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Aliases for ANO9 Gene

Aliases for ANO9 Gene

  • Anoctamin 9 2 3 5
  • Transmembrane Protein 16J 2 3 4
  • Tumor Protein P53-Inducible Protein 5 3 4
  • P53-Induced Gene 5 Protein 3 4
  • TMEM16J 3 4
  • TP53I5 3 4
  • PIG5 3 4
  • Tumor Protein P53 Inducible Protein 5 2
  • Anoctamin-9 3

External Ids for ANO9 Gene

Previous HGNC Symbols for ANO9 Gene

  • TP53I5
  • TMEM16J

Previous GeneCards Identifiers for ANO9 Gene

  • GC11M000409
  • GC11M000233
  • GC11M000418
  • GC11M000419
  • GC11M000420
  • GC11M000421
  • GC11M000422
  • GC11M000423
  • GC11M000424
  • GC11M000425

Summaries for ANO9 Gene

Entrez Gene Summary for ANO9 Gene

  • The protein encoded by this gene is a member of the TMEM16 (anoctamin) family of proteins, some of which form integral membrane calcium-activated chloride channels. The function of the encoded protein has yet to be elucidated, although it may have channel-forming abilities and also may have phospholipid scramblase activity. This gene has been observed to be upregulated in stage II and III colorectal cancers. [provided by RefSeq, Dec 2016]

GeneCards Summary for ANO9 Gene

ANO9 (Anoctamin 9) is a Protein Coding gene. Among its related pathways are Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds and Ion channel transport. Gene Ontology (GO) annotations related to this gene include intracellular calcium activated chloride channel activity and phospholipid scramblase activity. An important paralog of this gene is ANO4.

UniProtKB/Swiss-Prot for ANO9 Gene

  • Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide (By similarity). Does not exhibit calcium-activated chloride channel (CaCC) activity. Can inhibit the activity of ANO1.

Additional gene information for ANO9 Gene

No data available for CIViC summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for ANO9 Gene

Genomics for ANO9 Gene

GeneHancer (GH) Regulatory Elements for ANO9 Gene

Promoters and enhancers for ANO9 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH11J000441 Promoter/Enhancer 1.8 EPDnew Ensembl ENCODE dbSUPER 650.7 -0.1 -100 1.3 ELF3 KLF1 TFAP4 SAP130 EBF1 ELF1 DPF2 RELA CTBP1 POLR2A ANO9 RN7SL838P RNH1 HRAS PTDSS2
GH11J000440 Enhancer 0.6 ENCODE 650.7 +1.7 1665 0.2 SMARCA5 CTCF BCOR ZMYM3 ZNF16 REST KLF5 RAD21 ZSCAN21 IKZF1 ANO9 RN7SL838P TMEM80 PHRF1 ENSG00000255158 RNH1 LOC105376506
GH11J000443 Promoter/Enhancer 2.5 EPDnew Ensembl ENCODE dbSUPER 12.1 -5.7 -5735 8.2 MLX ZFP64 DMAP1 IRF4 YY1 ZNF213 ZNF143 ZNF548 SP3 NFYC PTDSS2 RNH1 LRRC56 TSPAN4 SNORA52 PHRF1 ENSG00000255237 ANO9 HRAS RIC8A
GH11J000266 Enhancer 1.7 FANTOM5 Ensembl ENCODE dbSUPER 5.2 +172.0 171959 6.8 HDGF PKNOX1 FOXA2 SMAD1 ARNT IRF4 YY1 POLR2B ZNF766 CBX5 PIR53591 RIC8A TMEM80 BET1L PHRF1 IFITM2 PSMD13 SNORA52 PIDD1 SIRT3
GH11J000303 Promoter/Enhancer 2.7 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 1.4 +129.4 129396 17.8 HDGF PKNOX1 FOXA2 SMAD1 ARNT ARID4B SIN3A YY1 POLR2B ZNF766 IFITM2 IFITM3 ENSG00000254910 IFITM1 TMEM80 PGGHG PIDD1 PHRF1 ENSG00000270030 BET1L
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around ANO9 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the ANO9 gene promoter:
  • PPAR-gamma2
  • PPAR-gamma1
  • AML1a
  • Egr-2
  • HNF-4alpha1
  • HNF-4alpha2
  • Spz1
  • Cdc5
  • AREB6

Genomic Locations for ANO9 Gene

Genomic Locations for ANO9 Gene
24,082 bases
Minus strand
24,082 bases
Minus strand

Genomic View for ANO9 Gene

Genes around ANO9 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ANO9 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ANO9 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ANO9 Gene

Proteins for ANO9 Gene

  • Protein details for ANO9 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Protein Accession:
    Secondary Accessions:
    • B3KUC4
    • B4E134
    • Q8TEN4

    Protein attributes for ANO9 Gene

    782 amino acids
    Molecular mass:
    90333 Da
    Quaternary structure:
    No Data Available
    • The term anoctamin was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.
    • Sequence=BAB84914.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};

    Alternative splice isoforms for ANO9 Gene


neXtProt entry for ANO9 Gene

Post-translational modifications for ANO9 Gene

  • Glycosylation at Asn690, posLast=674674, posLast=652652, and Asn641
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for ANO9 Gene

Domains & Families for ANO9 Gene

Gene Families for ANO9 Gene

Human Protein Atlas (HPA):
  • Predicted membrane proteins
  • Transporters

Protein Domains for ANO9 Gene


Suggested Antigen Peptide Sequences for ANO9 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the anoctamin family.
  • Belongs to the anoctamin family.
genes like me logo Genes that share domains with ANO9: view

Function for ANO9 Gene

Molecular function for ANO9 Gene

UniProtKB/Swiss-Prot Function:
Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide (By similarity). Does not exhibit calcium-activated chloride channel (CaCC) activity. Can inhibit the activity of ANO1.

