This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The express... See more...

Aliases for ADAM20 Gene

Aliases for ADAM20 Gene

  • ADAM Metallopeptidase Domain 20 2 3 5
  • Disintegrin And Metalloproteinase Domain-Containing Protein 20 3 4
  • A Disintegrin And Metalloproteinase Domain 20 2 3
  • ADAM 20 3 4
  • EC 3.4.24.58 50
  • EC 3.4.24.- 4
  • EC 3.4.24 50
  • ADAM20 5

External Ids for ADAM20 Gene

Previous GeneCards Identifiers for ADAM20 Gene

  • GC14M068494
  • GC14M064785
  • GC14M068979
  • GC14M070058
  • GC14M070989
  • GC14M051157

Summaries for ADAM20 Gene

Entrez Gene Summary for ADAM20 Gene

  • This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The expression of this gene is testis-specific. [provided by RefSeq, Jul 2008]

GeneCards Summary for ADAM20 Gene

ADAM20 (ADAM Metallopeptidase Domain 20) is a Protein Coding gene. Among its related pathways are Reproduction. Gene Ontology (GO) annotations related to this gene include metalloendopeptidase activity and metallopeptidase activity. An important paralog of this gene is ADAM21.

UniProtKB/Swiss-Prot Summary for ADAM20 Gene

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for ADAM20 Gene

Genomics for ADAM20 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for ADAM20 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH14J070534 Promoter 0.4 EPDnew 250.3 +29.7 29727 0.1 ATF2 piR-38352-180 ADAM20 piR-56497-047 ENSG00000257759 LOC105370556 ADAM21
GH14J069995 Enhancer 1.5 FANTOM5 Ensembl ENCODE dbSUPER 11.6 +566.4 566377 6.7 ZNF600 ZNF580 ATF2 CEBPA ATF3 JUND BRCA1 ZIC2 ZBTB10 CEBPB SMOC1 MED6 ADAM20 SRSF5 DCAF5 ERH SLC10A1 TTC9 HSALNG0102198 lnc-SLC10A1-2
GH14J069715 Enhancer 1.2 FANTOM5 ENCODE dbSUPER 10.3 +847.6 847614 2.9 ZNF600 ATF2 POLR2A ZNF10 ZIC2 NFIC ZNF513 ZNF610 EBF1 BCL11B HSALNG0102186 SRSF5 EXD2 ERH ADAM21 SUSD6 ADAM20 ADAM20P1 piR-33294
GH14J070254 Promoter/Enhancer 1.5 Ensembl ENCODE CraniofacialAtlas 5.4 +309.3 309341 2.4 ZNF600 POLR2A NFIC REST HOMEZ KMT2B ELF1 KLF9 MAFF SMAD4 ENSG00000259033 LOC101928046 piR-35564-045 piR-33735-026 piR-58297-163 COX16 SRSF5 SLC8A3 RNU2-51P ADAM20
GH14J070010 Enhancer 0.6 Ensembl dbSUPER 4.2 +554.2 554241 1.4 ZIC2 FEZF1 RFX5 SMOC1 ADAM20 HSALNG0102198 lnc-SLC10A1-2 SLC8A3
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around ADAM20 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for ADAM20

Top Transcription factor binding sites by QIAGEN in the ADAM20 gene promoter:
  • MIF-1
  • RFX1
  • ZID

Genomic Locations for ADAM20 Gene

Latest Assembly
chr14:70,520,817-70,564,742
(GRCh38/hg38)
Size:
43,926 bases
Orientation:
Minus strand

Previous Assembly
chr14:70,989,075-71,001,721
(GRCh37/hg19 by Entrez Gene)
Size:
12,647 bases
Orientation:
Minus strand

chr14:70,989,075-71,001,732
(GRCh37/hg19 by Ensembl)
Size:
12,658 bases
Orientation:
Minus strand

Genomic View for ADAM20 Gene

Genes around ADAM20 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ADAM20 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ADAM20 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ADAM20 Gene

Proteins for ADAM20 Gene

  • Protein details for ADAM20 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O43506-ADA20_HUMAN
    Recommended name:
    Disintegrin and metalloproteinase domain-containing protein 20
    Protein Accession:
    O43506
    Secondary Accessions:
    • Q6GTZ1
    • Q9UKJ9

    Protein attributes for ADAM20 Gene

    Size:
    726 amino acids
    Molecular mass:
    81603 Da
    Cofactor:
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
    Quaternary structure:
    No Data Available
    SequenceCaution:
    • Sequence=AAH25378.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; Sequence=AAH25378.2; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=EAW81037.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
    Miscellaneous:
    • May be the functional equivalent of ADAM 1/fertilin alpha which is a pseudogene in human.

