Free for academic non-profit institutions. Other users need a Commercial license

Aliases for UBE2M Gene

Aliases for UBE2M Gene

  • Ubiquitin Conjugating Enzyme E2 M 2 3 5
  • Ubiquitin-Conjugating Enzyme E2M (UBC12 Homolog, Yeast) 2 3
  • NEDD8 Carrier Protein 3 4
  • NEDD8 Protein Ligase 3 4
  • UBC12 3 4
  • Ubiquitin-Conjugating Enzyme E2M (Homologous To Yeast UBC12) 2
  • Ubiquitin-Conjugating Enzyme E2 M 4
  • Ubiquitin-Conjugating Enzyme E2M 2
  • Ubiquitin Conjugating Enzyme E2M 3
  • NEDD8-Conjugating Enzyme Ubc12 3
  • Ubiquitin Carrier Protein M 3
  • Ubiquitin-Protein Ligase M 3
  • Yeast UBC12 Homolog 3
  • EC 6.3.2.- 4
  • EC 6.3.2 61
  • UBC-RS2 3
  • HUbc12 3

External Ids for UBE2M Gene

Previous GeneCards Identifiers for UBE2M Gene

  • GC19M059965
  • GC19M059521
  • GC19M063742
  • GC19M063758
  • GC19M059067

Summaries for UBE2M Gene

Entrez Gene Summary for UBE2M Gene

  • The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008]

GeneCards Summary for UBE2M Gene

UBE2M (Ubiquitin Conjugating Enzyme E2 M) is a Protein Coding gene. Diseases associated with UBE2M include Familial Hypertension. Among its related pathways are Transcriptional activity of SMAD2/SMAD3-SMAD4 heterotrimer and CLEC7A (Dectin-1) signaling. GO annotations related to this gene include ubiquitin-protein transferase activity and ubiquitin protein ligase activity. An important paralog of this gene is UBE2F.

UniProtKB/Swiss-Prot for UBE2M Gene

  • Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.

Gene Wiki entry for UBE2M Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for UBE2M Gene

Genomics for UBE2M Gene

Regulatory Elements for UBE2M Gene

Enhancers for UBE2M Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH19G058517 2.1 FANTOM5 Ensembl ENCODE dbSUPER 13.1 +39.3 39268 4.9 MLX CREB3L1 AGO1 ZFP64 FEZF1 DMAP1 YY1 SLC30A9 ZNF416 ZNF143 ZNF324B ZNF549 CENPBD1P1 MZF1 ZNF134 ZNF606 LOC100128398 ZNF324 ZBTB45 ZNF418
GH19G058580 1.2 Ensembl ENCODE 12.3 -22.3 -22289 1.5 PKNOX1 ATF1 CREB3L1 GTF3C2 ZNF766 ZNF143 KDM4B REST ZNF592 MEF2D TRIM28 ZNF587B ZNF324B ZNF134 ZNF587 CENPBD1P1 ZBTB45 UBE2M CHMP2A ZNF417
GH19G058522 0.4 dbSUPER 13.1 +36.1 36056 0.6 ZNF7 TRIM28 CHMP2A UBE2M CENPBD1P1 MZF1 RNU6-1337P ZNF446 ZNF324 ZNF324B PIR40518
GH19G058537 1.1 FANTOM5 ENCODE 1.6 +20.7 20657 2.3 SOX13 RBBP5 HBP1 ETS1 POLR2A NR2F6 SOX5 RNF2 SP5 BHLHE40 ZNF584 ZSCAN18 LOC100506634 ZNF8 ZNF544 TRIM28 ZNF329 ZNF497 ZNF324 ZNF135
GH19G058553 1.4 ENCODE dbSUPER 0.7 +1.9 1864 7.2 CREB3L1 AGO1 FEZF1 DMAP1 YY1 SLC30A9 ZNF143 SP3 NFYC ZFP41 ZNF606 ZNF417 TRIM28 MZF1-AS1 ZNF550 ZNF134 ZNF584 CENPBD1P1 ZNF776 ZNF551
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around UBE2M on UCSC Golden Path with GeneCards custom track

