This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, a catalytically active thioredoxin domain, and a C-terminal ER-retention sequence. This gene is expressed in ciliated airway epithelial cells a... See more...

Aliases for AGR3 Gene

Aliases for AGR3 Gene

  • Anterior Gradient 3, Protein Disulphide Isomerase Family Member 2 3 5
  • Protein Disulfide Isomerase Family A, Member 18 2 3 4
  • Breast Cancer Membrane Protein 11 2 3 4
  • BCMP11 2 3 4
  • PDIA18 2 3 4
  • HAG-3 2 3 4
  • Anterior Gradient Protein 3 3 4
  • AG-3 3 4
  • HAG3 2 3
  • AG3 3 4
  • Anterior Gradient 3 Homolog (Xenopus Laevis) 2
  • Anterior Gradient Protein 3 Homolog 3
  • Anterior Gradient Homolog 3 3
  • Anterior Gradient 3 Homolog 4
  • AGR3 5

External Ids for AGR3 Gene

Previous GeneCards Identifiers for AGR3 Gene

  • GC07M016866
  • GC07M016899
  • GC07M016859

Summaries for AGR3 Gene

Entrez Gene Summary for AGR3 Gene

  • This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, a catalytically active thioredoxin domain, and a C-terminal ER-retention sequence. This gene is expressed in ciliated airway epithelial cells and, in mouse, plays a role in ciliary beat frequency in multiciliated cells. This gene is also over-expressed in breast, ovarian, and prostrate cancers. [provided by RefSeq, Dec 2016]

GeneCards Summary for AGR3 Gene

AGR3 (Anterior Gradient 3, Protein Disulphide Isomerase Family Member) is a Protein Coding gene. Diseases associated with AGR3 include Breast Abscess and Ritter's Disease. Gene Ontology (GO) annotations related to this gene include dystroglycan binding. An important paralog of this gene is AGR2.

UniProtKB/Swiss-Prot Summary for AGR3 Gene

  • Required for calcium-mediated regulation of ciliary beat frequency and mucociliary clearance in the airway. Might be involved in the regulation of intracellular calcium in tracheal epithelial cells.

Gene Wiki entry for AGR3 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for AGR3 Gene

Genomics for AGR3 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for AGR3 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH07J016878 Promoter/Enhancer 1 EPDnew ENCODE 608 +1.3 1328 4.4 ZNF512 FOXA1 RFX1 ZFX ZNF512B BMI1 CTBP1 ZNF217 NR2F2 MBD2 AGR3 lnc-AGR2-1 AGR2 TSPAN13 RAD17P1
GH07J016801 Promoter/Enhancer 1.4 EPDnew Ensembl ENCODE 17.5 +78.0 77950 5.9 CTCF ZHX2 FOXA1 REST RAD21 GTF2F1 ZFX SP1 CEBPB ZNF444 AGR2 lnc-AGR3-2 AGR3 TSPAN13 ENSG00000270593 piR-50308-576
GH07J016905 Enhancer 0.9 Ensembl ENCODE 22.9 -23.9 -23895 1.4 TEAD4 FOXA1 ZIC2 REST SOX13 SP1 ATF3 NR3C1 ZNF217 TEAD1 AGR3 AGR2 AHR LOC100131425 lnc-AHR-1
GH07J016913 Enhancer 1 Ensembl ENCODE 16.7 -33.8 -33817 5.1 GATAD2A CTCF PRDM10 TFE3 NFKBIZ RCOR2 JUND FOXA1 RXRB REST AHR AGR3 AGR2 TSPAN13 lnc-AHR-1 LOC100131425
GH07J016810 Enhancer 0.8 Ensembl ENCODE 17.6 +70.0 69986 4 RNF2 CREB1 RFX1 FOXA1 PRDM1 ATF7 CEBPB SP1 BMI1 NR3C1 AGR2 AGR3 ENSG00000270593 TSPAN13 lnc-AGR3-2
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around AGR3 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for AGR3

Top Transcription factor binding sites by QIAGEN in the AGR3 gene promoter:
  • AML1a
  • AREB6
  • Brachyury
  • FOXJ2
  • FOXJ2 (long isoform)
  • GATA-1
  • GATA-2
  • GATA-3
  • Hand1
  • TBP

Genomic Locations for AGR3 Gene

Genomic Locations for AGR3 Gene
chr7:16,854,711-16,881,987
(GRCh38/hg38)
Size:
27,277 bases
Orientation:
Minus strand
chr7:16,899,029-16,921,613
(GRCh37/hg19)
Size:
22,585 bases
Orientation:
Minus strand

Genomic View for AGR3 Gene

Genes around AGR3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
AGR3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for AGR3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for AGR3 Gene

Proteins for AGR3 Gene

  • Protein details for AGR3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8TD06-AGR3_HUMAN
    Recommended name:
    Anterior gradient protein 3
    Protein Accession:
    Q8TD06
    Secondary Accessions:
    • A4D120

    Protein attributes for AGR3 Gene

    Size:
    166 amino acids
    Molecular mass:
    19171 Da
    Quaternary structure:
    • Interacts with LYPD3 and DAG1 (alphaDAG1).