neXtProt entry for ADAM20 Gene

Selected DME Specific Peptides for ADAM20 Gene

O43506:
  • EEGEECDCG
  • GEECDCG
  • PEVVIPL
  • HELGHNLG

Post-translational modifications for ADAM20 Gene

  • Has no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated.
  • Glycosylation at Asn191, Asn226, Asn378, Asn438, Asn479, and Asn587
  • Modification sites at PhosphoSitePlus

Other Protein References for ADAM20 Gene

Domains & Families for ADAM20 Gene

Gene Families for ADAM20 Gene

Protein Domains for ADAM20 Gene

Suggested Antigen Peptide Sequences for ADAM20 Gene

GenScript: Design optimal peptide antigens:
  • Disintegrin and metalloproteinase domain-containing protein 20 (ADA20_HUMAN)

Graphical View of Domain Structure for InterPro Entry

O43506

UniProtKB/Swiss-Prot:

ADA20_HUMAN :
  • A tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
Domain:
  • A tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
  • The cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion.
  • The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
genes like me logo Genes that share domains with ADAM20: view

Function for ADAM20 Gene

Molecular function for ADAM20 Gene

UniProtKB/Swiss-Prot Function:
May be involved in sperm maturation and/or fertilization.
GENATLAS Biochemistry:
a disintegrin and metalloprotease (active) domain 20,membrane anchored cell surface adhesion protein,testis specific with similarity to fertilin-alpha

Enzyme Numbers (IUBMB) for ADAM20 Gene

Phenotypes From GWAS Catalog for ADAM20 Gene

Gene Ontology (GO) - Molecular Function for ADAM20 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004222 metalloendopeptidase activity IEA --
GO:0008233 peptidase activity IEA --
GO:0008237 metallopeptidase activity IEA,TAS 9469942
GO:0016787 hydrolase activity IEA --
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with ADAM20: view
genes like me logo Genes that share phenotypes with ADAM20: view

Animal Models for ADAM20 Gene

MGI Knock Outs for ADAM20:
  • Adam20 Adam20<tm1b(EUCOMM)Hmgu>

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ADAM20

No data available for Human Phenotype Ontology , miRNA , Transcription Factor Targets and HOMER Transcription for ADAM20 Gene

Localization for ADAM20 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ADAM20 Gene

Membrane. Single-pass type I membrane protein.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ADAM20 gene
Compartment Confidence
plasma membrane 4
extracellular 1
nucleus 1

Gene Ontology (GO) - Cellular Components for ADAM20 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005886 plasma membrane TAS --
GO:0009897 external side of plasma membrane IBA 21873635
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane IEA --
GO:1990913 sperm head plasma membrane IBA 21873635
genes like me logo Genes that share ontologies with ADAM20: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for ADAM20 Gene

Pathways & Interactions for ADAM20 Gene

PathCards logo

SuperPathways for ADAM20 Gene

SuperPathway Contained pathways
1 Reproduction
genes like me logo Genes that share pathways with ADAM20: view

Pathways by source for ADAM20 Gene

3 Reactome pathways for ADAM20 Gene

Gene Ontology (GO) - Biological Process for ADAM20 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006508 proteolysis IEA --
GO:0007338 single fertilization TAS 9469942
GO:0007339 binding of sperm to zona pellucida TAS --
GO:0008584 male gonad development IBA 21873635
genes like me logo Genes that share ontologies with ADAM20: view

No data available for SIGNOR curated interactions for ADAM20 Gene

Drugs & Compounds for ADAM20 Gene

No Compound Related Data Available

Transcripts for ADAM20 Gene

mRNA/cDNA for ADAM20 Gene

1 REFSEQ mRNAs :
4 NCBI additional mRNA sequence :
2 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ADAM20

Alternative Splicing Database (ASD) splice patterns (SP) for ADAM20 Gene

No ASD Table

Relevant External Links for ADAM20 Gene

GeneLoc Exon Structure for
ADAM20

Expression for ADAM20 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for ADAM20 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for ADAM20 Gene

This gene is overexpressed in Testis (x21.1).

