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Aliases for MYLK3 Gene

Aliases for MYLK3 Gene

  • Myosin Light Chain Kinase 3 2 3
  • MLCK 3 4 6
  • Cardiac-MyBP-C-Associated Ca/CaM Kinase 3 4
  • Cardiac-MLCK 3 4
  • MLC Kinase 2 3
  • Cardiac-MyBP-C Associated Ca/CaM Kinase 3
  • Putative Myosin Light Chain Kinase 3 3
  • EC 2.7.11.18 4
  • EC 2.7.11 63
  • CaMLCK 3
  • MLCK2 3

External Ids for MYLK3 Gene

Previous GeneCards Identifiers for MYLK3 Gene

  • GC16M045293
  • GC16M032625

Summaries for MYLK3 Gene

Entrez Gene Summary for MYLK3 Gene

  • Phosphorylation of cardiac myosin heavy chains (see MYH7B, MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes (Chan et al., 2008 [PubMed 18202317]).[supplied by OMIM, Jul 2008]

GeneCards Summary for MYLK3 Gene

MYLK3 (Myosin Light Chain Kinase 3) is a Protein Coding gene. Among its related pathways are Regulation of actin cytoskeleton and Immune response CCR3 signaling in eosinophils. GO annotations related to this gene include calmodulin-dependent protein kinase activity and myosin light chain kinase activity. An important paralog of this gene is MYLK4.

UniProtKB/Swiss-Prot for MYLK3 Gene

  • Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility (By similarity).

Tocris Summary for MYLK3 Gene

  • Myosin Light Chain Kinases (MLCKs) are a group of protein serine/threonine kinases that is currently divided into two subtypes, MLCK1 and MLCK2. MLCK1 is found in smooth muscle and phosphorylates myosin II regulatory light chains at Ser19, allowing myosin crossbridges to bind to actin filaments and initiate contraction. In addition to smooth muscle contraction, MLCK1 is involved in a diverse range of biological processes including control of endothelial and vascular permeability, growth initiation of astrocytes processes, neurotransmitter release in the sympathetic nervous system and apoptosis of fibroblasts. MLCK2 is found in striated muscle and co-localizes with phosphorylated MLC filaments to mediate skeletal and cardiac muscle contraction. MLCK3 and MLCK4 have recently been identified but are yet to be fully characterized. MLCKs are activated by Ca2+-calmodulin complexes and require Mg2+ or Ca2+ as a co-factor.

Gene Wiki entry for MYLK3 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MYLK3 Gene

Genomics for MYLK3 Gene

Regulatory Elements for MYLK3 Gene

Genomic Location for MYLK3 Gene

Start:
46,702,282 bp from pter
End:
46,790,407 bp from pter
Size:
88,126 bases
Orientation:
Minus strand

Genomic View for MYLK3 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for MYLK3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MYLK3 Gene

Proteins for MYLK3 Gene

  • Protein details for MYLK3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q32MK0-MYLK3_HUMAN
    Recommended name:
    Myosin light chain kinase 3
    Protein Accession:
    Q32MK0
    Secondary Accessions:
    • B5BUL9
    • B7Z5U8
    • Q32MK1
    • Q96DV1

    Protein attributes for MYLK3 Gene

    Size:
    819 amino acids
    Molecular mass:
    88393 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    No Data Available
    SequenceCaution:
    • Sequence=AAI09098.2; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=CAC42766.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};

    Alternative splice isoforms for MYLK3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for MYLK3 Gene

Proteomics data for MYLK3 Gene at MOPED

Selected DME Specific Peptides for MYLK3 Gene

Post-translational modifications for MYLK3 Gene

Other Protein References for MYLK3 Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains for MYLK3 Gene

Graphical View of Domain Structure for InterPro Entry

Q32MK0

UniProtKB/Swiss-Prot:

MYLK3_HUMAN :
  • Q32MK0
Domain:
  • Contains 1 protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
genes like me logo Genes that share domains with MYLK3: view

Function for MYLK3 Gene

Molecular function for MYLK3 Gene

UniProtKB/Swiss-Prot CatalyticActivity: ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate
UniProtKB/Swiss-Prot Function: Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility (By similarity).

