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Aliases for ARAF Gene

Aliases for ARAF Gene

  • A-Raf Proto-Oncogene, Serine/Threonine Kinase 2 3 5
  • V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog 1 2 3
  • Proto-Oncogene A-Raf-1 3 4
  • Proto-Oncogene Pks 3 4
  • ARAF1 3 4
  • PKS2 3 4
  • V-Raf Murine Sarcoma 3611 Viral Oncogene-Like Protein 3
  • A-Raf Proto-Oncogene Serine/Threonine-Protein Kinase 3
  • Serine/Threonine-Protein Kinase A-Raf 3
  • Ras-Binding Protein DA-Raf 3
  • Proto-Oncogene A-Raf 4
  • Oncogene ARAF1 3
  • EC 2.7.11.1 4
  • A-RAF 3
  • RAFA1 3
  • PKS 4

External Ids for ARAF Gene

Previous HGNC Symbols for ARAF Gene

  • ARAF1

Previous GeneCards Identifiers for ARAF Gene

  • GC0XP047177
  • GC0XP047305
  • GC0XP047429
  • GC0XP045132

Summaries for ARAF Gene

Entrez Gene Summary for ARAF Gene

  • This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]

CIViC summary for ARAF Gene

  • ARAF has recently become increasingly considered for its oncogenic potential. Its potential as a target for informing clinical action was demonstrated by a single case of advanced lung adenocarcinoma harboring an S214C mutation that, when treated with sorafenib, acheived near-complete clinical remission. This finding has brought new focus on ARAF as a marker that should be assayed for in cancer treatment.

GeneCards Summary for ARAF Gene

ARAF (A-Raf Proto-Oncogene, Serine/Threonine Kinase) is a Protein Coding gene. Diseases associated with ARAF include Pallister-Killian Syndrome and Mycobacterium Marinum. Among its related pathways are Focal Adhesion and Signaling by moderate kinase activity BRAF mutants. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is RAF1.

UniProtKB/Swiss-Prot for ARAF Gene

  • Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.

  • Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.

Tocris Summary for ARAF Gene

  • Raf kinases, a family of three serine/threonine kinases, are part of the Ras-MAPK signaling cascade and phosphorylate MEK. Upon growth factor stimulation, Raf-1 (c-Raf) is activated by GTP-bound Ras and recruited to the cell membrane. This activation process is tightly regulated.

Gene Wiki entry for ARAF Gene

Additional gene information for ARAF Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for ARAF Gene

Genomics for ARAF Gene

GeneHancer (GH) Regulatory Elements for ARAF Gene

Promoters and enhancers for ARAF Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH0XJ047559 Promoter/Enhancer 2.3 EPDnew FANTOM5 Ensembl ENCODE 659.4 +1.3 1329 5.4 HDGF PKNOX1 ARNT SIN3A DMAP1 ZNF2 IRF4 YY1 POLR2B ZNF766 ARAF INE1 FTSJ1 ZNF81 NUS1P1 UBA1 UXT CFP CDK16 GC0XP047571
GH0XJ046572 Promoter/Enhancer 2.2 EPDnew FANTOM5 Ensembl ENCODE 11.6 -986.5 -986474 5.2 HDGF PKNOX1 ARID4B SIN3A YY1 GLIS2 ZNF143 ATF7 SP3 GLIS1 CHST7 INE1 SLC9A7 RP2 ARAF UBA1 ZNF674 PIR58897
GH0XJ046836 Promoter/Enhancer 2.2 EPDnew FANTOM5 Ensembl ENCODE 10.2 -722.2 -722153 5.4 PKNOX1 ATF1 ARID4B SIN3A DMAP1 ZNF48 ZNF121 ZNF213 KLF7 FOS RP2 INE1 UBA1 ZNF81 ARAF CDK16 ZNF674 CHST7 GC0XP046897
GH0XJ047481 Promoter/Enhancer 2 EPDnew Ensembl ENCODE 10.4 -77.6 -77568 3.5 ATF1 ZFP64 SIN3A YY1 TCF12 GLIS2 ZNF143 ATF7 DEK SP3 LINC01560 ZNF41 INE1 UBA1 ZNF81 TIMP1 ELK1 FTSJ1 ARAF GC0XP047511
GH0XJ047657 Promoter/Enhancer 2 EPDnew Ensembl ENCODE 10.4 +97.6 97639 2.9 HDGF PKNOX1 FOXA2 ARNT ARID4B SIN3A DMAP1 ZNF2 YY1 POLR2B UXT UXT-AS1 INE1 ENSG00000204620 ZNF81 UBA1 FTSJ1 RBM3 ZNF182 RBM10
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around ARAF on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the ARAF gene promoter:

