This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene t... See more...

Aliases for ALOX15B Gene

Aliases for ALOX15B Gene

  • Arachidonate 15-Lipoxygenase Type B 2 3 5
  • 15-LOX-2 2 3 4
  • Polyunsaturated Fatty Acid Lipoxygenase ALOX15B 3 4
  • Arachidonate 15-Lipoxygenase, Second Type 2 3
  • Arachidonate 15-Lipoxygenase Type II 3 4
  • Linoleate 13-Lipoxygenase 15-LOb 3 4
  • EC 1.13.11.33 4 50
  • 15-LOX-B 3 4
  • Arachidonate Omega(6) Lipoxygenase 3
  • Arachidonate 15-Lipoxygenase 2 3
  • Arachidonate 15-Lipoxygenase B 4
  • 15-Lipoxygenase 2 4
  • 15S-Lipoxygenase 3
  • EC 1.13.11.- 4
  • EC 1.13.11 50
  • ALOX15B 5

External Ids for ALOX15B Gene

Previous GeneCards Identifiers for ALOX15B Gene

  • GC17P008408
  • GC17P008813
  • GC17P007884
  • GC17P008142
  • GC17P007883
  • GC17P007942

Summaries for ALOX15B Gene

Entrez Gene Summary for ALOX15B Gene

  • This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

GeneCards Summary for ALOX15B Gene

ALOX15B (Arachidonate 15-Lipoxygenase Type B) is a Protein Coding gene. Diseases associated with ALOX15B include Embryoma and Autosomal Recessive Congenital Ichthyosis. Among its related pathways are HETE and HPETE biosynthesis and metabolism and Metabolism. Gene Ontology (GO) annotations related to this gene include calcium ion binding and lipid binding. An important paralog of this gene is ALOXE3.

UniProtKB/Swiss-Prot Summary for ALOX15B Gene

  • [Isoform A]: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators (PubMed:9177185, PubMed:10625675, PubMed:12704195, PubMed:17493578, PubMed:18311922, PubMed:24282679, PubMed:10542053, PubMed:24497644, PubMed:32404334) (Probable). It inserts peroxyl groups at C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing (15S)-hydroperoxyeicosatetraenoate/(15S)-HPETE (PubMed:17493578, PubMed:12704195, PubMed:24282679, PubMed:9177185, PubMed:11956198, PubMed:10625675, PubMed:24497644) (Probable). Also peroxidizes linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE (Probable) (PubMed:10542053, PubMed:27435673). Oxygenates arachidonyl derivatives such as 2-arachidonoylglycerol (2-AG) leading to the production and extracellular release of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G) that acts as a peroxisome proliferator-activated receptor alpha agonist (PubMed:18311922, PubMed:17493578, PubMed:11956198). Has the ability to efficiently class-switch ALOX5 pro-inflammatory mediators into anti-inflammatory intermediates (PubMed:27145229). Participates in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) resolvin D5 ((7S,17S)-diHPDHA), which can actively downregulate the immune response and have anti-aggregation properties with platelets (PubMed:32404334). In addition to free PUFAs hydrolyzed from phospholipids, it directly oxidizes PUFAs esterified to membrane-bound phospholipids (PubMed:27435673). Has no detectable 8S-lipoxygenase activity on arachidonate but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE (Probable). May regulate progression through the cell cycle and cell proliferation (PubMed:12704195, PubMed:11839751). May also regulate cytokine secretion by macrophages and therefore play a role in the immune response (PubMed:18067895). May also regulate macrophage differentiation into proatherogenic foam cells (PubMed:22912809).
  • [Isoform B]: Does not convert arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE.

