The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010] See more...

Aliases for ACOT8 Gene

Aliases for ACOT8 Gene

  • Acyl-CoA Thioesterase 8 2 3 4 5
  • HACTE-III 2 3 4
  • PTE-2 2 3 4
  • HTE 2 3 4
  • Nef (Lentivirus Myristoylated Factor) Associated Protein 1 2 3
  • Peroxisomal Acyl-Coenzyme A Thioester Hydrolase 1 3 4
  • Peroxisomal Long-Chain Acyl-CoA Thioesterase 1 3 4
  • Peroxisomal Acyl-CoA Thioesterase 1 2 3
  • Peroxisomal Acyl-CoA Thioesterase 2 3 4
  • Choloyl-Coenzyme A Thioesterase 3 4
  • Acyl-Coenzyme A Thioesterase 8 3 4
  • Choloyl-CoA Hydrolase 2 3
  • Thioesterase II 3 4
  • PTE-1 3 4
  • NAP1 2 3
  • PTE1 3 4
  • HIV-Nef Associated Acyl-CoA Thioesterase 3
  • HIV-Nef-Associated Acyl-CoA Thioesterase 4
  • Long-Chain Fatty-Acyl-CoA Hydrolase 3
  • Peroxisomal Acyl-CoA Thioesterase 2
  • Palmitoyl-CoA Hydrolase 3
  • Thioesterase III 3
  • EC 3.1.2.11 4
  • EC 3.1.2.27 4
  • EC 3.1.2.1 4
  • EC 3.1.2.2 4
  • EC 3.1.2.3 4
  • EC 3.1.2.5 4
  • HACTEIII 4
  • HNAACTE 3
  • ACTEIII 4
  • ACOT8 5
  • PTE2 3

External Ids for ACOT8 Gene

Previous HGNC Symbols for ACOT8 Gene

  • PTE1

Previous GeneCards Identifiers for ACOT8 Gene

  • GC20M043904
  • GC20M044470
  • GC20M041212

Summaries for ACOT8 Gene

Entrez Gene Summary for ACOT8 Gene

  • The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010]

GeneCards Summary for ACOT8 Gene

ACOT8 (Acyl-CoA Thioesterase 8) is a Protein Coding gene. Diseases associated with ACOT8 include Hypertensive Encephalopathy and Epilepsy, Familial Adult Myoclonic, 5. Among its related pathways are Synthesis of bile acids and bile salts and alpha-linolenic (omega3) and linoleic (omega6) acid metabolism. Gene Ontology (GO) annotations related to this gene include signaling receptor binding and palmitoyl-CoA hydrolase activity.

UniProtKB/Swiss-Prot Summary for ACOT8 Gene

  • Acyl-coenzyme A (acyl-CoA) thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:9299485, PubMed:9153233, PubMed:15194431). Acyl-coenzyme A thioesterase 8/ACOT8 display no strong substrate specificity with respect to the carboxylic acid moiety of Acyl-CoAs (By similarity). Hydrolyzes medium length (C2 to C20) straight-chain, saturated and unsaturated acyl-CoAS but is inactive towards substrates with longer aliphatic chains (PubMed:9299485, PubMed:9153233). Moreover, it catalyzes the hydrolysis of CoA esters of bile acids, such as choloyl-CoA and chenodeoxycholoyl-CoA and competes with bile acid CoA:amino acid N-acyltransferase (BAAT) (By similarity). ACOT8 is also able to hydrolyze CoA esters of dicarboxylic acids (By similarity). It is involved in the metabolic regulation of peroxisome proliferation (PubMed:15194431).
  • (Microbial infection) May mediate Nef-induced down-regulation of CD4 cell-surface expression (PubMed:9153233).

