ALOX12 Gene (Protein Coding)

Arachidonate 12-Lipoxygenase, 12S Type

GC17P006995
GIFtS:
44
This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on different polyunsaturated fatty acid substrates to generate bioactive lipid mediators including eicosanoids and lipoxins. The encoded enzyme and its reaction products have been shown to regulate platelet function. Elevated expression of this gene has been observed in pancreatic islets ... See more...

Aliases for ALOX12 Gene

Aliases for ALOX12 Gene

  • Arachidonate 12-Lipoxygenase, 12S Type 2 3 5
  • 12S-LOX 2 3 4
  • Polyunsaturated Fatty Acid Lipoxygenase ALOX12 3 4
  • Arachidonate 12-Lipoxygenase, 12S-Type 2 3
  • Arachidonate (12S)-Lipoxygenase 3 4
  • Arachidonate (15S)-Lipoxygenase 3 4
  • Platelet-Type Lipoxygenase 12 3 4
  • Lipoxin Synthase 12-LO 3 4
  • 12S-Lipoxygenase 3 4
  • Platelet 12-LOX 2 3
  • LOG12 3 4
  • Arachidonate 15-Lipoxygenase,15S-Type 3
  • Platelet-Type 12-Lipoxygenase 3
  • Arachidonate 12-Lipoxygenase 2
  • Linoleate (13S)-Lipoxygenase 4
  • Linoleate 13S-Lipoxygenase 3
  • EC 1.13.11.31 4
  • EC 1.13.11.33 4
  • EC 1.13.11.- 4
  • EC 1.13.11 50
  • EC 3.3.2.- 4
  • 12-LOX 3
  • ALOX12 5
  • 12LO 4

External Ids for ALOX12 Gene

Previous GeneCards Identifiers for ALOX12 Gene

  • GC17P007287
  • GC17P007630
  • GC17P006741
  • GC17P007099
  • GC17P006840
  • GC17P006899
  • GC17P006998

Summaries for ALOX12 Gene

Entrez Gene Summary for ALOX12 Gene

  • This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on different polyunsaturated fatty acid substrates to generate bioactive lipid mediators including eicosanoids and lipoxins. The encoded enzyme and its reaction products have been shown to regulate platelet function. Elevated expression of this gene has been observed in pancreatic islets derived from human diabetes patients. Allelic variants in this gene may be associated with susceptibility to toxoplasmosis. Multiple pseudogenes of this gene have been identified in the human genome. [provided by RefSeq, Aug 2017]

GeneCards Summary for ALOX12 Gene

ALOX12 (Arachidonate 12-Lipoxygenase, 12S Type) is a Protein Coding gene. Diseases associated with ALOX12 include Toxoplasmosis and Squamous Cell Carcinoma. Among its related pathways are HETE and HPETE biosynthesis and metabolism and Antifolate resistance. Gene Ontology (GO) annotations related to this gene include iron ion binding and arachidonate 12-lipoxygenase activity. An important paralog of this gene is ALOX15.

UniProtKB/Swiss-Prot Summary for ALOX12 Gene

  • Catalyzes the regio and stereo-specific incorporation of molecular oxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed:17493578, PubMed:1851637, PubMed:8319693, PubMed:8500694, PubMed:18311922, PubMed:32404334). Mainly converts arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to the specific bioactive lipid (12S)-hydroperoxyeicosatetraenoate/(12S)-HPETE (PubMed:17493578, PubMed:22984144, PubMed:24282679, PubMed:8319693, PubMed:8500694). Through the production of bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation (PubMed:8319693, PubMed:8500694). It can also catalyze the epoxidation of double bonds of polyunsaturated fatty acids such as (14S)-hydroperoxy-docosahexaenoate/(14S)-HPDHA resulting in the formation of (13S,14S)-epoxy-DHA (PubMed:23504711). Furthermore, it may participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, that actively downregulate the immune response and have anti-aggregation properties with platelets (PubMed:32404334). An additional function involves a multistep process by which it transforms leukotriene A4/LTA4 into the bioactive lipids lipoxin A4/LXA4 and lipoxin B4/LXB4, both are vasoactive and LXA4 may regulate neutrophil function via occupancy of specific recognition sites (PubMed:8250832). Can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to (13S)-hydroperoxyoctadecadienoate/ (13S-HPODE) (By similarity). Due to its role in regulating both the expression of the vascular endothelial growth factor (VEGF, an angiogenic factor involved in the survival and metastasis of solid tumors) and the expression of integrin beta-1 (known to affect tumor cell migration and proliferation), it can be regarded as protumorigenic (PubMed:9751607, PubMed:16638750, PubMed:22237009). Important for cell survival, as it may play a role not only in proliferation but also in the prevention of apoptosis in vascular smooth muscle cells (PubMed:23578768).