Phenotypes From GWAS Catalog for ANO9 Gene

Gene Ontology (GO) - Molecular Function for ANO9 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005229 intracellular calcium activated chloride channel activity TAS --
GO:0005515 protein binding IPI 21525870
GO:0017128 phospholipid scramblase activity IEA --
genes like me logo Genes that share ontologies with ANO9: view
genes like me logo Genes that share phenotypes with ANO9: view

Animal Model Products

Clone Products

  • Addgene plasmids for ANO9

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , miRNA , Transcription Factor Targets and HOMER Transcription for ANO9 Gene

Localization for ANO9 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ANO9 Gene

Cell membrane; Multi-pass membrane protein. Note=Shows predominantly an intracellular localization with a weak expression in the cell membrane.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ANO9 gene
Compartment Confidence
plasma membrane 5
peroxisome 2

Subcellular locations from the

Human Protein Atlas (HPA)
  • Golgi apparatus (1)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for ANO9 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005622 intracellular IEA --
GO:0005886 plasma membrane TAS --
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane IEA --
genes like me logo Genes that share ontologies with ANO9: view

Pathways & Interactions for ANO9 Gene

genes like me logo Genes that share pathways with ANO9: view

Pathways by source for ANO9 Gene

Gene Ontology (GO) - Biological Process for ANO9 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006821 chloride transport IMP 22946059
GO:0006869 lipid transport IEA --
GO:0034220 ion transmembrane transport TAS --
GO:0061588 calcium activated phospholipid scrambling IEA --
GO:0061589 calcium activated phosphatidylserine scrambling IEA --
genes like me logo Genes that share ontologies with ANO9: view

No data available for SIGNOR curated interactions for ANO9 Gene

Drugs & Compounds for ANO9 Gene

(1) Drugs for ANO9 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
calcium Approved Nutra 0

(1) Additional Compounds for ANO9 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Chloride ion
  • Chloride(1-)
  • Chlorine anion
  • CL(-)
  • Bertholite
  • Chloor
genes like me logo Genes that share compounds with ANO9: view

Transcripts for ANO9 Gene

Unigene Clusters for ANO9 Gene

Anoctamin 9:
Representative Sequences:

Clone Products

  • Addgene plasmids for ANO9

Alternative Splicing Database (ASD) splice patterns (SP) for ANO9 Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11a · 11b · 11c ^ 12 ^ 13a · 13b ^ 14 ^ 15 ^ 16 ^ 17a · 17b ^ 18a ·
SP1: - - - - -
SP2: - - -
SP3: - - -
SP4: - - -
SP5: -
SP6: -
SP7: -

ExUns: 18b · 18c ^ 19a · 19b ^ 20 ^ 21 ^ 22a · 22b ^ 23 ^ 24a · 24b
SP1: - -
SP2: - -
SP3: - -
SP4: -

Relevant External Links for ANO9 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for ANO9 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for ANO9 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for ANO9 Gene

This gene is overexpressed in Colon - Transverse (x8.4), Small Intestine - Terminal Ileum (x7.4), Skin - Not Sun Exposed (Suprapubic) (x6.6), and Skin - Sun Exposed (Lower leg) (x6.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for ANO9 Gene

NURSA nuclear receptor signaling pathways regulating expression of ANO9 Gene:


SOURCE GeneReport for Unigene cluster for ANO9 Gene:


Evidence on tissue expression from TISSUES for ANO9 Gene

  • Spleen(4.2)
  • Intestine(2)
genes like me logo Genes that share expression patterns with ANO9: view

No data available for Protein differential expression in normal tissues , Protein tissue co-expression partners , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for ANO9 Gene

Orthologs for ANO9 Gene

This gene was present in the common ancestor of animals.

