This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The express... See more...

Aliases for ADAM21 Gene

Aliases for ADAM21 Gene

  • ADAM Metallopeptidase Domain 21 2 3 5
  • Disintegrin And Metalloproteinase Domain-Containing Protein 21 3 4
  • A Disintegrin And Metalloproteinase Domain 21 2 3
  • ADAM 21 3 4
  • ADAM Metallopeptidase Domain 21, Preproprotein 3
  • EC 3.4.24.- 4
  • ADAM31 3

External Ids for ADAM21 Gene

Previous GeneCards Identifiers for ADAM21 Gene

  • GC14P068429
  • GC14P064720
  • GC14P068914
  • GC14P068688
  • GC14P069993
  • GC14P070924

Summaries for ADAM21 Gene

Entrez Gene Summary for ADAM21 Gene

  • This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The expression of this gene expression is testis-specific. [provided by RefSeq, May 2011]

GeneCards Summary for ADAM21 Gene

ADAM21 (ADAM Metallopeptidase Domain 21) is a Protein Coding gene. Among its related pathways are Fertilization. Gene Ontology (GO) annotations related to this gene include metalloendopeptidase activity and metallopeptidase activity. An important paralog of this gene is ADAM20.

UniProtKB/Swiss-Prot Summary for ADAM21 Gene

  • May be involved in sperm maturation and/or fertilization. May also be involved in epithelia functions associated with establishing and maintaining gradients of ions or nutrients.

Additional gene information for ADAM21 Gene

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for ADAM21 Gene

Genomics for ADAM21 Gene

GeneHancer (GH) Regulatory Elements for ADAM21 Gene

Promoters and enhancers for ADAM21 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH14J069715 Enhancer 1.2 FANTOM5 ENCODE dbSUPER 26 -735.0 -735029 2.9 ZNF300 ZNF10 ZIC2 ZNF662 ATF2 OSR2 ZNF491 CTCF NFIC ZNF195 SRSF5 EXD2 ADAM21 ERH SUSD6 ADAM20 ADAM20P1 ENSG00000258957 piR-33294
GH14J070317 Enhancer 0.9 ENCODE 5.1 -133.9 -133931 1.7 EP300 FOXK2 CTCF NCOR1 KDM6A TCF7 REST ZBTB25 TGIF2 RAD21 COX16 RNU2-51P SLC8A3 ADAM20P1 ADAM21 NONHSAG015344.2 piR-59065 ENSG00000259033
GH14J070212 Enhancer 0.4 Ensembl 10.1 -239.3 -239256 0.2 ZIC2 ATF2 ATF7 SLC10A1 ADAM21 piR-38351-156 SLC8A3 piR-56883-065 ENSG00000258422
GH14J070401 Enhancer 0.7 Ensembl ENCODE 4.5 -49.3 -49258 2.4 CTCF NFIC SMC3 SRF RAD21 BHLHE40 KDM1A COX16 ADAM21 lnc-SYNJ2BP-2 SYNJ2BP SYNJ2BP-COX16
GH14J070278 Enhancer 0.4 Ensembl 6.2 -171.7 -171656 4.6 RFX5 SPI1 ZSCAN21 COX16 ADAM21P1 RNU2-51P ADAM21 ADAM20P1 SLC8A3 SYNJ2BP piR-35011-004 piR-36588-158 ENSG00000259033
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around ADAM21 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for ADAM21

Top Transcription factor binding sites by QIAGEN in the ADAM21 gene promoter:
  • C/EBPalpha
  • CHOP-10
  • E47
  • Hand1
  • Hlf
  • Pbx1a
  • POU2F1
  • POU2F1a
  • RORalpha2

Genomic Locations for ADAM21 Gene

Genomic Locations for ADAM21 Gene
chr14:70,452,157-70,459,905
(GRCh38/hg38)
Size:
7,749 bases
Orientation:
Plus strand
chr14:70,918,874-70,926,622
(GRCh37/hg19)
Size:
7,749 bases
Orientation:
Plus strand

Genomic View for ADAM21 Gene

Genes around ADAM21 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ADAM21 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ADAM21 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ADAM21 Gene