    Three dimensional structures from OCA and Proteopedia for AGR3 Gene

neXtProt entry for AGR3 Gene

Post-translational modifications for AGR3 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for AGR3 Gene

Protein Products

  • Abcam proteins for AGR3

No data available for DME Specific Peptides for AGR3 Gene

Domains & Families for AGR3 Gene

Gene Families for AGR3 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for AGR3 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for AGR3 Gene

GenScript: Design optimal peptide antigens:
  • Breast cancer membrane protein 11 (AGR3_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q8TD06

UniProtKB/Swiss-Prot:

AGR3_HUMAN :
  • Belongs to the AGR family.
Family:
  • Belongs to the AGR family.
genes like me logo Genes that share domains with AGR3: view

Function for AGR3 Gene

Molecular function for AGR3 Gene

UniProtKB/Swiss-Prot Function:
Required for calcium-mediated regulation of ciliary beat frequency and mucociliary clearance in the airway. Might be involved in the regulation of intracellular calcium in tracheal epithelial cells.
UniProtKB/Swiss-Prot Induction:
Not induced as part of the cellular response to endoplasmic reticulum stress (PubMed:25751668). Up-regulated by androgens and by estrogens in prostate cancer cells (PubMed:23294566) Up-regulated by a hormone (estrogen-receptor alpha) independent mechanism in ovarian cancer (PubMed:22361111).

Phenotypes From GWAS Catalog for AGR3 Gene

Gene Ontology (GO) - Molecular Function for AGR3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002162 dystroglycan binding IBA,IDA 12592373
GO:0005515 protein binding IPI 12592373
genes like me logo Genes that share ontologies with AGR3: view
genes like me logo Genes that share phenotypes with AGR3: view

Animal Model Products

  • Taconic Biosciences Mouse Models for AGR3

CRISPR Products

miRNA for AGR3 Gene

miRTarBase miRNAs that target AGR3

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for AGR3

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for AGR3 Gene

Localization for AGR3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for AGR3 Gene

Endoplasmic reticulum. Note=Found in the cytoplasm, which could include the endoplasmic reticulum. {ECO:0000305 PubMed:26170690}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for AGR3 gene
Compartment Confidence
endoplasmic reticulum 5
extracellular 3
plasma membrane 1
cytoskeleton 1
mitochondrion 1
nucleus 1
endosome 1
cytosol 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Endoplasmic reticulum (3)
  • Vesicles (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for AGR3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005783 endoplasmic reticulum IDA --
GO:0043231 intracellular membrane-bounded organelle IDA --
genes like me logo Genes that share ontologies with AGR3: view

Pathways & Interactions for AGR3 Gene

PathCards logo

SuperPathways for AGR3 Gene

No Data Available

Gene Ontology (GO) - Biological Process for AGR3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0008150 biological_process ND --
GO:0060548 negative regulation of cell death IBA 21873635
genes like me logo Genes that share ontologies with AGR3: view

No data available for Pathways by source and SIGNOR curated interactions for AGR3 Gene

Drugs & Compounds for AGR3 Gene

No Compound Related Data Available

Transcripts for AGR3 Gene

mRNA/cDNA for AGR3 Gene

1 REFSEQ mRNAs :
7 NCBI additional mRNA sequence :
4 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for AGR3

Alternative Splicing Database (ASD) splice patterns (SP) for AGR3 Gene

No ASD Table

Relevant External Links for AGR3 Gene

GeneLoc Exon Structure for
AGR3

Expression for AGR3 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for AGR3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for AGR3 Gene

This gene is overexpressed in Colon - Transverse (x17.0), Small Intestine - Terminal Ileum (x10.4), and Lung (x7.7).

Protein differential expression in normal tissues from HIPED for AGR3 Gene

This gene is overexpressed in Nasal epithelium (23.4), Oral epithelium (20.2), and Breast (12.8).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for AGR3 Gene



Protein tissue co-expression partners for AGR3 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for AGR3

SOURCE GeneReport for Unigene cluster for AGR3 Gene:

Hs.100686

mRNA Expression by UniProt/SwissProt for AGR3 Gene:

Q8TD06-AGR3_HUMAN
Tissue specificity: Expressed in the lung, in the ciliated cells of the airway epithelium (PubMed:25751668). Expression increased with differentiation of airway epithelial cells (PubMed:25751668). Not detected in the mucous cells (PubMed:25751668). Expressed in ciliated cells in the oviduct (PubMed:26170690). Also detected in stomach, colon, prostate and liver (PubMed:25751668). Expressed in breast, ovary, prostate and liver cancer (PubMed:26170690). Expression is associated with the level of differentiation of breast cancer (at protein level) (PubMed:26170690).