Gene Wiki entry for ALOX15B Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for ALOX15B Gene

Genomics for ALOX15B Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for ALOX15B Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around ALOX15B on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for ALOX15B

Top Transcription factor binding sites by QIAGEN in the ALOX15B gene promoter:
  • AhR
  • CREB
  • GR
  • GR-alpha
  • GR-beta
  • LCR-F1
  • POU3F1
  • PPAR-gamma1
  • RORalpha2
  • SEF-1 (1)

Genomic Locations for ALOX15B Gene

Latest Assembly
chr17:8,039,034-8,049,134
(GRCh38/hg38)
Size:
10,101 bases
Orientation:
Plus strand

Previous Assembly
chr17:7,942,377-7,952,452
(GRCh37/hg19 by Entrez Gene)
Size:
10,076 bases
Orientation:
Plus strand

chr17:7,942,335-7,952,452
(GRCh37/hg19 by Ensembl)
Size:
10,118 bases
Orientation:
Plus strand

Genomic View for ALOX15B Gene

Genes around ALOX15B on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ALOX15B Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ALOX15B Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ALOX15B Gene

Proteins for ALOX15B Gene

  • Protein details for ALOX15B Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O15296-LX15B_HUMAN
    Recommended name:
    Polyunsaturated fatty acid lipoxygenase ALOX15B
    Protein Accession:
    O15296
    Secondary Accessions:
    • D3DTR2
    • Q8IYQ2
    • Q8TEV3
    • Q8TEV4
    • Q8TEV5
    • Q8TEV6
    • Q9UKM4

    Protein attributes for ALOX15B Gene

    Size:
    676 amino acids
    Molecular mass:
    75857 Da
    Cofactor:
    Name=Fe cation; Xref=ChEBI:CHEBI:24875;
    Quaternary structure:
    No Data Available
    SequenceCaution:
    • Sequence=AAD37786.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for ALOX15B Gene

    Alternative splice isoforms for ALOX15B Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for ALOX15B Gene

Selected DME Specific Peptides for ALOX15B Gene

O15296:
  • NPVLIRRC
  • TCSAKHAAV
  • YPDEHFTE

Post-translational modifications for ALOX15B Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for ALOX15B Gene

Domains & Families for ALOX15B Gene

Gene Families for ALOX15B Gene

HGNC:
IUPHAR :
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins

Protein Domains for ALOX15B Gene

InterPro:
Blocks:
  • Lipoxygenase, LH2 domain
  • Lipoxygenase
  • Mammalian lipoxygenase signature

Suggested Antigen Peptide Sequences for ALOX15B Gene

GenScript: Design optimal peptide antigens:
  • Arachidonate 15-lipoxygenase type II (LX15B_HUMAN)

Graphical View of Domain Structure for InterPro Entry

O15296

UniProtKB/Swiss-Prot:

LX15B_HUMAN :
  • The PLAT domain can bind calcium ions; this promotes association with membranes.
  • Belongs to the lipoxygenase family.
Domain:
  • The PLAT domain can bind calcium ions; this promotes association with membranes.
Family:
  • Belongs to the lipoxygenase family.
genes like me logo Genes that share domains with ALOX15B: view

