A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC i... See more...

Aliases for ANAPC4 Gene

Aliases for ANAPC4 Gene

  • Anaphase Promoting Complex Subunit 4 2 3 5
  • Anaphase-Promoting Complex Subunit 4 3 4
  • Cyclosome Subunit 4 3 4
  • APC4 3 4

External Ids for ANAPC4 Gene

Previous GeneCards Identifiers for ANAPC4 Gene

  • GC04P025462
  • GC04P025067
  • GC04P025129
  • GC04P025055
  • GC04P024719

Summaries for ANAPC4 Gene

Entrez Gene Summary for ANAPC4 Gene

  • A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

GeneCards Summary for ANAPC4 Gene

ANAPC4 (Anaphase Promoting Complex Subunit 4) is a Protein Coding gene. Among its related pathways are Cellular Senescence (REACTOME) and Activation of cAMP-Dependent PKA. Gene Ontology (GO) annotations related to this gene include ubiquitin-protein transferase activity and protein phosphatase binding.

UniProtKB/Swiss-Prot Summary for ANAPC4 Gene

  • Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Gene Wiki entry for ANAPC4 Gene

Additional gene information for ANAPC4 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for ANAPC4 Gene

Genomics for ANAPC4 Gene

GeneHancer (GH) Regulatory Elements for ANAPC4 Gene

Promoters and enhancers for ANAPC4 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH04J025376 Promoter/Enhancer 1.9 EPDnew Ensembl ENCODE CraniofacialAtlas 794 +0.1 88 3.8 ZBTB6 RBPJ POLR2A MYC ELF1 MAX ZNF121 MNT TEAD4 ZNF148 ANAPC4 HSALNG0033401 HSALNG0033402
GH04J025405 Promoter 0.4 EPDnew 750.3 +28.4 28389 0.1 SAP130 ANAPC4 LOC105374536 HSALNG0033402
GH04J025312 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE CraniofacialAtlas 72.7 -64.1 -64063 2.1 ZNF24 SP1 CTCF SIX5 ZBTB6 FOXA1 MLX SMARCE1 RBPJ POLR2A ZCCHC4 ANAPC4 DHX15 PI4K2B piR-35277
GH04J025158 Promoter/Enhancer 2 EPDnew Ensembl ENCODE CraniofacialAtlas 45.7 -216.6 -216575 3.9 ZNF24 SP1 MLX RBPJ POLR2A USF2 NR2F6 ATF1 CREB1 HCFC1 PI4K2B SEPSECS SEPSECS-AS1 ANAPC4 ZCCHC4 DHX15
GH04J024469 Promoter/Enhancer 1.8 EPDnew FANTOM5 ENCODE CraniofacialAtlas 39 -905.6 -905574 4.1 ZNF785 CTCF ZBTB6 USF2 L3MBTL2 ZNF121 BCL6B ZNF473 SP7 ZSCAN21 PPARGC1A ANAPC4 DHX15 ZCCHC4 MIR573 LINC02473 piR-50437-497 lnc-DHX15-1
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around ANAPC4 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the ANAPC4 gene promoter:
  • aMEF-2
  • AML1a
  • Ik-3
  • MEF-2A
  • POU2F1
  • POU2F1a
  • POU3F1
  • USF-1
  • USF1

Genomic Locations for ANAPC4 Gene

Genomic Locations for ANAPC4 Gene
chr4:25,377,213-25,418,498
(GRCh38/hg38)
Size:
41,286 bases
Orientation:
Plus strand
chr4:25,378,835-25,420,120
(GRCh37/hg19)
Size:
41,286 bases
Orientation:
Plus strand

Genomic View for ANAPC4 Gene

Genes around ANAPC4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ANAPC4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ANAPC4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ANAPC4 Gene

Proteins for ANAPC4 Gene

  • Protein details for ANAPC4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9UJX5-APC4_HUMAN
    Recommended name:
    Anaphase-promoting complex subunit 4
    Protein Accession:
    Q9UJX5
    Secondary Accessions:
    • A8K8H1
    • E9PCR4
    • Q6PCC6
    • Q9NSH6

    Protein attributes for ANAPC4 Gene

    Size:
    808 amino acids
    Molecular mass:
    92116 Da
    Quaternary structure:
    • The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5 (PubMed:25043029, PubMed:27259151, PubMed:9469815, PubMed:26083744). In the context of the APC/C complex, directly interacts with UBE2S (PubMed:27259151).

