This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs... See more...

Aliases for API5 Gene

Aliases for API5 Gene

  • Apoptosis Inhibitor 5 2 3 4 5
  • AAC-11 2 3 4
  • Fibroblast Growth Factor 2-Interacting Factor 2 2 3
  • Cell Migration-Inducing Gene 8 Protein 3 4
  • Migration-Inducing Protein MIG8 2 3
  • Antiapoptosis Clone 11 Protein 3 4
  • AAC11 2 3
  • FIF 3 4
  • Fibroblast Growth Factor 2-Interacting Factor 4
  • Protein XAGL 4
  • API5-Like 1 2
  • API5L1 2
  • API-5 4
  • API5 5

External Ids for API5 Gene

Previous GeneCards Identifiers for API5 Gene

  • GC11M045153
  • GC11P044016
  • GC11P043372
  • GC11P043297
  • GC11P043290
  • GC11P043043
  • GC11P043333

Summaries for API5 Gene

Entrez Gene Summary for API5 Gene

  • This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

GeneCards Summary for API5 Gene

API5 (Apoptosis Inhibitor 5) is a Protein Coding gene. Diseases associated with API5 include Desmoid Disease, Hereditary and Asbestos-Related Lung Carcinoma. Gene Ontology (GO) annotations related to this gene include fibroblast growth factor binding.

UniProtKB/Swiss-Prot Summary for API5 Gene

  • Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.

Gene Wiki entry for API5 Gene

Additional gene information for API5 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for API5 Gene

Genomics for API5 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for API5 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around API5 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for API5

Top Transcription factor binding sites by QIAGEN in the API5 gene promoter:
  • Bach1
  • c-Myc
  • HNF-1
  • HNF-1A
  • Max
  • Nkx2-5
  • Sox9
  • SRF
  • SRF (504 AA)
  • ZID

Genomic Locations for API5 Gene

Genomic Locations for API5 Gene
chr11:43,311,963-43,344,530
(GRCh38/hg38)
Size:
32,568 bases
Orientation:
Plus strand
chr11:43,333,505-43,366,080
(GRCh37/hg19)
Size:
32,576 bases
Orientation:
Plus strand

Genomic View for API5 Gene

Genes around API5 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
API5 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for API5 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for API5 Gene

Proteins for API5 Gene

  • Protein details for API5 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9BZZ5-API5_HUMAN
    Recommended name:
    Apoptosis inhibitor 5
    Protein Accession:
    Q9BZZ5
    Secondary Accessions:
    • B4DGR0
    • B4DRJ2
    • B4E283
    • D3DR21
    • O15441
    • Q9Y4J7

    Protein attributes for API5 Gene

    Size:
    524 amino acids
    Molecular mass:
    59005 Da
    Quaternary structure:
    • Monomer. Interacts with FGF2 and ACIN1.
    SequenceCaution:
    • Sequence=AAB86528.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; Sequence=AAB86528.1; Type=Frameshift; Evidence={ECO:0000305};
    Miscellaneous:
    • [Isoform 1]: Incomplete sequence.

    Three dimensional structures from OCA and Proteopedia for API5 Gene

    Alternative splice isoforms for API5 Gene

neXtProt entry for API5 Gene

Post-translational modifications for API5 Gene

  • Acetylation at Lys-251 impairs antiapoptotic function.
  • Ubiquitination at Lys84 and Lys51
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for API5 Gene

Domains & Families for API5 Gene

Gene Families for API5 Gene

Protein Domains for API5 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for API5 Gene

GenScript: Design optimal peptide antigens:
  • Protein XAGL (API5_HUMAN)
  • cDNA FLJ52148, highly similar to Apoptosis inhibitor 5 (B4DDR3_HUMAN)
  • cDNA FLJ53886, highly similar to Apoptosis inhibitor 5 (B4DGR0_HUMAN)
  • cDNA FLJ61353, highly similar to Apoptosis inhibitor 5 (B4DRJ2_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9BZZ5