Function for ALOX15B Gene

Molecular function for ALOX15B Gene

UniProtKB/Swiss-Prot Function:
[Isoform A]: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators (PubMed:9177185, PubMed:10625675, PubMed:12704195, PubMed:17493578, PubMed:18311922, PubMed:24282679, PubMed:10542053, PubMed:24497644, PubMed:32404334) (Probable). It inserts peroxyl groups at C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing (15S)-hydroperoxyeicosatetraenoate/(15S)-HPETE (PubMed:17493578, PubMed:12704195, PubMed:24282679, PubMed:9177185, PubMed:11956198, PubMed:10625675, PubMed:24497644) (Probable). Also peroxidizes linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE (Probable) (PubMed:10542053, PubMed:27435673). Oxygenates arachidonyl derivatives such as 2-arachidonoylglycerol (2-AG) leading to the production and extracellular release of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G) that acts as a peroxisome proliferator-activated receptor alpha agonist (PubMed:18311922, PubMed:17493578, PubMed:11956198). Has the ability to efficiently class-switch ALOX5 pro-inflammatory mediators into anti-inflammatory intermediates (PubMed:27145229). Participates in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) resolvin D5 ((7S,17S)-diHPDHA), which can actively downregulate the immune response and have anti-aggregation properties with platelets (PubMed:32404334). In addition to free PUFAs hydrolyzed from phospholipids, it directly oxidizes PUFAs esterified to membrane-bound phospholipids (PubMed:27435673). Has no detectable 8S-lipoxygenase activity on arachidonate but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE (Probable). May regulate progression through the cell cycle and cell proliferation (PubMed:12704195, PubMed:11839751). May also regulate cytokine secretion by macrophages and therefore play a role in the immune response (PubMed:18067895). May also regulate macrophage differentiation into proatherogenic foam cells (PubMed:22912809).
UniProtKB/Swiss-Prot Function:
[Isoform B]: Does not convert arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate; Xref=Rhea:RHEA:16869, ChEBI:CHEBI:15379, ChEBI:CHEBI:32395, ChEBI:CHEBI:57446; EC=1.13.11.33; Evidence={ECO:0000269|PubMed:10625675, ECO:0000269|PubMed:11956198, ECO:0000269|PubMed:12704195, ECO:0000269|PubMed:17493578, ECO:0000269|PubMed:24282679, ECO:0000269|PubMed:24497644, ECO:0000269|PubMed:9177185, ECO:0000305|PubMed:10542053, ECO:0000305|PubMed:27145229, ECO:0000305|PubMed:27435673};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(9Z,12Z)-octadecadienoate + O2 = 13-hydroperoxy-(9Z,11E)-octadecadienoate; Xref=Rhea:RHEA:48848, ChEBI:CHEBI:15379, ChEBI:CHEBI:30245, ChEBI:CHEBI:90823; Evidence={ECO:0000269|PubMed:27435673, ECO:0000305|PubMed:10542053};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + O2 = (5S)-hydroxy-(15S)-hydroperoxy-(6E,8Z,11Z,13E)-eicosatetraenoate; Xref=Rhea:RHEA:53660, ChEBI:CHEBI:15379, ChEBI:CHEBI:90632, ChEBI:CHEBI:137546; Evidence={ECO:0000269|PubMed:27145229};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = 5-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate; Xref=Rhea:RHEA:48844, ChEBI:CHEBI:15379, ChEBI:CHEBI:32395, ChEBI:CHEBI:90822; Evidence={ECO:0000269|PubMed:10542053};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(5S,6R)-dihydroxy-(7E,9E,11Z,14Z)-eicosatetraenoate + O2 = (5S,6R)-dihydroxy-(15S)-hydroperoxy-(7E,9E,11Z,13E)-eicosatetraenoate; Xref=Rhea:RHEA:53656, ChEBI:CHEBI:15379, ChEBI:CHEBI:137542, ChEBI:CHEBI:137547; Evidence={ECO:0000269|PubMed:27145229};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate + O2 = (5S,15S)-dihydroperoxy-(6E,8Z,11Z,13E)-eicosatetraenoate; Xref=Rhea:RHEA:53652, ChEBI:CHEBI:15379, ChEBI:CHEBI:57450, ChEBI:CHEBI:137543; Evidence={ECO:0000269|PubMed:27145229};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + O2 = 2-[15(S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl]-glycerol; Xref=Rhea:RHEA:53332, ChEBI:CHEBI:15379, ChEBI:CHEBI:52392, ChEBI:CHEBI:137187; Evidence={ECO:0000269|PubMed:11956198};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(8S)-hydroperoxy-(5Z,9E,11Z,14Z)-eicosatetraenoate + O2 = (8S,15S)-dihydroperoxy-(5Z,9E,11Z,13E)-eicosatetraenoate; Xref=Rhea:RHEA:50932, ChEBI:CHEBI:15379, ChEBI:CHEBI:75322, ChEBI:CHEBI:133899; Evidence={ECO:0000305|PubMed:16112079};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-alanine + O2 = N-(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-alanine; Xref=Rhea:RHEA:50184, ChEBI:CHEBI:15379, ChEBI:CHEBI:132071, ChEBI:CHEBI:132077; Evidence={ECO:0000269|PubMed:17493578};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-gamma-aminobutanoate + O2 = N-(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-gamma-aminobutanoate; Xref=Rhea:RHEA:50180, ChEBI:CHEBI:15379, ChEBI:CHEBI:132072, ChEBI:CHEBI:132078; Evidence={ECO:0000269|PubMed:17493578};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine + O2 = N-(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-glycine; Xref=Rhea:RHEA:50188, ChEBI:CHEBI:15379, ChEBI:CHEBI:59002, ChEBI:CHEBI:132076; Evidence={ECO:0000269|PubMed:17493578};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-taurine + O2 = N-(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-taurine; Xref=Rhea:RHEA:50156, ChEBI:CHEBI:15379, ChEBI:CHEBI:132060, ChEBI:CHEBI:132062; Evidence={ECO:0000269|PubMed:18311922};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + O2 = 2-[12-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl]-glycerol; Xref=Rhea:RHEA:63224, ChEBI:CHEBI:15379, ChEBI:CHEBI:52392, ChEBI:CHEBI:146254; Evidence={ECO:0000269|PubMed:11956198};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + O2 = 1-octadecanoyl-2-(15-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoyl)-sn-glycero-3-phosphocholine; Xref=Rhea:RHEA:63264, ChEBI:CHEBI:15379, ChEBI:CHEBI:74965, ChEBI:CHEBI:146283; Evidence={ECO:0000269|PubMed:27435673};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=a 1-acyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phospho-(1D-myo-inositol) + O2 = a 1-acyl-2-(15-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoyl)-sn-glycero-3-phospho-(1D-myo-inositol); Xref=Rhea:RHEA:63276, ChEBI:CHEBI:15379, ChEBI:CHEBI:75243, ChEBI:CHEBI:146285; Evidence={ECO:0000269|PubMed:27435673};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=a 1-acyl-2-(8Z,11Z,14Z-eicosatrienoyl)-sn-glycero-3-phospho-(1D-myo-inositol) + O2 = a 1-acyl-2-(15-hydroperoxy-8Z,11Z,13E-eicosatrienoyl)-sn-glycero-3-phospho-(1D-myo-inositol); Xref=Rhea:RHEA:63280, ChEBI:CHEBI:15379, ChEBI:CHEBI:146286, ChEBI:CHEBI:146287; Evidence={ECO:0000269|PubMed:27435673};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=1-octadecanoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphoethanolamine + O2 = 1-octadecanoyl-2-(15-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine; Xref=Rhea:RHEA:63268, ChEBI:CHEBI:15379, ChEBI:CHEBI:78268, ChEBI:CHEBI:146282; Evidence={ECO:0000269|PubMed:27435673};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phospho-(1D-myo-inositol) + O2 = 1-octadecanoyl-2-(15-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoyl)-sn-glycero-3-phospho-(1D-myo-inositol); Xref=Rhea:RHEA:63272, ChEBI:CHEBI:15379, ChEBI:CHEBI:133606, ChEBI:CHEBI:146284; Evidence={ECO:0000269|PubMed:27435673};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(8Z,11Z,14Z)-eicosatrienoate + O2 = 15-hydroperoxy-(8Z,11Z,13E)-eicosatrienoate; Xref=Rhea:RHEA:63312, ChEBI:CHEBI:15379, ChEBI:CHEBI:71589, ChEBI:CHEBI:146292; Evidence={ECO:0000269|PubMed:27435673};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(7S)-hydroperoxy-(4Z,8E,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (7S,17S)-dihydroperoxy-(4Z,8E,10Z,13Z,15E,19Z)-docosahexaenoate; Xref=Rhea:RHEA:64728, ChEBI:CHEBI:15379, ChEBI:CHEBI:140349, ChEBI:CHEBI:156049; Evidence={ECO:0000269|PubMed:32404334};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = 15-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate; Xref=Rhea:RHEA:48832, ChEBI:CHEBI:15379, ChEBI:CHEBI:32395, ChEBI:CHEBI:90821; Evidence={ECO:0000269|PubMed:10542053};.