    Three dimensional structures from OCA and Proteopedia for ANAPC4 Gene

    Alternative splice isoforms for ANAPC4 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for ANAPC4 Gene

Post-translational modifications for ANAPC4 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for ANAPC4 Gene

Domains & Families for ANAPC4 Gene

Gene Families for ANAPC4 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Suggested Antigen Peptide Sequences for ANAPC4 Gene

GenScript: Design optimal peptide antigens:
  • Cyclosome subunit 4 (APC4_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9UJX5

UniProtKB/Swiss-Prot:

APC4_HUMAN :
  • Belongs to the APC4 family.
Family:
  • Belongs to the APC4 family.
genes like me logo Genes that share domains with ANAPC4: view

Function for ANAPC4 Gene

Molecular function for ANAPC4 Gene

UniProtKB/Swiss-Prot Function:
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Phenotypes From GWAS Catalog for ANAPC4 Gene

Gene Ontology (GO) - Molecular Function for ANAPC4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004842 ubiquitin-protein transferase activity TAS 6180011
GO:0005515 protein binding IPI,IEA 20360068
GO:0019903 protein phosphatase binding IPI 21241890
GO:0061630 contributes_to ubiquitin protein ligase activity IBA 21873635
genes like me logo Genes that share ontologies with ANAPC4: view
genes like me logo Genes that share phenotypes with ANAPC4: view

Animal Models for ANAPC4 Gene

MGI Knock Outs for ANAPC4:
  • Anapc4 Anapc4<tm1b(EUCOMM)Wtsi>

Animal Model Products

CRISPR Products

miRNA for ANAPC4 Gene

miRTarBase miRNAs that target ANAPC4

Clone Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for ANAPC4 Gene

Localization for ANAPC4 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ANAPC4 gene
Compartment Confidence
nucleus 5
cytosol 4
cytoskeleton 2
mitochondrion 2
plasma membrane 0
golgi apparatus 0

Subcellular locations from the

Human Protein Atlas (HPA)
  • Intermediate filaments (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for ANAPC4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 21241890
GO:0005654 nucleoplasm TAS --
GO:0005680 anaphase-promoting complex IDA,IEA 16364912
GO:0005829 cytosol TAS --
GO:0034399 nuclear periphery IBA 21873635
genes like me logo Genes that share ontologies with ANAPC4: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for ANAPC4 Gene

Pathways & Interactions for ANAPC4 Gene

PathCards logo

SuperPathways for ANAPC4 Gene

SuperPathway Contained pathways
1 CDK-mediated phosphorylation and removal of Cdc6
2 APC-Cdc20 mediated degradation of Nek2A
3 Class I MHC mediated antigen processing and presentation
4 Cell Cycle, Mitotic
.60
5 Cellular Senescence (REACTOME)
genes like me logo Genes that share pathways with ANAPC4: view

UniProtKB/Swiss-Prot Q9UJX5-APC4_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

Gene Ontology (GO) - Biological Process for ANAPC4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006511 ubiquitin-dependent protein catabolic process TAS --
GO:0007049 cell cycle IEA --
GO:0016567 protein ubiquitination IEA --
GO:0030071 regulation of mitotic metaphase/anaphase transition IEA --
GO:0031145 anaphase-promoting complex-dependent catabolic process IEA,TAS --
genes like me logo Genes that share ontologies with ANAPC4: view

No data available for SIGNOR curated interactions for ANAPC4 Gene

Drugs & Compounds for ANAPC4 Gene

No Compound Related Data Available

Transcripts for ANAPC4 Gene

mRNA/cDNA for ANAPC4 Gene

CRISPR Products

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for ANAPC4 Gene

ExUns: 1a · 1b ^ 2 ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14a · 14b · 14c ^ 15a · 15b ^ 16 ^ 17 ^ 18 ^ 19 ^
SP1: - -
SP2: - -
SP3: -
SP4: -
SP5:
SP6: -
SP7:
SP8:
SP9: -
SP10:

ExUns: 20 ^ 21a · 21b ^ 22a · 22b ^ 23a · 23b ^ 24 ^ 25 ^ 26a · 26b ^ 27 ^ 28 ^ 29a · 29b · 29c
SP1: - - -
SP2: - - -
SP3:
SP4:
SP5:
SP6:
SP7: -
SP8:
SP9:
SP10:

Relevant External Links for ANAPC4 Gene

GeneLoc Exon Structure for
ANAPC4
ECgene alternative splicing isoforms for
ANAPC4