Enzyme Numbers (IUBMB) for MYLK3 Gene

Gene Ontology (GO) - Molecular Function for MYLK3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity --
GO:0004683 calmodulin-dependent protein kinase activity ISS --
GO:0004687 myosin light chain kinase activity ISS --
GO:0004713 protein tyrosine kinase activity --
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with MYLK3: view

Phenotypes for MYLK3 Gene

genes like me logo Genes that share phenotypes with MYLK3: view

Animal Models for MYLK3 Gene

MGI Knock Outs for MYLK3:

Animal Model Products

CRISPR Products

miRNA for MYLK3 Gene

miRTarBase miRNAs that target MYLK3

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for MYLK3

In Situ Assay Products

Flow Cytometry Products

No data available for Transcription Factor Targeting and HOMER Transcription for MYLK3 Gene

Localization for MYLK3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MYLK3 Gene

Cytoplasm.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for MYLK3 Gene COMPARTMENTS Subcellular localization image for MYLK3 gene
Compartment Confidence
cytosol 3
cytoskeleton 2
nucleus 2
extracellular 1
golgi apparatus 1
mitochondrion 1

Gene Ontology (GO) - Cellular Components for MYLK3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm ISS --
GO:0005829 cytosol ISS --
GO:0015629 actin cytoskeleton IEA --
genes like me logo Genes that share ontologies with MYLK3: view

Pathways for MYLK3 Gene

genes like me logo Genes that share pathways with MYLK3: view

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

Gene Ontology (GO) - Biological Process for MYLK3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002528 regulation of vascular permeability involved in acute inflammatory response IMP 18390750
GO:0006468 protein phosphorylation ISS --
GO:0045214 sarcomere organization ISS --
GO:0048769 sarcomerogenesis ISS --
GO:0055003 cardiac myofibril assembly ISS --
genes like me logo Genes that share ontologies with MYLK3: view

Compounds for MYLK3 Gene

(2) Tocris Compounds for MYLK3 Gene

Compound Action Cas Number
ML 7 hydrochloride Selective inhibitor of myosin light chain kinase [110448-33-4]
ML 9 hydrochloride Myosin light chain kinase inhibitor [105637-50-1]

(4) HMDB Compounds for MYLK3 Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
56-65-5
ADP
  • adenosindiphosphorsaeure
58-64-0
Calcium
  • Ca
7440-70-2
Magnesium
  • Magnesium
7439-95-4

(8) Novoseek inferred chemical compound relationships for MYLK3 Gene

Compound -log(P) Hits PubMed IDs
kt 5926 83.4 2
actomyosin 65.1 2
wortmannin 42.5 5
pd 98,059 34.3 2
cytochalasin b 33.5 2
genes like me logo Genes that share compounds with MYLK3: view

Transcripts for MYLK3 Gene

mRNA/cDNA for MYLK3 Gene

(6) Additional mRNA sequences :
(6) REFSEQ mRNAs :
(6) Selected AceView cDNA sequences:
(7) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for MYLK3 Gene

Myosin light chain kinase 3:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for MYLK3

Primer Products

  • OriGene qSTAR qPCR primer pairs in human,mouse,rat for MYLK3

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for MYLK3 Gene

No ASD Table

Relevant External Links for MYLK3 Gene

GeneLoc Exon Structure for
MYLK3
ECgene alternative splicing isoforms for
MYLK3

Expression for MYLK3 Gene

mRNA expression in normal human tissues for MYLK3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for MYLK3 Gene

This gene is overexpressed in Heart - Left Ventricle (23.0) and Heart - Atrial Appendage (19.7).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for MYLK3 Gene

SOURCE GeneReport for Unigene cluster for MYLK3 Gene Hs.130465

mRNA Expression by UniProt/SwissProt for MYLK3 Gene

Q32MK0-MYLK3_HUMAN
Tissue specificity: Restricted to heart.
genes like me logo Genes that share expressions with MYLK3: view

In Situ Assay Products

Orthologs for MYLK3 Gene

This gene was present in the common ancestor of animals.