Tocris Summary for ALOX12 Gene

  • Lipoxygenases (LOXs) are a family of non-heme iron dioxygenases that are involved in the production and metabolism of fatty acid hydroperoxidases. There are six lipoxygenase isozymes; 5-LOX, 12R-LOX, 12S-LOX, 15-LOX-1, 15-LOX-2 and E-LOX.

Gene Wiki entry for ALOX12 Gene

Additional gene information for ALOX12 Gene

No data available for CIViC Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for ALOX12 Gene

Genomics for ALOX12 Gene

GeneHancer (GH) Regulatory Elements Pubs

Download GeneHancer 2017 data
Promoters and enhancers for ALOX12 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around ALOX12 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for ALOX12

Top Transcription factor binding sites by QIAGEN in the ALOX12 gene promoter:
  • AML1a
  • FOXL1
  • Lmo2
  • LyF-1
  • Nkx2-5
  • YY1
  • Zic1
  • ZIC2

Genomic Locations for ALOX12 Gene

Latest Assembly
chr17:6,993,791-7,010,754
(GRCh38/hg38)
Size:
16,964 bases
Orientation:
Plus strand

Previous Assembly
chr17:6,899,368-6,914,073
(GRCh37/hg19 by Entrez Gene)
Size:
14,706 bases
Orientation:
Plus strand

chr17:6,899,384-6,914,055
(GRCh37/hg19 by Ensembl)
Size:
14,672 bases
Orientation:
Plus strand

Genomic View for ALOX12 Gene

Genes around ALOX12 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ALOX12 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ALOX12 Gene
GeneLoc Logo Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for ALOX12 Gene

Proteins for ALOX12 Gene

Products:

  1. Antibodies for research
  2. Protein products for research
  3. Assay products for research

  • Protein details for ALOX12 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P18054-LOX12_HUMAN
    Recommended name:
    Polyunsaturated fatty acid lipoxygenase ALOX12
    Protein Accession:
    P18054
    Secondary Accessions:
    • O95569
    • Q6ISF8
    • Q9UQM4

    Protein attributes for ALOX12 Gene

    Size:
    663 amino acids
    Molecular mass:
    75694 Da
    Cofactor:
    Name=Fe cation; Xref=ChEBI:CHEBI:24875;
    Quaternary structure:
    No Data Available

    Three dimensional structures from OCA and Proteopedia for ALOX12 Gene

neXtProt entry for ALOX12 Gene

Protein Expression for ALOX12 Gene

See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB

Selected DME Specific Peptides for ALOX12 Gene

P18054:
  • CPPDDLA
  • GMEELQAQLEKEL
  • WVPNAPCTMR

Post-translational modifications for ALOX12 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for ALOX12 Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for ALOX12 Gene

Gene Families for ALOX12 Gene

HGNC:
IUPHAR :
Human Protein Atlas (HPA):
  • Disease related genes
  • Enzymes
  • Potential drug targets
  • Predicted intracellular proteins

Protein Domains for ALOX12 Gene

InterPro:
Blocks:
  • Lipoxygenase, LH2 domain
  • Lipoxygenase
  • Mammalian lipoxygenase signature

Suggested Antigen Peptide Sequences for ALOX12 Gene

GenScript: Design optimal peptide antigens:
  • Platelet-type lipoxygenase 12 (LOX12_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P18054

UniProtKB/Swiss-Prot:

LOX12_HUMAN :
  • Belongs to the lipoxygenase family.
Family:
  • Belongs to the lipoxygenase family.
genes like me logo Genes that share domains with ALOX12: view

Function for ALOX12 Gene

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  2. CRISPR products for research
  3. miRNA products for research
  4. Inhibitory RNAs for research
  5. Clone products for research
  6. Cell Lines for research