Evidence on tissue expression from TISSUES for AGR3 Gene

  • Lung(4.5)
  • Stomach(2.3)
  • Intestine(2.1)
genes like me logo Genes that share expression patterns with AGR3: view

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for AGR3 Gene

Orthologs for AGR3 Gene

This gene was present in the common ancestor of animals.

Orthologs for AGR3 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia AGR3 30 31
  • 98.8 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia AGR3 30 31
  • 88.96 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Agr3 30
  • 86.06 (n)
Mouse
(Mus musculus)
Mammalia Agr3 30 17 31
  • 85.86 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia AGR3 31
  • 60 (a)
OneToOne
Chicken
(Gallus gallus)
Aves AGR3 30 31
  • 78.59 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia AGR3 31
  • 78 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia agr3 30
  • 74.25 (n)
Str.8536 30
Zebrafish
(Danio rerio)
Actinopterygii zgc:165571 31
  • 59 (a)
ManyToMany
Rainbow Trout
(Oncorhynchus mykiss)
Actinopterygii Omy.9044 30
Worm
(Caenorhabditis elegans)
Secernentea Y57A10A.23 31
  • 25 (a)
ManyToMany
F49H12.5 31
  • 16 (a)
ManyToMany
Species where no ortholog for AGR3 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • Dog (Canis familiaris)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Platypus (Ornithorhynchus anatinus)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for AGR3 Gene

ENSEMBL:
Gene Tree for AGR3 (if available)
TreeFam:
Gene Tree for AGR3 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for AGR3: view image

Paralogs for AGR3 Gene

Paralogs for AGR3 Gene

(2) SIMAP similar genes for AGR3 Gene using alignment to 3 proteins:

  • AGR3_HUMAN
  • B5MC62_HUMAN
  • H7C040_HUMAN
genes like me logo Genes that share paralogs with AGR3: view

Variants for AGR3 Gene

Additional dbSNP identifiers (rs#s) for AGR3 Gene

Structural Variations from Database of Genomic Variants (DGV) for AGR3 Gene

Variant ID Type Subtype PubMed ID
dgv11225n54 CNV gain 21841781
dgv1273e214 CNV gain 21293372
dgv6289n100 CNV gain 25217958
dgv6290n100 CNV gain 25217958
esv2752152 CNV loss 17911159
esv2761305 CNV loss 21179565
esv3571672 CNV loss 25503493
esv3612405 CNV loss 21293372
esv3891076 CNV gain 25118596
nsv1027284 CNV loss 25217958
nsv464398 CNV gain 19166990
nsv522109 CNV gain 19592680
nsv522500 CNV loss 19592680
nsv606339 CNV loss 21841781

Variation tolerance for AGR3 Gene

Residual Variation Intolerance Score: 54.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.56; 12.10% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for AGR3 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
AGR3

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for AGR3 Gene

Disorders for AGR3 Gene

MalaCards: The human disease database

(5) MalaCards diseases for AGR3 Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
breast abscess
  • abscess of breast
ritter's disease
  • dermatitis exfoliativa neonatorum
breast cancer
  • breast lobular carcinoma
ovarian cancer
  • ovarian cancer, somatic
prostate cancer
  • prostate cancer, somatic
- elite association - COSMIC cancer census association via MalaCards
Search AGR3 in MalaCards View complete list of genes associated with diseases
genes like me logo Genes that share disorders with AGR3: view

No data available for UniProtKB/Swiss-Prot and Genatlas for AGR3 Gene

Publications for AGR3 Gene

  1. hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan. (PMID: 12592373) Fletcher GC … Terrett JA (British journal of cancer 2003) 2 3 4 23
  2. The Endoplasmic Reticulum Resident Protein AGR3. Required for Regulation of Ciliary Beat Frequency in the Airway. (PMID: 25751668) Bonser LR … Erle DJ (American journal of respiratory cell and molecular biology 2015) 3 4
  3. Anterior Gradient-3: a novel biomarker for ovarian cancer that mediates cisplatin resistance in xenograft models. (PMID: 22361111) Gray TA … Hupp TR (Journal of immunological methods 2012) 3 4
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4
  5. Signal peptide prediction based on analysis of experimentally verified cleavage sites. (PMID: 15340161) Zhang Z … Henzel WJ (Protein science : a publication of the Protein Society 2004) 3 4

Products for AGR3 Gene

Sources for AGR3 Gene