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Aliases for SPHK1 Gene

Aliases for SPHK1 Gene

  • Sphingosine Kinase 1 2 3
  • SPK 1 3 4
  • SK 1 3 4
  • SPHK 3 4
  • EC 2.7.1.91 4
  • SPK 4

External Ids for SPHK1 Gene

Previous GeneCards Identifiers for SPHK1 Gene

  • GC17U990140
  • GC17P077371
  • GC17P074843
  • GC17P074977
  • GC17P071890
  • GC17P074380
  • GC17P069809

Summaries for SPHK1 Gene

Entrez Gene Summary for SPHK1 Gene

  • The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

GeneCards Summary for SPHK1 Gene

SPHK1 (Sphingosine Kinase 1) is a Protein Coding gene. Diseases associated with SPHK1 include renal fibrosis. Among its related pathways are Signaling by GPCR and Interleukin receptor SHC signaling. GO annotations related to this gene include magnesium ion binding and protein phosphatase 2A binding. An important paralog of this gene is SPHK2.

UniProtKB/Swiss-Prot for SPHK1 Gene

  • Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.

Tocris Summary for SPHK1 Gene

  • Sphingosine kinase (SphK) mediates the conversion of sphingosine to sphingosine-1-phosphate, a key sphingolipid signaling molecule involved in cell growth, survival, differentiation and motility. Two mammalian isoforms have been cloned - SPHK1 and SPHK2.

Gene Wiki entry for SPHK1 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for SPHK1 Gene

Genomics for SPHK1 Gene

Regulatory Elements for SPHK1 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for SPHK1 Gene

Chromosome:
17
Start:
76,376,584 bp from pter
End:
76,387,860 bp from pter
Size:
11,277 bases
Orientation:
Plus strand

Genomic View for SPHK1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for SPHK1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for SPHK1 Gene

Proteins for SPHK1 Gene

  • Protein details for SPHK1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9NYA1-SPHK1_HUMAN
    Recommended name:
    Sphingosine kinase 1
    Protein Accession:
    Q9NYA1
    Secondary Accessions:
    • Q8N632
    • Q96GK1
    • Q9HD92
    • Q9NY70
    • Q9NYL3

    Protein attributes for SPHK1 Gene

    Size:
    384 amino acids
    Molecular mass:
    42518 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Interacts with ACY1 (By similarity). Binds to calmodulin. Interacts with SPHKAP. Interacts with CIB1, the interaction occurs in a calcium-dependent manner.
    Miscellaneous:
    • Sphingosine 1-phosphate stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling

    Three dimensional structures from OCA and Proteopedia for SPHK1 Gene

    Alternative splice isoforms for SPHK1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for SPHK1 Gene

Proteomics data for SPHK1 Gene at MOPED

Post-translational modifications for SPHK1 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Antibody Products

  • R&D Systems Antibodies for SPHK1 (Sphingosine Kinase 1/SPHK1)
  • Cell Signaling Technology (CST) Antibodies for SPHK1 (SPHK1)

Protein Products

No data available for DME Specific Peptides for SPHK1 Gene

Domains & Families for SPHK1 Gene

Gene Families for SPHK1 Gene

Protein Domains for SPHK1 Gene

Suggested Antigen Peptide Sequences for SPHK1 Gene

Graphical View of Domain Structure for InterPro Entry

Q9NYA1

UniProtKB/Swiss-Prot:

SPHK1_HUMAN :
  • Contains 1 DAGKc domain.
Domain:
  • Contains 1 DAGKc domain.
genes like me logo Genes that share domains with SPHK1: view

Function for SPHK1 Gene

Molecular function for SPHK1 Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=14 uM for sphingosine {ECO:0000269 PubMed:10947957}; KM=20 uM for dihydrosphingosine {ECO:0000269 PubMed:10947957}; KM=77 uM for ATP {ECO:0000269 PubMed:10947957}; pH dependence: Optimum pH is 7.4. {ECO:0000269 PubMed:10947957};
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + sphinganine = ADP + sphinganine 1-phosphate
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + sphingosine = ADP + sphingosine 1-phosphate
UniProtKB/Swiss-Prot Function:
Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.

Enzyme Numbers (IUBMB) for SPHK1 Gene

Gene Ontology (GO) - Molecular Function for SPHK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000287 magnesium ion binding ISS --
GO:0003677 DNA binding IDA 12393916
GO:0005515 protein binding IPI 12080051
GO:0005516 calmodulin binding IDA 10947957
GO:0005524 ATP binding IDA 12393916
genes like me logo Genes that share ontologies with SPHK1: view
genes like me logo Genes that share phenotypes with SPHK1: view

Animal Models for SPHK1 Gene

MGI Knock Outs for SPHK1:

Animal Model Products

miRNA for SPHK1 Gene

miRTarBase miRNAs that target SPHK1

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for SPHK1

In Situ Assay Products

Flow Cytometry Products

No data available for Transcription Factor Targets and HOMER Transcription for SPHK1 Gene