Molecular function for ALOX12 Gene

UniProtKB/Swiss-Prot Function:
Catalyzes the regio and stereo-specific incorporation of molecular oxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed:17493578, PubMed:1851637, PubMed:8319693, PubMed:8500694, PubMed:18311922, PubMed:32404334). Mainly converts arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to the specific bioactive lipid (12S)-hydroperoxyeicosatetraenoate/(12S)-HPETE (PubMed:17493578, PubMed:22984144, PubMed:24282679, PubMed:8319693, PubMed:8500694). Through the production of bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation (PubMed:8319693, PubMed:8500694). It can also catalyze the epoxidation of double bonds of polyunsaturated fatty acids such as (14S)-hydroperoxy-docosahexaenoate/(14S)-HPDHA resulting in the formation of (13S,14S)-epoxy-DHA (PubMed:23504711). Furthermore, it may participate in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) like resolvin D5 ((7S,17S)-diHPDHA) and (7S,14S)-diHPDHA, that actively downregulate the immune response and have anti-aggregation properties with platelets (PubMed:32404334). An additional function involves a multistep process by which it transforms leukotriene A4/LTA4 into the bioactive lipids lipoxin A4/LXA4 and lipoxin B4/LXB4, both are vasoactive and LXA4 may regulate neutrophil function via occupancy of specific recognition sites (PubMed:8250832). Can also peroxidize linoleate ((9Z,12Z)-octadecadienoate) to (13S)-hydroperoxyoctadecadienoate/ (13S-HPODE) (By similarity). Due to its role in regulating both the expression of the vascular endothelial growth factor (VEGF, an angiogenic factor involved in the survival and metastasis of solid tumors) and the expression of integrin beta-1 (known to affect tumor cell migration and proliferation), it can be regarded as protumorigenic (PubMed:9751607, PubMed:16638750, PubMed:22237009). Important for cell survival, as it may play a role not only in proliferation but also in the prevention of apoptosis in vascular smooth muscle cells (PubMed:23578768).
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate; Xref=Rhea:RHEA:10428, ChEBI:CHEBI:15379, ChEBI:CHEBI:32395, ChEBI:CHEBI:57444; EC=1.13.11.31; Evidence={ECO:0000269|PubMed:17493578, ECO:0000269|PubMed:22984144, ECO:0000269|PubMed:24282679, ECO:0000269|PubMed:8319693, ECO:0000269|PubMed:8500694};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoate + O2 = (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate; Xref=Rhea:RHEA:16869, ChEBI:CHEBI:15379, ChEBI:CHEBI:32395, ChEBI:CHEBI:57446; EC=1.13.11.33; Evidence={ECO:0000269|PubMed:17493578};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=2 H2O + 2 leukotriene A4 + O2 = 2 lipoxin A4; Xref=Rhea:RHEA:48584, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57463, ChEBI:CHEBI:67026; Evidence={ECO:0000269|PubMed:1851637, ECO:0000269|PubMed:8250832};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=2 H2O + 2 leukotriene A4 + O2 = 2 lipoxin B4; Xref=Rhea:RHEA:48588, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:57463, ChEBI:CHEBI:67031; Evidence={ECO:0000269|PubMed:8250832};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(14S)-hydroperoxy-(4Z,7Z,10Z,12E,16Z,19Z)-docosahexaenoate = (13S,14S)-epoxy-(4Z,7Z,9E,11E,16Z,19Z)-docosahexaenoate + H2O; Xref=Rhea:RHEA:53532, ChEBI:CHEBI:15377, ChEBI:CHEBI:78048, ChEBI:CHEBI:131958; Evidence={ECO:0000269|PubMed:23504711};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-alanine + O2 = N-(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-alanine; Xref=Rhea:RHEA:50184, ChEBI:CHEBI:15379, ChEBI:CHEBI:132071, ChEBI:CHEBI:132077; Evidence={ECO:0000269|PubMed:17493578};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-alanine + O2 = N-(12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-alanine; Xref=Rhea:RHEA:50172, ChEBI:CHEBI:15379, ChEBI:CHEBI:132071, ChEBI:CHEBI:132074; Evidence={ECO:0000269|PubMed:17493578};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-gamma-aminobutanoate + O2 = N-(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-gamma-aminobutanoate; Xref=Rhea:RHEA:50180, ChEBI:CHEBI:15379, ChEBI:CHEBI:132072, ChEBI:CHEBI:132078; Evidence={ECO:0000269|PubMed:17493578};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-gamma-aminobutanoate + O2 = N-(12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-gamma-aminobutanoate; Xref=Rhea:RHEA:50176, ChEBI:CHEBI:15379, ChEBI:CHEBI:132072, ChEBI:CHEBI:132075; Evidence={ECO:0000269|PubMed:17493578};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine + O2 = N-(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-glycine; Xref=Rhea:RHEA:50188, ChEBI:CHEBI:15379, ChEBI:CHEBI:59002, ChEBI:CHEBI:132076; Evidence={ECO:0000269|PubMed:17493578};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine + O2 = N-(12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-glycine; Xref=Rhea:RHEA:50168, ChEBI:CHEBI:15379, ChEBI:CHEBI:59002, ChEBI:CHEBI:132073; Evidence={ECO:0000269|PubMed:17493578};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-taurine + O2 = N-(12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoyl-taurine; Xref=Rhea:RHEA:50160, ChEBI:CHEBI:15379, ChEBI:CHEBI:132060, ChEBI:CHEBI:132061; Evidence={ECO:0000269|PubMed:18311922};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-taurine + O2 = N-(15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl-taurine; Xref=Rhea:RHEA:50156, ChEBI:CHEBI:15379, ChEBI:CHEBI:132060, ChEBI:CHEBI:132062; Evidence={ECO:0000269|PubMed:18311922};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (14S)-hydroperoxy-(4Z,7Z,10Z,12E,16Z,19Z)-docosahexaenoate; Xref=Rhea:RHEA:41332, ChEBI:CHEBI:15379, ChEBI:CHEBI:77016, ChEBI:CHEBI:78048; Evidence={ECO:0000269|PubMed:25036362, ECO:0000269|PubMed:32404334};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(7S)-hydroperoxy-(4Z,8E,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (7S,14S)-dihydroperoxy-(4Z,8E,10Z,12E,16Z,19Z)-docosahexaenoate; Xref=Rhea:RHEA:64724, ChEBI:CHEBI:15379, ChEBI:CHEBI:156049, ChEBI:CHEBI:156082; Evidence={ECO:0000269|PubMed:32404334};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(7S)-hydroperoxy-(4Z,8E,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 = (7S,17S)-dihydroperoxy-(4Z,8E,10Z,13Z,15E,19Z)-docosahexaenoate; Xref=Rhea:RHEA:64728, ChEBI:CHEBI:15379, ChEBI:CHEBI:140349, ChEBI:CHEBI:156049; Evidence={ECO:0000269|PubMed:32404334};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + O2 = 12S-HpEPE; Xref=Rhea:RHEA:48704, ChEBI:CHEBI:15379, ChEBI:CHEBI:58562, ChEBI:CHEBI:90772; Evidence={ECO:0000269|PubMed:22984144};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(8Z,11Z,14Z)-eicosatrienoate + O2 = (12S)-hydroperoxy-(8Z,10E,14Z)-eicosatrienoate; Xref=Rhea:RHEA:41328, ChEBI:CHEBI:15379, ChEBI:CHEBI:71589, ChEBI:CHEBI:78047; Evidence={ECO:0000269|PubMed:22984144};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(9Z,12Z)-octadecadienoate + O2 = (13S)-hydroperoxy-(9Z,11E)-octadecadienoate; Xref=Rhea:RHEA:22780, ChEBI:CHEBI:15379, ChEBI:CHEBI:30245, ChEBI:CHEBI:57466;.