UniProtKB/Swiss-Prot Induction:
Up-regulated by UV-irradiation.
UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=1100 uM for arachidonate (isoform D at pH 7.4 and 20 degrees Celsius) {ECO:0000269|PubMed:10542053}; KM=10 uM for linoleate (isoform A at pH 7.4 and 20 degrees Celsius) {ECO:0000269|PubMed:10542053}; KM=25 uM for (5Z,8Z,11Z,14Z)-eicosatetraenoate (isoform A at pH 7.4 and 20 degrees Celsius) {ECO:0000269|PubMed:10542053}; KM=23 uM for (5Z,8Z,11Z,14Z)-eicosatetraenoate (Sf9-expressed enzyme) {ECO:0000269|PubMed:11956198}; KM=15 uM for (5Z,8Z,11Z,14Z)-eicosatetraenoate (E. Coli-expressed enzyme) {ECO:0000269|PubMed:11956198}; KM=9 uM for 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol (Sf9-expressed enzyme) {ECO:0000269|PubMed:11956198}; KM=8 uM for 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol (E. Coli-expressed enzyme) {ECO:0000269|PubMed:11956198}; KM=14 uM for anandamide (Sf9-expressed enzyme) {ECO:0000269|PubMed:11956198}; KM=11 uM for anandamide (E. Coli-expressed enzyme) {ECO:0000269|PubMed:11956198}; KM=8 uM for (5Z,8Z,11Z,14Z)-eicosatetraenoate {ECO:0000269|PubMed:17493578}; KM=11 uM for N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine {ECO:0000269|PubMed:17493578}; KM=6 uM for N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-alanine {ECO:0000269|PubMed:17493578}; KM=8 uM for N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-gamma-aminobutanoate {ECO:0000269|PubMed:17493578}; KM=1.2 uM for (5Z,8Z,11Z,14Z)-eicosatetraenoate {ECO:0000269|PubMed:24282679}; KM=4 uM for (5Z,8Z,11Z,14Z)-eicosatetraenoate {ECO:0000269|PubMed:27145229}; KM=3.3 uM for (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate {ECO:0000269|PubMed:27145229}; KM=19 uM for (5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate {ECO:0000269|PubMed:27145229}; KM=1.74 uM for (5Z,8Z,11Z,14Z)-eicosatetraenoate {ECO:0000269|PubMed:27435673}; KM=3.46 uM for (8Z,11Z,14Z)-eicosatrienoate {ECO:0000269|PubMed:27435673}; Vmax=4 umol/min/mg enzyme with arachidonate as substrate (isoform A at pH 7.4 and 20 degrees Celsius) {ECO:0000269|PubMed:10542053}; Vmax=2 umol/min/mg enzyme with arachidonate as substrate (isoform D at pH 7.4 and 20 degrees Celsius) {ECO:0000269|PubMed:10542053}; Vmax=4 umol/min/mg enzyme with linoleate as substrate (isoform A at pH 7.4 and 20 degrees Celsius) {ECO:0000269|PubMed:10542053}; Vmax=0.82 nmol/sec/mg enzyme with (5Z,8Z,11Z,14Z)-eicosatetraenoate (Sf9-expressed enzyme) {ECO:0000269|PubMed:11956198}; Vmax=4.3 nmol/sec/mg enzyme with (5Z,8Z,11Z,14Z)-eicosatetraenoate (E. Coli-expressed enzyme) {ECO:0000269|PubMed:11956198}; Vmax=9 nmol/sec/mg enzyme with 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol (Sf9-expressed enzyme) {ECO:0000269|PubMed:11956198}; Vmax=8 nmol/sec/mg enzyme with 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol (E. Coli-expressed enzyme) {ECO:0000269|PubMed:11956198}; Vmax=14 nmol/sec/mg enzyme with anandamide (Sf9-expressed enzyme) {ECO:0000269|PubMed:11956198}; Vmax=11 nmol/sec/mg enzyme with anandamide (E. Coli-expressed enzyme) {ECO:0000269|PubMed:11956198}; Note=kcat is 2 sec(-1) for (5Z,8Z,11Z,14Z)-eicosatetraenoate. kcat is 2.1 sec(-1) for N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine. kcat is 2 sec(-1) for N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-alanine. kcat is 2 sec(-1) for N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-gamma-aminobutanoate (PubMed:17493578). kcat is 0.18 sec(-1) for (5Z,8Z,11Z,14Z)-eicosatetraenoate (PubMed:24282679). kcat is 1 sec(-1) for (5Z,8Z,11Z,14Z)-eicosatetraenoate. kcat is 2.1 sec(-1) for (5S)-hydroxy-(6E,8Z,11Z,14Z)-eicosatetraenoate. kcat is 1.5 sec(-1) for (5S)-hydroperoxy-(6E,8Z,11Z,14Z)-eicosatetraenoate (PubMed:27145229). kcat is 0.46 sec(-1) for (5Z,8Z,11Z,14Z)-eicosatetraenoate. kcat is 0.31 sec(-1) for (8Z,11Z,14Z)-eicosatrienoate (PubMed:27435673). {ECO:0000269|PubMed:17493578, ECO:0000269|PubMed:24282679, ECO:0000269|PubMed:27145229, ECO:0000269|PubMed:27435673};