UniProtKB/Swiss-Prot:

API5_HUMAN :
  • Two regions, an N-terminal (aa 96-107) and a C-terminal (aa 274-311) are required for binding FGF2.
  • Belongs to the API5 family.
Domain:
  • Two regions, an N-terminal (aa 96-107) and a C-terminal (aa 274-311) are required for binding FGF2.
Family:
  • Belongs to the API5 family.
genes like me logo Genes that share domains with API5: view

Function for API5 Gene

Molecular function for API5 Gene

UniProtKB/Swiss-Prot Function:
Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
GENATLAS Biochemistry:
antiapoptosis 5,inhibiting apoptosis after growth factor withdrawal,ubiquitously expressed

Phenotypes From GWAS Catalog for API5 Gene

Gene Ontology (GO) - Molecular Function for API5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003723 RNA binding IBA 21873635
GO:0005515 protein binding IPI 19387494
GO:0017134 fibroblast growth factor binding IPI 11075807
genes like me logo Genes that share ontologies with API5: view
genes like me logo Genes that share phenotypes with API5: view

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for API5

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for API5 Gene

Localization for API5 Gene

Subcellular locations from UniProtKB/Swiss-Prot for API5 Gene

Nucleus. Cytoplasm. Note=Mainly nuclear. Can also be cytoplasmic.
[Isoform 3]: Cytoplasm.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for API5 gene
Compartment Confidence
nucleus 5
cytosol 3
plasma membrane 1
extracellular 1
cytoskeleton 1
mitochondrion 1
peroxisome 1
endoplasmic reticulum 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nuclear speckles (4)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for API5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IBA,IDA 11075807
GO:0005681 spliceosomal complex ISS 11555636
GO:0005737 cytoplasm NAS 11075807
GO:0016020 membrane HDA 19946888
GO:0016607 nuclear speck IDA --
genes like me logo Genes that share ontologies with API5: view

Pathways & Interactions for API5 Gene

PathCards logo

SuperPathways for API5 Gene

No Data Available

Gene Ontology (GO) - Biological Process for API5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006915 apoptotic process IEA --
GO:0043066 negative regulation of apoptotic process TAS,NAS 10780674
GO:2000270 negative regulation of fibroblast apoptotic process IEA --
genes like me logo Genes that share ontologies with API5: view

No data available for Pathways by source and SIGNOR curated interactions for API5 Gene

Drugs & Compounds for API5 Gene

No Compound Related Data Available

Transcripts for API5 Gene

mRNA/cDNA for API5 Gene

4 REFSEQ mRNAs :
18 NCBI additional mRNA sequence :
11 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for API5

Alternative Splicing Database (ASD) splice patterns (SP) for API5 Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13 ^ 14 ^ 15a · 15b
SP1: - -
SP2: -
SP3:
SP4: -

Relevant External Links for API5 Gene

GeneLoc Exon Structure for
API5

Expression for API5 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for API5 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for API5 Gene

This gene is overexpressed in Lymph node (10.3) and Peripheral blood mononuclear cells (10.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for API5 Gene



Protein tissue co-expression partners for API5 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for API5

SOURCE GeneReport for Unigene cluster for API5 Gene:

Hs.435771

mRNA Expression by UniProt/SwissProt for API5 Gene:

Q9BZZ5-API5_HUMAN
Tissue specificity: Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in heart, pancreas and placenta. Highly expressed in several cancers. Preferentially expressed in squamous cell carcinoma versus adenocarcinoma in non-small cell lung cancer.