Promoters for UBE2M Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000289365 276 2201 CREB3L1 AGO1 FEZF1 DMAP1 YY1 SLC30A9 SP3 NFYC ZFP41 MEF2D

Transcription factor binding sites by QIAGEN in the UBE2M gene promoter:

Genomic Location for UBE2M Gene

Chromosome:
19
Start:
58,555,712 bp from pter
End:
58,558,976 bp from pter
Size:
3,265 bases
Orientation:
Minus strand

Genomic View for UBE2M Gene

Genes around UBE2M on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
UBE2M Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for UBE2M Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for UBE2M Gene

Proteins for UBE2M Gene

  • Protein details for UBE2M Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P61081-UBC12_HUMAN
    Recommended name:
    NEDD8-conjugating enzyme Ubc12
    Protein Accession:
    P61081
    Secondary Accessions:
    • O76069
    • Q8VC50

    Protein attributes for UBE2M Gene

    Size:
    183 amino acids
    Molecular mass:
    20900 Da
    Quaternary structure:
    • Interacts with UBA3, DCUN1D1 and RBX1.

    Three dimensional structures from OCA and Proteopedia for UBE2M Gene

neXtProt entry for UBE2M Gene

Selected DME Specific Peptides for UBE2M Gene

P61081:
  • ELNLPKTC
  • DDLLNFKLVICPDEGFYK
  • KKASAAQLRIQKDINELNLP
  • CLNILREDW

Post-translational modifications for UBE2M Gene

  • The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.
  • Ubiquitination at Lys3, Lys8, isoforms=36, Lys75, and isoforms=81
  • Modification sites at PhosphoSitePlus

Other Protein References for UBE2M Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for UBE2M (UBE2M)

Domains & Families for UBE2M Gene

Gene Families for UBE2M Gene

Suggested Antigen Peptide Sequences for UBE2M Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P61081

UniProtKB/Swiss-Prot:

UBC12_HUMAN :
  • Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
  • Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
Domain:
  • Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
Family:
  • Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
genes like me logo Genes that share domains with UBE2M: view

Function for UBE2M Gene

Molecular function for UBE2M Gene

UniProtKB/Swiss-Prot CatalyticActivity:
ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.
UniProtKB/Swiss-Prot Function:
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.

Enzyme Numbers (IUBMB) for UBE2M Gene

Gene Ontology (GO) - Molecular Function for UBE2M Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004842 ubiquitin-protein transferase activity TAS 9694792
GO:0005515 protein binding IPI 15361859
GO:0005524 ATP binding IEA --
GO:0016874 ligase activity IEA --
GO:0018169 ribosomal S6-glutamic acid ligase activity IDA 15361859
genes like me logo Genes that share ontologies with UBE2M: view
genes like me logo Genes that share phenotypes with UBE2M: view

Animal Models for UBE2M Gene

MGI Knock Outs for UBE2M:

Animal Model Products

  • Taconic Biosciences Mouse Models for UBE2M

CRISPR Products

miRNA for UBE2M Gene

miRTarBase miRNAs that target UBE2M

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for UBE2M

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for UBE2M Gene

Localization for UBE2M Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for UBE2M gene
Compartment Confidence
extracellular 5
cytosol 5
nucleus 3

Gene Ontology (GO) - Cellular Components for UBE2M Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005654 nucleoplasm TAS --
GO:0005737 cytoplasm IBA --
GO:0005829 cytosol TAS --
GO:0070062 extracellular exosome IDA 23376485
genes like me logo Genes that share ontologies with UBE2M: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for UBE2M Gene

Pathways & Interactions for UBE2M Gene

genes like me logo Genes that share pathways with UBE2M: view

UniProtKB/Swiss-Prot P61081-UBC12_HUMAN

  • Pathway: Protein modification; protein neddylation.