Enzyme Numbers (IUBMB) for ALOX15B Gene

Phenotypes From GWAS Catalog for ALOX15B Gene

Gene Ontology (GO) - Molecular Function for ALOX15B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005506 iron ion binding IDA 24497644
GO:0005509 calcium ion binding IDA 24497644
GO:0005515 protein binding IPI 32296183
GO:0008289 lipid binding IEA --
GO:0016165 linoleate 13S-lipoxygenase activity IDA 10542053
genes like me logo Genes that share ontologies with ALOX15B: view
genes like me logo Genes that share phenotypes with ALOX15B: view

Animal Models for ALOX15B Gene

MGI Knock Outs for ALOX15B:
  • Alox8 Alox8<tm1a(KOMP)Wtsi>

miRNA for ALOX15B Gene

miRTarBase miRNAs that target ALOX15B

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ALOX15B

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for ALOX15B Gene

Localization for ALOX15B Gene

Subcellular locations from UniProtKB/Swiss-Prot for ALOX15B Gene

[Isoform A]: Nucleus. Note=Other isoforms are excluded from the nucleus. {ECO:0000269 PubMed:12704195}.
Cytoplasm, cytosol. Cell membrane. Cytoplasm, cytoskeleton. Membrane. Peripheral membrane protein. Cell junction, adherens junction. Cell junction, focal adhesion. Note=Predominantly cytosolic; becomes enriched at membranes upon calcium binding. {ECO:0000269 PubMed:10542053, ECO:0000269 PubMed:12704195, ECO:0000269 PubMed:24497644, ECO:0000269 PubMed:27435673}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ALOX15B gene
Compartment Confidence
plasma membrane 5
cytoskeleton 5
nucleus 5
cytosol 5
extracellular 4
peroxisome 2
mitochondrion 1
endoplasmic reticulum 1
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (3)
  • Plasma membrane (3)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for ALOX15B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005737 cytoplasm IEA --
GO:0005829 cytosol TAS --
GO:0005856 cytoskeleton IEA,IDA 12704195
GO:0005886 plasma membrane IEA,IDA 12704195
genes like me logo Genes that share ontologies with ALOX15B: view

Pathways & Interactions for ALOX15B Gene

genes like me logo Genes that share pathways with ALOX15B: view

Pathways by source for ALOX15B Gene

1 GeneGo (Thomson Reuters) pathway for ALOX15B Gene
  • HETE and HPETE biosynthesis and metabolism

UniProtKB/Swiss-Prot O15296-LX15B_HUMAN

  • Pathway: Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.

Gene Ontology (GO) - Biological Process for ALOX15B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006629 lipid metabolic process TAS 11839751
GO:0006644 phospholipid metabolic process IDA 27435673
GO:0006915 apoptotic process TAS 11839751
GO:0008285 negative regulation of cell proliferation IMP 12704195
GO:0010744 positive regulation of macrophage derived foam cell differentiation IMP 22912809
genes like me logo Genes that share ontologies with ALOX15B: view

No data available for SIGNOR curated interactions for ALOX15B Gene

Drugs & Compounds for ALOX15B Gene

(5) Drugs for ALOX15B Gene - From: HMDB and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
linoleic acid Approved, Experimental Pharma 0
Oxygen Approved, Vet_approved Pharma 0
arachidonic acid Experimental Pharma 41
Fe2+ Experimental Pharma 0

