This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic tr... See more...

Aliases for AGO3 Gene

Aliases for AGO3 Gene

  • Argonaute RISC Catalytic Component 3 2 3 4 5
  • Eukaryotic Translation Initiation Factor 2C, 3 2 3
  • Protein Argonaute-3 3 4
  • EIF2C3 3 4
  • HAgo3 3 4
  • Eukaryotic Translation Initiation Factor 2C 3 4
  • Argonaute 3, RISC Catalytic Component 3
  • EC 3.1.26.n2 4
  • Argonaute 3 2
  • Argonaute3 4
  • FLJ12765 2
  • EIF-2C 3 4
  • EIF2C 3 4
  • HAGO3 2
  • AGO3 5

External Ids for AGO3 Gene

Previous HGNC Symbols for AGO3 Gene

  • EIF2C3

Previous GeneCards Identifiers for AGO3 Gene

  • GC01P035452
  • GC01P036397
  • GC01P036399
  • GC01P036402
  • GC01P035930
  • GC01P035923

Summaries for AGO3 Gene

Entrez Gene Summary for AGO3 Gene

  • This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

GeneCards Summary for AGO3 Gene

AGO3 (Argonaute RISC Catalytic Component 3) is a Protein Coding gene. Among its related pathways are RET signaling and Signaling by GPCR. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and RNA binding. An important paralog of this gene is AGO1.

UniProtKB/Swiss-Prot Summary for AGO3 Gene

  • Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity (PubMed:29040713).

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for AGO3 Gene

Genomics for AGO3 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for AGO3 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH01J035929 Promoter/Enhancer 2.3 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas 623.1 +5.5 5518 4 SP1 PRDM10 ZNF629 SIX5 LEF1 ZNF692 POLR2A BACH1 ETV6 JUND AGO3 ENSG00000271914 piR-38945 ZMYM4 SFPQ ZMYM6 LOC653160 C1orf216 TEKT2 THRAP3
GH01J035924 Enhancer 0.5 Ensembl 608.8 -0.7 -682 0.8 PKNOX1 RELB POLR2A RUNX3 TBX21 MTA2 SPI1 AGO3 lnc-AGO3-2 piR-40027 ENSG00000271554 piR-46031 AGO1
GH01J036154 Promoter/Enhancer 2.8 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER 74.7 +235.5 235517 14 ARHGAP35 SP1 GATAD2A CTCF PRDM10 ZNF629 LEF1 IKZF1 SMAD2 KDM1A MAP7D1 SFPQ THRAP3 ZMYM4 RPL5P4 AGO3 EVA1B COL8A2 CSF3R ENSG00000271554
GH01J035925 Enhancer 0.3 Ensembl 600.7 +0.2 218 0.2 FOXA1 POLR2A AGO3 lnc-AGO3-2 piR-40027 AGO1 TEKT2
GH01J035947 Promoter 0.3 EPDnew 600.4 +21.7 21704 0.1 AGO3 ENSG00000271914 lnc-AGO1-5 TEKT2
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around AGO3 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for AGO3

Genomic Locations for AGO3 Gene

Genomic Locations for AGO3 Gene
chr1:35,925,683-36,072,500
(GRCh38/hg38)
Size:
146,818 bases
Orientation:
Plus strand
chr1:36,396,319-36,538,101
(GRCh37/hg19)
Size:
141,783 bases
Orientation:
Plus strand

Genomic View for AGO3 Gene

Genes around AGO3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
AGO3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for AGO3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for AGO3 Gene

Proteins for AGO3 Gene

  • Protein details for AGO3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9H9G7-AGO3_HUMAN
    Recommended name:
    Protein argonaute-3
    Protein Accession:
    Q9H9G7
    Secondary Accessions:
    • B1ALI0
    • Q5TA55
    • Q9H1U6

    Protein attributes for AGO3 Gene

    Size:
    860 amino acids
    Molecular mass:
    97360 Da
    Quaternary structure:
    • Interacts with EIF4B, IMP8, PRMT5 and TNRC6B. Interacts with APOBEC3F, APOBEC3G and APOBEC3H. Interacts with EDC4.