Gene Wiki entry for ACOT8 Gene

Additional gene information for ACOT8 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for ACOT8 Gene

Genomics for ACOT8 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for ACOT8 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH20J045855 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE CraniofacialAtlas 600.7 +0.1 130 3 SP1 HNRNPL CREB1 GATAD2A ZBTB33 PRDM10 ZNF629 REST TFE3 RFX1 ZSWIM3 ACOT8 lnc-TNNC2-1 NCOA5 TP53RK WFDC3 ZNF335 SLC35C2 ZSWIM1 PCIF1
GH20J045860 Promoter 0.4 Ensembl 600.7 -2.0 -1996 0.4 CEBPG ACOT8 ZSWIM3 WFDC3 PLTP ZSWIM1
GH20J045933 Promoter/Enhancer 2.3 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 11.9 -78.8 -78843 6.5 SP1 HNRNPL CREB1 GATAD2A CTCF ATF7 PRDM10 REST ZNF629 TFE3 PCIF1 ENSG00000285796 LOC107985388 SNX21 ZNF335 SLC35C2 PLTP NCOA5 ZSWIM3 TP53RK
GH20J045909 Promoter/Enhancer 2.2 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 11.4 -53.6 -53641 3.9 BRCA1 GATAD2A PRDM10 ZNF629 TFE3 RFX1 IKZF1 NFKBIZ KDM1A RCOR2 PLTP NCOA5 ZSWIM3 ZNF335 SLC12A5-AS1 SLC35C2 ZSWIM1 TP53RK PCIF1 ACOT8
GH20J045924 Enhancer 1.1 Ensembl ENCODE dbSUPER 11.1 -67.5 -67501 2.6 GATAD2A NFKBIZ FOXA1 RXRB IRF2 SP1 PBX2 CEBPA FOXK2 TGIF2 WFDC3 PLTP SPATA25 NEURL2 CTSA SNX21 TNNC2 ZSWIM3 ACOT8 UBE2C
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around ACOT8 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for ACOT8

Top Transcription factor binding sites by QIAGEN in the ACOT8 gene promoter:
  • c-Ets-1
  • E47
  • Gfi-1
  • MIF-1
  • MyoD
  • PPAR-alpha
  • Tal-1beta
  • USF-1

Genomic Locations for ACOT8 Gene

Genomic Locations for ACOT8 Gene
chr20:45,841,721-45,857,405
(GRCh38/hg38)
Size:
15,685 bases
Orientation:
Minus strand
chr20:44,470,360-44,486,048
(GRCh37/hg19)
Size:
15,689 bases
Orientation:
Minus strand

Genomic View for ACOT8 Gene

Genes around ACOT8 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ACOT8 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ACOT8 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ACOT8 Gene

Proteins for ACOT8 Gene

  • Protein details for ACOT8 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O14734-ACOT8_HUMAN
    Recommended name:
    Acyl-coenzyme A thioesterase 8
    Protein Accession:
    O14734
    Secondary Accessions:
    • O15261
    • Q17RX4

    Protein attributes for ACOT8 Gene

    Size:
    319 amino acids
    Molecular mass:
    35914 Da
    Quaternary structure:
    • Homodimer (By similarity).
    • (Microbial infection) Interacts with human immunodeficiency virus (HIV-1) Nef (via middle region); this interaction enhances ACOT8 Acyl-CoA thioesterase activity and occurs in a Nef myristoylation-independent manner (PubMed:9299485). According to a second report, the interaction with HIV-1 Nef occurs in a Nef myristoylation-independent manner but does not enhance ACOT8 Acyl-CoA thioesterase activity (PubMed:9153233).

neXtProt entry for ACOT8 Gene

Post-translational modifications for ACOT8 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for ACOT8 Gene

Domains & Families for ACOT8 Gene

Gene Families for ACOT8 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for ACOT8 Gene

InterPro:
Blocks:
  • Acyl-CoA thioesterase
ProtoNet:

Suggested Antigen Peptide Sequences for ACOT8 Gene

GenScript: Design optimal peptide antigens:
  • Thioesterase II (ACOT8_HUMAN)
  • PTE1 protein (Q6FHI2_HUMAN)
  • Acyl-CoA thioesterase 8 (Q9BR14_HUMAN)
  • Acyl-CoA thioesterase 8 (Q9BR15_HUMAN)

Graphical View of Domain Structure for InterPro Entry

O14734

UniProtKB/Swiss-Prot:

ACOT8_HUMAN :
  • Belongs to the C/M/P thioester hydrolase family.
Family:
  • Belongs to the C/M/P thioester hydrolase family.
genes like me logo Genes that share domains with ACOT8: view

Function for ACOT8 Gene

Molecular function for ACOT8 Gene

UniProtKB/Swiss-Prot Function:
Acyl-coenzyme A (acyl-CoA) thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:9299485, PubMed:9153233, PubMed:15194431). Acyl-coenzyme A thioesterase 8/ACOT8 display no strong substrate specificity with respect to the carboxylic acid moiety of Acyl-CoAs (By similarity). Hydrolyzes medium length (C2 to C20) straight-chain, saturated and unsaturated acyl-CoAS but is inactive towards substrates with longer aliphatic chains (PubMed:9299485, PubMed:9153233). Moreover, it catalyzes the hydrolysis of CoA esters of bile acids, such as choloyl-CoA and chenodeoxycholoyl-CoA and competes with bile acid CoA:amino acid N-acyltransferase (BAAT) (By similarity). ACOT8 is also able to hydrolyze CoA esters of dicarboxylic acids (By similarity). It is involved in the metabolic regulation of peroxisome proliferation (PubMed:15194431).
UniProtKB/Swiss-Prot Function:
(Microbial infection) May mediate Nef-induced down-regulation of CD4 cell-surface expression (PubMed:9153233).
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=choloyl-CoA + H2O = cholate + CoA + H(+); Xref=Rhea:RHEA:14541, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29747, ChEBI:CHEBI:57287, ChEBI:CHEBI:57373; EC=3.1.2.27; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=chenodeoxycholoyl-CoA + H2O = chenodeoxycholate + CoA + H(+); Xref=Rhea:RHEA:31511, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:36234, ChEBI:CHEBI:57287, ChEBI:CHEBI:62989; EC=3.1.2.27; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=acetyl-CoA + H2O = acetate + CoA + H(+); Xref=Rhea:RHEA:20289, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=3.1.2.1; Evidence={ECO:0000269|PubMed:10944470, ECO:0000269|PubMed:9299485};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=butanoyl-CoA + H2O = butanoate + CoA + H(+); Xref=Rhea:RHEA:40111, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17968, ChEBI:CHEBI:57287, ChEBI:CHEBI:57371; Evidence={ECO:0000269|PubMed:10944470, ECO:0000269|PubMed:9299485};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=2-methylpropanoyl-CoA + H2O = 2-methylpropanoate + CoA + H(+); Xref=Rhea:RHEA:40799, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:48944, ChEBI:CHEBI:57287, ChEBI:CHEBI:57338; Evidence={ECO:0000269|PubMed:10944470};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=H2O + hexanoyl-CoA = CoA + H(+) + hexanoate; Xref=Rhea:RHEA:40115, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17120, ChEBI:CHEBI:57287, ChEBI:CHEBI:62620; Evidence={ECO:0000269|PubMed:9153233};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=H2O + octanoyl-CoA = CoA + H(+) + octanoate; Xref=Rhea:RHEA:30143, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25646, ChEBI:CHEBI:57287, ChEBI:CHEBI:57386; Evidence={ECO:0000269|PubMed:10944470, ECO:0000269|PubMed:9153233, ECO:0000269|PubMed:9299485};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=decanoyl-CoA + H2O = CoA + decanoate + H(+); Xref=Rhea:RHEA:40059, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:27689, ChEBI:CHEBI:57287, ChEBI:CHEBI:61430; Evidence={ECO:0000269|PubMed:10944470, ECO:0000269|PubMed:9153233, ECO:0000269|PubMed:9299485};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=dodecanoyl-CoA + H2O = CoA + dodecanoate + H(+); Xref=Rhea:RHEA:30135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:18262, ChEBI:CHEBI:57287, ChEBI:CHEBI:57375; Evidence={ECO:0000269|PubMed:9153233};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=H2O + tetradecanoyl-CoA = CoA + H(+) + tetradecanoate; Xref=Rhea:RHEA:40119, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30807, ChEBI:CHEBI:57287, ChEBI:CHEBI:57385; Evidence={ECO:0000269|PubMed:10944470, ECO:0000269|PubMed:9153233, ECO:0000269|PubMed:9299485};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=H2O + hexadecanoyl-CoA = CoA + H(+) + hexadecanoate; Xref=Rhea:RHEA:16645, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379; EC=3.1.2.2; Evidence={ECO:0000269|PubMed:9153233, ECO:0000269|PubMed:9299485};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=H2O + octadecanoyl-CoA = CoA + H(+) + octadecanoate; Xref=Rhea:RHEA:30139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25629, ChEBI:CHEBI:57287, ChEBI:CHEBI:57394; Evidence={ECO:0000269|PubMed:9299485};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=H2O + malonyl-CoA = CoA + H(+) + malonate; Xref=Rhea:RHEA:40219, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15792, ChEBI:CHEBI:57287, ChEBI:CHEBI:57384; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=acetoacetyl-CoA + H2O = acetoacetate + CoA + H(+); Xref=Rhea:RHEA:15673, ChEBI:CHEBI:13705, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57286, ChEBI:CHEBI:57287; EC=3.1.2.11; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=H2O + propanoyl-CoA = CoA + H(+) + propanoate; Xref=Rhea:RHEA:40103, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17272, ChEBI:CHEBI:57287, ChEBI:CHEBI:57392; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=H2O + succinyl-CoA = CoA + H(+) + succinate; Xref=Rhea:RHEA:11516, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30031, ChEBI:CHEBI:57287, ChEBI:CHEBI:57292; EC=3.1.2.3; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=glutaryl-CoA + H2O = CoA + glutarate + H(+); Xref=Rhea:RHEA:40575, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30921, ChEBI:CHEBI:57287, ChEBI:CHEBI:57378; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=H2O + hexanedioyl-CoA = CoA + H(+) + hexanedioate; Xref=Rhea:RHEA:40583, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17128, ChEBI:CHEBI:57287, ChEBI:CHEBI:76327; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=H2O + octanedioyl-CoA = CoA + H(+) + octanedioate; Xref=Rhea:RHEA:40587, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:76282, ChEBI:CHEBI:76317; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=decanedioyl-CoA + H2O = CoA + decanedioate + H(+); Xref=Rhea:RHEA:40591, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:76283, ChEBI:CHEBI:76316; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=dodecanedioyl-CoA + H2O = CoA + dodecanedioate + H(+); Xref=Rhea:RHEA:40595, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:76273, ChEBI:CHEBI:76315; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(9Z)-tetradecenoyl-CoA + H2O = (9Z)-tetradecenoate + CoA + H(+); Xref=Rhea:RHEA:40135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32370, ChEBI:CHEBI:57287, ChEBI:CHEBI:65060; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(9Z)-hexadecenoyl-CoA + H2O = (9Z)-hexadecenoate + CoA + H(+); Xref=Rhea:RHEA:40131, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32372, ChEBI:CHEBI:57287, ChEBI:CHEBI:61540; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(9Z)-octadecenoyl-CoA + H2O = (9Z)-octadecenoate + CoA + H(+); Xref=Rhea:RHEA:40139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(9Z,12Z)-octadecadienoyl-CoA + H2O = (9Z,12Z)-octadecadienoate + CoA + H(+); Xref=Rhea:RHEA:40143, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30245, ChEBI:CHEBI:57287, ChEBI:CHEBI:57383; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=eicosanoyl-CoA + H2O = CoA + eicosanoate + H(+); Xref=Rhea:RHEA:40147, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32360, ChEBI:CHEBI:57287, ChEBI:CHEBI:57380; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA + H(+); Xref=Rhea:RHEA:40151, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32395, ChEBI:CHEBI:57287, ChEBI:CHEBI:57368; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=4,8-dimethylnonanoyl-CoA + H2O = 4,8-dimethylnonanoate + CoA + H(+); Xref=Rhea:RHEA:40223, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:77061, ChEBI:CHEBI:77063; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=2,6-dimethylheptanoyl-CoA + H2O = 2,6-dimethylheptanoate + CoA + H(+); Xref=Rhea:RHEA:59952, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:84847, ChEBI:CHEBI:143533; Evidence={ECO:0000250|UniProtKB:Q8VHK0};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(3S)-hydroxy-3-methylglutaryl-CoA + H2O = 3-hydroxy-3-methylglutarate + CoA + H(+); Xref=Rhea:RHEA:16305, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17325, ChEBI:CHEBI:43074, ChEBI:CHEBI:57287; EC=3.1.2.5; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyl-CoA + H2O = 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + CoA + H(+); Xref=Rhea:RHEA:59936, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:63001, ChEBI:CHEBI:85674; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=2-methyloctadecanoyl-CoA + H2O = 2-methyloctadecanoate + CoA + H(+); Xref=Rhea:RHEA:59940, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:143530, ChEBI:CHEBI:143531; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=H2O + prostaglandin F2alpha-CoA = CoA + H(+) + prostaglandin F2alpha; Xref=Rhea:RHEA:59948, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57404, ChEBI:CHEBI:143532; Evidence={ECO:0000250|UniProtKB:P58137};.
UniProtKB/Swiss-Prot Induction:
Regulated by peroxisome proliferator (such as Clofibrate), via the peroxisome proliferator-activated receptors (PPARs).
UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=10.1 uM for decanoyl-CoA {ECO:0000269|PubMed:9153233}; Vmax=7.1 umol/min/mg enzyme {ECO:0000269|PubMed:9153233};
UniProtKB/Swiss-Prot EnzymeRegulation:
Inhibited by CoASH (IC(50)=10-15 uM). Also inhibited by cysteine-reactive agents.