(7) Additional Compounds for ALOX15B Gene - From: HMDB and Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
13-L-Hydroperoxylinoleic acid
  • (9Z,11E)-(13S)-13-Hydroperoxyoctadeca-9,11-dienoate
  • (9Z,11E)-(13S)-13-Hydroperoxyoctadeca-9,11-dienoic acid
  • 13S-Hydroperoxy-9Z,11E-octadecadienoic acid
  • 13(S)-HPODE
  • 13S-Hydroperoxy-9Z,11E-octadecadienoate
33964-75-9
15(S)-HPETE
  • (5Z,8Z,11Z,13E)-(15S)-15-Hydroperoxyicosa-5,8,11,13-tetraenoate
  • (5Z,8Z,11Z,13E)-(15S)-15-Hydroperoxyicosa-5,8,11,13-tetraenoic acid
  • 15-Hydroperoxyeicosatetraenoate
  • 15-Hydroperoxyeicosatetraenoic acid
  • 15-Hydroperoxyicosatetraenoate
70981-96-3
8(S)-HPETE
  • (5Z,9E,11Z,14Z)-(8S)-8-Hydroperoxyeicosa-5,9,11,14-tetraenoic acid
  • (5Z,9E,11Z,14Z)-(8S)-8-Hydroperoxyicosa-5,9,11,14-tetraenoic acid
  • (5Z,9E,11Z,14Z)-(8S)-8-Hydroperoxyeicosa-5,9,11,14-tetraenoate
  • (5Z,9E,11Z,14Z)-(8S)-8-Hydroperoxyicosa-5,9,11,14-tetraenoate
  • 8-HPETE
genes like me logo Genes that share compounds with ALOX15B: view

Transcripts for ALOX15B Gene

mRNA/cDNA for ALOX15B Gene

3 REFSEQ mRNAs :
14 NCBI additional mRNA sequence :
5 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ALOX15B

Alternative Splicing Database (ASD) splice patterns (SP) for ALOX15B Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7 ^ 8 ^ 9 ^ 10a · 10b ^ 11 ^ 12a · 12b ^ 13 ^ 14a · 14b
SP1: - -
SP2: - - -
SP3: - - - - -
SP4:
SP5:

Relevant External Links for ALOX15B Gene

GeneLoc Exon Structure for
ALOX15B

Expression for ALOX15B Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for ALOX15B Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for ALOX15B Gene

This gene is overexpressed in Prostate (x7.5), Esophagus - Mucosa (x6.7), Lung (x6.3), Vagina (x5.5), and Skin - Not Sun Exposed (Suprapubic) (x4.7).

Protein differential expression in normal tissues from HIPED for ALOX15B Gene

This gene is overexpressed in Cervix (52.1) and Urine (8.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for ALOX15B Gene



Transcriptomic regulation report from SPP (The Signaling Pathways Project) for ALOX15B

SOURCE GeneReport for Unigene cluster for ALOX15B Gene:

Hs.111256

mRNA Expression by UniProt/SwissProt for ALOX15B Gene:

O15296-LX15B_HUMAN
Tissue specificity: Expressed in hair, prostate, lung, ovary, lymph node, spinal cord and cornea.

Evidence on tissue expression from TISSUES for ALOX15B Gene

  • Skin(4.5)
  • Lung(2.2)
genes like me logo Genes that share expression patterns with ALOX15B: view

Primer products for research

No data available for Protein tissue co-expression partners and Phenotype-based relationships between genes and organs from Gene ORGANizer for ALOX15B Gene

Orthologs for ALOX15B Gene

This gene was present in the common ancestor of chordates.

Orthologs for ALOX15B Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia ALOX15B 29 30
  • 99.06 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia ALOX15B 29 30
  • 85.4 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia ALOX15B 29 30
  • 84.32 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Alox15b 29
  • 81.26 (n)
Mouse
(Mus musculus)
Mammalia Alox8 29 16 30
  • 80.33 (n)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia -- 30
  • 59 (a)
OneToMany
-- 30
  • 53 (a)
OneToMany
Chicken
(Gallus gallus)
Aves ALOXE3 29
  • 59.95 (n)
-- 30
  • 38 (a)
OneToMany
Lizard
(Anolis carolinensis)
Reptilia -- 30
  • 53 (a)
ManyToMany
-- 30
  • 51 (a)
ManyToMany
-- 30
  • 49 (a)
ManyToMany
-- 30
  • 48 (a)
ManyToMany
-- 30
  • 48 (a)
ManyToMany
-- 30
  • 46 (a)
ManyToMany
African clawed frog
(Xenopus laevis)
Amphibia Xl.25238 29
Zebrafish
(Danio rerio)
Actinopterygii ALOXE3 30
  • 40 (a)
OneToMany
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 30
  • 34 (a)
ManyToMany
CSA.6148 30
  • 30 (a)
ManyToMany
-- 30
  • 29 (a)
ManyToMany
-- 30
  • 29 (a)
ManyToMany
Species where no ortholog for ALOX15B was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Oppossum (Monodelphis domestica)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Tropical Clawed Frog (Silurana tropicalis)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for ALOX15B Gene