Evidence on tissue expression from TISSUES for API5 Gene

  • Nervous system(5)
  • Liver(4.5)
  • Kidney(2.5)
  • Skin(2.3)
  • Intestine(2.2)
  • Heart(2.2)
  • Spleen(2.1)
  • Lymph node(2.1)
  • Muscle(2.1)
  • Lung(2)
genes like me logo Genes that share expression patterns with API5: view

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for API5 Gene

Orthologs for API5 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for API5 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia API5 30 31
  • 99.87 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia API5 31
  • 96 (a)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia API5 31
  • 96 (a)
OneToOne
Dog
(Canis familiaris)
Mammalia API5 30 31
  • 93.64 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia API5 30 31
  • 93.39 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Api5 30
  • 90.51 (n)
Mouse
(Mus musculus)
Mammalia Api5 30 17 31
  • 89.85 (n)
OneToOne
Chicken
(Gallus gallus)
Aves API5 30 31
  • 81.63 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia API5 31
  • 93 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia api5 30
  • 74.42 (n)
Str.5903 30
African clawed frog
(Xenopus laevis)
Amphibia srnyz021-prov 30
Zebrafish
(Danio rerio)
Actinopterygii api5 30 31
  • 73.05 (n)
OneToOne
srnyz021 30
Rainbow Trout
(Oncorhynchus mykiss)
Actinopterygii Omy.6581 30
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP009645 30
  • 53.79 (n)
Fruit Fly
(Drosophila melanogaster)
Insecta Aac11 30 31 32
  • 52.82 (n)
OneToOne
Thale Cress
(Arabidopsis thaliana)
eudicotyledons AT1G29030 30
  • 49.13 (n)
Rice
(Oryza sativa)
Liliopsida Os02g0313400 30
  • 43.7 (n)
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 49 (a)
OneToOne
Species where no ortholog for API5 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for API5 Gene

ENSEMBL:
Gene Tree for API5 (if available)
TreeFam:
Gene Tree for API5 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for API5: view image

Paralogs for API5 Gene

Pseudogenes.org Pseudogenes for API5 Gene

genes like me logo Genes that share paralogs with API5: view

No data available for Paralogs for API5 Gene

Variants for API5 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for API5 Gene

SNP ID Clinical significance and condition Chr 11 pos Variation AA Info Type
rs2862934 - p.Gly493Ser
rs5743240 - p.Met300Val
VAR_021519 - p.Pro276Ser

Additional dbSNP identifiers (rs#s) for API5 Gene

Structural Variations from Database of Genomic Variants (DGV) for API5 Gene

Variant ID Type Subtype PubMed ID
esv3306211 CNV mobile element insertion 20981092
esv3355802 CNV insertion 20981092
nsv1145518 CNV deletion 24896259
nsv554119 CNV gain 21841781
nsv972004 CNV duplication 23825009

Variation tolerance for API5 Gene

Residual Variation Intolerance Score: 18.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.79; 16.65% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for API5 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
API5

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for API5 Gene

Disorders for API5 Gene

MalaCards: The human disease database

(3) MalaCards diseases for API5 Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
desmoid disease, hereditary
  • desmd
asbestos-related lung carcinoma
lung cancer
  • adenocarcinoma of lung
- elite association - COSMIC cancer census association via MalaCards
Search API5 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for API5

genes like me logo Genes that share disorders with API5: view

No data available for UniProtKB/Swiss-Prot and Genatlas for API5 Gene

Publications for API5 Gene

  1. AAC-11, a novel cDNA that inhibits apoptosis after growth factor withdrawal. (PMID: 9307294) Tewari M … Rubin R (Cancer research 1997) 2 3 4
  2. Helical repeat structure of apoptosis inhibitor 5 reveals protein-protein interaction modules. (PMID: 22334682) Han BG … Lee BI (The Journal of biological chemistry 2012) 3 4
  3. The antiapoptotic protein AAC-11 interacts with and regulates Acinus-mediated DNA fragmentation. (PMID: 19387494) Rigou P … Poyet JL (The EMBO journal 2009) 3 4
  4. A genome-wide study of common SNPs and CNVs in cognitive performance in the CANTAB. (PMID: 19734545) Need AC … Goldstein DB (Human molecular genetics 2009) 3 41
  5. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PMID: 17081983) Olsen JV … Mann M (Cell 2006) 3 4

Products for API5 Gene

Sources for API5 Gene