 Gene

ENSEMBL:
Gene Tree for ARF3 (if available)
TreeFam:
Gene Tree for ARF3 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for ARF3: view image

Paralogs for ARF3 Gene

(33) SIMAP similar genes for ARF3 Gene using alignment to 5 proteins:

  • ARF3_HUMAN
  • B7ZB63_HUMAN
  • F5H0C7_HUMAN
  • F5H1V1_HUMAN
  • F5H6T5_HUMAN

Pseudogenes.org Pseudogenes for ARF3 Gene

genes like me logo Genes that share paralogs with ARF3: view

Variants for ARF3 Gene

Additional dbSNP identifiers (rs#s) for ARF3 Gene

Structural Variations from Database of Genomic Variants (DGV) for ARF3 Gene

Variant ID Type Subtype PubMed ID
nsv832404 CNV gain 17160897

Variation tolerance for ARF3 Gene

Residual Variation Intolerance Score: 37.8% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ARF3 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
ARF3

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for ARF3 Gene

Disorders for ARF3 Gene

MalaCards: The human disease database

(1) MalaCards diseases for ARF3 Gene - From: DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
cholera
  • cholera - vibrio cholerae
- elite association - COSMIC cancer census association via MalaCards
Search ARF3 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for ARF3

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
genes like me logo Genes that share disorders with ARF3: view

No data available for UniProtKB/Swiss-Prot and Genatlas for ARF3 Gene

Publications for ARF3 Gene

  1. Interaction of the PDZ domain of human PICK1 with class I ADP-ribosylation factors. (PMID: 10623590) Takeya R … Sumimoto H (Biochemical and biophysical research communications 2000) 3 4 23
  2. Assignment of human ADP ribosylation factor (ARF) genes ARF1 and ARF3 to chromosomes 1q42 and 12q13, respectively. (PMID: 8661066) Hirai M … Hashimoto K (Genomics 1996) 2 3 23
  3. Isolation and characterization of the human gene for ADP-ribosylation factor 3, a 20-kDa guanine nucleotide-binding protein activator of cholera toxin. (PMID: 1744102) Tsai SC … Vaughan M (The Journal of biological chemistry 1991) 3 4 23
  4. Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study. (PMID: 20628086) Bailey SD … DREAM investigators (Diabetes care 2010) 3 41
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4

Products for ARF3 Gene

  • Signalway Proteins for ARF3

Sources for ARF3 Gene