ENSEMBL:
Gene Tree for ALOX15B (if available)
TreeFam:
Gene Tree for ALOX15B (if available)
Aminode:
Evolutionary constrained regions (ECRs) for ALOX15B: view image
Alliance of Genome Resources:
Additional Orthologs for ALOX15B

Paralogs for ALOX15B Gene

Paralogs for ALOX15B Gene

(6) SIMAP similar genes for ALOX15B Gene using alignment to 2 proteins:

  • LX15B_HUMAN
  • I3L1D5_HUMAN
genes like me logo Genes that share paralogs with ALOX15B: view

Variants for ALOX15B Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for ALOX15B Gene

SNP ID Clinical significance and condition Chr 17 pos Variation AA Info Type
rs111777159 Benign: not provided 8,045,477(+) C/T
NM_001141.3(ALOX15B):c.997-6C>T
INTRON
rs139800287 Likely Benign: not provided 8,042,407(+) A/G
NM_001141.3(ALOX15B):c.488A>G (p.Asp163Gly)
MISSENSE
rs140623478 Uncertain Significance: not provided 8,047,001(+) G/A
NM_001141.3(ALOX15B):c.1382G>A (p.Arg461Gln)
MISSENSE
rs141534086 Likely Benign: not provided 8,044,921(+) C/T
NM_001141.3(ALOX15B):c.769C>T (p.Leu257Phe)
MISSENSE
rs149490685 Likely Benign: not provided 8,045,375(+) C/T
NM_001141.3(ALOX15B):c.987C>T (p.Leu329=)
SYNONYMOUS

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for ALOX15B Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for ALOX15B Gene

Variant ID Type Subtype PubMed ID
nsv528325 CNV loss 19592680
nsv833352 CNV loss 17160897

Variation tolerance for ALOX15B Gene

Residual Variation Intolerance Score: 96.8% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 9.38; 88.45% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ALOX15B Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
ALOX15B
Leiden Open Variation Database (LOVD)
ALOX15B

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ALOX15B Gene

Disorders for ALOX15B Gene

MalaCards: The human disease database

(6) MalaCards diseases for ALOX15B Gene - From: COP and GCD

Disorder Aliases PubMed IDs
embryoma
  • blastoma
autosomal recessive congenital ichthyosis
  • arci
bleeding disorder, platelet-type, 8
  • bdplt8
ichthyosis
  • ichthyoses
prostate cancer
  • prostate cancer, somatic
- elite association - COSMIC cancer census association via MalaCards

Additional Disease Information for ALOX15B

Human Genome Epidemiology Navigator
(HuGE)
Atlas
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
Open Targets Platform
genes like me logo Genes that share disorders with ALOX15B: view

No data available for UniProtKB/Swiss-Prot and Genatlas for ALOX15B Gene

Publications for ALOX15B Gene

  1. Discovery of a second 15S-lipoxygenase in humans. (PMID: 9177185) Brash AR … Chang MS (Proceedings of the National Academy of Sciences of the United States of America 1997) 2 3 4 22
  2. 15-Lipoxygenase-2 expression in human macrophages induces chemokine secretion and T cell migration. (PMID: 18067895) Danielsson KN … Hultén LM (Atherosclerosis 2008) 3 4 22
  3. Subcellular localization and tumor-suppressive functions of 15-lipoxygenase 2 (15-LOX2) and its splice variants. (PMID: 12704195) Bhatia B … Tang DG (The Journal of biological chemistry 2003) 3 4 22
  4. Evidence that arachidonate 15-lipoxygenase 2 is a negative cell cycle regulator in normal prostate epithelial cells. (PMID: 11839751) Tang S … Tang DG (The Journal of biological chemistry 2002) 3 4 22
  5. A gene cluster encoding human epidermis-type lipoxygenases at chromosome 17p13.1: cloning, physical mapping, and expression. (PMID: 11350124) Krieg P … Fürstenberger G (Genomics 2001) 3 4 22

Products for ALOX15B Gene

Sources for ALOX15B Gene