Proteins for ADAM21 Gene

  • Protein details for ADAM21 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9UKJ8-ADA21_HUMAN
    Recommended name:
    Disintegrin and metalloproteinase domain-containing protein 21
    Protein Accession:
    Q9UKJ8
    Secondary Accessions:
    • O43507
    • Q2VPC6
    • Q32MR0

    Protein attributes for ADAM21 Gene

    Size:
    722 amino acids
    Molecular mass:
    80834 Da
    Cofactor:
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
    Quaternary structure:
    No Data Available
    Miscellaneous:
    • May be the functional equivalent of ADAM 1/fertilin alpha which is a pseudogene in human.

neXtProt entry for ADAM21 Gene

Post-translational modifications for ADAM21 Gene

  • Has no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated.
  • Glycosylation at Asn164, Asn227, Asn377, Asn437, Asn478, Asn546, and Asn600
  • Modification sites at PhosphoSitePlus

Other Protein References for ADAM21 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for ADAM21 Gene

Domains & Families for ADAM21 Gene

Gene Families for ADAM21 Gene

Protein Domains for ADAM21 Gene

Suggested Antigen Peptide Sequences for ADAM21 Gene

GenScript: Design optimal peptide antigens:
  • Disintegrin and metalloproteinase domain-containing protein 21 (ADA21_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9UKJ8

UniProtKB/Swiss-Prot:

ADA21_HUMAN :
  • A tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
Domain:
  • A tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
  • The cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion.
  • The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
genes like me logo Genes that share domains with ADAM21: view

Function for ADAM21 Gene

Molecular function for ADAM21 Gene

UniProtKB/Swiss-Prot Function:
May be involved in sperm maturation and/or fertilization. May also be involved in epithelia functions associated with establishing and maintaining gradients of ions or nutrients.
GENATLAS Biochemistry:
a disintegrin and metalloprotease (active) domain 21,membrane anchored cell surface adhesion protein,testis specific with similarity to fertilin-alpha

Enzyme Numbers (IUBMB) for ADAM21 Gene

Phenotypes From GWAS Catalog for ADAM21 Gene

Gene Ontology (GO) - Molecular Function for ADAM21 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004222 metalloendopeptidase activity IEA --
GO:0008233 peptidase activity IEA --
GO:0008237 metallopeptidase activity TAS 9469942
GO:0016787 hydrolase activity IEA --
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with ADAM21: view
genes like me logo Genes that share phenotypes with ADAM21: view

Animal Models for ADAM21 Gene

MGI Knock Outs for ADAM21:

Animal Model Products

  • Taconic Biosciences Mouse Models for ADAM21

CRISPR Products

miRNA for ADAM21 Gene

miRTarBase miRNAs that target ADAM21

Clone Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for ADAM21 Gene

Localization for ADAM21 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ADAM21 Gene

Membrane; Single-pass type I membrane protein.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ADAM21 gene
Compartment Confidence
plasma membrane 5
extracellular 1
cytoskeleton 1
nucleus 1
endoplasmic reticulum 1
peroxisome 0

Gene Ontology (GO) - Cellular Components for ADAM21 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005886 plasma membrane TAS --
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane IEA --
GO:0030424 axon IEA --
GO:0043005 neuron projection IEA --
genes like me logo Genes that share ontologies with ADAM21: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for ADAM21 Gene

Pathways & Interactions for ADAM21 Gene

PathCards logo

SuperPathways for ADAM21 Gene

SuperPathway Contained pathways
1 Fertilization
genes like me logo Genes that share pathways with ADAM21: view

Pathways by source for ADAM21 Gene

3 Reactome pathways for ADAM21 Gene

Gene Ontology (GO) - Biological Process for ADAM21 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006508 proteolysis IEA --
GO:0007338 single fertilization TAS 9469942
GO:0007339 binding of sperm to zona pellucida TAS --
genes like me logo Genes that share ontologies with ADAM21: view

No data available for SIGNOR curated interactions for ADAM21 Gene

Drugs & Compounds for ADAM21 Gene

No Compound Related Data Available

Transcripts for ADAM21 Gene

mRNA/cDNA for ADAM21 Gene

1 REFSEQ mRNAs :
5 NCBI additional mRNA sequence :
1 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for ADAM21 Gene

No ASD Table

Relevant External Links for ADAM21 Gene

GeneLoc Exon Structure for
ADAM21

Expression for ADAM21 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for ADAM21 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for ADAM21 Gene

This gene is overexpressed in Testis (x22.0).