Genomic Locations for ARAF Gene

Genomic Locations for ARAF Gene
chrX:47,561,100-47,571,921
(GRCh38/hg38)
Size:
10,822 bases
Orientation:
Plus strand
chrX:47,420,516-47,431,320
(GRCh37/hg19)

Genomic View for ARAF Gene

Genes around ARAF on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ARAF Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ARAF Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ARAF Gene

Proteins for ARAF Gene

  • Protein details for ARAF Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P10398-ARAF_HUMAN
    Recommended name:
    Serine/threonine-protein kinase A-Raf
    Protein Accession:
    P10398
    Secondary Accessions:
    • P07557
    • Q5H9B2
    • Q5H9B3

    Protein attributes for ARAF Gene

    Size:
    606 amino acids
    Molecular mass:
    67585 Da
    Cofactor:
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
    Quaternary structure:
    • Interacts with TH1L/NELFD.

    Three dimensional structures from OCA and Proteopedia for ARAF Gene

    Alternative splice isoforms for ARAF Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for ARAF Gene

Post-translational modifications for ARAF Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for ARAF Gene

Domains & Families for ARAF Gene

Gene Families for ARAF Gene

Graphical View of Domain Structure for InterPro Entry

P10398

UniProtKB/Swiss-Prot:

ARAF_HUMAN :
  • Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Family:
  • Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
genes like me logo Genes that share domains with ARAF: view

Function for ARAF Gene

Molecular function for ARAF Gene

UniProtKB/Swiss-Prot Function:
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.
UniProtKB/Swiss-Prot Function:
Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.

Enzyme Numbers (IUBMB) for ARAF Gene

Gene Ontology (GO) - Molecular Function for ARAF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0004672 protein kinase activity IEA,TAS 3029685
GO:0004674 protein serine/threonine kinase activity IEA,IDA 19667065
GO:0004709 MAP kinase kinase kinase activity IBA,IEA --
GO:0005057 obsolete signal transducer activity, downstream of receptor IEA --
genes like me logo Genes that share ontologies with ARAF: view
genes like me logo Genes that share phenotypes with ARAF: view

Animal Models for ARAF Gene

MGI Knock Outs for ARAF:

Animal Model Products

miRNA for ARAF Gene

miRTarBase miRNAs that target ARAF

Clone Products

  • Addgene plasmids for ARAF

No data available for Phenotypes From GWAS Catalog , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for ARAF Gene

Localization for ARAF Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ARAF gene
Compartment Confidence
cytosol 5
mitochondrion 3
nucleus 3
plasma membrane 1
extracellular 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nucleoli fibrillar center (2)
  • Nucleus (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for ARAF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005575 cellular_component ND --
GO:0005622 intracellular IEA --
GO:0005739 mitochondrion IBA,IEA --
GO:0005829 cytosol TAS,IEA --
genes like me logo Genes that share ontologies with ARAF: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for ARAF Gene

Pathways & Interactions for ARAF Gene

SuperPathway Contained pathways
1 RET signaling
2 Common Cytokine Receptor Gamma-Chain Family Signaling Pathways
3 IL-2 Pathway
4 Endometrial cancer
5 Glioma
.52
.47
genes like me logo Genes that share pathways with ARAF: view

SIGNOR curated interactions for ARAF Gene

Activates:
Is activated by:

Gene Ontology (GO) - Biological Process for ARAF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000165 MAPK cascade TAS --
GO:0000186 activation of MAPKK activity IEA --
GO:0006464 cellular protein modification process TAS 3029685
GO:0006468 protein phosphorylation IEA --
GO:0007165 signal transduction IEA --
genes like me logo Genes that share ontologies with ARAF: view

Drugs & Compounds for ARAF Gene

(32) Drugs for ARAF Gene - From: DrugBank, ApexBio, DGIdb, HMDB, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
regorafenib Approved Pharma inhibitor Inhibitor of VEGFR/PDGFR/FGFR/mutant kit/RET/Raf-1, Kinase Inhibitors, Vascular endothelial growth factor (VEGF) and VEGF receptor (VEGFR) inhibitors 0
Sorafenib Approved, Investigational Pharma inhibitors, inhibitor Raf kinases and tyrosine kinases inhibitor, RAF, VEGFR2, and PDGFR-beta inhibitors, Kinase Inhibitors, Vascular endothelial growth factor (VEGF) and VEGF receptor (VEGFR) inhibitors 717
Alpha-D-Fucose Experimental Pharma Target 0
Alpha-L-Arabinose Experimental Pharma Target 0
Beta-D-Fucose Experimental Pharma Target 0