Enzyme Numbers (IUBMB) for ACOT8 Gene

Phenotypes From GWAS Catalog for ACOT8 Gene

Gene Ontology (GO) - Molecular Function for ACOT8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003986 acetyl-CoA hydrolase activity TAS --
GO:0004778 succinyl-CoA hydrolase activity IEA --
GO:0005515 protein binding IPI 9153233
GO:0016289 CoA hydrolase activity TAS --
GO:0016290 palmitoyl-CoA hydrolase activity IEA,IDA 9299485
genes like me logo Genes that share ontologies with ACOT8: view
genes like me logo Genes that share phenotypes with ACOT8: view

Animal Model Products

  • Taconic Biosciences Mouse Models for ACOT8

CRISPR Products

miRNA for ACOT8 Gene

miRTarBase miRNAs that target ACOT8

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ACOT8

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for ACOT8 Gene

Localization for ACOT8 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ACOT8 Gene

Peroxisome matrix. Note=Predominantly localized in the peroxisome but a localization to the cytosol cannot be excluded. {ECO:0000269 PubMed:10092594, ECO:0000269 PubMed:15194431}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ACOT8 gene
Compartment Confidence
peroxisome 5
cytosol 5
mitochondrion 3
nucleus 3
extracellular 2
plasma membrane 1
cytoskeleton 1
endoplasmic reticulum 1
endosome 1
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Vesicles (3)
  • Mitochondria (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for ACOT8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005777 peroxisome IEA --
GO:0005782 peroxisomal matrix IMP,TAS --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with ACOT8: view

Pathways & Interactions for ACOT8 Gene

genes like me logo Genes that share pathways with ACOT8: view

UniProtKB/Swiss-Prot O14734-ACOT8_HUMAN

  • Pathway: Lipid metabolism; fatty acid metabolism.

Gene Ontology (GO) - Biological Process for ACOT8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006625 protein targeting to peroxisome TAS --
GO:0006629 lipid metabolic process IEA --
GO:0006631 fatty acid metabolic process IEA --
GO:0006637 acyl-CoA metabolic process IEA,TAS --
GO:0006699 bile acid biosynthetic process TAS --
genes like me logo Genes that share ontologies with ACOT8: view

No data available for SIGNOR curated interactions for ACOT8 Gene

Drugs & Compounds for ACOT8 Gene

(15) Drugs for ACOT8 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
chenodeoxycholic acid Approved Pharma Antagonist, Agonist, Full agonist 29
cholic acid Approved Pharma Agonist, Full agonist 0
Gamolenic acid Approved, Investigational Pharma 0
linoleic acid Approved, Experimental Pharma Agonist, Full agonist, Activator, Pore Blocker, Channel blocker 0
oleic acid Approved, Investigational, Vet_approved Pharma Agonist, Full agonist, Activator, Activation 0