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(5Z,8Z,11Z)-eicosatrienoate + O2 = (12S)-hydroperoxy-(5Z,8Z,10E)-eicosatrienoate; Xref=Rhea:RHEA:41324, ChEBI:CHEBI:15379, ChEBI:CHEBI:78043, ChEBI:CHEBI:78046; Evidence={ECO:0000250|UniProtKB:P39655};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(14R,15S)-epoxy-(5Z,8Z,11Z)-eicosatrienoate + O2 = (12S)-hydroperoxy-(14R,15S)-epoxy-(5Z,8Z,10E)-eicosatrienoate; Xref=Rhea:RHEA:50276, ChEBI:CHEBI:15379, ChEBI:CHEBI:131965, ChEBI:CHEBI:132063; Evidence={ECO:0000250|UniProtKB:P39655};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(14S,15R)-epoxy-(5Z,8Z,11Z)-eicosatrienoate + O2 = (12S)-hydroperoxy-(14S,15R)-epoxy-(5Z,8Z,10E)-eicosatrienoate; Xref=Rhea:RHEA:50284, ChEBI:CHEBI:15379, ChEBI:CHEBI:131964, ChEBI:CHEBI:132065; Evidence={ECO:0000250|UniProtKB:P39655};.
UniProtKB/Swiss-Prot Induction:
Down-regulated upon starvation, by UV-irradiation and 15-lipoxygenase metabolites.
UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=8 uM for arachidonate (at pH 7.0 and 37 degrees Celsius) {ECO:0000269|PubMed:1851637}; KM=10 uM for arachidonate (at pH 8.0 and 25 degrees Celsius) {ECO:0000269|PubMed:8319693}; KM=6.2 uM for arachidonate (at pH 7.4) {ECO:0000269|PubMed:8250832}; KM=9 uM for linoleate (at pH 8.0 and 25 degrees Celsius) {ECO:0000269|PubMed:8319693}; KM=7.9 uM for leukotriene A4 (at pH 7.4) {ECO:0000269|PubMed:8250832}; KM=3 uM for eicosa-5,8,11,14,17-pentaenoate (at pH 7.0 and 37 degrees Celsius) {ECO:0000269|PubMed:1851637}; KM=35 uM for eicosa-8,11,14-trienoate (at pH 7.0 and 37 degrees Celsius) {ECO:0000269|PubMed:1851637}; KM=14.3 uM for 5,6-epoxy-8,11,14-eicosatrienoate (at pH 7.4) {ECO:0000269|PubMed:8250832}; KM=9.5 uM for arachidonic acid {ECO:0000269|PubMed:17493578}; KM=10.1 uM for N-arachidonoylglycine {ECO:0000269|PubMed:17493578}; KM=15.7 uM for N-arachidonoyl-L-alanine {ECO:0000269|PubMed:17493578}; KM=10.9 uM for N-arachidonoyl-gamma-aminobutyric acid {ECO:0000269|PubMed:17493578}; KM=9.7 uM for arachidonic acid {ECO:0000269|PubMed:25036362}; KM=5.1 uM for docosahexaenoate {ECO:0000269|PubMed:25036362}; KM=12.6 uM for arachidonic acid (in presence of calcium dichloride) {ECO:0000269|PubMed:25036362}; KM=7.1 uM for docosahexaenoate (in presence of calcium dichloride) {ECO:0000269|PubMed:25036362}; Vmax=3 umol/min/mg enzyme with arachidonate as substrate (at pH 8.0 and 25 degrees Celsius) {ECO:0000269|PubMed:8319693}; Vmax=1.057 umol/min/mg enzyme with arachidonate as substrate (at pH 7.4) {ECO:0000269|PubMed:8250832}; Vmax=0.0375 umol/min/mg enzyme with linoleate as substrate (at pH 8.0 and 25 degrees Celsius) {ECO:0000269|PubMed:8319693}; Vmax=0.025 umol/min/mg enzyme with leukotriene A4 as substrate (at pH 7.4) {ECO:0000269|PubMed:8250832}; Vmax=0.985 umol/min/mg enzyme with 5,6-epoxy-8,11,14-eicosatrienoate as substrate (at pH 7.4) {ECO:0000269|PubMed:8250832}; pH dependence: Optimum pH is 7.5-8.0 (for arachidonate 12-lipoxygenase activity). {ECO:0000269|PubMed:8250832, ECO:0000269|PubMed:8319693};
UniProtKB/Swiss-Prot EnzymeRegulation:
Activated by EGF (PubMed:8912711). Arachidonic acid conversion is inhibited by (13S,14S)-epoxy-(4Z,7Z,9E,11E,16Z,19Z)-docosahexaenoate (13S,14S-epoxy-DHA) (PubMed:23504711). Arachidonate 12-lipoxygenase activity is decreased when PH decreases from 7.4 to 6 (By similarity).
GENATLAS Biochemistry:
arachidonate-12-lipoxygenase,75kDa,lipid peroxidating enzyme implicated in the pathogenesis of inflammatory disorders as well as membrane remodeling,carcinogenesis,and atheroma formation,expressed in platelets erythroleukemia cells and umbilical vein endothelial cells,also in B cells and adult skin

