A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC i... See more...

Aliases for ANAPC4 Gene

Aliases for ANAPC4 Gene

  • Anaphase Promoting Complex Subunit 4 2 3 5
  • APC4 2 3 4
  • Anaphase-Promoting Complex Subunit 4 3 4
  • Cyclosome Subunit 4 3 4
  • ANAPC4 5

External Ids for ANAPC4 Gene

Previous GeneCards Identifiers for ANAPC4 Gene

  • GC04P025462
  • GC04P025067
  • GC04P025129
  • GC04P025055
  • GC04P024719

Summaries for ANAPC4 Gene

Entrez Gene Summary for ANAPC4 Gene

  • A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

GeneCards Summary for ANAPC4 Gene

ANAPC4 (Anaphase Promoting Complex Subunit 4) is a Protein Coding gene. Diseases associated with ANAPC4 include Joubert Syndrome 31 and Brugada Syndrome 9. Among its related pathways are Cell cycle_Spindle assembly and chromosome separation and Signal transduction_PKA signaling. Gene Ontology (GO) annotations related to this gene include ubiquitin-protein transferase activity and protein phosphatase binding.

UniProtKB/Swiss-Prot Summary for ANAPC4 Gene

  • Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Gene Wiki entry for ANAPC4 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for ANAPC4 Gene

Genomics for ANAPC4 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for ANAPC4 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH04J025376 Promoter/Enhancer 2 EPDnew Ensembl ENCODE CraniofacialAtlas 644 +0.1 88 3.8 BRCA1 IKZF1 POLR2A BACH1 LARP7 ZIC2 RBFOX2 PATZ1 SMAD5 ZNF740 ANAPC4 HSALNG0033401 HSALNG0033402 SLC34A2
GH04J025405 Promoter 0.4 EPDnew 600.3 +28.4 28389 0.1 SAP130 ANAPC4 LOC105374536 HSALNG0033402 SLC34A2
GH04J025312 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE CraniofacialAtlas 86.4 -64.1 -64063 2.1 BRCA1 SP1 HNRNPL GATAD2A CTCF TEAD4 REST ZNF629 TFE3 SIX5 ZCCHC4 ANAPC4 DHX15 PI4K2B piR-35277
GH04J025158 Promoter/Enhancer 2 EPDnew Ensembl ENCODE CraniofacialAtlas 25.1 -216.6 -216575 3.9 ARHGAP35 SP1 GATAD2A ZNF629 PRDM10 REST IKZF1 POLR2A RCOR2 BACH1 PI4K2B SEPSECS SEPSECS-AS1 ANAPC4
GH04J025233 Promoter/Enhancer 2 EPDnew Ensembl ENCODE CraniofacialAtlas 25.7 -142.3 -142303 3.8 CREB1 HNRNPL ATF7 ZNF629 REST CTCF ZNF692 POLR2A BACH1 LARP7 PI4K2B LOC105374535 lnc-SEPSECS-4 ANAPC4 ZCCHC4 piR-39098-167
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around ANAPC4 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for ANAPC4

Top Transcription factor binding sites by QIAGEN in the ANAPC4 gene promoter:
  • aMEF-2
  • AML1a
  • Ik-3
  • MEF-2A
  • POU2F1
  • POU2F1a
  • POU3F1
  • USF-1
  • USF1

Genomic Locations for ANAPC4 Gene

Genomic Locations for ANAPC4 Gene
chr4:25,377,213-25,418,498
(GRCh38/hg38)
Size:
41,286 bases
Orientation:
Plus strand
chr4:25,378,835-25,420,120
(GRCh37/hg19)
Size:
41,286 bases
Orientation:
Plus strand

Genomic View for ANAPC4 Gene

Genes around ANAPC4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ANAPC4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ANAPC4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ANAPC4 Gene

Proteins for ANAPC4 Gene

  • Protein details for ANAPC4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9UJX5-APC4_HUMAN
    Recommended name:
    Anaphase-promoting complex subunit 4
    Protein Accession:
    Q9UJX5
    Secondary Accessions:
    • A8K8H1
    • E9PCR4
    • Q6PCC6
    • Q9NSH6

    Protein attributes for ANAPC4 Gene

    Size:
    808 amino acids
    Molecular mass:
    92116 Da
    Quaternary structure:
    • The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5 (PubMed:25043029, PubMed:27259151, PubMed:9469815, PubMed:26083744). In the context of the APC/C complex, directly interacts with UBE2S (PubMed:27259151).