(42) Additional Compounds for ACOT8 Gene - From: HMDB and Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
(2E)-Decenoyl-CoA
  • (e)-S-2-Decenoate
  • (e)-S-2-Decenoate CoA
  • (e)-S-2-Decenoate coenzyme A
  • (e)-S-2-Decenoic acid
  • 2-trans-Decenoyl-CoA
10018-95-8
(2E)-Dodecenoyl-CoA
1066-12-2
(2E)-Hexadecenoyl-CoA
  • (e)-C16:1 N-14-CoA
  • (e)-Hexadec-2-enoyl-CoA tetraanion
  • (e)-Hexadec-2-enoyl-coenzyme A tetraanion
  • (e)-Hexadec-2-enoyl-coenzyme A(4-)
  • trans-2-Hexadecenoyl-CoA(4-)
4460-95-1
(2E)-Octenoyl-CoA
  • (e)-S-2-Octenoate
  • (e)-S-2-Octenoate CoA
  • (e)-S-2-Octenoate coenzyme A
  • (e)-S-2-Octenoic acid
  • 2,3-trans-Octenoyl coenzyme A
10018-94-7
(2E)-Tetradecenoyl-CoA
  • (2E)-Tetradecenoyl-coenzyme A
  • trans-Tetra-dec-2-enoyl-CoA
  • trans-Tetra-dec-2-enoyl-coa.
  • trans-Tetra-dec-2-enoyl-coenzyme A
  • trans-Tetra-dec-2-enoyl-coenzyme A.
38795-33-4
genes like me logo Genes that share compounds with ACOT8: view

Transcripts for ACOT8 Gene

mRNA/cDNA for ACOT8 Gene

3 REFSEQ mRNAs :
15 NCBI additional mRNA sequence :
13 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ACOT8

Alternative Splicing Database (ASD) splice patterns (SP) for ACOT8 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b · 2c ^ 3 ^ 4a · 4b · 4c ^ 5a · 5b · 5c · 5d ^ 6a · 6b · 6c · 6d · 6e
SP1: - - - -
SP2: - - - -
SP3: - - -
SP4: - -
SP5: -
SP6: - - -
SP7: - - - - - -
SP8: -
SP9:

Relevant External Links for ACOT8 Gene

GeneLoc Exon Structure for
ACOT8

Expression for ACOT8 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for ACOT8 Gene

Protein differential expression in normal tissues from HIPED for ACOT8 Gene

This gene is overexpressed in Bone (31.0) and Peripheral blood mononuclear cells (7.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for ACOT8 Gene



Protein tissue co-expression partners for ACOT8 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for ACOT8

SOURCE GeneReport for Unigene cluster for ACOT8 Gene:

Hs.444776

mRNA Expression by UniProt/SwissProt for ACOT8 Gene:

O14734-ACOT8_HUMAN
Tissue specificity: Detected in a T-cell line (at protein level). Ubiquitous (PubMed:9153233, PubMed:9299485).

Evidence on tissue expression from TISSUES for ACOT8 Gene

  • Nervous system(4.9)
  • Muscle(4.5)
  • Skin(2.4)
  • Kidney(2.3)
  • Liver(2.3)
  • Lung(2.3)
  • Heart(2.2)
  • Intestine(2.2)
  • Blood(2.1)
genes like me logo Genes that share expression patterns with ACOT8: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for ACOT8 Gene