Enzyme Numbers (IUBMB) for ALOX12 Gene

Phenotypes From GWAS Catalog for ALOX12 Gene

Gene Ontology (GO) - Molecular Function for ALOX12 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004052 arachidonate 12-lipoxygenase activity IBA,IDA 1851637
GO:0005506 iron ion binding IEA --
GO:0005515 protein binding IPI 10727209
GO:0016165 linoleate 13S-lipoxygenase activity IBA,IDA 8319693
GO:0016491 oxidoreductase activity IEA --
genes like me logo Genes that share ontologies with ALOX12: view
genes like me logo Genes that share phenotypes with ALOX12: view

Animal Models for ALOX12 Gene

MGI Knock Outs for ALOX12:

Animal Models for research

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ALOX12

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for ALOX12 Gene

Localization for ALOX12 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ALOX12 Gene

Cytoplasm, cytosol. Membrane. Note=Membrane association is stimulated by EGF.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ALOX12 gene
Compartment Confidence
plasma membrane 5
cytosol 5
extracellular 4
mitochondrion 3
nucleus 3
cytoskeleton 2
peroxisome 2
endoplasmic reticulum 1
endosome 1
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (3)
  • Vesicles (2)
  • Nuclear speckles (1)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for ALOX12 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IEA,IDA 8319693
GO:0005829 cytosol TAS --
GO:0005886 plasma membrane IBA 21873635
GO:0016020 membrane IEA,IDA 8912711
GO:0042383 sarcolemma IEA,IDA 15107407
genes like me logo Genes that share ontologies with ALOX12: view

Pathways & Interactions for ALOX12 Gene

genes like me logo Genes that share pathways with ALOX12: view

Pathways by source for ALOX12 Gene

2 BioSystems pathways for ALOX12 Gene
1 GeneGo (Thomson Reuters) pathway for ALOX12 Gene
  • HETE and HPETE biosynthesis and metabolism

UniProtKB/Swiss-Prot P18054-LOX12_HUMAN

  • Pathway: Lipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.

Gene Ontology (GO) - Biological Process for ALOX12 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006629 lipid metabolic process IEA,IDA 17493578
GO:0006631 fatty acid metabolic process IEA --
GO:0007568 aging IEA --
GO:0010628 positive regulation of gene expression IEA --
GO:0010656 negative regulation of muscle cell apoptotic process IMP 23578768
genes like me logo Genes that share ontologies with ALOX12: view

No data available for SIGNOR curated interactions for ALOX12 Gene

Drugs & Compounds for ALOX12 Gene

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(38) Drugs for ALOX12 Gene - From: PharmGKB, ApexBio, HMDB, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Oxygen Approved, Vet_approved Pharma 0
Celecoxib Approved, Investigational Pharma Selective cyclooxygenase-2 (COX-2) inhibitor 561
arachidonic acid Experimental Pharma 41
Fe2+ Experimental Pharma 0
Baicalein Investigational Pharma Lipoxygenases inhibitor, 5- and 12-Lipoxygenase inhibitor 1

(48) Additional Compounds for ALOX12 Gene - From: HMDB and Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
12(S)-HPETE
  • (5Z,8Z,10E,14Z)-(12S)-12-Hydroperoxyeicosa-5,8,10,14-tetraenoate
  • (5Z,8Z,10E,14Z)-(12S)-12-Hydroperoxyicosa-5,8,10,14-tetraenoate
  • (5Z,8Z,10E,14Z)-(12S)-12-Hydroperoxyicosa-5,8,10,14-tetraenoic acid
  • 12-HpETE
  • 12-Hydroperoxyeicosatetraenoate
 71774-10-2

(5) Tocris Compounds for ALOX12 Gene

Compound Action Cas Number
2-TEDC 5-, 12-, 15-Lipoxygenase inhibitor 132465-10-2
Baicalein 5- and 12-Lipoxygenase inhibitor 491-67-8
BAY-X 1005 Orally active 5-lipoxygenase activating protein (FLAP) inhibitor 128253-31-6
BW-B 70C 5-Lipoxygenase inhibitor 134470-38-5
MK 886 Inhibitor of 5-lipoxygenase-activating protein (FLAP) 118414-82-7

(1) ApexBio Compounds for ALOX12 Gene

Compound Action Cas Number
Baicalein Lipoxygenases inhibitor 491-67-8
genes like me logo Genes that share compounds with ALOX12: view

Drug products for research

Transcripts for ALOX12 Gene

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  3. Inhibitory RNAs for research
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mRNA/cDNA for ALOX12 Gene