Expression for ANAPC4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for ANAPC4 Gene

Protein differential expression in normal tissues from HIPED for ANAPC4 Gene

This gene is overexpressed in Lung (22.0) and Breast (19.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for ANAPC4 Gene



Protein tissue co-expression partners for ANAPC4 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of ANAPC4 Gene:

ANAPC4

SOURCE GeneReport for Unigene cluster for ANAPC4 Gene:

Hs.152173

Evidence on tissue expression from TISSUES for ANAPC4 Gene

  • Nervous system(4.4)
genes like me logo Genes that share expression patterns with ANAPC4: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for ANAPC4 Gene

Orthologs for ANAPC4 Gene

This gene was present in the common ancestor of animals.

Orthologs for ANAPC4 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia ANAPC4 33 32
  • 99.83 (n)
OneToOne
cow
(Bos Taurus)
Mammalia ANAPC4 33 32
  • 93.23 (n)
OneToOne
dog
(Canis familiaris)
Mammalia ANAPC4 33 32
  • 92.99 (n)
OneToOne
rat
(Rattus norvegicus)
Mammalia Anapc4 32
  • 87.81 (n)
mouse
(Mus musculus)
Mammalia Anapc4 17 33 32
  • 87.77 (n)
oppossum
(Monodelphis domestica)
Mammalia ANAPC4 33
  • 77 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 33
  • 76 (a)
OneToMany
-- 33
  • 46 (a)
OneToMany
chicken
(Gallus gallus)
Aves ANAPC4 33 32
  • 77.97 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia ANAPC4 33
  • 73 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia anapc4 32
  • 70.09 (n)
Str.3790 32
zebrafish
(Danio rerio)
Actinopterygii anapc4 33 32
  • 64.74 (n)
OneToOne
wufa95b03 32
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP002345 32
  • 41.52 (n)
fruit fly
(Drosophila melanogaster)
Insecta APC4 33 32
  • 40.24 (n)
OneToOne
Species where no ortholog for ANAPC4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for ANAPC4 Gene

ENSEMBL:
Gene Tree for ANAPC4 (if available)
TreeFam:
Gene Tree for ANAPC4 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for ANAPC4: view image

Paralogs for ANAPC4 Gene

No data available for Paralogs for ANAPC4 Gene

Variants for ANAPC4 Gene

Sequence variations from dbSNP and Humsavar for ANAPC4 Gene

SNP ID Clin Chr 04 pos Variation AA Info Type
VAR_035792 A colorectal cancer sample p.Ile155Val
rs1000030943 -- 25,407,387(+) T/A intron_variant
rs1000086215 -- 25,399,297(+) A/G intron_variant
rs10001307 -- 25,390,361(+) C/A/T genic_upstream_transcript_variant, intron_variant
rs10001352 -- 25,411,352(+) T/G intron_variant

Structural Variations from Database of Genomic Variants (DGV) for ANAPC4 Gene

Variant ID Type Subtype PubMed ID
esv3599962 CNV loss 21293372
nsv509000 CNV insertion 20534489
nsv997544 CNV gain 25217958

Variation tolerance for ANAPC4 Gene

Residual Variation Intolerance Score: 6.23% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 5.20; 69.80% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ANAPC4 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
ANAPC4

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ANAPC4 Gene

Disorders for ANAPC4 Gene

Additional Disease Information for ANAPC4

No disorders were found for ANAPC4 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for ANAPC4 Gene

Publications for ANAPC4 Gene

  1. Identification of a cullin homology region in a subunit of the anaphase-promoting complex. (PMID: 9469815) Yu H … Kirschner MW (Science (New York, N.Y.) 1998) 3 4 23 56
  2. Atomic structure of the APC/C and its mechanism of protein ubiquitination. (PMID: 26083744) Chang L … Barford D (Nature 2015) 3 4 56
  3. Molecular architecture and mechanism of the anaphase-promoting complex. (PMID: 25043029) Chang LF … Barford D (Nature 2014) 3 4 56
  4. Genome-wide association study of anthropometric traits and evidence of interactions with age and study year in Filipino women. (PMID: 20966902) Croteau-Chonka DC … Mohlke KL (Obesity (Silver Spring, Md.) 2011) 3 43 56
  5. Mechanism of ubiquitin-chain formation by the human anaphase-promoting complex. (PMID: 18485873) Jin L … Rape M (Cell 2008) 3 4 56

Products for ANAPC4 Gene

Sources for ANAPC4 Gene