Protein differential expression in normal tissues from HIPED for ADAM20 Gene

This gene is overexpressed in Cervix (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for ADAM20 Gene



Protein tissue co-expression partners for ADAM20 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for ADAM20

SOURCE GeneReport for Unigene cluster for ADAM20 Gene:

Hs.177984

mRNA Expression by UniProt/SwissProt for ADAM20 Gene:

O43506-ADA20_HUMAN
Tissue specificity: Testis specific.
genes like me logo Genes that share expression patterns with ADAM20: view

Primer products for research

No data available for Evidence on tissue expression from TISSUES and Phenotype-based relationships between genes and organs from Gene ORGANizer for ADAM20 Gene

Orthologs for ADAM20 Gene

This gene was present in the common ancestor of animals.

Orthologs for ADAM20 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia ADAM20 29 30
  • 99.57 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia LOC519654 29
  • 78.85 (n)
ADAM20 30
  • 70 (a)
OneToOne
Dog
(Canis familiaris)
Mammalia ADAM20 30
  • 69 (a)
OneToOne
Mouse
(Mus musculus)
Mammalia Adam25 29 16
  • 64.34 (n)
Gm5346 30
  • 43 (a)
ManyToMany
Adam20 16
Adam39 16
Platypus
(Ornithorhynchus anatinus)
Mammalia -- 30
  • 38 (a)
ManyToMany
Oppossum
(Monodelphis domestica)
Mammalia -- 30
  • 34 (a)
ManyToMany
Chicken
(Gallus gallus)
Aves -- 30
  • 40 (a)
OneToMany
Lizard
(Anolis carolinensis)
Reptilia -- 30
  • 43 (a)
ManyToMany
-- 30
  • 43 (a)
ManyToMany
-- 30
  • 41 (a)
ManyToMany
-- 30
  • 41 (a)
ManyToMany
-- 30
  • 40 (a)
ManyToMany
-- 30
  • 40 (a)
ManyToMany
Fruit Fly
(Drosophila melanogaster)
Insecta Neu3 30
  • 16 (a)
OneToMany
Worm
(Caenorhabditis elegans)
Secernentea adm-2 30
  • 21 (a)
OneToMany
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 30
  • 32 (a)
ManyToMany
-- 30
  • 32 (a)
ManyToMany
Species where no ortholog for ADAM20 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rat (Rattus norvegicus)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Tropical Clawed Frog (Silurana tropicalis)
  • Wheat (Triticum aestivum)
  • Zebrafish (Danio rerio)

Evolution for ADAM20 Gene

ENSEMBL:
Gene Tree for ADAM20 (if available)
TreeFam:
Gene Tree for ADAM20 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for ADAM20: view image
Alliance of Genome Resources:
Additional Orthologs for ADAM20

Paralogs for ADAM20 Gene

(12) SIMAP similar genes for ADAM20 Gene using alignment to 1 proteins:

  • ADA20_HUMAN
genes like me logo Genes that share paralogs with ADAM20: view

Variants for ADAM20 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for ADAM20 Gene

SNP ID Clinical significance and condition Chr 14 pos Variation AA Info Type
rs1059166 -- p.Phe19Leu

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for ADAM20 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for ADAM20 Gene

Variant ID Type Subtype PubMed ID
esv2763072 CNV gain 21179565
esv3568010 CNV gain 25503493
nsv1050615 CNV gain+loss 25217958
nsv565042 CNV loss 21841781

Variation tolerance for ADAM20 Gene

Residual Variation Intolerance Score: 43.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.21; 39.81% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ADAM20 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
ADAM20
Leiden Open Variation Database (LOVD)
ADAM20

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ADAM20 Gene

Disorders for ADAM20 Gene

Additional Disease Information for ADAM20

No disorders were found for ADAM20 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for ADAM20 Gene

Publications for ADAM20 Gene

  1. ADAM 20 and 21; two novel human testis-specific membrane metalloproteases with similarity to fertilin-alpha. (PMID: 9469942) Hooft van Huijsduijnen R (Gene 1998) 2 3 4
  2. The identification of seven metalloproteinase-disintegrin (ADAM) genes from genomic libraries. (PMID: 10524237) Poindexter K … Cerretti DP (Gene 1999) 3 4
  3. VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. (PMID: 29507755) Yue Y … Liu J (Cell discovery 2018) 3
  4. Sperm-egg fusion disorder in a Chinese male patient was associated with a rare ADAM20 variant. (PMID: 29416755) Sha YW … Liu WW (Oncotarget 2018) 3
  5. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. (PMID: 21873635) Gaudet P … Thomas PD (Briefings in bioinformatics 2011) 3

Products for ADAM20 Gene

Sources for ADAM20 Gene