    Three dimensional structures from OCA and Proteopedia for ANAPC4 Gene

    Alternative splice isoforms for ANAPC4 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for ANAPC4 Gene

Post-translational modifications for ANAPC4 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for ANAPC4 Gene

Domains & Families for ANAPC4 Gene

Gene Families for ANAPC4 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Suggested Antigen Peptide Sequences for ANAPC4 Gene

GenScript: Design optimal peptide antigens:
  • Cyclosome subunit 4 (APC4_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9UJX5

UniProtKB/Swiss-Prot:

APC4_HUMAN :
  • Belongs to the APC4 family.
Family:
  • Belongs to the APC4 family.
genes like me logo Genes that share domains with ANAPC4: view

Function for ANAPC4 Gene

Molecular function for ANAPC4 Gene

UniProtKB/Swiss-Prot Function:
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Phenotypes From GWAS Catalog for ANAPC4 Gene

Gene Ontology (GO) - Molecular Function for ANAPC4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004842 ubiquitin-protein transferase activity TAS 6180011
GO:0005515 protein binding IEA,IPI 20360068
GO:0019903 protein phosphatase binding IPI 21241890
genes like me logo Genes that share ontologies with ANAPC4: view
genes like me logo Genes that share phenotypes with ANAPC4: view

Animal Models for ANAPC4 Gene

MGI Knock Outs for ANAPC4:
  • Anapc4 Anapc4<tm1b(EUCOMM)Wtsi>

Animal Model Products

CRISPR Products

miRNA for ANAPC4 Gene

miRTarBase miRNAs that target ANAPC4

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ANAPC4

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for ANAPC4 Gene

Localization for ANAPC4 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ANAPC4 gene
Compartment Confidence
nucleus 5
cytosol 4
cytoskeleton 3
mitochondrion 2
extracellular 1
plasma membrane 0
golgi apparatus 0

Subcellular locations from the

Human Protein Atlas (HPA)
  • Intermediate filaments (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for ANAPC4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 21241890
GO:0005654 nucleoplasm TAS --
GO:0005680 anaphase-promoting complex IEA,IDA 16364912
GO:0005829 cytosol TAS --
GO:0034399 nuclear periphery IBA 21873635
genes like me logo Genes that share ontologies with ANAPC4: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for ANAPC4 Gene

Pathways & Interactions for ANAPC4 Gene

PathCards logo

SuperPathways for ANAPC4 Gene

SuperPathway Contained pathways
1 Regulation of activated PAK-2p34 by proteasome mediated degradation
2 APC-Cdc20 mediated degradation of Nek2A
3 Class I MHC mediated antigen processing and presentation
4 Cell Cycle, Mitotic
.60
5 Cellular Senescence (REACTOME)
genes like me logo Genes that share pathways with ANAPC4: view

Pathways by source for ANAPC4 Gene

7 GeneGo (Thomson Reuters) pathways for ANAPC4 Gene
  • Cell cycle Regulation of G1/S transition (part 1)
  • Cell cycle Role of SCF complex in cell cycle regulation
  • Cell cycle_Role of APC in cell cycle regulation
  • Cell cycle_Spindle assembly and chromosome separation
  • Development_TGF-beta receptor signaling
6 Qiagen pathways for ANAPC4 Gene
  • Activation of PKA through GPCR
  • cAMP Pathway
  • CDC42 Pathway
  • Epithelial Adherens Junctions
  • Mitotic Roles of Polo Like Kinases

UniProtKB/Swiss-Prot Q9UJX5-APC4_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

Gene Ontology (GO) - Biological Process for ANAPC4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006511 ubiquitin-dependent protein catabolic process TAS --
GO:0007049 cell cycle IEA --
GO:0016567 protein ubiquitination IEA --
GO:0030071 regulation of mitotic metaphase/anaphase transition IEA --
GO:0031145 anaphase-promoting complex-dependent catabolic process IEA,TAS --
genes like me logo Genes that share ontologies with ANAPC4: view

No data available for SIGNOR curated interactions for ANAPC4 Gene

Drugs & Compounds for ANAPC4 Gene

No Compound Related Data Available

Transcripts for ANAPC4 Gene

mRNA/cDNA for ANAPC4 Gene

2 REFSEQ mRNAs :
11 NCBI additional mRNA sequence :
11 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ANAPC4

Alternative Splicing Database (ASD) splice patterns (SP) for ANAPC4 Gene

ExUns: 1a · 1b ^ 2 ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14a · 14b · 14c ^ 15a · 15b ^ 16 ^ 17 ^ 18 ^ 19 ^
SP1: - -
SP2: - -
SP3: -
SP4: -
SP5:
SP6: -
SP7:
SP8:
SP9: -
SP10:

ExUns: 20 ^ 21a · 21b ^ 22a · 22b ^ 23a · 23b ^ 24 ^ 25 ^ 26a · 26b ^ 27 ^ 28 ^ 29a · 29b · 29c
SP1: - - -
SP2: - - -
SP3:
SP4:
SP5:
SP6:
SP7: -
SP8:
SP9:
SP10:

Relevant External Links for ANAPC4 Gene

GeneLoc Exon Structure for
ANAPC4

Expression for ANAPC4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for ANAPC4 Gene

Protein differential expression in normal tissues from HIPED for ANAPC4 Gene

This gene is overexpressed in Lung (22.0) and Breast (19.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for ANAPC4 Gene



Protein tissue co-expression partners for ANAPC4 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for ANAPC4

SOURCE GeneReport for Unigene cluster for ANAPC4 Gene:

Hs.152173

Evidence on tissue expression from TISSUES for ANAPC4 Gene

  • Nervous system(4.5)
genes like me logo Genes that share expression patterns with ANAPC4: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for ANAPC4 Gene

Orthologs for ANAPC4 Gene

This gene was present in the common ancestor of animals.

Orthologs for ANAPC4 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia ANAPC4 30 31
  • 99.83 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia ANAPC4 30 31
  • 93.23 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia ANAPC4 30 31
  • 92.99 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Anapc4 30
  • 87.81 (n)
Mouse
(Mus musculus)
Mammalia Anapc4 30 17 31
  • 87.77 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia ANAPC4 31
  • 77 (a)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia -- 31
  • 76 (a)
OneToMany
-- 31
  • 46 (a)
OneToMany
Chicken
(Gallus gallus)
Aves ANAPC4 30 31
  • 77.97 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia ANAPC4 31
  • 73 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia anapc4 30
  • 70.09 (n)
Str.3790 30
Zebrafish
(Danio rerio)
Actinopterygii anapc4 30 31
  • 64.74 (n)
OneToOne
wufa95b03 30
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP002345 30
  • 41.52 (n)
Fruit Fly
(Drosophila melanogaster)
Insecta APC4 30 31
  • 40.24 (n)
OneToOne
Species where no ortholog for ANAPC4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for ANAPC4 Gene

ENSEMBL:
Gene Tree for ANAPC4 (if available)
TreeFam:
Gene Tree for ANAPC4 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for ANAPC4: view image

Paralogs for ANAPC4 Gene

No data available for Paralogs for ANAPC4 Gene

Variants for ANAPC4 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for ANAPC4 Gene

SNP ID Clinical significance and condition Chr 04 pos Variation AA Info Type
VAR_035792 A colorectal cancer sample p.Ile155Val
rs11550697 - p.Glu800Gly
rs34811474 - p.Arg465Gln

Additional dbSNP identifiers (rs#s) for ANAPC4 Gene

Structural Variations from Database of Genomic Variants (DGV) for ANAPC4 Gene

Variant ID Type Subtype PubMed ID
esv3599962 CNV loss 21293372
nsv509000 CNV insertion 20534489
nsv997544 CNV gain 25217958

Variation tolerance for ANAPC4 Gene

Residual Variation Intolerance Score: 6.23% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 5.20; 69.80% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ANAPC4 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
ANAPC4

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ANAPC4 Gene

Disorders for ANAPC4 Gene

MalaCards: The human disease database

(2) MalaCards diseases for ANAPC4 Gene - From: DISEASES

Disorder Aliases PubMed IDs
joubert syndrome 31
  • jbts31
brugada syndrome 9
  • brgda9
- elite association - COSMIC cancer census association via MalaCards

Additional Disease Information for ANAPC4

genes like me logo Genes that share disorders with ANAPC4: view

No data available for UniProtKB/Swiss-Prot and Genatlas for ANAPC4 Gene

Publications for ANAPC4 Gene

  1. Identification of a cullin homology region in a subunit of the anaphase-promoting complex. (PMID: 9469815) Yu H … Kirschner MW (Science (New York, N.Y.) 1998) 3 4 23
  2. Atomic structure of the APC/C and its mechanism of protein ubiquitination. (PMID: 26083744) Chang L … Barford D (Nature 2015) 3 4
  3. Molecular architecture and mechanism of the anaphase-promoting complex. (PMID: 25043029) Chang LF … Barford D (Nature 2014) 3 4
  4. Genome-wide association study of anthropometric traits and evidence of interactions with age and study year in Filipino women. (PMID: 20966902) Croteau-Chonka DC … Mohlke KL (Obesity (Silver Spring, Md.) 2011) 3 41
  5. Mechanism of ubiquitin-chain formation by the human anaphase-promoting complex. (PMID: 18485873) Jin L … Rape M (Cell 2008) 3 4

Products for ANAPC4 Gene

Sources for ANAPC4 Gene