Localization for SPHK1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for SPHK1 Gene

Cytoplasm. Nucleus. Cell membrane. Note=Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for SPHK1 Gene COMPARTMENTS Subcellular localization image for SPHK1 gene
Compartment Confidence
cytosol 5
nucleus 5
plasma membrane 5
extracellular 2
cytoskeleton 1
endoplasmic reticulum 1
lysosome 1
mitochondrion 1
vacuole 1

Gene Ontology (GO) - Cellular Components for SPHK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005737 cytoplasm IDA 15623571
GO:0005829 cytosol TAS 10863092
GO:0005886 plasma membrane IDA 19854831
genes like me logo Genes that share ontologies with SPHK1: view

Pathways & Interactions for SPHK1 Gene

genes like me logo Genes that share pathways with SPHK1: view

PCR Array Products

SIGNOR curated interactions for SPHK1 Gene

Is activated by:

Gene Ontology (GO) - Biological Process for SPHK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001568 blood vessel development IEA --
GO:0003376 sphingosine-1-phosphate signaling pathway IMP 23106337
GO:0006457 protein folding TAS --
GO:0006665 sphingolipid metabolic process TAS --
GO:0006670 sphingosine metabolic process IDA 20577214
genes like me logo Genes that share ontologies with SPHK1: view

Drugs & Compounds for SPHK1 Gene

(24) Drugs for SPHK1 Gene - From: NovoSeek, HMDB, DGIdb, ApexBio, and DrugBank

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Fingolimod Approved, Investigational Pharma Enzyme, substrate 82
Adenosine triphosphate Approved Nutra 0
Doxorubicin Approved, Investigational Pharma Topo II inhibitor,immunosuppresive antineoplastic antibiotic 1525
Phosphatidylserine Approved Nutra 0
Sphingosine Experimental Pharma 0

(42) Additional Compounds for SPHK1 Gene - From: HMDB, NovoSeek, and Tocris

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
N,N-Dimethylsphingosine
  • (S-(R,S-(E)))-2-(dimethylamino)-4-Octadecene-1,3-diol
  • DMS
  • N,N-Dimethyl-D-erythro-sphingosine
  • N,N-Dimethyl-D-erythro-sphingosine]
  • N,N-Dimethyl-erythro-sphingosine
122314-67-4, 119567-63-4
sphinganine 1-phosphate
  • 2-Amino-3-hydroxyoctadecyl dihydrogen phosphate
  • Dihydrosphingosine 1-phosphate
  • Dihydrosphingosine-1-phosphate
  • Sphinganine 1-phosphate
19794-97-9
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Antagonist, Gating inhibitor 58-64-0
Cer(d18:0/12:0)
Cer(d18:0/14:0)
genes like me logo Genes that share compounds with SPHK1: view

Transcripts for SPHK1 Gene

Unigene Clusters for SPHK1 Gene

Sphingosine kinase 1:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for SPHK1

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for SPHK1 Gene

ExUns: 1 ^ 2 ^ 3a · 3b ^ 4 ^ 5a · 5b · 5c · 5d ^ 6a · 6b · 6c ^ 7a · 7b · 7c · 7d ^ 8 ^ 9a · 9b · 9c · 9d ^ 10a · 10b
SP1: - - - - - - - - - - - -
SP2: - - - - -
SP3: - - - -
SP4: - - - -
SP5: - - - - - - - - - -
SP6: - - - - - - -
SP7: - - - - - - -
SP8: - - -
SP9: - - - - - - - -
SP10: - -
SP11:

Relevant External Links for SPHK1 Gene

GeneLoc Exon Structure for
SPHK1
ECgene alternative splicing isoforms for
SPHK1

Expression for SPHK1 Gene

mRNA expression in normal human tissues for SPHK1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for SPHK1 Gene

This gene is overexpressed in Bone (29.8), Plasma (17.8), Urinary Bladder (7.2), Testis (7.1), and Retina (6.9).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for SPHK1 Gene



SOURCE GeneReport for Unigene cluster for SPHK1 Gene Hs.68061

mRNA Expression by UniProt/SwissProt for SPHK1 Gene

Q9NYA1-SPHK1_HUMAN
Tissue specificity: Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.
genes like me logo Genes that share expression patterns with SPHK1: view

Primer Products

In Situ Assay Products

No data available for mRNA differential expression in normal tissues and Protein tissue co-expression partners for SPHK1 Gene