Orthologs for MYLK3 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia MYLK3 35
  • 99.43 (n)
  • 99.27 (a)
MYLK3 36
  • 99 (a)
OneToOne
cow
(Bos Taurus)
Mammalia MYLK3 35
  • 82.1 (n)
  • 76.21 (a)
MYLK3 36
  • 76 (a)
OneToOne
dog
(Canis familiaris)
Mammalia MYLK3 35
  • 77.85 (n)
  • 72.04 (a)
MYLK3 36
  • 66 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Mylk3 35
  • 78.02 (n)
  • 72.17 (a)
Mylk3 16
Mylk3 36
  • 70 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia MYLK3 36
  • 58 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia MYLK3 36
  • 64 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Mylk3 35
  • 77.94 (n)
  • 71.09 (a)
chicken
(Gallus gallus)
Aves MYLK3 35
  • 57.68 (n)
  • 53.55 (a)
MYLK3 36
  • 48 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia MYLK3 36
  • 68 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia mylk3 35
  • 63.8 (n)
  • 60.88 (a)
zebrafish
(Danio rerio)
Actinopterygii mylk3 36
  • 41 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta sqa 36
  • 17 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea ZC373.4 36
  • 13 (a)
OneToMany
Species with no ortholog for MYLK3:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for MYLK3 Gene

ENSEMBL:
Gene Tree for MYLK3 (if available)
TreeFam:
Gene Tree for MYLK3 (if available)

Paralogs for MYLK3 Gene

Paralogs for MYLK3 Gene

genes like me logo Genes that share paralogs with MYLK3: view

Variants for MYLK3 Gene

Sequence variations from dbSNP and Humsavar for MYLK3 Gene

SNP ID Clin Chr 16 pos Sequence Context AA Info Type MAF
rs28174 -- 46,731,234(-) TGCCC(C/T)GGGGC intron-variant
rs28184 -- 46,739,549(-) ggaag(A/G)tgagg intron-variant
rs28185 -- 46,739,840(-) gaaca(A/C)ctcgg intron-variant
rs36459 -- 46,730,383(-) GAGCC(C/T)ACGTG intron-variant
rs36460 -- 46,730,570(-) TTGGT(G/T)GGGGG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for MYLK3 Gene

Variant ID Type Subtype PubMed ID
nsv526656 CNV Gain 19592680

Relevant External Links for MYLK3 Gene

HapMap Linkage Disequilibrium report
MYLK3

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MYLK3 Gene

Disorders for MYLK3 Gene

(3) Novoseek inferred disease relationships for MYLK3 Gene

Disease -log(P) Hits PubMed IDs
bowel disease 60.4 1
necrosis 17.9 2
tumors 0 5
genes like me logo Genes that share disorders with MYLK3: view

No data available for UniProtKB/Swiss-Prot for MYLK3 Gene

Publications for MYLK3 Gene

  1. A cardiac myosin light chain kinase regulates sarcomere assembly in the vertebrate heart. (PMID: 17885681) Seguchi O. … Kitakaze M. (J. Clin. Invest. 2007) 2 3 4
  2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4
  3. Mechanism of IL-1beta-induced increase in intestinal epithelial tight junction permeability. (PMID: 18390750) Al-Sadi R. … Ma T.Y. (J. Immunol. 2008) 3 23
  4. Presynaptic release probability and readily releasable pool size are regulated by two independent mechanisms during posttetanic potentiation at the calyx of Held synapse. (PMID: 18685020) Lee J.S. … Lee S.H. (J. Neurosci. 2008) 23
  5. Dual role for myosin II in GLUT4-mediated glucose uptake in 3T3-L1 adipocytes. (PMID: 18773891) Fulcher F.K. … Patel Y.M. (Exp. Cell Res. 2008) 23

Products for MYLK3 Gene

  • Addgene plasmids for MYLK3

Sources for MYLK3 Gene

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