Orthologs for ACOT8 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for ACOT8 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia ACOT8 30 31
  • 99.79 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia ACOT8 30 31
  • 88.5 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia ACOT8 30 31
  • 87.45 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Acot8 30
  • 85.68 (n)
Mouse
(Mus musculus)
Mammalia Acot8 30 17 31
  • 84.85 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia ACOT8 31
  • 84 (a)
OneToOne
Chicken
(Gallus gallus)
Aves ACOT8 30 31
  • 72.33 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia ACOT8 31
  • 68 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia acot8 30
  • 69.45 (n)
Str.3646 30
African clawed frog
(Xenopus laevis)
Amphibia Xl.13325 30
Zebrafish
(Danio rerio)
Actinopterygii acot8 30 31
  • 67.57 (n)
OneToOne
Worm
(Caenorhabditis elegans)
Secernentea F25E2.3 30 31
  • 48.07 (n)
OneToMany
Y87G2A.2 31
  • 31 (a)
OneToMany
C17C3.3 31
  • 29 (a)
OneToMany
C37H5.13 31
  • 29 (a)
OneToMany
C17C3.1 31
  • 29 (a)
OneToMany
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes TES1 31 33
  • 24 (a)
OneToOne
Thale Cress
(Arabidopsis thaliana)
eudicotyledons AT1G01710 30
  • 50.66 (n)
Species where no ortholog for ACOT8 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Platypus (Ornithorhynchus anatinus)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for ACOT8 Gene

ENSEMBL:
Gene Tree for ACOT8 (if available)
TreeFam:
Gene Tree for ACOT8 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for ACOT8: view image

Paralogs for ACOT8 Gene

(1) SIMAP similar genes for ACOT8 Gene using alignment to 9 proteins:

  • ACOT8_HUMAN
  • E9PIS4_HUMAN
  • E9PJN0_HUMAN
  • E9PMC4_HUMAN
  • E9PRD4_HUMAN
  • F6VBM3_HUMAN
  • H0Y698_HUMAN
  • H7C5A7_HUMAN
  • Q9BR14_HUMAN
genes like me logo Genes that share paralogs with ACOT8: view

No data available for Paralogs for ACOT8 Gene

Variants for ACOT8 Gene

Additional dbSNP identifiers (rs#s) for ACOT8 Gene

Structural Variations from Database of Genomic Variants (DGV) for ACOT8 Gene

Variant ID Type Subtype PubMed ID
esv3645942 CNV loss 21293372
nsv458993 CNV loss 19166990
nsv517296 CNV loss 19592680
nsv586132 CNV loss 21841781
nsv586133 CNV loss 21841781
nsv833990 CNV loss 17160897

Variation tolerance for ACOT8 Gene

Residual Variation Intolerance Score: 91.2% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.71; 57.45% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ACOT8 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
ACOT8

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for ACOT8 Gene

Disorders for ACOT8 Gene

MalaCards: The human disease database

(5) MalaCards diseases for ACOT8 Gene - From: DISEASES

- elite association - COSMIC cancer census association via MalaCards
Search ACOT8 in MalaCards View complete list of genes associated with diseases
genes like me logo Genes that share disorders with ACOT8: view

No data available for UniProtKB/Swiss-Prot and Genatlas for ACOT8 Gene

Publications for ACOT8 Gene

  1. Identification of peroxisomal acyl-CoA thioesterases in yeast and humans. (PMID: 10092594) Jones JM … Gould SJ (The Journal of biological chemistry 1999) 2 3 4 23
  2. Identification of PTE2, a human peroxisomal long-chain acyl-CoA thioesterase. (PMID: 10944470) Jones JM … Gould SJ (Biochemical and biophysical research communications 2000) 3 4 23
  3. Binding of HIV-1 Nef to a novel thioesterase enzyme correlates with Nef-mediated CD4 down-regulation. (PMID: 9153233) Liu LX … Benichou S (The Journal of biological chemistry 1997) 2 3 4
  4. A novel acyl-CoA thioesterase enhances its enzymatic activity by direct binding with HIV Nef. (PMID: 9299485) Watanabe H … Saito T (Biochemical and biophysical research communications 1997) 3 4 23
  5. Analysis of the mouse and human acyl-CoA thioesterase (ACOT) gene clusters shows that convergent, functional evolution results in a reduced number of human peroxisomal ACOTs. (PMID: 16940157) Hunt MC … Alexson SE (FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2006) 2 3

Products for ACOT8 Gene

  • Signalway Proteins for ACOT8

Sources for ACOT8 Gene