Orthologs for SPHK1 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for SPHK1 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia SPHK1 35
  • 82.53 (n)
  • 80.57 (a)
SPHK1 36
  • 79 (a)
OneToOne
dog
(Canis familiaris)
Mammalia SPHK1 35
  • 86.01 (n)
  • 88.92 (a)
SPHK1 36
  • 89 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Sphk1 35
  • 80.34 (n)
  • 82.61 (a)
Sphk1 16
Sphk1 36
  • 79 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia SPHK1 35
  • 99.3 (n)
  • 99.74 (a)
SPHK1 36
  • 99 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Sphk1 35
  • 78.66 (n)
  • 77.29 (a)
oppossum
(Monodelphis domestica)
Mammalia SPHK1 36
  • 74 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 90 (a)
OneToMany
-- 36
  • 66 (a)
OneToMany
chicken
(Gallus gallus)
Aves SPHK1 35
  • 65.35 (n)
  • 58.31 (a)
SPHK1 36
  • 54 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia SPHK1 36
  • 40 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia sphk1 35
  • 59.65 (n)
  • 55.4 (a)
zebrafish
(Danio rerio)
Actinopterygii LOC100331840 35
  • 61.38 (n)
  • 55.39 (a)
SPHK1 36
  • 54 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta Sk1 37
  • 42 (a)
Sk2 35
  • 51.63 (n)
  • 39.78 (a)
Sk1 36
  • 22 (a)
ManyToMany
Sk2 36
  • 21 (a)
ManyToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP006995 35
  • 52.87 (n)
  • 40.48 (a)
worm
(Caenorhabditis elegans)
Secernentea C34C6.5 37
  • 28 (a)
sphk-1 36
  • 23 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes LCB4 36
  • 16 (a)
ManyToMany
LCB5 36
  • 15 (a)
ManyToMany
LCB5 38
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 29 (a)
OneToMany
Species with no ortholog for SPHK1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for SPHK1 Gene

ENSEMBL:
Gene Tree for SPHK1 (if available)
TreeFam:
Gene Tree for SPHK1 (if available)

Paralogs for SPHK1 Gene

Paralogs for SPHK1 Gene

(1) SIMAP similar genes for SPHK1 Gene using alignment to 5 proteins:

genes like me logo Genes that share paralogs with SPHK1: view

Variants for SPHK1 Gene

Sequence variations from dbSNP and Humsavar for SPHK1 Gene

SNP ID Clin Chr 17 pos Sequence Context AA Info Type MAF
rs191520 -- 76,388,326(-) aactt(C/T)tgggc downstream-variant-500B
rs346797 -- 76,382,820(+) CCAGT(A/C)CCCTC upstream-variant-2KB, intron-variant
rs346798 -- 76,382,161(+) CCCCC(A/C/G)ACCCG upstream-variant-2KB, intron-variant
rs346799 -- 76,388,042(-) AGAGG(C/T)CCCCC downstream-variant-500B
rs346800 -- 76,387,991(-) CAGAA(C/T)GACTA downstream-variant-500B

Structural Variations from Database of Genomic Variants (DGV) for SPHK1 Gene

Variant ID Type Subtype PubMed ID
nsv833547 CNV Loss 17160897
nsv2145 CNV Loss 18451855
nsv833549 CNV Gain 17160897
esv23649 CNV Loss 19812545

Variation tolerance for SPHK1 Gene

Residual Variation Intolerance Score: 25.23% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 8.46; 85.74% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for SPHK1 Gene

HapMap Linkage Disequilibrium report
SPHK1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for SPHK1 Gene

Disorders for SPHK1 Gene

MalaCards: The human disease database

(1) MalaCards diseases for SPHK1 Gene - From: DISEASES, NovoSeek, and GeneCards

Disorder Aliases PubMed IDs
renal fibrosis
- elite association
Search SPHK1 in MalaCards View complete list of genes associated with diseases

Relevant External Links for SPHK1

Human Genome Epidemiology (HuGE) Navigator
SPHK1
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
SPHK1
genes like me logo Genes that share disorders with SPHK1: view

No data available for UniProtKB/Swiss-Prot and Genatlas for SPHK1 Gene

Publications for SPHK1 Gene

  1. Expression and regulation of enzymes in the ceramide metabolic pathway in human retinal pigment epithelial cells and their relevance to retinal degeneration. (PMID: 19765607) Zhu D. … Kannan R. (Vision Res. 2010) 23 67
  2. Translocation of sphingosine kinase 1 to the plasma membrane is mediated by calcium- and integrin-binding protein 1. (PMID: 19854831) Jarman K.E. … Pitson S.M. (J. Biol. Chem. 2010) 23 67
  3. CXCL4-induced monocyte survival, cytokine expression, and oxygen radical formation is regulated by sphingosine kinase 1. (PMID: 20104488) Kasper B. … Petersen F. (Eur. J. Immunol. 2010) 23 67
  4. An active form of sphingosine kinase-1 is released in the extracellular medium as component of membrane vesicles shed by two human tumor cell lines. (PMID: 20508814) Rigogliuso S. … Vittorelli M.L. (J Oncol 2010) 23 67
  5. Sphingosine kinase 1 regulates mucin production via ERK phosphorylation. (PMID: 19835973) Kono Y. … Nishimura Y. (Pulm Pharmacol Ther 2010) 23 67

Products for SPHK1 Gene

  • Addgene plasmids for SPHK1

Sources for SPHK1 Gene

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