Protein differential expression in normal tissues from HIPED for ADAM21 Gene

This gene is overexpressed in Heart (57.7) and Testis (11.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for ADAM21 Gene



Protein tissue co-expression partners for ADAM21 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for ADAM21

SOURCE GeneReport for Unigene cluster for ADAM21 Gene:

Hs.178748
genes like me logo Genes that share expression patterns with ADAM21: view

No data available for mRNA Expression by UniProt/SwissProt , Evidence on tissue expression from TISSUES and Phenotype-based relationships between genes and organs from Gene ORGANizer for ADAM21 Gene

Orthologs for ADAM21 Gene

This gene was present in the common ancestor of animals.

Orthologs for ADAM21 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia ADAM21 31 30
  • 98.89 (n)
OneToOne
dog
(Canis familiaris)
Mammalia ADAM21 31 30
  • 81.92 (n)
OneToOne
cow
(Bos Taurus)
Mammalia ADAM21 31 30
  • 80.72 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Adam21 17 31 30
  • 78.14 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia -- 31
  • 45 (a)
OneToMany
oppossum
(Monodelphis domestica)
Mammalia -- 31
  • 35 (a)
OneToMany
chicken
(Gallus gallus)
Aves LOC100857840 30
  • 52.36 (n)
-- 31
  • 40 (a)
OneToMany
lizard
(Anolis carolinensis)
Reptilia -- 31
  • 42 (a)
ManyToMany
-- 31
  • 41 (a)
ManyToMany
-- 31
  • 40 (a)
ManyToMany
-- 31
  • 40 (a)
ManyToMany
-- 31
  • 40 (a)
ManyToMany
-- 31
  • 39 (a)
ManyToMany
zebrafish
(Danio rerio)
Actinopterygii zgc:174164 31
  • 28 (a)
ManyToMany
fruit fly
(Drosophila melanogaster)
Insecta Neu3 31
  • 15 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea adm-2 31
  • 19 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 32 (a)
ManyToMany
-- 31
  • 31 (a)
ManyToMany
Species where no ortholog for ADAM21 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rat (Rattus norvegicus)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)

Evolution for ADAM21 Gene

ENSEMBL:
Gene Tree for ADAM21 (if available)
TreeFam:
Gene Tree for ADAM21 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for ADAM21: view image

Paralogs for ADAM21 Gene

(13) SIMAP similar genes for ADAM21 Gene using alignment to 1 proteins:

  • ADA21_HUMAN
genes like me logo Genes that share paralogs with ADAM21: view

Variants for ADAM21 Gene

Additional dbSNP identifiers (rs#s) for ADAM21 Gene

Structural Variations from Database of Genomic Variants (DGV) for ADAM21 Gene

Variant ID Type Subtype PubMed ID
esv3581280 CNV loss 25503493
nsv1116461 OTHER inversion 24896259
nsv976352 CNV duplication 23825009

Variation tolerance for ADAM21 Gene

Residual Variation Intolerance Score: 96.5% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 7.19; 80.88% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ADAM21 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
ADAM21

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for ADAM21 Gene

Disorders for ADAM21 Gene

Additional Disease Information for ADAM21

No disorders were found for ADAM21 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for ADAM21 Gene

Publications for ADAM21 Gene

  1. ADAM 20 and 21; two novel human testis-specific membrane metalloproteases with similarity to fertilin-alpha. (PMID: 9469942) Hooft van Huijsduijnen R (Gene 1998) 2 3 4 54
  2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 54
  3. The identification of seven metalloproteinase-disintegrin (ADAM) genes from genomic libraries. (PMID: 10524237) Poindexter K … Cerretti DP (Gene 1999) 3 4 54
  4. Architecture of the human interactome defines protein communities and disease networks. (PMID: 28514442) Huttlin EL … Harper JW (Nature 2017) 3 54
  5. Overexpression of a disintegrin and metalloproteinase 21 is associated with motility, metastasis, and poor prognosis in hepatocellular carcinoma. (PMID: 29138461) Honda H … Terai S (Scientific reports 2017) 3 54

Products for ADAM21 Gene

Sources for ADAM21 Gene