(27) Additional Compounds for ARAF Gene - From: HMDB, Novoseek, and Tocris

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • 5'-Adenylphosphoric acid
  • Adenosine 5'-diphosphate
  • ADENOSINE-5'-diphosphATE
  • H3ADP
  • 5'-Adenylphosphate
Full agonist, Agonist, Partial agonist, Antagonist, Gating inhibitor 58-64-0
Diglycerides Group A
  • 1-Myristoyl-2-eicosadienoyl-sn-glycerol
  • DAG(14:0/20:2)
  • DAG(14:0/20:2N6)
  • DAG(14:0/20:2W6)
  • DAG(34:2)
Diglycerides Group B
  • 1-(9Z-Octadecenoyl)-2-hexadecanoyl-sn-glycerol
  • 1-O-Oleoyl-2-O-palmitoyl-sn-glycerol
  • DG (18:1(9Z)/16:0/0:0)
  • DG(18:1/16:0)
  • Diglyceride
Diglycerides Group C
  • (2R)-2-Hydroxy-3-(pentadecanoyloxy)propyl (11Z,14Z)-icosa-11,14-dienoic acid
  • DAG(15:0/0:0/20:2w6)
  • Diacylglycerol(15:0/0:0/20:2w6)
  • Diacylglycerol(15:0/0:0/20:2)
  • DAG(15:0/0:0/20:2n6)
Diglycerides Group D
  • 1,2-Di-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycerol
  • 1,2-Didocosahexaenoyl-sn-glycerol
  • DAG(22:6/22:6)
  • DAG(22:6N3/22:6N3)
  • DAG(22:6OMEGA3/22:6OMEGA3)

(4) Tocris Compounds for ARAF Gene

Compound Action Cas Number
GW 5074 Potent, selective c-Raf1 kinase inhibitor 220904-83-6
KG 5 PDGFRbeta, B-Raf, c-Raf, FLT3 and KIT inhibitor 877874-85-6
Kobe 0065 H-Ras-cRaf1 interaction inhibitor; inhibits Raf signaling 436133-68-5
ZM 336372 Potent, selective c-Raf inhibitor 208260-29-1

(5) ApexBio Compounds for ARAF Gene

Compound Action Cas Number
AZ 628 Raf kinases,potent and ATP-competitive 878739-06-1
CEP-32496 BRAF(V600E)inhibitor,highly potent 1188910-76-0
GDC-0879 B-Raf inhibitor,potent and selective 905281-76-7
LGX818 RAF inhibitor,potent and selective 1269440-17-6
TAK-632 Pan-RAF inhibitor,potent and selective 1228591-30-7
genes like me logo Genes that share compounds with ARAF: view

Drug Products

Transcripts for ARAF Gene

Unigene Clusters for ARAF Gene

V-raf murine sarcoma 3611 viral oncogene homolog:
Representative Sequences:

Clone Products

  • Addgene plasmids for ARAF

Alternative Splicing Database (ASD) splice patterns (SP) for ARAF Gene

ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7a · 7b · 7c ^ 8a · 8b ^ 9a · 9b · 9c · 9d ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^
SP1: - - - - - - - -
SP2: - - - - - -
SP3: - - - - - - -
SP4: - - - - - -
SP5: - - - -
SP6: - - - - -
SP7: - - - - -
SP8: - -
SP9: -

ExUns: 15 ^ 16 ^ 17 ^ 18
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:

Relevant External Links for ARAF Gene

GeneLoc Exon Structure for
ARAF
ECgene alternative splicing isoforms for
ARAF

Expression for ARAF Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for ARAF Gene

Protein differential expression in normal tissues from HIPED for ARAF Gene

This gene is overexpressed in Peripheral blood mononuclear cells (19.9).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for ARAF Gene



Protein tissue co-expression partners for ARAF Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of ARAF Gene:

ARAF

SOURCE GeneReport for Unigene cluster for ARAF Gene:

Hs.446641

mRNA Expression by UniProt/SwissProt for ARAF Gene:

P10398-ARAF_HUMAN
Tissue specificity: Predominantly in urogenital tissues.