Gene Ontology (GO) - Biological Process for UBE2M Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006464 cellular protein modification process TAS 9694792
GO:0007179 transforming growth factor beta receptor signaling pathway TAS --
GO:0016567 protein ubiquitination IEA --
GO:0038061 NIK/NF-kappaB signaling TAS --
GO:0043525 positive regulation of neuron apoptotic process IEA --
genes like me logo Genes that share ontologies with UBE2M: view

No data available for SIGNOR curated interactions for UBE2M Gene

Drugs & Compounds for UBE2M Gene

(3) Drugs for UBE2M Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Phosphoric acid Approved Pharma 0
Adenosine monophosphate Approved Nutra 0
Adenosine triphosphate Approved Nutra 0

(1) Additional Compounds for UBE2M Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
pyrophosphate
  • (4-)Diphosphoric acid ion
  • (P2O74-)Diphosphate
  • Diphosphate
  • Diphosphoric acid
  • PPi
14000-31-8
genes like me logo Genes that share compounds with UBE2M: view

Transcripts for UBE2M Gene

Unigene Clusters for UBE2M Gene

Ubiquitin-conjugating enzyme E2M:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for UBE2M

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for UBE2M Gene

ExUns: 1 ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b
SP1: -
SP2: -
SP3:
SP4:

Relevant External Links for UBE2M Gene

GeneLoc Exon Structure for
UBE2M
ECgene alternative splicing isoforms for
UBE2M

Expression for UBE2M Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for UBE2M Gene

Protein differential expression in normal tissues from HIPED for UBE2M Gene

This gene is overexpressed in Peripheral blood mononuclear cells (7.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for UBE2M Gene



Protein tissue co-expression partners for UBE2M Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of UBE2M Gene:

UBE2M

SOURCE GeneReport for Unigene cluster for UBE2M Gene:

Hs.406068

Evidence on tissue expression from TISSUES for UBE2M Gene

  • Skin(4.3)
  • Nervous system(2.8)
  • Muscle(2.3)
  • Intestine(2.1)
  • Heart(2)
genes like me logo Genes that share expression patterns with UBE2M: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for UBE2M Gene

Orthologs for UBE2M Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for UBE2M Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia UBE2M 34 35
  • 99.77 (n)
dog
(Canis familiaris)
Mammalia UBE2M 34 35
  • 97.27 (n)
cow
(Bos Taurus)
Mammalia UBE2M 34 35
  • 96.54 (n)
rat
(Rattus norvegicus)
Mammalia Ube2m 34
  • 94.9 (n)
mouse
(Mus musculus)
Mammalia Ube2m 34 16 35
  • 94.35 (n)
chicken
(Gallus gallus)
Aves UBE2M 35
  • 47 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia UBE2M 35
  • 98 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia ube2m 34
  • 81.42 (n)
MGC75750 34
zebrafish
(Danio rerio)
Actinopterygii wu:fb58e05 34
  • 81.24 (n)
wufb58e05 34
fruit fly
(Drosophila melanogaster)
Insecta CG7375 36
  • 75 (a)
Ubc12 34 35
  • 73.56 (n)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP005916 34
  • 70.13 (n)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ADL035C 34
  • 55.49 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes UBC12 34 35 37
  • 50.93 (n)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0C17248g 34
  • 47.25 (n)
thale cress
(Arabidopsis thaliana)
eudicotyledons AT2G18600 34
  • 59.89 (n)
soybean
(Glycine max)
eudicotyledons Gma.17973 34
rice
(Oryza sativa)
Liliopsida Os08g0374100 34
  • 59.52 (n)
Os.14656 34
barley
(Hordeum vulgare)
Liliopsida Hv.3803 34
wheat
(Triticum aestivum)
Liliopsida Ta.3716 34
corn
(Zea mays)
Liliopsida Zm.4335 34
bread mold
(Neurospora crassa)
Ascomycetes NCU03610 34
  • 62.3 (n)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.4912 35
  • 62 (a)
OneToOne
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes ubc12 34
  • 54.17 (n)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.1924 34
Species where no ortholog for UBE2M was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • worm (Caenorhabditis elegans)