    Three dimensional structures from OCA and Proteopedia for AGO3 Gene

    Alternative splice isoforms for AGO3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for AGO3 Gene

Post-translational modifications for AGO3 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for AGO3 Gene

Domains & Families for AGO3 Gene

Gene Families for AGO3 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for AGO3 Gene

InterPro:
Blocks:
  • Argonaute and Dicer protein, PAZ
  • Stem cell self-renewal protein Piwi
ProtoNet:

Suggested Antigen Peptide Sequences for AGO3 Gene

GenScript: Design optimal peptide antigens:
  • Eukaryotic translation initiation factor 2C 3 (AGO3_HUMAN)
  • Eukaryotic translation initiation factor 2C, 3 (B1ALI0_HUMAN)
  • EIF2C3 protein (Q6NXU1_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9H9G7

UniProtKB/Swiss-Prot:

AGO3_HUMAN :
  • Belongs to the argonaute family. Ago subfamily.
Family:
  • Belongs to the argonaute family. Ago subfamily.
genes like me logo Genes that share domains with AGO3: view

Function for AGO3 Gene

Molecular function for AGO3 Gene

UniProtKB/Swiss-Prot Function:
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity (PubMed:29040713).
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=Endonucleolytic cleavage to 5'-phosphomonoester.; EC=3.1.26.n2; Evidence={ECO:0000269|PubMed:29040713};.

Enzyme Numbers (IUBMB) for AGO3 Gene

Phenotypes From GWAS Catalog for AGO3 Gene

Gene Ontology (GO) - Molecular Function for AGO3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003676 nucleic acid binding IEA --
GO:0003723 RNA binding IEA,HDA 22658674
GO:0003725 double-stranded RNA binding IDA 19966796
GO:0003727 single-stranded RNA binding IDA 19966796
GO:0003743 translation initiation factor activity IEA --
genes like me logo Genes that share ontologies with AGO3: view
genes like me logo Genes that share phenotypes with AGO3: view

Animal Models for AGO3 Gene

MGI Knock Outs for AGO3:
  • Ago3 Ago3<tm1a(KOMP)Wtsi>

Animal Model Products

CRISPR Products

miRNA for AGO3 Gene

miRTarBase miRNAs that target AGO3

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for AGO3

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for AGO3 Gene

Localization for AGO3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for AGO3 Gene

Cytoplasm, P-body.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for AGO3 gene
Compartment Confidence
nucleus 5
cytosol 5
mitochondrion 2
plasma membrane 1
extracellular 1
cytoskeleton 1
endoplasmic reticulum 1
endosome 1
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytoplasmic bodies (3)
  • Nucleoplasm (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for AGO3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000794 condensed nuclear chromosome IEA --
GO:0000932 P-body IEA --
GO:0005654 nucleoplasm IDA --
GO:0005737 cytoplasm IEA,IDA 15260970
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with AGO3: view

Pathways & Interactions for AGO3 Gene

genes like me logo Genes that share pathways with AGO3: view

Pathways by source for AGO3 Gene

Gene Ontology (GO) - Biological Process for AGO3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006402 mRNA catabolic process IDA 18771919
GO:0006413 translational initiation IEA --
GO:0006417 regulation of translation IEA --
GO:0007223 Wnt signaling pathway, calcium modulating pathway TAS --
GO:0010501 RNA secondary structure unwinding IMP 22795694
genes like me logo Genes that share ontologies with AGO3: view

No data available for SIGNOR curated interactions for AGO3 Gene

Drugs & Compounds for AGO3 Gene

No Compound Related Data Available

Transcripts for AGO3 Gene

mRNA/cDNA for AGO3 Gene

2 REFSEQ mRNAs :
12 NCBI additional mRNA sequence :
7 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for AGO3

Alternative Splicing Database (ASD) splice patterns (SP) for AGO3 Gene

ExUns: 1 ^ 2a · 2b · 2c · 2d ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19 ^ 20 ^ 21a ·
SP1: - - - - -
SP2: - - - - - - -
SP3: - -
SP4: - -
SP5:
SP6:

ExUns: 21b ^ 22a · 22b ^ 23
SP1: -
SP2: -
SP3:
SP4:
SP5:
SP6:

Relevant External Links for AGO3 Gene

GeneLoc Exon Structure for
AGO3

Expression for AGO3 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for AGO3 Gene

Protein differential expression in normal tissues from HIPED for AGO3 Gene

This gene is overexpressed in Uterus (6.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for AGO3 Gene



Protein tissue co-expression partners for AGO3 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for AGO3

SOURCE GeneReport for Unigene cluster for AGO3 Gene:

Hs.657659

Evidence on tissue expression from TISSUES for AGO3 Gene

  • Blood(4.4)
  • Nervous system(2.3)
  • Kidney(2.2)
  • Liver(2.1)
genes like me logo Genes that share expression patterns with AGO3: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for AGO3 Gene