Evidence on tissue expression from TISSUES for ARAF Gene

  • Kidney(4.5)
  • Liver(4.5)
  • Nervous system(4.3)
  • Muscle(3.1)
  • Lung(2.9)
  • Intestine(2.8)
  • Blood(2.3)
  • Spleen(2)
genes like me logo Genes that share expression patterns with ARAF: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for ARAF Gene

Orthologs for ARAF Gene

This gene was present in the common ancestor of animals.

Orthologs for ARAF Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia ARAF 34
  • 100 (a)
OneToOne
dog
(Canis familiaris)
Mammalia ARAF 34 33
  • 93.95 (n)
OneToOne
cow
(Bos Taurus)
Mammalia ARAF 34 33
  • 92.9 (n)
OneToOne
rat
(Rattus norvegicus)
Mammalia Araf 33
  • 90.62 (n)
mouse
(Mus musculus)
Mammalia Araf 16 34 33
  • 90.29 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia ARAF 34
  • 69 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia ARAF 34
  • 68 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia ARAF 34
  • 63 (a)
OneToOne
zebrafish
(Danio rerio)
Actinopterygii araf 34 33
  • 65.62 (n)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta phl 34
  • 35 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea lin-45 34
  • 30 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.10630 34
  • 36 (a)
OneToMany
Species where no ortholog for ARAF was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)

Evolution for ARAF Gene

ENSEMBL:
Gene Tree for ARAF (if available)
TreeFam:
Gene Tree for ARAF (if available)
Aminode:
Evolutionary constrained regions (ECRs) for ARAF: view image

Paralogs for ARAF Gene

Paralogs for ARAF Gene

(4) SIMAP similar genes for ARAF Gene using alignment to 3 proteins:

  • Q96II5_HUMAN
  • B4DMG5_HUMAN
  • ARAF_HUMAN

Pseudogenes.org Pseudogenes for ARAF Gene

genes like me logo Genes that share paralogs with ARAF: view

Variants for ARAF Gene

Sequence variations from dbSNP and Humsavar for ARAF Gene

SNP ID Clin Chr 0X pos Variation AA Info Type
rs1057519786 not-provided, likely-pathogenic, Non-small cell lung cancer, Malignant melanoma of skin, Papillary renal cell carcinoma, sporadic, Adenocarcinoma of lung 47,566,722(+) C/G/T 5_prime_UTR_variant, coding_sequence_variant, genic_downstream_transcript_variant, missense_variant
rs1057519876 likely-pathogenic, Papillary renal cell carcinoma, sporadic, Adenocarcinoma of lung, Malignant melanoma of skin 47,566,721(+) T/G 5_prime_UTR_variant, coding_sequence_variant, genic_downstream_transcript_variant, missense_variant
VAR_040376 A colorectal adenocarcinoma sample p.Gly331Cys
rs1000473726 -- 47,563,174(+) A/T genic_upstream_transcript_variant, intron_variant
rs1000930549 -- 47,572,048(+) C/T downstream_transcript_variant

Variation tolerance for ARAF Gene

Residual Variation Intolerance Score: 31.2% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.14; 23.06% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ARAF Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
ARAF

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for ARAF Gene

Disorders for ARAF Gene

MalaCards: The human disease database

(4) MalaCards diseases for ARAF Gene - From: OMIM, DISEASES, and GeneCards

Disorder Aliases PubMed IDs
pallister-killian syndrome
  • pks
mycobacterium marinum
  • fish tank granuloma
aland island eye disease
  • aied
adenosquamous cell lung carcinoma
  • adenosquamous lung carcinoma
- elite association - COSMIC cancer census association via MalaCards
Search ARAF in MalaCards View complete list of genes associated with diseases

Additional Disease Information for ARAF

genes like me logo Genes that share disorders with ARAF: view

No data available for UniProtKB/Swiss-Prot and Genatlas for ARAF Gene

Publications for ARAF Gene

  1. Identification of TH1 as an interaction partner of A-Raf kinase. (PMID: 11952167) Yin XL … Gu JX (Molecular and cellular biochemistry 2002) 3 4 22 58
  2. The complete sequence and promoter activity of the human A-raf-1 gene (ARAF1). (PMID: 8020955) Lee JE … Rapp UR (Genomics 1994) 3 4 22 58
  3. PRR5L degradation promotes mTORC2-mediated PKC-δ phosphorylation and cell migration downstream of Gα12. (PMID: 22609986) Gan X … Wu D (Nature cell biology 2012) 3 4 58
  4. Heterogeneous nuclear ribonucleoprotein H blocks MST2-mediated apoptosis in cancer cells by regulating A-Raf transcription.