1 REFSEQ mRNAs :
7 NCBI additional mRNA sequence :
3 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ALOX12

Alternative Splicing Database (ASD) splice patterns (SP) for ALOX12 Gene

No ASD Table

Relevant External Links for ALOX12 Gene

GeneLoc Exon Structure for
ALOX12

Expression for ALOX12 Gene

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  1. Primer products for research
  2. Gene Expression Assays for research

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for ALOX12 Gene

mRNA expression in normal human tissues for ALOX12 Gene
mRNA expression by GTEx for ALOX12 GenemRNA expression by BioGPS for ALOX12 Gene

mRNA differential expression in normal tissues according to GTEx for ALOX12 Gene

This gene is overexpressed in Vagina (x20.5), Esophagus - Mucosa (x9.5), and Cervix - Ectocervix (x7.1).

Protein differential expression in normal tissues from HIPED for ALOX12 Gene

This gene is overexpressed in Cervix (24.9), Peripheral blood mononuclear cells (19.1), and Platelet (17.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for ALOX12 Gene



Transcriptomic regulation report from SPP (The Signaling Pathways Project) for ALOX12

SOURCE GeneReport for Unigene cluster for ALOX12 Gene:

Hs.654431

mRNA Expression by UniProt/SwissProt for ALOX12 Gene:

P18054-LOX12_HUMAN
Tissue specificity: Expressed in vascular smooth muscle cells.

Evidence on tissue expression from TISSUES for ALOX12 Gene

  • Skin(4.5)
  • Blood(3.3)
  • Nervous system(2.4)
  • Heart(2.3)
  • Muscle(2.3)
  • Intestine(2.3)
genes like me logo Genes that share expression patterns with ALOX12: view

Primer products for research

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , Protein tissue co-expression partners and Phenotype-based relationships between genes and organs from Gene ORGANizer for ALOX12 Gene

Orthologs for ALOX12 Gene

This gene was present in the common ancestor of chordates.

Orthologs for ALOX12 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
 Mammalia ALOX12 29 30
  • 99.24 (n)
 OneToOne
Cow
(Bos Taurus)
 Mammalia ALOX12 29 30
  • 88.89 (n)
 OneToOne
Dog
(Canis familiaris)
 Mammalia ALOX12 29 30
  • 87.53 (n)
 OneToOne
Platypus
(Ornithorhynchus anatinus)
 Mammalia -- 30
  • 87 (a)
 OneToMany
Mouse
(Mus musculus)
 Mammalia Alox12 29 16 30
  • 85.42 (n)
 OneToOne
Rat
(Rattus norvegicus)
 Mammalia Alox12 29
  • 84.97 (n)
Oppossum
(Monodelphis domestica)
 Mammalia -- 30
  • 67 (a)
 OneToMany
-- 30
  • 64 (a)
 OneToMany
-- 30
  • 58 (a)
 OneToMany
-- 30
  • 32 (a)
 OneToMany
Zebrafish
(Danio rerio)
 Actinopterygii alox12 29 30
  • 53.39 (n)
 OneToMany
wufb72a11 29
Sea Squirt
(Ciona savignyi)
 Ascidiacea -- 30
  • 32 (a)
 ManyToMany
-- 30
  • 29 (a)
 ManyToMany
CSA.6148 30
  • 29 (a)
 ManyToMany
-- 30
  • 28 (a)
 ManyToMany
Species where no ortholog for ALOX12 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Lizard (Anolis carolinensis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Tropical Clawed Frog (Silurana tropicalis)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for ALOX12 Gene

ENSEMBL:
Gene Tree for ALOX12 (if available)
TreeFam:
Gene Tree for ALOX12 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for ALOX12: view image
Alliance of Genome Resources:
Additional Orthologs for ALOX12

Paralogs for ALOX12 Gene

Paralogs for ALOX12 Gene

(6) SIMAP similar genes for ALOX12 Gene using alignment to 2 proteins:

  • LOX12_HUMAN
  • K7ENN9_HUMAN

Pseudogenes.org Pseudogenes for ALOX12 Gene

genes like me logo Genes that share paralogs with ALOX12: view

Variants for ALOX12 Gene

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Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for ALOX12 Gene

SNP ID Clinical significance and condition Chr 17 pos Variation AA Info Type
rs115276151 Benign: not provided 6,998,976(+) G/A
NM_000697.3(ALOX12):c.566G>A (p.Arg189His) 		MISSENSE
rs11571342 Benign: not provided 7,005,898(+) G/Ap.Arg430His
NM_000697.3(ALOX12):c.1289G>A (p.Arg430His) 		MISSENSE
rs147158964 Likely Benign: not provided 7,005,306(+) G/A
NM_000697.3(ALOX12):c.1211G>A (p.Arg404Gln) 		MISSENSE
rs1126667 -- p.Gln261Arg
rs114985038 -- p.Asp134His