Orthologs for AGO3 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for AGO3 Gene

Organism Taxonomy Gene Similarity Type Details
Platypus
(Ornithorhynchus anatinus)
Mammalia AGO3 31
  • 100 (a)
OneToOne
Chimpanzee
(Pan troglodytes)
Mammalia EIF2C3 30 31
  • 99.77 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia AGO3 31
  • 99 (a)
OneToOne
Dog
(Canis familiaris)
Mammalia AGO3 30 31
  • 95.85 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia AGO3 30 31
  • 95.16 (n)
OneToOne
Mouse
(Mus musculus)
Mammalia Ago3 30 17 31
  • 92.6 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Ago3 30
  • 91.43 (n)
Chicken
(Gallus gallus)
Aves AGO3 30 31
  • 87.52 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia AGO3 31
  • 99 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia ago3 30
  • 83.33 (n)
Zebrafish
(Danio rerio)
Actinopterygii AGO3 (1 of 2) 31
  • 97 (a)
OneToMany
ago3b 30 31
  • 77.48 (n)
OneToMany
Thale Cress
(Arabidopsis thaliana)
eudicotyledons AGO1 30
  • 53.83 (n)
Rice
(Oryza sativa)
Liliopsida Os02g0672200 30
  • 53.11 (n)
Os.10753 30
Species where no ortholog for AGO3 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for AGO3 Gene

ENSEMBL:
Gene Tree for AGO3 (if available)
TreeFam:
Gene Tree for AGO3 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for AGO3: view image

Paralogs for AGO3 Gene

Paralogs for AGO3 Gene

(5) SIMAP similar genes for AGO3 Gene using alignment to 2 proteins:

  • AGO3_HUMAN
  • Q5TA56_HUMAN
genes like me logo Genes that share paralogs with AGO3: view

Variants for AGO3 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for AGO3 Gene

SNP ID Clinical significance and condition Chr 01 pos Variation AA Info Type
717673 Likely Benign: not provided 36,036,228(+) T/C SYNONYMOUS_VARIANT
720219 Likely Benign: not provided 36,008,945(+) G/A SYNONYMOUS_VARIANT
720252 Benign: not provided 36,009,017(+) A/G SYNONYMOUS_VARIANT
723872 Benign: not provided 35,972,221(+) G/A SYNONYMOUS_VARIANT,FIVE_PRIME_UTR_VARIANT
726237 Benign: not provided 36,034,242(+) A/G MISSENSE_VARIANT

Additional dbSNP identifiers (rs#s) for AGO3 Gene

Structural Variations from Database of Genomic Variants (DGV) for AGO3 Gene

Variant ID Type Subtype PubMed ID
dgv149n100 CNV loss 25217958
esv3305175 CNV mobile element insertion 20981092
esv3309407 CNV mobile element insertion 20981092
esv3335594 CNV insertion 20981092
esv3371096 CNV insertion 20981092
esv3435241 CNV insertion 20981092
esv3585726 CNV gain 21293372
esv3585728 CNV loss 21293372
nsv1009033 CNV loss 25217958
nsv1009992 CNV gain+loss 25217958
nsv159158 CNV insertion 16902084
nsv518844 CNV loss 19592680
nsv998713 CNV gain 25217958

Variation tolerance for AGO3 Gene

Residual Variation Intolerance Score: 10.2% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.42; 9.12% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for AGO3 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
AGO3

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for AGO3 Gene

Disorders for AGO3 Gene

No disorders were found for AGO3 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for AGO3 Gene

Publications for AGO3 Gene

  1. Human Argonaute3 has slicer activity. (PMID: 29040713) Park MS … Nakanishi K (Nucleic acids research 2017) 2 3 4
  2. DICER- and AGO3-dependent generation of retinoic acid-induced DR2 Alu RNAs regulates human stem cell proliferation. (PMID: 23064648) Hu Q … Rosenfeld MG (Nature structural & molecular biology 2012) 3 4
  3. HIV-1 replication and APOBEC3 antiviral activity are not regulated by P bodies. (PMID: 22915799) Phalora PK … Malim MH (Journal of virology 2012) 3 4
  4. Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs. (PMID: 19167051) Weinmann L … Meister G (Cell 2009) 3 4
  5. Importance of translation and nonnucleolytic ago proteins for on-target RNA interference. (PMID: 18771919) Wu L … Belasco JG (Current biology : CB 2008) 3 4

Products for AGO3 Gene

Sources for AGO3 Gene