Evolution for UBE2M Gene

ENSEMBL:
Gene Tree for UBE2M (if available)
TreeFam:
Gene Tree for UBE2M (if available)

Paralogs for UBE2M Gene

Paralogs for UBE2M Gene

Pseudogenes.org Pseudogenes for UBE2M Gene

genes like me logo Genes that share paralogs with UBE2M: view

Variants for UBE2M Gene

Sequence variations from dbSNP and Humsavar for UBE2M Gene

SNP ID Clin Chr 19 pos Sequence Context AA Info Type
rs1000133837 -- 58,555,279(+) CAAGC(A/C)AGTAC downstream-variant-500B, upstream-variant-2KB, utr-variant-5-prime
rs1001524540 -- 58,559,789(+) CTGCA(A/G)ATGCT intron-variant, upstream-variant-2KB
rs1002285561 -- 58,555,552(+) AGTCA(A/T)GGTAT downstream-variant-500B, upstream-variant-2KB, utr-variant-5-prime
rs1002316712 -- 58,555,386(+) AAGGC(C/T)CCTCC downstream-variant-500B, upstream-variant-2KB, utr-variant-5-prime
rs1002934396 -- 58,558,783(+) CTCCG(C/T)GAGGG upstream-variant-2KB, utr-variant-5-prime

Structural Variations from Database of Genomic Variants (DGV) for UBE2M Gene

Variant ID Type Subtype PubMed ID
dgv3687n100 CNV gain 25217958
dgv3688n100 CNV gain 25217958
nsv1057077 CNV gain 25217958
nsv1057206 CNV gain 25217958
nsv1160670 CNV deletion 26073780
nsv515731 CNV gain+loss 19592680
nsv828657 CNV gain 20364138
nsv952156 CNV deletion 24416366

Variation tolerance for UBE2M Gene

Residual Variation Intolerance Score: 49.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.22; 5.00% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for UBE2M Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
UBE2M

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for UBE2M Gene

Disorders for UBE2M Gene

MalaCards: The human disease database

(1) MalaCards diseases for UBE2M Gene - From: GeneCards

Disorder Aliases PubMed IDs
familial hypertension
  • genetic hypertension
- elite association - COSMIC cancer census association via MalaCards
Search UBE2M in MalaCards View complete list of genes associated with diseases

Relevant External Links for UBE2M

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
UBE2M
genes like me logo Genes that share disorders with UBE2M: view

No data available for UniProtKB/Swiss-Prot and Genatlas for UBE2M Gene

Publications for UBE2M Gene

  1. A dominant-negative UBC12 mutant sequesters NEDD8 and inhibits NEDD8 conjugation in vivo. (PMID: 10828074) Wada H. … Kamitani T. (J. Biol. Chem. 2000) 3 4 22 64
  2. A new NEDD8-ligating system for cullin-4A. (PMID: 9694792) Osaka F. … Kato S. (Genes Dev. 1998) 2 3 4 64
  3. Structural conservation of distinctive N-terminal acetylation-dependent interactions across a family of mammalian NEDD8 ligation enzymes. (PMID: 23201271) Monda J.K. … Schulman B.A. (Structure 2013) 3 4 64
  4. N-terminal acetylation acts as an avidity enhancer within an interconnected multiprotein complex. (PMID: 21940857) Scott D.C. … Schulman B.A. (Science 2011) 3 4 64
  5. E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. (PMID: 19250909) Huang D.T. … Schulman B.A. (Mol. Cell 2009) 3 4 64

Products for UBE2M Gene

Sources for UBE2M Gene

Content
Loading form....