Orthologs for ANO9 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia ANO9 34
  • 98 (a)
(Canis familiaris)
Mammalia ANO9 34 33
  • 83.26 (n)
(Rattus norvegicus)
Mammalia Ano9 33
  • 79.06 (n)
(Mus musculus)
Mammalia Ano9 16 34 33
  • 77.61 (n)
(Ornithorhynchus anatinus)
Mammalia ANO9 34
  • 64 (a)
(Gallus gallus)
Aves ANO9 34 33
  • 67.72 (n)
(Anolis carolinensis)
Reptilia ANO9 34
  • 55 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia LOC100493850 33
  • 63.53 (n)
(Danio rerio)
Actinopterygii LOC559809 33
  • 57.65 (n)
ANO9 (2 of 2) 34
  • 46 (a)
ano9a 34
  • 42 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG6938 34
  • 21 (a)
Species where no ortholog for ANO9 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • cow (Bos Taurus)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for ANO9 Gene

Gene Tree for ANO9 (if available)
Gene Tree for ANO9 (if available)
Evolutionary constrained regions (ECRs) for ANO9: view image

Paralogs for ANO9 Gene

Paralogs for ANO9 Gene

(7) SIMAP similar genes for ANO9 Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with ANO9: view

Variants for ANO9 Gene

Sequence variations from dbSNP and Humsavar for ANO9 Gene

SNP ID Clin Chr 11 pos Variation AA Info Type
rs193920739 uncertain-significance, Malignant tumor of prostate 418,804(-) G/C coding_sequence_variant, genic_downstream_transcript_variant, missense_variant, non_coding_transcript_variant
rs1000258150 -- 434,362(-) C/T genic_upstream_transcript_variant, intron_variant
rs1000271510 -- 425,890(-) G/A genic_downstream_transcript_variant, intron_variant
rs1000529634 -- 434,620(-) G/A genic_upstream_transcript_variant, intron_variant
rs1000720022 -- 420,050(-) G/A 3_prime_UTR_variant, genic_downstream_transcript_variant, intron_variant, non_coding_transcript_variant

Structural Variations from Database of Genomic Variants (DGV) for ANO9 Gene

Variant ID Type Subtype PubMed ID
nsv957843 CNV deletion 24416366
nsv951268 CNV deletion 24416366
nsv8754 CNV gain 18304495
nsv825677 CNV loss 20364138
nsv552852 CNV loss 21841781
nsv552846 CNV gain 21841781
nsv552831 CNV gain 21841781
nsv552830 CNV gain+loss 21841781
nsv552829 CNV gain 21841781
nsv552828 CNV gain 21841781
nsv552827 CNV loss 21841781
nsv552826 CNV gain 21841781
nsv552825 CNV gain+loss 21841781
nsv552776 CNV loss 21841781
nsv552770 CNV gain 21841781
nsv527327 CNV loss 19592680
nsv469923 CNV loss 18288195
nsv467633 CNV loss 19166990
nsv1159790 CNV deletion 26073780
nsv1148385 CNV deletion 26484159
nsv1143667 CNV deletion 24896259
nsv1143666 CNV deletion 24896259
nsv1136120 CNV deletion 24896259
nsv1130652 CNV deletion 24896259
nsv1125519 CNV tandem duplication 24896259
nsv1122577 CNV deletion 24896259
nsv1077764 OTHER inversion 25765185
nsv1070919 CNV deletion 25765185
nsv1069989 CNV deletion 25765185
nsv1069988 CNV deletion 25765185
esv3908 CNV loss 18987735
esv3891907 CNV loss 25118596
esv3547173 CNV deletion 23714750
esv32925 CNV gain 17666407
esv3176 CNV loss 18987735
esv29363 CNV loss 19812545
esv2759794 CNV loss 17122850
esv2476569 CNV deletion 19546169
esv1960258 CNV deletion 18987734
esv1030132 CNV deletion 17803354
esv1003820 CNV deletion 20482838
dgv564n106 OTHER inversion 24896259
dgv182e199 CNV deletion 23128226
dgv1521n54 CNV loss 21841781
dgv1520n54 CNV gain 21841781
dgv1519n54 CNV gain+loss 21841781
dgv1518n54 CNV loss 21841781
dgv1517n54 CNV gain 21841781
dgv1505n54 CNV gain 21841781
dgv1012n100 CNV gain 25217958

Variation tolerance for ANO9 Gene

Residual Variation Intolerance Score: 23.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.12; 61.30% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ANO9 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ANO9 Gene

Disorders for ANO9 Gene

Additional Disease Information for ANO9

Genetic Association Database
Human Genome Epidemiology Navigator
ATLAS of Genetics and Cytogenetics in Oncology and Haematology

No disorders were found for ANO9 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for ANO9 Gene

Publications for ANO9 Gene

  1. Structure and function of TMEM16 proteins (anoctamins). (PMID: 24692353) Pedemonte N … Galietta LJ (Physiological reviews 2014) 2 3 58
  2. The anoctamin family: TMEM16A and TMEM16B as calcium-activated chloride channels. (PMID: 21984732) Scudieri P … Galietta LJ (Experimental physiology 2012) 3 4 58
  3. Anoctamins are a family of Ca2+-activated Cl- channels. (PMID: 22946059) Tian Y … Kunzelmann K (Journal of cell science 2012) 3 4 58
  4. CFTR and TMEM16A are separate but functionally related Cl- channels. (PMID: 22178883) Ousingsawat J … Kunzelmann K (Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2011) 3 4 58
  5. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PMID: 20379614) Rose JE … Uhl GR (Molecular medicine (Cambridge, Mass.) 2010) 3 44 58

Products for ANO9 Gene

  • Addgene plasmids for ANO9

Sources for ANO9 Gene

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