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for ALOX12 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for ALOX12 Gene

Variant ID Type Subtype PubMed ID
esv1008363 CNV loss 20482838
esv2422288 CNV deletion 17116639
nsv457659 CNV loss 19166990
nsv574322 CNV loss 21841781

Variation tolerance for ALOX12 Gene

Residual Variation Intolerance Score: 75.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 8.21; 84.91% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ALOX12 Gene

Human Gene Mutation Database (HGMD)
ALOX12
SNPedia medical, phenotypic, and genealogical associations of SNPs for
ALOX12
Leiden Open Variation Database (LOVD)
ALOX12

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ALOX12 Gene

Disorders for ALOX12 Gene

MalaCards: The human disease database

(13) MalaCards diseases for ALOX12 Gene - From: COP and GCD

Disorder Aliases PubMed IDs
toxoplasmosis
  • disseminated toxoplasmosis
squamous cell carcinoma
  • epidermoid carcinoma
adenoma
  • acinar cell adenoma
acute pericementitis
  • acute periodontitis
prostate cancer
  • prostate cancer, somatic
- elite association - COSMIC cancer census association via MalaCards

UniProtKB/Swiss-Prot

LOX12_HUMAN
  • Esophageal cancer (ESCR) [MIM:133239]: A malignancy of the esophagus. The most common types are esophageal squamous cell carcinoma and adenocarcinoma. Cancer of the esophagus remains a devastating disease because it is usually not detected until it has progressed to an advanced incurable stage. {ECO:0000269 PubMed:17460548}. Note=Disease susceptibility may be associated with variants affecting the gene represented in this entry. Gln at position 261 may confer interindividual susceptibility to esophageal cancer (PubMed:17460548). {ECO:0000269 PubMed:17460548}.
  • Colorectal cancer (CRC) [MIM:114500]: A complex disease characterized by malignant lesions arising from the inner wall of the large intestine (the colon) and the rectum. Genetic alterations are often associated with progression from premalignant lesion (adenoma) to invasive adenocarcinoma. Risk factors for cancer of the colon and rectum include colon polyps, long-standing ulcerative colitis, and genetic family history. {ECO:0000269 PubMed:17151091}. Note=Disease susceptibility may be associated with variants affecting the gene represented in this entry. Gln at position 261 may confer interindividual susceptibility to colorectal cancer (PubMed:17460548). {ECO:0000269 PubMed:17460548}.

Additional Disease Information for ALOX12

Human Genome Epidemiology Navigator
(HuGE)
Atlas
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
Open Targets Platform
genes like me logo Genes that share disorders with ALOX12: view

No data available for Genatlas for ALOX12 Gene

Publications for ALOX12 Gene

  1. Platelet 12-lipoxygenase Arg261Gln polymorphism: functional characterization and association with risk of esophageal squamous cell carcinoma in combination with COX-2 polymorphisms. (PMID: 17460548) Guo Y … Lin D (Pharmacogenetics and genomics 2007) 3 4 22 40
  2. Associations of functional polymorphisms in cyclooxygenase-2 and platelet 12-lipoxygenase with risk of occurrence and advanced disease status of colorectal cancer. (PMID: 17151091) Tan W … Lin D (Carcinogenesis 2007) 3 4 22 40
  3. Variation in eicosanoid genes, non-fatal myocardial infarction and ischemic stroke. (PMID: 19046748) Lemaitre RN … Psaty BM (Atherosclerosis 2009) 3 22 40
  4. Reciprocal regulation of 12- and 15-lipoxygenases by UV-irradiation in human keratinocytes. (PMID: 18755188) Yoo H … Kim TY (FEBS letters 2008) 3 4 22
  5. Association of arachidonate 12-lipoxygenase genotype variation and glycemic control with albuminuria in type 2 diabetes. (PMID: 18640486) Liu Y … Rich SS (American journal of kidney diseases : the official journal of the National Kidney Foundation 2008) 3 22 40

ExternalLinks

View latest publications for ALOX12 gene in Mastermind

Products for ALOX12 Gene

Sources for ALOX12 Gene